##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938039_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1830304 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54660154815812 34.0 31.0 34.0 31.0 34.0 2 32.755779914156335 34.0 31.0 34.0 31.0 34.0 3 32.803695451684526 34.0 31.0 34.0 31.0 34.0 4 36.252931207056314 37.0 35.0 37.0 35.0 37.0 5 36.18877301257059 37.0 37.0 37.0 35.0 37.0 6 36.26166746070598 37.0 37.0 37.0 35.0 37.0 7 36.237847373988146 37.0 37.0 37.0 35.0 37.0 8 36.23455229295243 37.0 37.0 37.0 35.0 37.0 9 37.93103495375632 39.0 38.0 39.0 35.0 39.0 10-11 37.91304832421281 39.0 38.0 39.0 35.0 39.0 12-13 37.8514203105058 39.0 38.0 39.0 35.0 39.0 14-15 39.0784050081298 40.0 38.0 41.0 36.0 41.0 16-17 39.021210137769465 40.0 38.0 41.0 36.0 41.0 18-19 39.288195840690946 40.5 39.0 41.0 36.0 41.0 20-21 39.27244627122052 41.0 39.0 41.0 36.0 41.0 22-23 39.17662885509729 40.5 39.0 41.0 35.5 41.0 24-25 39.05172501398675 40.5 39.0 41.0 35.0 41.0 26-27 38.88192207414724 40.0 38.0 41.0 35.0 41.0 28-29 38.760533769253634 40.0 38.0 41.0 35.0 41.0 30-31 38.4826564330297 40.0 38.0 41.0 34.5 41.0 32-33 38.179920384810394 40.0 37.5 41.0 34.0 41.0 34-35 38.0192700229033 40.0 37.0 41.0 34.0 41.0 36-37 37.92272403928528 40.0 37.0 41.0 33.5 41.0 38-39 37.710867702851544 40.0 37.0 41.0 33.0 41.0 40-41 37.4987433781492 40.0 37.0 41.0 33.0 41.0 42-43 37.20668342526706 40.0 36.0 41.0 32.5 41.0 44-45 36.884515086018496 40.0 36.0 41.0 31.5 41.0 46-47 36.435119521128726 39.5 35.0 41.0 30.5 41.0 48-49 35.92487750668741 39.0 35.0 41.0 29.5 41.0 50-51 35.50100475112331 39.0 35.0 40.5 28.0 41.0 52-53 35.010264961449025 38.0 35.0 40.0 26.5 41.0 54-55 34.66712387668934 38.0 34.0 40.0 25.5 41.0 56-57 34.27388510323968 38.0 34.0 40.0 24.0 41.0 58-59 33.79655456142805 37.0 33.5 40.0 22.5 41.0 60-61 33.30638025158662 36.5 33.0 39.5 21.5 41.0 62-63 32.80920956300156 36.0 32.5 39.0 19.5 40.5 64-65 32.29259183173943 35.0 32.0 39.0 17.0 40.0 66-67 31.756172745074043 35.0 31.0 38.0 11.0 40.0 68-69 31.186577202475654 35.0 31.0 37.5 7.5 40.0 70-71 30.59453730090739 35.0 30.0 37.0 4.0 39.0 72-73 29.994079945189434 34.0 29.0 36.0 2.0 39.0 74-75 29.429606502526354 34.0 29.0 36.0 2.0 38.5 76-77 28.11183797882756 32.5 27.0 34.5 2.0 36.0 78-79 28.79281201374198 34.0 29.0 35.0 2.0 37.0 80-81 28.570740980733255 34.0 29.0 35.0 2.0 36.5 82-83 28.098434194538175 34.0 27.5 35.0 2.0 36.0 84-85 27.545648427802156 33.0 26.5 35.0 2.0 36.0 86-87 26.994538885343637 33.0 25.5 35.0 2.0 35.0 88-89 26.282554701295524 32.5 24.5 35.0 2.0 35.0 90-91 25.736332325121946 32.0 23.0 34.5 2.0 35.0 92-93 24.949369339738098 31.5 19.0 34.0 2.0 35.0 94-95 24.177152811773347 31.0 11.0 34.0 2.0 35.0 96-97 23.25429737355106 31.0 2.0 34.0 2.0 35.0 98-99 21.943223639351714 30.5 2.0 34.0 2.0 35.0 100-101 17.95182412320576 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09400701853397209 1101 2 0.1329109950235008 1101 3 0.11373905401755735 1101 4 0.04203986894697209 1101 5 -0.012488723386219647 1101 6 -0.013784017815964944 1101 7 0.05347119013413959 1101 8 0.08512212379727657 1101 9 0.024226542540127127 1101 10-11 0.10631675862457968 1101 12-13 0.1875041144639482 1101 14-15 0.30092918275664005 1101 16-17 0.2434360791053578 1101 18-19 0.20269399712990577 1101 20-21 0.256302732701009 1101 22-23 0.3329905092336318 1101 24-25 0.276018807607997 1101 26-27 0.3234117796437772 1101 28-29 0.1472238756042472 1101 30-31 0.335430194470419 1101 32-33 0.346704331033429 1101 34-35 0.04988937438731256 1101 36-37 0.019218066829019165 1101 38-39 0.18376498629094584 1101 40-41 0.32428828195656934 1101 42-43 0.39582778768336624 1101 44-45 0.3897149763968244 1101 46-47 0.4251581245930893 1101 48-49 0.45437746371628407 1101 50-51 0.33596932756169195 1101 52-53 0.37494219836261067 1101 54-55 0.6303903457405369 1101 56-57 0.4468459810766845 1101 58-59 0.5107575222598797 1101 60-61 0.5221346991743019 1101 62-63 0.5706468461228624 1101 64-65 0.6455155544028628 1101 66-67 0.7973270327332926 1101 68-69 1.1560940196595233 1101 70-71 1.1412057441898682 1101 72-73 1.2276682749852235 1101 74-75 1.3375664612463822 1101 76-77 1.0997781109522897 1101 78-79 1.036961364911381 1101 80-81 1.1604369169997675 1101 82-83 1.269865153324524 1101 84-85 1.3303405003788242 1101 86-87 1.4334411726627714 1101 88-89 1.897679117575624 1101 90-91 2.0477300203679825 1101 92-93 2.114654938489547 1101 94-95 2.4525886355687128 1101 96-97 2.8259906391622813 1101 98-99 3.0924352774115675 1101 100-101 3.0681341896056544 1102 1 0.1833306868050073 1102 2 0.21200781096241883 1102 3 0.1705935319246663 1102 4 0.07406995360863533 1102 5 0.0423222787932076 1102 6 0.03323487130818137 1102 7 0.11556698907204321 1102 8 0.13794365030301492 1102 9 0.12839738355307162 1102 10-11 0.1870927867690355 1102 12-13 0.23759319086881447 1102 14-15 0.2909109885279193 1102 16-17 0.3480621310998302 1102 18-19 0.3535782006456927 1102 20-21 0.21486550705326124 1102 22-23 0.18202246352874596 1102 24-25 0.23655542603935942 1102 26-27 0.3214867250871478 1102 28-29 0.17340016493842114 1102 30-31 0.27905863809886 1102 32-33 0.2761319446715689 1102 34-35 0.29175656362291846 1102 36-37 0.3117242803878568 1102 38-39 0.2582578239416833 1102 40-41 0.4446754812911351 1102 42-43 0.37493237205111285 1102 44-45 0.4215413766442708 1102 46-47 0.40839063414139076 1102 48-49 0.5481708206675364 1102 50-51 0.4613700055939418 1102 52-53 0.4433834773302081 1102 54-55 0.5331357748017496 1102 56-57 0.4552320947259574 1102 58-59 0.5679184741576719 1102 60-61 0.6073825475800447 1102 62-63 0.744593572701163 1102 64-65 0.7892606192005616 1102 66-67 0.785933258181629 1102 68-69 0.8439095232645037 1102 70-71 0.8015013518538971 1102 72-73 1.0018942860533393 1102 74-75 1.02556528082415 1102 76-77 0.994556637309767 1102 78-79 1.222906287698411 1102 80-81 1.2932588129795555 1102 82-83 1.318897676041253 1102 84-85 1.4411043216689592 1102 86-87 1.523002867487623 1102 88-89 1.537202230782949 1102 90-91 1.7784255931688158 1102 92-93 1.9062213616349162 1102 94-95 2.4247741077541853 1102 96-97 2.397769974099507 1102 98-99 2.6178659870528023 1102 100-101 3.244609445028278 1103 1 0.14091759062439735 1103 2 0.11027538965408468 1103 3 0.2145015360750051 1103 4 0.05420341348461477 1103 5 -0.053292821744271635 1103 6 -0.04806058446663286 1103 7 0.09698882357983507 1103 8 0.006701548973829574 1103 9 0.167279743147283 1103 10-11 0.128196363237997 1103 12-13 0.1709935655793018 1103 14-15 0.3447006294057857 1103 16-17 0.35330660264494185 1103 18-19 0.17090683944346807 1103 20-21 0.13149734170856675 1103 22-23 0.14677410718989137 1103 24-25 0.24095655312128628 1103 26-27 0.29776735471877913 1103 28-29 0.3513261768288416 1103 30-31 0.4292968890565376 1103 32-33 0.2722348572833724 1103 34-35 0.3208261309355791 1103 36-37 0.23954333751400014 1103 38-39 0.3305125287219468 1103 40-41 0.3817074005267713 1103 42-43 0.4591365056683472 1103 44-45 0.5135323088472461 1103 46-47 0.39658872911721943 1103 48-49 0.5219017470679077 1103 50-51 0.6949837980869447 1103 52-53 0.7116702832921362 1103 54-55 0.8823074285928669 1103 56-57 0.8578049100212297 1103 58-59 0.871315947566508 1103 60-61 0.7945583025331544 1103 62-63 0.7483652648484735 1103 64-65 0.8336357820914344 1103 66-67 0.8811549618849597 1103 68-69 1.1800499069247685 1103 70-71 1.120442917671646 1103 72-73 1.3238281265407572 1103 74-75 1.2512890080624537 1103 76-77 1.2841876802539467 1103 78-79 1.2467461358472356 1103 80-81 1.3036149846382656 1103 82-83 1.4131998146447948 1103 84-85 1.72396648033574 1103 86-87 1.8356476252289369 1103 88-89 2.1075391259967873 1103 90-91 2.1627939699643086 1103 92-93 2.315351610409241 1103 94-95 2.442759719264977 1103 96-97 2.808107459408596 1103 98-99 3.2168839702055827 1103 100-101 3.16940180526176 1104 1 0.14897803870076842 1104 2 0.12810349114825215 1104 3 0.17761614524082603 1104 4 0.10794298581341621 1104 5 -0.028421278784279025 1104 6 0.07679750408935604 1104 7 0.1137092739893788 1104 8 0.07157738433581784 1104 9 0.17048888271173013 1104 10-11 0.2095829066390209 1104 12-13 0.2110446028894586 1104 14-15 0.2740802513085683 1104 16-17 0.30543961345195925 1104 18-19 0.19018423632928005 1104 20-21 0.3213629643243223 1104 22-23 0.27134359674769115 1104 24-25 0.24522334704249715 1104 26-27 0.2513323856252967 1104 28-29 0.2477559226869417 1104 30-31 0.1742343138836162 1104 32-33 0.17277429352352414 1104 34-35 0.18299633036561858 1104 36-37 0.1724956930712196 1104 38-39 0.20415596100137634 1104 40-41 0.22037263422488707 1104 42-43 0.29422372150502696 1104 44-45 0.19211696245324816 1104 46-47 0.2786037679703668 1104 48-49 0.3640252910148263 1104 50-51 0.531605059866159 1104 52-53 0.5740569319708229 1104 54-55 0.7870388669011774 1104 56-57 0.8371080938372444 1104 58-59 0.8810494568774843 1104 60-61 0.8626605527517412 1104 62-63 0.9122675460406455 1104 64-65 1.0465919666663552 1104 66-67 1.0813522253973957 1104 68-69 1.172392887729675 1104 70-71 1.1945329873865944 1104 72-73 1.4506633214731188 1104 74-75 1.3094439418249202 1104 76-77 1.3587626775813568 1104 78-79 1.4843887128980846 1104 80-81 1.639425730357754 1104 82-83 1.7329459613400715 1104 84-85 1.9245257120282986 1104 86-87 1.95618120006408 1104 88-89 2.032461722526037 1104 90-91 2.2605723267466438 1104 92-93 2.519634378894718 1104 94-95 2.638232495233705 1104 96-97 3.175716733760968 1104 98-99 3.6471913769159556 1104 100-101 3.738781410905453 1105 1 0.1506566979273316 1105 2 0.25133405568482914 1105 3 0.24183785540156322 1105 4 0.16885524305476451 1105 5 -0.005711326717126042 1105 6 0.10591246289134659 1105 7 0.12484025428298651 1105 8 0.09438381536944007 1105 9 0.1550335506034486 1105 10-11 0.18842858441871613 1105 12-13 0.27664039042333144 1105 14-15 0.34646770934209314 1105 16-17 0.3270965237766319 1105 18-19 0.31231632426126055 1105 20-21 0.2543213011166898 1105 22-23 0.34954559528330975 1105 24-25 0.31258772865345463 1105 26-27 0.2832558920149708 1105 28-29 0.43885457878729284 1105 30-31 0.28829488921236646 1105 32-33 0.34871453340463887 1105 34-35 0.4758677687772561 1105 36-37 0.44387550682941423 1105 38-39 0.44082821447330645 1105 40-41 0.42612936213699726 1105 42-43 0.4526169428479747 1105 44-45 0.5112577910804319 1105 46-47 0.3466908069560901 1105 48-49 0.4251088171191171 1105 50-51 0.5609844009522149 1105 52-53 0.4627093601369907 1105 54-55 0.493956147031966 1105 56-57 0.5297246108867242 1105 58-59 0.5885312137519847 1105 60-61 0.6432434502469491 1105 62-63 0.7780929759516653 1105 64-65 0.7931084402628983 1105 66-67 0.8624584651932423 1105 68-69 1.2538233791217372 1105 70-71 1.3535622967991259 1105 72-73 1.2182877475541538 1105 74-75 1.090462303492405 1105 76-77 1.1512027652650296 1105 78-79 1.172827653304747 1105 80-81 1.2382418810863207 1105 82-83 1.2395063440848446 1105 84-85 1.398929430881111 1105 86-87 1.424018739955141 1105 88-89 1.383006641711816 1105 90-91 1.748652686994621 1105 92-93 2.115151547289404 1105 94-95 2.131343410893887 1105 96-97 2.545782563827853 1105 98-99 2.783076765886989 1105 100-101 2.8897840816526568 1106 1 0.08926734557543625 1106 2 0.058191160117402774 1106 3 0.043424089639728436 1106 4 0.01909178429923486 1106 5 -0.09596263340262112 1106 6 0.03298183104534047 1106 7 0.02051694996220732 1106 8 0.031321793512667284 1106 9 0.07982996543109522 1106 10-11 0.09942547583109018 1106 12-13 0.06045486139606737 1106 14-15 -0.051313832175551966 1106 16-17 -0.09653259468171171 1106 18-19 -0.08166318623775481 1106 20-21 -0.19360215113868406 1106 22-23 -0.0723709281417726 1106 24-25 -0.0563329092023821 1106 26-27 -0.236963586319483 1106 28-29 -0.086583586375518 1106 30-31 0.09104176556510879 1106 32-33 0.03869980999049005 1106 34-35 -0.20275274117680198 1106 36-37 -0.20516894219129256 1106 38-39 -0.1495344634229383 1106 40-41 -0.10463255703411534 1106 42-43 -0.2276951487033685 1106 44-45 -0.21041434608407172 1106 46-47 -0.07065039100587711 1106 48-49 -0.10221705504354617 1106 50-51 0.005914839474606026 1106 52-53 -0.1035025757748329 1106 54-55 -0.002989900270677026 1106 56-57 0.03572674865360881 1106 58-59 0.16769232936930933 1106 60-61 0.03231632344207469 1106 62-63 0.10442498263674338 1106 64-65 0.2414041805592433 1106 66-67 0.31081654534969516 1106 68-69 -0.037215861368633085 1106 70-71 0.06039754725827251 1106 72-73 0.24638131057834656 1106 74-75 0.508015386933959 1106 76-77 0.4442708530003294 1106 78-79 0.45839009192800617 1106 80-81 0.40723753690865294 1106 82-83 0.6171296964563524 1106 84-85 0.7631272989979863 1106 86-87 0.7390409651659411 1106 88-89 0.9550463038717965 1106 90-91 1.0191239763370241 1106 92-93 1.1761206309214778 1106 94-95 1.300290035365574 1106 96-97 1.6041810037903623 1106 98-99 1.9485589752116326 1106 100-101 2.2281431284271633 1107 1 0.26117423015845986 1107 2 0.23212252668307087 1107 3 0.25982656095956713 1107 4 0.11263871437260775 1107 5 0.05732869573836297 1107 6 0.15174471930630062 1107 7 0.10490128428941148 1107 8 0.08908607175661132 1107 9 0.251598389426988 1107 10-11 0.2986282520330761 1107 12-13 0.2826978825791926 1107 14-15 0.37895277838993024 1107 16-17 0.3027786647181472 1107 18-19 0.32959634805073534 1107 20-21 0.30236367194901703 1107 22-23 0.27841930541300997 1107 24-25 0.3712585890487077 1107 26-27 0.38080420048886054 1107 28-29 0.27957140344830833 1107 30-31 0.3009386057063068 1107 32-33 0.36227884141163 1107 34-35 0.4877975926073077 1107 36-37 0.5098697595592299 1107 38-39 0.5936550904651128 1107 40-41 0.706766647691289 1107 42-43 0.6658269794492995 1107 44-45 0.6957029905257102 1107 46-47 0.6432466830396137 1107 48-49 0.6342991690421442 1107 50-51 0.776118994905346 1107 52-53 0.9042854961816644 1107 54-55 0.9183547402291765 1107 56-57 0.9368490859758651 1107 58-59 1.042196627024623 1107 60-61 1.0617099601698854 1107 62-63 0.8787990870118279 1107 64-65 0.914335404631494 1107 66-67 1.1461472510685269 1107 68-69 1.3016687781095762 1107 70-71 1.270524594929043 1107 72-73 1.4742524857726629 1107 74-75 1.358040390496292 1107 76-77 1.2052522562647745 1107 78-79 1.3856569471520004 1107 80-81 1.4538412869052912 1107 82-83 1.5710283701921277 1107 84-85 1.5866401247500406 1107 86-87 1.8261609042970548 1107 88-89 1.9379749872594871 1107 90-91 2.3678571291786916 1107 92-93 2.313581747693494 1107 94-95 2.5056580044579455 1107 96-97 2.7655635394742113 1107 98-99 2.8416062373215567 1107 100-101 2.894331456334097 1108 1 0.25940820567429057 1108 2 0.18770324546792239 1108 3 0.1456170858955943 1108 4 0.17041071470912073 1108 5 0.09540847146750053 1108 6 0.12635277449474103 1108 7 0.15529709630787636 1108 8 0.16441044221603107 1108 9 0.23527538402375114 1108 10-11 0.15879649588692502 1108 12-13 0.16911699825074322 1108 14-15 0.3167175463710876 1108 16-17 0.3134592795633395 1108 18-19 0.3232459872471125 1108 20-21 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0.704827532571958 2316 80-81 0.7393109105530371 2316 82-83 0.9443409005798742 2316 84-85 1.2458719224891937 2316 86-87 1.398571583311277 2316 88-89 1.7176106381650413 2316 90-91 1.7534154470649206 2316 92-93 1.6926565547312933 2316 94-95 2.0965743469886355 2316 96-97 2.5666572135048433 2316 98-99 2.753514056089031 2316 100-101 3.3744208342969024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 12.0 5 40.0 6 93.0 7 244.0 8 459.0 9 1029.0 10 1826.0 11 3022.0 12 4585.0 13 6868.0 14 8746.0 15 12398.0 16 16964.0 17 24597.0 18 24950.0 19 18380.0 20 16033.0 21 15604.0 22 16597.0 23 17796.0 24 20088.0 25 22450.0 26 24590.0 27 28876.0 28 34314.0 29 42115.0 30 51130.0 31 63205.0 32 78863.0 33 101429.0 34 138958.0 35 206079.0 36 287489.0 37 353707.0 38 179035.0 39 7732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35470828889627 18.882874101788556 15.185291623686556 21.577125985628616 2 15.779994284174869 21.31439934382765 38.46105506700248 24.444551304995006 3 19.53681788081641 25.06015565069728 31.16330428815267 24.239722180333636 4 12.634987135756695 17.378229515147243 37.093058007383625 32.89372534171244 5 12.59065081500259 38.425004827598656 34.16687134955589 14.817473007842866 6 30.761060612434786 37.03494671095082 16.384791353990412 15.819201322623982 7 27.045507194433277 35.01959237372589 20.30761010192842 17.627290329912405 8 27.12895543296478 32.27024655534424 19.804141290576023 20.796656721114964 9 26.5753667150375 16.76825270556148 20.652088396244558 36.00429218315646 10-11 24.91392140322045 26.402144124691855 26.585255782645945 22.098678689441755 12-13 27.137404496739336 24.46459713796178 26.988440171687326 21.409558193611552 14-15 23.10097120478347 25.753372117418742 26.82084506180394 24.324811615993845 16-17 20.882104830672937 28.996385299928317 27.957678068779828 22.16383180061891 18-19 21.047924279245414 26.84638726681469 31.098659020578 21.00702943336189 20-21 21.850823688305333 25.272413762959594 31.430598414252497 21.44616413448258 22-23 21.471392265914805 25.54534077874098 31.917381872539508 21.06588508280471 24-25 21.953840711252436 25.967680029284935 31.378782353093836 20.699696906368793 26-27 20.8383464521325 25.838843666095144 32.027245139596914 21.295564742175436 28-29 20.834735650471178 27.317839003793903 31.13217804255468 20.715247303180238 30-31 20.85336643530255 26.058703909295943 30.82731065440495 22.260619000996556 32-33 20.46354048289246 26.457735982656434 31.10010686749305 21.978616666958057 34-35 22.22043725942276 26.221877615118487 30.47136405126814 21.08632107419061 36-37 22.038350679822663 25.44897853814156 31.18163835472289 21.331032427312888 38-39 21.086643235423885 26.26832643281867 30.851433809015795 21.793596522741645 40-41 21.438400981355073 26.318921274395567 30.978855240564496 21.263822503684864 42-43 21.218165416623183 26.44455287200382 29.936050809664284 22.40123090170872 44-45 21.200275592573206 26.068850062123065 30.758294822859106 21.972579522444622 46-47 22.498257120128677 26.878977481336435 29.517910685875133 21.104854712659755 48-49 21.575596185114605 26.909573491616694 29.231100407364025 22.28372991590468 50-51 21.079531050579575 27.20837631344301 29.98775066874137 21.724341967236043 52-53 22.173584278895746 27.0573904662832 29.0944837578894 21.674541496931656 54-55 20.992988050072555 27.00362890536217 29.518429725335242 22.48495331923003 56-57 21.757560492683183 27.00586897040054 28.79117348812001 22.445397048796263 58-59 22.00429546130042 26.56689817647779 28.706624691854465 22.722181670367327 60-61 20.857573386716087 27.358679213944786 29.734951133800724 22.048796265538403 62-63 20.836156179519904 27.55416586534259 29.67436010629928 21.935317848838228 64-65 20.364677124674373 28.170129115163384 28.842749619735304 22.622444140426946 66-67 20.579078983097336 27.475560057542385 28.614114712154215 23.331246247206064 68-69 21.323931549682538 26.970711214774045 28.95875573884153 22.746601496701892 70-71 21.57485860272392 27.23782497333776 28.06372602584052 23.1235903980978 72-73 21.17534637707527 26.995940016489072 28.877131272800188 22.95158233363547 74-75 20.916579977970873 28.460080948301485 27.960355219679357 22.66298385404829 76-77 21.148098895046942 27.05960321345525 28.593993129010265 23.198304762487542 78-79 21.173668417924016 27.5632627148277 28.498626457681347 22.764442409566936 80-81 22.020397715569498 27.46690028918709 27.92499383981778 22.58770815542563 82-83 21.45651615074594 27.254617689233534 28.593052997874906 22.69581316214562 84-85 21.071222898155746 27.732968496786736 28.37155083579842 22.824257769259095 86-87 21.578734857210296 27.907453869538706 27.591512388512136 22.922298884738865 88-89 20.965315051488716 27.972894120320994 26.942819334930153 24.118971493260137 90-91 21.12145151517304 27.30655270063756 27.600118672118008 23.97187711207139 92-93 21.649299788450442 27.22957497770862 27.891541514415092 23.229583719425843 94-95 21.06211375930652 26.525608055059735 28.323780270261263 24.088497915372482 96-97 20.729092794522433 26.242472553709717 28.852588261390743 24.175846390377107 98-99 20.783487333251742 27.099569251883842 27.487018549923945 24.62992486494047 100-101 20.829135487875238 25.717722301868978 28.264566979037365 25.18857523121842 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3073.0 1 2030.0 2 1269.0 3 1535.5 4 1288.0 5 1313.5 6 2385.0 7 4888.5 8 7591.0 9 8327.5 10 6476.0 11 4441.0 12 4045.0 13 4091.0 14 4127.5 15 4077.0 16 4100.0 17 4296.5 18 4403.0 19 4702.5 20 5134.0 21 5683.0 22 6587.0 23 7579.5 24 8692.0 25 10348.0 26 12727.5 27 15356.0 28 17228.0 29 19234.5 30 22129.0 31 26869.5 32 34984.5 33 36852.0 34 32727.0 35 32948.0 36 36314.5 37 39832.0 38 42235.0 39 43284.5 40 44971.5 41 48067.0 42 49551.0 43 51275.5 44 53838.0 45 58700.5 46 61118.5 47 68633.5 48 84007.5 49 80847.0 50 66737.0 51 59942.0 52 57038.0 53 57808.0 54 60230.5 55 57787.0 56 52705.5 57 48265.0 58 46056.0 59 41635.0 60 32746.0 61 27476.5 62 24680.0 63 18172.5 64 11325.5 65 8273.0 66 6212.5 67 4941.5 68 3900.5 69 2861.5 70 2106.5 71 1371.0 72 985.5 73 754.5 74 529.0 75 381.5 76 270.5 77 183.0 78 122.0 79 60.5 80 28.0 81 17.0 82 7.0 83 5.0 84 2.5 85 1.5 86 1.5 87 1.0 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016226812595066174 3 0.0028410580974526636 4 0.0020215221078028565 5 0.12418702029826738 6 1.6390719792996135E-4 7 0.0 8 2.7317866321660225E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.912250642516216E-4 24-25 6.283109253981853E-4 26-27 0.005791387660191968 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010954464394985751 36-37 5.736751927548647E-4 38-39 0.006911420179380037 40-41 0.009916385474762663 42-43 0.005518208996975366 44-45 0.0036059583544591502 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.4635732643320456E-5 68-69 7.102645243631659E-4 70-71 0.0 72-73 5.4635732643320456E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.4635732643320456E-5 82-83 0.0017756613109079149 84-85 0.004261587146178996 86-87 0.003278143958599227 88-89 0.0 90-91 0.002786422364809343 92-93 0.0 94-95 5.4635732643320456E-5 96-97 1.0927146528664091E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1830304.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.32426550565104 #Duplication Level Percentage of deduplicated Percentage of total 1 66.92184136178088 24.978085751141688 2 15.517154547588369 11.583327924528176 3 6.544290765063819 7.327825381842665 4 3.3877667230424424 5.057836185681819 5 1.9896178302991285 3.713051207643101 6 1.2761210766672888 2.8578169129732296 7 0.8363251570773479 2.1850655548267173 8 0.6245175480128091 1.8647727019974605 9 0.43284399690646763 1.4540025836757775 >10 2.19999449728569 14.791232707505802 >50 0.16209002828455912 4.181257928780313 >100 0.0885033270073144 6.524079722898238 >500 0.007044889668407716 1.824128463536476 >1k 0.011007640106887056 8.175244286999401 >5k 4.4030560427548224E-4 1.1754204162918456 >10k+ 4.4030560427548224E-4 2.306852269677304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16572 0.9054233613651066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13681 0.7474714582932671 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11858 0.6478705176844939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9208 0.5030858261796947 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7052 0.38529118660069583 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5197 0.2839419025473364 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4111 0.2246074968966904 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3710 0.2026985681067189 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3706 0.2024800251761456 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3549 0.19390221515114428 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3475 0.18985917093553856 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3360 0.1835760616815567 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3250 0.17756613109079147 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3145 0.17182937916324284 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3085 0.1685512352046436 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3064 0.16740388481913387 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 3028 0.16543699844397433 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3005 0.16418037659317797 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2921 0.15959097505113903 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2873 0.15696845988425967 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.15560256656817667 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2842 0.15527475217231673 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2777 0.15172342955050092 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2731 0.14921018584890816 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2728 0.1490462786509782 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2698 0.1474072066716786 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2688 0.14686084934524538 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2683 0.14658767068202877 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2366 0.1292681434340962 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2297 0.1254982778817071 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2270 0.12402311310033742 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2204 0.12041715474587827 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 2172 0.11866881130129202 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2082 0.11375159536339317 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 2049 0.11194861618616361 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1999 0.10921682955399759 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1978 0.10806947916848786 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1882 0.1028244488347291 RNA PCR Primer, Index 31 (95% over 22bp) TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 1877 0.1025512701715125 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 1850 0.10107610539014285 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4635732643320456E-5 2 0.0 0.0 0.0 0.0 5.4635732643320456E-5 3 0.0 0.0 0.0 0.0 1.0927146528664091E-4 4 0.0 0.0 0.0 0.0 1.0927146528664091E-4 5 0.0 0.0 0.0 0.0 1.0927146528664091E-4 6 0.0 0.0 0.0 0.0 1.0927146528664091E-4 7 0.0 0.0 0.0 0.0 1.0927146528664091E-4 8 0.0 0.0 0.0 0.0 1.0927146528664091E-4 9 0.0 0.0 0.0 0.0 1.0927146528664091E-4 10-11 0.0 0.0 0.0 2.7317866321660228E-5 1.0927146528664091E-4 12-13 0.0 0.0 0.0 5.4635732643320456E-5 4.3708586114656365E-4 14-15 0.0 0.0 0.0 5.4635732643320456E-5 5.736751927548647E-4 16-17 0.0 0.0 0.0 3.2781439585992275E-4 6.556287917198454E-4 18-19 0.0 0.0 0.0 5.190394601115444E-4 7.375823906848262E-4 20-21 0.0 0.0 0.0 6.829466580415056E-4 9.288074549364477E-4 22-23 0.0 0.0 0.0 0.0013932111824046715 0.0010380789202230887 24-25 0.0 0.0 0.0 0.00363327622078081 0.001092714652866409 26-27 0.0 0.0 0.0 0.008222677762819729 0.001092714652866409 28-29 0.0 0.0 0.0 0.019750817350560345 0.0011473503855097295 30-31 0.0 0.0 0.0 0.051985899610119415 0.0011473503855097295 32-33 0.0 0.0 0.0 0.11487162788258126 0.0012566218507963705 34-35 0.0 0.0 0.0 0.20272588597304053 0.0013112575834396908 36-37 0.0 0.0 0.0 0.32306108711995385 0.0013112575834396908 38-39 0.0 0.0 0.0 0.49647490252985294 0.0013112575834396908 40-41 0.0 0.0 0.0 0.6814441753938144 0.0013112575834396908 42-43 0.0 0.0 0.0 0.8649109656100844 0.0013112575834396908 44-45 0.0 0.0 0.0 1.0882072049233351 0.001338575449761351 46-47 0.0 0.0 0.0 1.3402418396069724 0.0013658933160830113 48-49 0.0 0.0 0.0 1.5774155550116267 0.0014751647813696523 50-51 0.0 0.0 0.0 1.8374543245275101 0.0014751647813696523 52-53 0.0 0.0 0.0 2.074081682605731 0.001584436246656293 54-55 0.0 0.0 0.0 2.3251328741017883 0.0016390719792996136 56-57 0.0 0.0 0.0 2.6237444708638566 0.0016390719792996136 58-59 0.0 0.0 0.0 2.908942995261989 0.0016390719792996136 60-61 0.0 0.0 0.0 3.221896471842929 0.0016390719792996136 62-63 0.0 0.0 0.0 3.520753929401892 0.0016390719792996136 64-65 0.0 0.0 0.0 3.821988041330839 0.0016390719792996136 66-67 0.0 0.0 0.0 4.180480401069985 0.0016390719792996136 68-69 0.0 0.0 0.0 4.559679703480952 0.0016390719792996136 70-71 0.0 0.0 0.0 4.937294569645261 0.0016390719792996136 72-73 0.0 0.0 0.0 5.319744698148504 0.001693707711942934 74-75 0.0 0.0 0.0 5.708505253772051 0.0017483434445862546 76-77 0.0 0.0 0.0 6.106717791142891 0.0017483434445862546 78-79 0.0 0.0 0.0 6.582157936605066 0.0017483434445862546 80-81 0.0 0.0 0.0 7.060165961501477 0.0017483434445862546 82-83 0.0 0.0 0.0 7.5433916988653245 0.0018849327761945556 84-85 0.0 0.0 0.0 8.039511469132997 0.001912250642516216 86-87 0.0 0.0 0.0 8.529566673077259 0.0019942042414811964 88-89 0.0 0.0 0.0 9.042650838330681 0.0020761578404461774 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 1140 0.0 30.876516 2 TAAGGTG 1465 0.0 29.5424 5 GTAAGGT 1480 0.0 27.958344 4 TAAGGTA 580 0.0 27.060751 4 AAGGTAA 1305 0.0 26.608095 1 GTACATG 9145 0.0 25.694765 1 GGTAAGG 1575 0.0 25.366035 3 TACATGG 9165 0.0 25.01589 2 ACATGGG 8985 0.0 23.55569 3 GGTGTGC 1815 0.0 23.551268 8 GAGTACT 11865 0.0 23.337225 12-13 GTGTAAG 595 0.0 23.183683 1 GTACTTT 12145 0.0 22.56455 14-15 AAGGTGT 1945 0.0 22.465528 6 AGGTGTG 1920 0.0 22.015936 7 CGTATAG 135 3.7614122E-4 21.140678 1 AGTACTT 11960 0.0 21.126564 12-13 CATGGGA 5090 0.0 21.024158 4 AGAGTAC 16705 0.0 20.868824 10-11 GTGTGCA 2015 0.0 20.270844 9 >>END_MODULE