##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938038_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817038 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5628335786043 34.0 31.0 34.0 31.0 34.0 2 32.777161512307394 34.0 31.0 34.0 31.0 34.0 3 32.7163548588417 34.0 31.0 34.0 31.0 34.0 4 36.2312472276309 37.0 35.0 37.0 35.0 37.0 5 36.21125700177982 37.0 37.0 37.0 35.0 37.0 6 36.28736107885471 37.0 37.0 37.0 35.0 37.0 7 36.25296664131405 37.0 37.0 37.0 35.0 37.0 8 36.249228689768735 37.0 37.0 37.0 35.0 37.0 9 37.963975436947386 39.0 38.0 39.0 35.0 39.0 10-11 37.93305093234154 39.0 38.0 39.0 35.0 39.0 12-13 37.88985315662083 39.0 38.0 39.0 35.0 39.0 14-15 39.13965090438395 40.5 38.5 41.0 36.0 41.0 16-17 39.10662187582208 40.0 38.5 41.0 36.0 41.0 18-19 39.15376728499899 40.0 39.0 41.0 36.0 41.0 20-21 39.17279275392149 40.5 39.0 41.0 36.0 41.0 22-23 39.18115691581574 41.0 39.0 41.0 35.5 41.0 24-25 39.10437453702124 40.5 39.0 41.0 35.0 41.0 26-27 38.92232413411277 40.0 38.0 41.0 35.0 41.0 28-29 38.83931761471142 40.0 38.0 41.0 35.0 41.0 30-31 38.5792581112778 40.0 38.0 41.0 35.0 41.0 32-33 38.29934156577903 40.0 38.0 41.0 34.0 41.0 34-35 38.04803091624942 40.0 37.0 41.0 34.0 41.0 36-37 37.910385748674486 40.0 37.0 41.0 33.5 41.0 38-39 37.80627592818642 40.0 37.0 41.0 33.0 41.0 40-41 37.67626351237564 40.0 37.0 41.0 33.0 41.0 42-43 37.43688051653295 40.0 37.0 41.0 33.0 41.0 44-45 37.131666206210326 40.0 36.0 41.0 32.5 41.0 46-47 36.68540696452138 40.0 36.0 41.0 31.0 41.0 48-49 36.20970667647016 39.5 35.0 41.0 30.0 41.0 50-51 35.84607641667373 39.0 35.0 41.0 29.0 41.0 52-53 35.40532806688688 39.0 35.0 40.5 27.5 41.0 54-55 35.08970808535649 38.5 35.0 40.0 26.5 41.0 56-57 34.77436602866864 38.0 35.0 40.0 26.0 41.0 58-59 34.38783393632934 38.0 34.0 40.0 25.5 41.0 60-61 33.936095172473 37.0 34.0 40.0 23.5 41.0 62-63 33.52887171319477 36.5 33.5 39.5 22.5 41.0 64-65 33.09274021787107 36.0 33.0 39.0 21.5 41.0 66-67 32.57068756955 35.5 32.5 39.0 19.5 40.0 68-69 32.03968629164608 35.0 32.0 38.0 17.0 40.0 70-71 31.436491696926538 35.0 31.0 37.0 10.5 39.5 72-73 31.007785748014076 35.0 31.0 37.0 7.5 39.0 74-75 30.520422247635988 34.5 30.5 36.0 7.0 39.0 76-77 29.287911149904403 33.5 28.5 35.0 3.5 37.0 78-79 29.96795829256185 34.0 30.0 35.0 2.0 37.0 80-81 29.826544354053134 34.0 30.0 35.0 2.0 37.0 82-83 29.435920162374153 34.0 30.0 35.0 2.0 36.5 84-85 29.017083021929096 34.0 29.0 35.0 2.0 36.0 86-87 28.61522021003413 34.0 29.0 35.0 2.0 35.5 88-89 28.074424695575985 34.0 28.0 35.0 2.0 35.0 90-91 27.642197906703107 33.0 27.0 35.0 2.0 35.0 92-93 27.147150747535274 33.0 26.0 35.0 2.0 35.0 94-95 26.528992239017565 33.0 25.0 35.0 2.0 35.0 96-97 25.85531865596647 32.5 23.5 35.0 2.0 35.0 98-99 24.998984336045808 32.0 17.5 34.0 2.0 35.0 100-101 21.57528901431891 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0279781793274978 1101 2 0.10114663232240417 1101 3 0.12298246952308034 1101 4 0.006554068861994722 1101 5 0.019494834614995682 1101 6 0.04964499885966944 1101 7 0.06593810935189737 1101 8 0.11472899885701793 1101 9 0.13687799423910718 1101 10-11 0.20710365480054094 1101 12-13 0.11590854723928601 1101 14-15 0.21355006190192682 1101 16-17 0.14215486098932928 1101 18-19 0.2052295564703499 1101 20-21 0.16421802891127868 1101 22-23 0.12947451274931154 1101 24-25 0.06126408948756534 1101 26-27 0.11124014483411315 1101 28-29 0.0896354348055226 1101 30-31 0.13363189155582944 1101 32-33 0.07148955272802482 1101 34-35 0.14707033940869962 1101 36-37 0.17884147709030174 1101 38-39 0.20879307751345522 1101 40-41 0.24186414878928986 1101 42-43 0.2232939967946166 1101 44-45 0.2872155797266416 1101 46-47 0.3473812330324577 1101 48-49 0.3197909517667128 1101 50-51 0.27896404336086533 1101 52-53 0.37928456136814503 1101 54-55 0.4304166816879871 1101 56-57 0.433196809812074 1101 58-59 0.5496605922391709 1101 60-61 0.5351368831370777 1101 62-63 0.6649919519031471 1101 64-65 0.7477598436824593 1101 66-67 0.5189432130828706 1101 68-69 0.5259652295379666 1101 70-71 0.6268259317134728 1101 72-73 0.6546204224554621 1101 74-75 0.7460503739531532 1101 76-77 0.6116398501062505 1101 78-79 0.6259819440322012 1101 80-81 0.7085704037745373 1101 82-83 0.7804468765059909 1101 84-85 0.8290387006726156 1101 86-87 0.9900535467731473 1101 88-89 0.9653332846969747 1101 90-91 0.9955321861430555 1101 92-93 1.239050241099708 1101 94-95 1.3520845284720941 1101 96-97 1.4391171221703303 1101 98-99 1.5863273771604582 1101 100-101 1.82550018993388 1102 1 0.10956716321025084 1102 2 0.12996919485995306 1102 3 0.2634135603134169 1102 4 0.06540968667562197 1102 5 0.06412937938402052 1102 6 -0.004456891104979377 1102 7 0.12191548196199875 1102 8 0.1373002340536047 1102 9 0.21700188924754116 1102 10-11 0.1740266346139876 1102 12-13 0.21539758166127854 1102 14-15 0.17778204922532126 1102 16-17 0.2219198725559366 1102 18-19 0.29839379799975774 1102 20-21 0.20555211266285767 1102 22-23 0.1888821777396359 1102 24-25 0.22819998910491535 1102 26-27 0.2623296516311555 1102 28-29 0.1325992715988491 1102 30-31 0.10720160381998056 1102 32-33 0.18973214695709828 1102 34-35 0.15435145111982962 1102 36-37 0.2910066968937457 1102 38-39 0.2789773789383929 1102 40-41 0.26170913647536764 1102 42-43 0.1827686674346154 1102 44-45 0.0790313061679484 1102 46-47 0.2870702373082352 1102 48-49 0.10918569273097489 1102 50-51 0.2988455527148446 1102 52-53 0.11137891646938414 1102 54-55 0.11697780566026239 1102 56-57 0.1754241333395825 1102 58-59 0.2842407429448812 1102 60-61 0.6300740088691512 1102 62-63 0.4531854888767981 1102 64-65 0.43272140304367923 1102 66-67 0.5437008928718612 1102 68-69 0.6609868913202988 1102 70-71 0.759043447167624 1102 72-73 0.7890769483708588 1102 74-75 0.8358708773441812 1102 76-77 0.7705276829864722 1102 78-79 0.723625473131527 1102 80-81 0.8546868416072719 1102 82-83 0.8809646397586057 1102 84-85 0.8087237867856416 1102 86-87 0.9121082853645603 1102 88-89 1.1306867194437302 1102 90-91 1.3786153495697064 1102 92-93 1.3902611349432519 1102 94-95 1.2203762052895364 1102 96-97 1.3658312233763041 1102 98-99 1.8254845583970152 1102 100-101 2.475269638988685 1103 1 0.24714740963648296 1103 2 0.20500830034360717 1103 3 0.2786807358859349 1103 4 0.21996195972811705 1103 5 0.11992562624447345 1103 6 0.08298149000201249 1103 7 0.16260146862912706 1103 8 0.10786774467499782 1103 9 0.16687236004302264 1103 10-11 0.2184635403216575 1103 12-13 0.17022593696111699 1103 14-15 0.2971196492095274 1103 16-17 0.3613046458428144 1103 18-19 0.31252235018398977 1103 20-21 0.29715763332091427 1103 22-23 0.26214586959336117 1103 24-25 0.37110598297633146 1103 26-27 0.521138130982969 1103 28-29 0.4358769772788875 1103 30-31 0.3719511560190156 1103 32-33 0.43131274866343006 1103 34-35 0.4757765684362809 1103 36-37 0.5046844874462479 1103 38-39 0.4738884848957454 1103 40-41 0.6096721972134631 1103 42-43 0.6161176265132582 1103 44-45 0.747854736890794 1103 46-47 0.6725503835652091 1103 48-49 0.6037938237109088 1103 50-51 0.4407135858940592 1103 52-53 0.5672834744170459 1103 54-55 0.6851484091923368 1103 56-57 0.5797916113936381 1103 58-59 0.6408388372618887 1103 60-61 0.6612690823183769 1103 62-63 0.5783686801803256 1103 64-65 0.5484538968632862 1103 66-67 0.6142758051620874 1103 68-69 0.8338301683845017 1103 70-71 0.7142806213091539 1103 72-73 0.7800289016712618 1103 74-75 0.8103412879394192 1103 76-77 0.4303094817893758 1103 78-79 0.8307742516094478 1103 80-81 0.8071481432154037 1103 82-83 0.6893344468711149 1103 84-85 0.9521496245311951 1103 86-87 0.9909348247883116 1103 88-89 1.1305675525755738 1103 90-91 1.1879389850720585 1103 92-93 1.3559615548645958 1103 94-95 1.3262669467887171 1103 96-97 1.5689833474085404 1103 98-99 1.805686574611606 1103 100-101 2.0673547877869005 1104 1 0.12627148016400014 1104 2 0.12857891327844584 1104 3 0.17684112215087566 1104 4 0.03527499077497254 1104 5 0.020536133441986237 1104 6 0.04290290630677873 1104 7 0.04332688425055409 1104 8 -0.04692235413905621 1104 9 0.0883623932208053 1104 10-11 0.13091420073039473 1104 12-13 0.04484673017091012 1104 14-15 0.11072209123831556 1104 16-17 0.15125420482105056 1104 18-19 0.15838726535135805 1104 20-21 0.23682621512797652 1104 22-23 0.23023315683744983 1104 24-25 0.07779908397525759 1104 26-27 0.07460966012816783 1104 28-29 0.1422585307904285 1104 30-31 0.1350586286630815 1104 32-33 0.1918280383171762 1104 34-35 0.27310346402066443 1104 36-37 0.2965267086494592 1104 38-39 0.23432105487029986 1104 40-41 0.28474300346059067 1104 42-43 0.35590669133132025 1104 44-45 0.3576403850264498 1104 46-47 0.35705123324018473 1104 48-49 0.19868678264777628 1104 50-51 0.23589381589072644 1104 52-53 0.40968225520089874 1104 54-55 0.5661601967466154 1104 56-57 0.5953797752864958 1104 58-59 0.430938100876908 1104 60-61 0.40345297176173034 1104 62-63 0.44180703154115974 1104 64-65 0.6459170101802556 1104 66-67 0.4500615072453584 1104 68-69 0.5273533074165044 1104 70-71 0.6065359913448454 1104 72-73 0.7500344762583389 1104 74-75 0.7527139940417946 1104 76-77 0.6409947064362456 1104 78-79 0.7275576249539313 1104 80-81 0.8703981274806836 1104 82-83 0.9692227753042069 1104 84-85 0.9906502404331547 1104 86-87 1.1158235578285307 1104 88-89 1.0841640603665113 1104 90-91 0.9480186477196284 1104 92-93 1.2151501827069602 1104 94-95 1.3781735454813884 1104 96-97 1.6023665822874733 1104 98-99 1.8178688456264496 1104 100-101 2.0534259626760836 1105 1 0.1363186077553138 1105 2 0.15456178300593848 1105 3 0.19263213335985085 1105 4 0.10450372833444987 1105 5 0.0710118780059048 1105 6 0.10193578357095134 1105 7 0.07196493287747785 1105 8 0.11793751030742783 1105 9 0.05019344620833266 1105 10-11 0.07164621907988789 1105 12-13 0.05692075770579663 1105 14-15 0.10142229546232784 1105 16-17 0.049224903811399656 1105 18-19 0.13777111177917334 1105 20-21 0.0960130274059594 1105 22-23 0.11945397087888665 1105 24-25 0.09538822688804771 1105 26-27 0.18849222398652188 1105 28-29 0.049052480637492124 1105 30-31 0.06619138494019694 1105 32-33 9.206087517839023E-4 1105 34-35 0.07720948709491182 1105 36-37 0.029301727424467572 1105 38-39 0.03207492425497804 1105 40-41 0.05176456103215088 1105 42-43 0.1437965144127631 1105 44-45 0.11476188030368917 1105 46-47 0.08854201514734683 1105 48-49 0.20473613522698741 1105 50-51 0.26719037823107783 1105 52-53 0.3348070105922929 1105 54-55 0.5122882776145232 1105 56-57 0.36254039460147425 1105 58-59 0.5131175885694503 1105 60-61 0.4392782115015095 1105 62-63 0.5445686577031665 1105 64-65 0.5761980549641379 1105 66-67 0.5034785577491121 1105 68-69 0.6088308167639838 1105 70-71 0.5686264928608544 1105 72-73 0.5380610267134109 1105 74-75 0.3589394914959989 1105 76-77 0.5098518123245945 1105 78-79 0.6908643331323603 1105 80-81 0.7151318559227775 1105 82-83 0.7084880090679988 1105 84-85 0.6937617552790769 1105 86-87 0.804089147750453 1105 88-89 0.8181402791409766 1105 90-91 1.103401971248605 1105 92-93 1.388380096788815 1105 94-95 1.3772684769643 1105 96-97 1.3860231924890698 1105 98-99 1.3277945859885065 1105 100-101 1.9338465360773398 1106 1 -0.004815512937128119 1106 2 0.054823752067456155 1106 3 0.09198498888484608 1106 4 0.004162110722162993 1106 5 0.05886111823340201 1106 6 -0.018419399185134466 1106 7 0.029898675239309114 1106 8 -0.014865247523644598 1106 9 0.011604345614706801 1106 10-11 0.08664783694106859 1106 12-13 0.01416939808015627 1106 14-15 0.028994072496168144 1106 16-17 -0.018367780967018632 1106 18-19 -0.07616987362273875 1106 20-21 0.02361933955361195 1106 22-23 -0.05271446091582277 1106 24-25 0.0781838287946286 1106 26-27 -0.010650309584100626 1106 28-29 -0.0370678260094266 1106 30-31 0.04147762191951898 1106 32-33 -0.004514782583953547 1106 34-35 -0.17077783136239333 1106 36-37 0.03720902397842707 1106 38-39 0.023789919339172627 1106 40-41 -0.1627252526649059 1106 42-43 -0.07954548899719072 1106 44-45 0.0847035750755083 1106 46-47 -0.21910300121794535 1106 48-49 -0.0646022839981768 1106 50-51 0.0676723141886626 1106 52-53 -0.052551270990214505 1106 54-55 -0.12156453435266457 1106 56-57 -0.021311483984270296 1106 58-59 0.06098605645490096 1106 60-61 0.07419165592797583 1106 62-63 0.11816609650445997 1106 64-65 0.06206852650844752 1106 66-67 0.16029753152731985 1106 68-69 0.009855669600838723 1106 70-71 -0.0030606252305638293 1106 72-73 0.08641049717499882 1106 74-75 0.357768097770812 1106 76-77 0.26600279610864774 1106 78-79 0.4651736308561567 1106 80-81 0.5034424977158665 1106 82-83 0.43717019890534203 1106 84-85 0.42404376092914475 1106 86-87 0.7031716337808476 1106 88-89 0.6089187814993231 1106 90-91 0.5247207147927178 1106 92-93 0.47209694619082754 1106 94-95 0.8523574593296139 1106 96-97 0.8184653539516127 1106 98-99 1.1233675577506048 1106 100-101 1.9482980811347765 1107 1 0.1024347555913323 1107 2 0.05488572017453919 1107 3 0.09316651412652277 1107 4 0.08457605767745946 1107 5 0.06099695232412472 1107 6 0.04221845919708045 1107 7 0.02040929244165568 1107 8 -0.007627372616596517 1107 9 0.03033936998882325 1107 10-11 0.02491314306352166 1107 12-13 0.09216245765215803 1107 14-15 0.14775181850954766 1107 16-17 0.10685117301134284 1107 18-19 0.04927836235180649 1107 20-21 -0.008868473385327036 1107 22-23 0.01251729980466365 1107 24-25 0.09148011897062958 1107 26-27 -0.05103699057228539 1107 28-29 -0.04161008825845158 1107 30-31 0.04076085748095437 1107 32-33 0.13695840588171393 1107 34-35 0.16007195794603746 1107 36-37 0.17697808949936444 1107 38-39 0.1214495904950823 1107 40-41 0.06328482748050845 1107 42-43 0.1410981530750277 1107 44-45 0.16346710477888848 1107 46-47 0.2931832088126285 1107 48-49 0.39013207938280203 1107 50-51 0.32367289255766707 1107 52-53 0.24945236447206298 1107 54-55 0.33173010334046893 1107 56-57 0.3126676947317506 1107 58-59 0.34602902100915145 1107 60-61 0.3420219459387184 1107 62-63 0.4519655677099479 1107 64-65 0.3658237937975528 1107 66-67 0.47221399851897417 1107 68-69 0.45808751294546823 1107 70-71 0.481800566209575 1107 72-73 0.44123781271659723 1107 74-75 0.5616121860133951 1107 76-77 0.4876007470299051 1107 78-79 0.48253916006378716 1107 80-81 0.4933933363509162 1107 82-83 0.7908800229159176 1107 84-85 0.7681030850636574 1107 86-87 0.8451013206353437 1107 88-89 0.9612343231005767 1107 90-91 0.8533603082819354 1107 92-93 1.0855894687059084 1107 94-95 1.0983026795229556 1107 96-97 1.1880410789784683 1107 98-99 1.2392747708382679 1107 100-101 1.9790982959575452 1108 1 -0.010141773655050201 1108 2 0.02850546201569415 1108 3 0.07775740568472855 1108 4 -0.016538704230946166 1108 5 0.025903587463055544 1108 6 0.02225447424560656 1108 7 -0.020478058911585606 1108 8 -0.01058255158275756 1108 9 -0.08575124894333186 1108 10-11 0.04149088300995629 1108 12-13 0.024070687025187 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-1.5720477309311462 2316 84-85 -1.6016543811349777 2316 86-87 -1.7365710118896338 2316 88-89 -1.7269319227792153 2316 90-91 -1.9537312885304168 2316 92-93 -2.3721682817084186 2316 94-95 -2.3305954125407844 2316 96-97 -2.314990708885894 2316 98-99 -2.8794023527512635 2316 100-101 -3.61602445200219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 11.0 5 23.0 6 87.0 7 197.0 8 497.0 9 963.0 10 1681.0 11 2782.0 12 4300.0 13 6322.0 14 8333.0 15 11537.0 16 15652.0 17 23382.0 18 23053.0 19 16268.0 20 13494.0 21 12923.0 22 12895.0 23 13719.0 24 15362.0 25 17266.0 26 19011.0 27 21571.0 28 26116.0 29 31764.0 30 38610.0 31 47907.0 32 60739.0 33 80816.0 34 115862.0 35 187508.0 36 291762.0 37 412482.0 38 264084.0 39 18051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.383166450013704 18.946108997170118 15.195719627217482 21.475004925598697 2 15.771756486092132 21.304687401099375 38.446618293540084 24.476937819268414 3 19.712207646393516 25.078112315520368 31.041121449033675 24.168558589052445 4 12.629620294127037 17.3345301529192 37.08618091641452 32.94966863653925 5 12.526196566708785 38.428657282865686 34.21541282707184 14.82973332335369 6 30.77419660193106 36.96594226188642 16.43597911319118 15.82388202299134 7 27.020348501242132 35.05551342349472 20.347675722797213 17.576462352465935 8 27.13128805994381 32.268228184927644 19.7679186146662 20.832565140462346 9 26.52437648524687 16.729314411696397 20.705675940734316 36.040633162322415 10-11 24.907266661456724 26.365381461477416 26.604671999154668 22.122679877911196 12-13 27.140956875970673 24.452543094860975 26.990492218654754 21.416007810513594 14-15 23.12037502793007 25.782950053878896 26.819499647228074 24.277175270962964 16-17 20.84571727330889 29.06489271685367 27.940314935712664 22.149075074124777 18-19 20.982334986940284 26.883532430251872 31.154741948159586 20.979390634648258 20-21 21.78724385510925 25.318898118806544 31.46549494286856 21.42836308321565 22-23 21.469584015304026 25.54032992155365 31.967961044292963 21.02212501884936 24-25 21.937740505270202 25.92770642726944 31.419344328698607 20.715208738761746 26-27 20.819771394285684 25.852399864587035 32.030396358161795 21.29743238296549 28-29 20.80815263124005 27.32333424874507 31.17037500144466 20.69813811857022 30-31 20.83012755952833 26.06505987229738 30.832967352289497 22.271845215884788 32-33 20.453936924127277 26.47602148741545 31.11440169226743 21.955639896189837 34-35 22.205996729336945 26.22235664978861 30.55791170750959 21.01373491336486 36-37 22.01401240195818 25.411465357983875 31.28631031974684 21.288211920311102 38-39 21.040450495826143 26.221124973509152 30.922459561889504 21.8159649687752 40-41 21.446428310937886 26.26937898351698 31.054594876822506 21.229597828722635 42-43 21.17642622847949 26.383816343617017 30.036159029271253 22.403598398632244 44-45 21.152420929920876 26.06985098138072 30.808840668771587 21.968887419926812 46-47 22.46246340451961 26.903013136810895 29.610672795472297 21.023850663197198 48-49 21.508377812932796 26.930738404920334 29.290714002481522 22.27016977966535 50-51 21.03049028143605 27.244201827369597 30.09111532064822 21.634192570546134 52-53 22.156608722547354 27.08366583417628 29.136154554830444 21.623570888445922 54-55 20.959027824404334 27.03275880856647 29.574697942475613 22.433515424553587 56-57 21.695528657078167 27.01767932206151 28.843232777740475 22.443559243119847 58-59 21.970261491504306 26.56521217497928 28.74959136792957 22.71493496558685 60-61 20.835475097383764 27.400995466247817 29.790268557949805 21.973260878418614 62-63 20.796648171364605 27.535940360080524 29.777555560202924 21.889855908351944 64-65 20.36118672256717 28.125526268575562 28.968601647296317 22.54468536156096 66-67 20.57351187347726 27.39421903729872 28.747028810153697 23.28524027907033 68-69 21.228160499979502 26.947286911723474 29.050521605396046 22.774030982900985 70-71 21.551585602502534 27.204356760838245 28.179019921432573 23.065037715226648 72-73 21.130653293987248 26.99833465232978 28.941276957333862 22.929735096349113 74-75 20.86588172619395 28.44249817560227 28.051642288163485 22.639977810040296 76-77 21.098513074575216 27.10471657719872 28.606143624954463 23.19062672327161 78-79 21.142568289711058 27.572153141541346 28.54040476863995 22.744873800107648 80-81 21.988602858118544 27.42507090536806 28.02445413116812 22.561872105345277 82-83 21.42729186198402 27.229672296117574 28.6784168486724 22.664618993226 84-85 20.99197947344002 27.7774781324661 28.44414002926862 22.78640236482526 86-87 21.530454805744654 27.931537672634192 27.668690942382025 22.869316579239126 88-89 20.972798022274723 27.95581487883846 27.035222727990682 24.036164370896135 90-91 21.005869124683677 27.353015961773636 27.643747874203477 23.997367039339206 92-93 21.641664751909367 27.228123955196054 27.960226248639604 23.16998504425497 94-95 21.038780210617848 26.496737817378314 28.429777081894404 24.03470489010944 96-97 20.746580137278812 26.199098309771102 28.974448441194212 24.079873111755877 98-99 20.755979665751806 27.095105042120863 27.58934482752927 24.559570464598057 100-101 20.653713728434088 25.937815769834472 28.38304380656339 25.025426695168047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3304.0 1 2176.0 2 1358.5 3 1641.5 4 1320.0 5 1321.0 6 2402.0 7 5018.5 8 7919.5 9 8679.5 10 6562.5 11 4404.5 12 4036.0 13 3967.5 14 3995.5 15 4076.0 16 4097.0 17 4214.5 18 4452.5 19 4749.5 20 5030.0 21 5547.5 22 6338.5 23 7317.5 24 8595.5 25 10221.5 26 12546.5 27 15125.5 28 17193.0 29 19287.0 30 21846.0 31 26283.5 32 34718.5 33 36904.5 34 32610.5 35 32529.0 36 35974.5 37 39596.0 38 41849.5 39 43078.5 40 44597.5 41 47450.0 42 49066.0 43 51148.5 44 53532.0 45 58379.0 46 61062.5 47 68792.0 48 84570.0 49 80519.0 50 65820.5 51 59566.5 52 56580.5 53 57687.0 54 60115.0 55 57134.5 56 52071.5 57 47765.5 58 45927.0 59 41578.5 60 32403.0 61 27023.5 62 24176.5 63 17585.5 64 10806.0 65 7783.5 66 5809.0 67 4709.0 68 3785.0 69 2728.0 70 1922.0 71 1261.0 72 908.0 73 686.5 74 483.5 75 346.5 76 246.5 77 152.5 78 93.0 79 60.0 80 28.5 81 13.0 82 9.5 83 4.5 84 5.0 85 3.5 86 1.5 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010401543611085735 3 0.00566856609493032 4 0.0 5 3.302077336852614E-4 6 3.302077336852614E-4 7 0.0 8 1.651038668426307E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.751731114043845E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001651038668426307 26-27 0.020527914110767084 28-29 2.751731114043845E-4 30-31 2.201384891235076E-4 32-33 0.003714837003959191 34-35 0.015464728860926409 36-37 0.008117606786429343 38-39 0.020527914110767084 40-41 0.022646747068580844 42-43 0.018766806197779023 44-45 0.010153887810821789 46-47 0.002449040691499022 48-49 3.5772504482569983E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.751731114043845E-4 68-69 1.9262117798306914E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.9262117798306914E-4 82-83 9.080712676344689E-4 84-85 0.004127596671065768 86-87 5.228289116683305E-4 88-89 5.50346222808769E-5 90-91 0.003962492804223137 92-93 5.778635339492074E-4 94-95 1.651038668426307E-4 96-97 3.302077336852614E-4 98-99 2.751731114043845E-4 100-101 0.002751731114043845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1817038.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.50507439032621 #Duplication Level Percentage of deduplicated Percentage of total 1 67.05788913928221 25.15011120627027 2 15.444100674415163 11.584642893712555 3 6.576146076495383 7.399165434018334 4 3.392441293415257 5.089350522974147 5 1.994460820738998 3.7401200725203587 6 1.228698828044992 2.7649464569480435 7 0.8401380821713517 2.2056608888987776 8 0.6124984782125081 1.8377440791457367 9 0.41839433933844206 1.412271973924169 >10 2.1637323565652977 14.563762397128825 >50 0.16480319506776497 4.26490518807683 >100 0.08798086823310793 6.510140129968289 >500 0.0067789685740093746 1.7498738078324514 >1k 0.011052666153276153 8.210589194602854 >5k 4.421066461310461E-4 1.1862220992956436 >10k+ 4.421066461310461E-4 2.3304936546827326 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16810 0.9251320005415407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13599 0.748415828397645 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11756 0.6469870195339888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9221 0.5074742520519658 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7036 0.3872236023682499 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5205 0.28645520897196425 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4135 0.227568163131426 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3766 0.20726038750978243 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3694 0.20329789470555928 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3541 0.1948775974965851 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3382 0.18612709255392568 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3344 0.18403577690725234 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3332 0.18337536143988184 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3185 0.17528527196459293 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3110 0.17115767529352716 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3094 0.17027712133703313 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2963 0.16306758581823827 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2961 0.16295751657367652 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2936 0.16158165101665456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2921 0.16075613168244143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2778 0.15288618069627605 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2748 0.1512351420278497 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2735 0.1505196919381983 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2721 0.14974920722626606 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2690 0.14804313393555887 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2674 0.14716257997906482 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2670 0.14694244148994132 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2663 0.14655719913397516 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2362 0.12999177782743124 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2239 0.12322251928688338 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2225 0.1224520345749511 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2219 0.12212182684126585 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 2162 0.11898485337125586 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 1964 0.10808799815964223 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 1940 0.10676716722490119 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 1915 0.10539130166787926 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1913 0.1052812324233175 RNA PCR Primer, Index 31 (95% over 22bp) GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1878 0.10335502064348681 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1848 0.1017039819750605 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 5.50346222808769E-5 4 0.0 0.0 0.0 0.0 5.50346222808769E-5 5 0.0 0.0 0.0 0.0 5.50346222808769E-5 6 0.0 0.0 0.0 0.0 5.50346222808769E-5 7 0.0 0.0 0.0 0.0 5.50346222808769E-5 8 0.0 0.0 0.0 0.0 5.50346222808769E-5 9 0.0 0.0 0.0 0.0 5.50346222808769E-5 10-11 0.0 0.0 0.0 0.0 1.100692445617538E-4 12-13 0.0 0.0 0.0 2.751731114043845E-5 5.778635339492074E-4 14-15 0.0 0.0 0.0 1.100692445617538E-4 6.604154673705228E-4 16-17 0.0 0.0 0.0 2.4765580026394604E-4 7.704847119322766E-4 18-19 0.0 0.0 0.0 4.1275966710657675E-4 7.980020230727151E-4 20-21 0.0 0.0 0.0 6.879327785109612E-4 9.080712676344689E-4 22-23 0.0 0.0 0.0 0.0013758655570219225 9.355885787749073E-4 24-25 0.0 0.0 0.0 0.0037698716262400678 9.355885787749073E-4 26-27 0.0 0.0 0.0 0.007732364430463204 9.355885787749073E-4 28-29 0.0 0.0 0.0 0.019152048553745162 9.355885787749073E-4 30-31 0.0 0.0 0.0 0.05277820276736095 9.631058899153457E-4 32-33 0.0 0.0 0.0 0.11488477401133053 0.0011282097567579764 34-35 0.0 0.0 0.0 0.20007836930212797 0.0011557270678984148 36-37 0.0 0.0 0.0 0.324346546412348 0.0011557270678984148 38-39 0.0 0.0 0.0 0.4990264375318513 0.0011832443790388532 40-41 0.0 0.0 0.0 0.681273589214975 0.0012107616901792918 42-43 0.0 0.0 0.0 0.8692993762375911 0.0012107616901792918 44-45 0.0 0.0 0.0 1.0976105067698088 0.0012657963124601689 46-47 0.0 0.0 0.0 1.3506597000172809 0.0013208309347410457 48-49 0.0 0.0 0.0 1.588959614493478 0.0014309001793027993 50-51 0.0 0.0 0.0 1.848860618214919 0.0014859348015836763 52-53 0.0 0.0 0.0 2.086665221090588 0.0015960040461454302 54-55 0.0 0.0 0.0 2.3450527726993053 0.0017060732907071838 56-57 0.0 0.0 0.0 2.644193462107012 0.0017335906018476222 58-59 0.0 0.0 0.0 2.9398669703110225 0.0018161425352689377 60-61 0.0 0.0 0.0 3.2513629324207862 0.0018161425352689377 62-63 0.0 0.0 0.0 3.5495680332497175 0.0018161425352689377 64-65 0.0 0.0 0.0 3.8507725209929564 0.0019812464021115683 66-67 0.0 0.0 0.0 4.200214855165385 0.0019812464021115683 68-69 0.0 0.0 0.0 4.583503482040552 0.002036281024392445 70-71 0.0 0.0 0.0 4.970892188275644 0.002063798335532884 72-73 0.0 0.0 0.0 5.359711794690039 0.0021188329578137608 74-75 0.0 0.0 0.0 5.759703429427454 0.002146350268954199 76-77 0.0 0.0 0.0 6.16181389712268 0.0021738675800946376 78-79 0.0 0.0 0.0 6.638854003053321 0.0022289022023755144 80-81 0.0 0.0 0.0 7.121782813568016 0.002283936824656391 82-83 0.0 0.0 0.0 7.596236292251455 0.0024215233803585837 84-85 0.0 0.0 0.0 8.092758654469527 0.0024215233803585837 86-87 0.0 0.0 0.0 8.59123474577857 0.002504075313779899 88-89 0.0 0.0 0.0 9.108229987485126 0.002586627247201214 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 1255 0.0 30.283348 2 TAAGGTG 1430 0.0 29.892107 5 TAAGGTA 635 0.0 27.674393 4 GGTAAGG 1550 0.0 25.742188 3 GTAAGGT 1630 0.0 25.641602 4 GTGTAAG 605 0.0 25.127672 1 AAGGTAA 1455 0.0 24.814655 1 GAGTACT 11920 0.0 23.747618 12-13 GTACATG 9140 0.0 22.817917 1 GTACTTT 12360 0.0 22.671677 14-15 TACATGG 9045 0.0 22.427298 2 AAGGTGT 1910 0.0 22.131289 6 AGTACTT 12120 0.0 22.02337 12-13 ACATGGG 8880 0.0 21.717556 3 GGTGTGC 1920 0.0 21.52128 8 AGGTGTG 1945 0.0 21.48885 7 AGAGTAC 17125 0.0 21.050444 10-11 GTAAGCG 215 0.0 20.988 94-95 ACTTTTT 13720 0.0 20.25125 16-17 TACTTTT 13040 0.0 20.123558 14-15 >>END_MODULE