##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938037_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1837729 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57260782193675 34.0 31.0 34.0 31.0 34.0 2 32.77330281015318 34.0 31.0 34.0 31.0 34.0 3 32.818231088479315 34.0 31.0 34.0 31.0 34.0 4 36.26287880313147 37.0 35.0 37.0 35.0 37.0 5 36.23781580418005 37.0 37.0 37.0 35.0 37.0 6 36.295032619064074 37.0 37.0 37.0 35.0 37.0 7 36.26170779260707 37.0 37.0 37.0 35.0 37.0 8 36.25848533706547 37.0 37.0 37.0 35.0 37.0 9 37.97224400333237 39.0 38.0 39.0 35.0 39.0 10-11 37.964433003995694 39.0 38.0 39.0 35.0 39.0 12-13 37.9284062013496 39.0 38.0 39.0 35.0 39.0 14-15 39.22147063032689 41.0 39.0 41.0 36.0 41.0 16-17 39.20524952264452 40.0 39.0 41.0 36.0 41.0 18-19 39.27329628035472 40.5 39.0 41.0 36.0 41.0 20-21 39.22568643145969 40.5 39.0 41.0 36.0 41.0 22-23 39.09207396738039 40.0 39.0 41.0 35.5 41.0 24-25 39.02255963746559 40.0 38.5 41.0 35.0 41.0 26-27 38.94623744850302 40.0 38.0 41.0 35.0 41.0 28-29 38.882125710591716 40.0 38.0 41.0 35.0 41.0 30-31 38.63612589233777 40.0 38.0 41.0 35.0 41.0 32-33 38.37599286946008 40.0 38.0 41.0 34.5 41.0 34-35 38.13737335591918 40.0 37.5 41.0 34.0 41.0 36-37 37.95454498459783 40.0 37.0 41.0 33.5 41.0 38-39 37.869454364598916 40.0 37.0 41.0 33.0 41.0 40-41 37.78797227447572 40.0 37.0 41.0 33.0 41.0 42-43 37.576717513844535 40.0 37.0 41.0 33.0 41.0 44-45 37.30827804317176 40.0 37.0 41.0 33.0 41.0 46-47 36.88693789998416 40.0 36.0 41.0 31.5 41.0 48-49 36.438339657261764 40.0 35.5 41.0 30.5 41.0 50-51 36.081531335686606 39.5 35.0 41.0 30.0 41.0 52-53 35.676247966920045 39.0 35.0 41.0 28.0 41.0 54-55 35.431621855017795 39.0 35.0 41.0 28.0 41.0 56-57 35.14036101079104 38.5 35.0 40.5 27.0 41.0 58-59 34.78423287655579 38.0 35.0 40.0 26.0 41.0 60-61 34.382326773969396 37.5 34.0 40.0 26.0 41.0 62-63 34.022511208126986 37.0 34.0 40.0 25.0 41.0 64-65 33.61440587812458 36.5 34.0 39.5 23.5 41.0 66-67 33.165304840920506 36.0 33.5 39.0 22.0 41.0 68-69 32.68813220012309 35.0 33.0 39.0 20.5 40.0 70-71 32.14328472805294 35.0 32.5 37.5 18.5 40.0 72-73 31.749663851416614 35.0 32.0 37.0 16.5 39.0 74-75 31.354002956910406 35.0 32.0 36.5 12.0 39.0 76-77 30.04799592322916 33.5 30.0 35.0 9.0 37.5 78-79 30.724145126947445 35.0 31.5 36.0 7.5 37.5 80-81 30.57087252799515 35.0 31.0 35.0 6.0 37.0 82-83 30.235935766372517 35.0 31.0 35.0 2.0 37.0 84-85 29.911724470800646 34.0 31.0 35.0 2.0 36.0 86-87 29.588117453661557 34.0 31.0 35.0 2.0 36.0 88-89 29.1733443832034 34.0 30.0 35.0 2.0 35.5 90-91 28.899893020135178 34.0 30.0 35.0 2.0 35.0 92-93 28.583292748822053 34.0 29.0 35.0 2.0 35.0 94-95 28.257113263163394 34.0 29.0 35.0 2.0 35.0 96-97 27.784272871571382 34.0 29.0 35.0 2.0 35.0 98-99 27.237113306695385 34.0 27.0 35.0 2.0 35.0 100-101 24.948666261456395 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12081512387613458 1101 2 0.141586998775729 1101 3 0.1733142596511854 1101 4 0.036422709355861116 1101 5 0.06427069036576682 1101 6 0.06259952028761973 1101 7 0.06681154711325377 1101 8 0.08301265861766893 1101 9 0.09639713768793712 1101 10-11 0.10400358605345161 1101 12-13 0.1212211395825733 1101 14-15 0.18130283934657854 1101 16-17 0.10609530267990408 1101 18-19 0.22828488839590477 1101 20-21 0.1888071197444745 1101 22-23 0.2614777930899521 1101 24-25 0.15219898124607312 1101 26-27 0.17086079331114234 1101 28-29 0.21535020249632453 1101 30-31 0.20030642918759156 1101 32-33 0.20586629117626387 1101 34-35 0.2013656109673363 1101 36-37 0.311206015262087 1101 38-39 0.3323095364879478 1101 40-41 0.45179105365321703 1101 42-43 0.5621256473951135 1101 44-45 0.44324980531941094 1101 46-47 0.40294263993863666 1101 48-49 0.5006479361807905 1101 50-51 0.29520616963564095 1101 52-53 0.3343607997654061 1101 54-55 0.3955538838756141 1101 56-57 0.5423146115928219 1101 58-59 0.40175666538846855 1101 60-61 0.6016382639668549 1101 62-63 0.6716194338487682 1101 64-65 0.5879605712303757 1101 66-67 0.5224211857337977 1101 68-69 0.5333678133067963 1101 70-71 0.5945074454334147 1101 72-73 0.3899569526496087 1101 74-75 0.4153020908710161 1101 76-77 0.23042082449253698 1101 78-79 0.34121464694573334 1101 80-81 0.5186667342682796 1101 82-83 0.5131895618730695 1101 84-85 0.571320039504112 1101 86-87 0.6438890328202227 1101 88-89 0.5655164948522682 1101 90-91 0.5704928721891491 1101 92-93 0.7519540422417315 1101 94-95 0.530520918050776 1101 96-97 0.6916233499441269 1101 98-99 0.8041319437640944 1101 100-101 0.9925910456384059 1102 1 0.18008615525731386 1102 2 0.18831887128735048 1102 3 0.1679428900372173 1102 4 0.12103998210960754 1102 5 0.08628910387510302 1102 6 0.10175880588006692 1102 7 0.1379760580486149 1102 8 0.1633256799558751 1102 9 0.23490616762964578 1102 10-11 0.19723289789742182 1102 12-13 0.23036174540616372 1102 14-15 0.27678308413891983 1102 16-17 0.2478691318661106 1102 18-19 0.25767161785544346 1102 20-21 0.17190962549609168 1102 22-23 0.19619678529770113 1102 24-25 0.1882844255874332 1102 26-27 0.10828273559106805 1102 28-29 0.10169088154128758 1102 30-31 0.19109655428795236 1102 32-33 0.16974964454839636 1102 34-35 0.21217886736275915 1102 36-37 0.08977628489144962 1102 38-39 0.08666558369908728 1102 40-41 0.24282622071026339 1102 42-43 0.1105765578778275 1102 44-45 0.02731313348009934 1102 46-47 0.018605771732936205 1102 48-49 -0.02033081714225915 1102 50-51 -0.07682763700170625 1102 52-53 -0.030972419852929534 1102 54-55 -0.09827409583873958 1102 56-57 -0.01745378423522226 1102 58-59 -0.011048336962105054 1102 60-61 0.009786637079642446 1102 62-63 -0.03853836651483533 1102 64-65 0.12648832254399878 1102 66-67 0.05399124318760329 1102 68-69 0.06755678267447252 1102 70-71 0.15500784690283353 1102 72-73 0.05326639196531957 1102 74-75 0.031182182564133853 1102 76-77 0.11886578573929896 1102 78-79 0.09992320510887609 1102 80-81 0.265662994154205 1102 82-83 0.28589338560927047 1102 84-85 0.30889154474401437 1102 86-87 0.22670468555125112 1102 88-89 0.052017969680342446 1102 90-91 0.06335254094567588 1102 92-93 0.27477581062091616 1102 94-95 0.0975147233568272 1102 96-97 0.02965332078038685 1102 98-99 -0.1106999591177491 1102 100-101 0.17861886975735786 1103 1 0.15428997691336832 1103 2 0.14090654871579034 1103 3 0.11122760497798367 1103 4 0.054714218013138805 1103 5 0.08274835988850526 1103 6 0.08894248637584923 1103 7 0.07663394001860269 1103 8 0.011395289808923792 1103 9 0.05658960555512493 1103 10-11 0.08494436443054809 1103 12-13 0.08246858588880457 1103 14-15 0.23088682598150712 1103 16-17 0.16130283192401862 1103 18-19 0.1693846337085816 1103 20-21 0.19104646644822054 1103 22-23 0.166400544354957 1103 24-25 0.16969649776383733 1103 26-27 0.17789297988284858 1103 28-29 0.10260091143220507 1103 30-31 0.06411491138274528 1103 32-33 0.18408867958807207 1103 34-35 0.2484306083915726 1103 36-37 0.1876072368625259 1103 38-39 0.2776157550632732 1103 40-41 0.26932311424537403 1103 42-43 0.24210248160120784 1103 44-45 0.3460959000335677 1103 46-47 0.3015178673838861 1103 48-49 0.19790342752511236 1103 50-51 0.1935236204514652 1103 52-53 0.3356350387927449 1103 54-55 0.42098129156951103 1103 56-57 0.34683125934918024 1103 58-59 0.16440276555906053 1103 60-61 0.2556484659032492 1103 62-63 0.4404969079025989 1103 64-65 0.34599136641009665 1103 66-67 0.29131811841256905 1103 68-69 0.2743452300680218 1103 70-71 0.3367286093560793 1103 72-73 0.25762365890220096 1103 74-75 0.18862596102702156 1103 76-77 0.15551033416728544 1103 78-79 0.23554348085653842 1103 80-81 0.26713993432541017 1103 82-83 0.371755449178341 1103 84-85 0.4914480010265869 1103 86-87 0.4981068201699479 1103 88-89 0.4140377195981806 1103 90-91 0.48079021602666216 1103 92-93 0.5512441434586783 1103 94-95 0.41541171705000224 1103 96-97 0.5686328040273843 1103 98-99 0.603165672582282 1103 100-101 0.7807718768466714 1104 1 0.17690902453241364 1104 2 0.17018875299799419 1104 3 0.18721040596078353 1104 4 0.10045687625579802 1104 5 0.14492953456967683 1104 6 0.07719859963196285 1104 7 0.10151108989575164 1104 8 0.14304967146330227 1104 9 0.07167823314374999 1104 10-11 0.1511057368419202 1104 12-13 0.15150596742618205 1104 14-15 0.26558540443009093 1104 16-17 0.25529633841752286 1104 18-19 0.23113802671198158 1104 20-21 0.30514059304234564 1104 22-23 0.27798136218578406 1104 24-25 0.21382089813823768 1104 26-27 0.29791535615522236 1104 28-29 0.2677414578227584 1104 30-31 0.3635013920192236 1104 32-33 0.3763674031167952 1104 34-35 0.48197527318623656 1104 36-37 0.48467126517655146 1104 38-39 0.4703515102990323 1104 40-41 0.5851298300520895 1104 42-43 0.7421412378899603 1104 44-45 0.5589081440958168 1104 46-47 0.5002652348812546 1104 48-49 0.6708164379381216 1104 50-51 0.7608827815606176 1104 52-53 0.5562716656793683 1104 54-55 0.630761770099987 1104 56-57 0.8333689333868577 1104 58-59 0.8800003436566328 1104 60-61 0.6999235039282894 1104 62-63 0.8434262983319911 1104 64-65 0.8545630874818215 1104 66-67 0.9585427293871831 1104 68-69 0.8154393274121219 1104 70-71 0.902334962462433 1104 72-73 0.8620338320623837 1104 74-75 0.8565006539017084 1104 76-77 0.8357921581991086 1104 78-79 0.8755582521213121 1104 80-81 0.9474722146576902 1104 82-83 0.8827958727187664 1104 84-85 0.9909339317625161 1104 86-87 0.966921170234297 1104 88-89 0.8884146939751538 1104 90-91 0.7338958378822831 1104 92-93 0.9431353040998367 1104 94-95 0.9444153148035994 1104 96-97 0.8787547766493624 1104 98-99 1.0371351940518032 1104 100-101 0.9379739652987595 1105 1 0.040190316664215686 1105 2 0.05850375057120516 1105 3 0.04273135726316468 1105 4 0.0011923289872868281 1105 5 0.025252971985409545 1105 6 0.06232101779515631 1105 7 0.03172060291953471 1105 8 -0.03862265771751794 1105 9 -0.044789571418618834 1105 10-11 0.01913781093996647 1105 12-13 0.012521579786636039 1105 14-15 0.07937665989037868 1105 16-17 0.04900488072459552 1105 18-19 0.08801764435744985 1105 20-21 0.1023909031324095 1105 22-23 0.11095033969751 1105 24-25 0.09541739007589456 1105 26-27 0.05103075010442382 1105 28-29 -0.008197238720413225 1105 30-31 0.23308391642196113 1105 32-33 0.21247637954994758 1105 34-35 0.22146827593660134 1105 36-37 0.20324801920772728 1105 38-39 0.23254276845575106 1105 40-41 0.2401789051747798 1105 42-43 0.24686101085010392 1105 44-45 0.21369532793877255 1105 46-47 0.1672355875749858 1105 48-49 0.21125555242876004 1105 50-51 0.09959718861448863 1105 52-53 -0.021218829386150162 1105 54-55 -0.15552247462847646 1105 56-57 -0.08888946600914238 1105 58-59 0.09381890052446096 1105 60-61 0.06668083823234383 1105 62-63 0.11958643962520199 1105 64-65 -0.07159435737607822 1105 66-67 0.03555056253176048 1105 68-69 0.11027666495188981 1105 70-71 0.11753679064557332 1105 72-73 0.1695329557209 1105 74-75 0.1473556935393141 1105 76-77 0.11850295202966521 1105 78-79 0.11865638888451002 1105 80-81 0.18010312659211536 1105 82-83 0.29580819542814396 1105 84-85 0.2918315076671725 1105 86-87 0.32701089411932216 1105 88-89 0.2699872550494149 1105 90-91 0.12216802954490547 1105 92-93 0.12830929158837634 1105 94-95 0.07116563837583456 1105 96-97 0.1645762456673161 1105 98-99 0.18504882722874072 1105 100-101 0.204586366204758 1106 1 -0.15022026105010866 1106 2 -0.10259368039594818 1106 3 -0.028713777769155513 1106 4 -0.03551467617258908 1106 5 -0.07793953281377952 1106 6 -0.07616991434805698 1106 7 0.004873015739690345 1106 8 -0.14301378546345944 1106 9 -0.19527864091743652 1106 10-11 -0.037495634808010436 1106 12-13 -0.1487005706400666 1106 14-15 -0.21988627317288234 1106 16-17 -0.2261676113018254 1106 18-19 -0.2518726526566155 1106 20-21 0.02526899581463482 1106 22-23 -0.0064200727102559085 1106 24-25 -0.09933643313959806 1106 26-27 -0.19916494663551276 1106 28-29 -0.10255675415710641 1106 30-31 -0.030623472923984707 1106 32-33 -0.024761968111498334 1106 34-35 0.06758530164698584 1106 36-37 -0.06835680687130008 1106 38-39 -0.21212503593650922 1106 40-41 -0.2036037984297394 1106 42-43 -0.3255441193187707 1106 44-45 -0.35694571128142627 1106 46-47 -0.3556507466192613 1106 48-49 -0.4110276472333041 1106 50-51 -0.2171416670123918 1106 52-53 -0.1950006681019545 1106 54-55 -0.3715702653705293 1106 56-57 -0.2127595484430671 1106 58-59 -0.26775738422698936 1106 60-61 -0.3382769796408951 1106 62-63 -0.27594315116335366 1106 64-65 -0.42736664345870423 1106 66-67 -0.4248148153462594 1106 68-69 -0.5200247738343649 1106 70-71 -0.4848132310706532 1106 72-73 -0.47759166534189745 1106 74-75 -0.4717090859986719 1106 76-77 -0.3815799331388092 1106 78-79 -0.35884647684277127 1106 80-81 -0.6779770962232732 1106 82-83 -0.6219966668759334 1106 84-85 -0.4826866772447822 1106 86-87 -0.32735983353411413 1106 88-89 -0.27045161956741737 1106 90-91 -0.4330552715598337 1106 92-93 -0.44710618402834257 1106 94-95 -0.4964006587911527 1106 96-97 -0.41024336549626383 1106 98-99 -0.2884277621766209 1106 100-101 -0.3688086855036765 1107 1 0.07874480557380537 1107 2 0.09433576382724596 1107 3 0.06927167283513569 1107 4 0.06598313348766283 1107 5 0.04666703642400449 1107 6 0.004416371475905123 1107 7 0.015192294464803524 1107 8 0.05971633812997368 1107 9 0.02637523655570817 1107 10-11 0.0342916513946534 1107 12-13 0.11092408046579294 1107 14-15 0.1643828751317713 1107 16-17 0.06232237313047051 1107 18-19 0.0783352155563577 1107 20-21 0.08471891970011569 1107 22-23 0.07127334408757235 1107 24-25 0.10860375676455902 1107 26-27 0.04954808868748728 1107 28-29 0.10215122640308749 1107 30-31 0.1899820699601804 1107 32-33 0.12327531737283692 1107 34-35 0.13489119812319217 1107 36-37 0.13889263440960775 1107 38-39 0.07674336610677557 1107 40-41 0.11324509484959577 1107 42-43 -0.0430899963482787 1107 44-45 -0.047692323193906816 1107 46-47 -0.207449681303288 1107 48-49 -0.13855346267009594 1107 50-51 -0.11801593524697296 1107 52-53 -0.10972280843426319 1107 54-55 -0.024905972074762417 1107 56-57 0.08109738613867279 1107 58-59 0.09126165503477068 1107 60-61 0.15224419077000562 1107 62-63 0.07400966875181325 1107 64-65 -0.053857399092869684 1107 66-67 0.004317651505566289 1107 68-69 0.038597056235730065 1107 70-71 0.05252224921514426 1107 72-73 -0.09615949978749683 1107 74-75 0.049465347567341666 1107 76-77 0.04199580009200332 1107 78-79 0.08880647196999902 1107 80-81 0.1338280965743195 1107 82-83 0.1536996703748983 1107 84-85 0.29451370479322136 1107 86-87 0.26567228282887 1107 88-89 0.3805894454957013 1107 90-91 0.35216371356838394 1107 92-93 0.3083472533655254 1107 94-95 0.282227768445491 1107 96-97 0.5286082850309057 1107 98-99 0.5519085956242691 1107 100-101 0.7974430722561863 1108 1 0.04759541624391517 1108 2 0.07419353482704594 1108 3 0.07016019675576501 1108 4 0.020886742763075006 1108 5 0.06874652998708797 1108 6 0.06895492559331018 1108 7 0.10412615265725833 1108 8 0.04751271190961148 1108 9 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-1.1620131881725406 2316 100-101 -1.2388728627479857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 8.0 5 39.0 6 67.0 7 205.0 8 490.0 9 953.0 10 1662.0 11 2666.0 12 4247.0 13 6067.0 14 8210.0 15 11054.0 16 14961.0 17 22453.0 18 22217.0 19 15283.0 20 12129.0 21 11630.0 22 11591.0 23 11739.0 24 13333.0 25 14970.0 26 16175.0 27 18504.0 28 21818.0 29 26958.0 30 32677.0 31 40435.0 32 50407.0 33 66407.0 34 95187.0 35 157310.0 36 267482.0 37 438880.0 38 376163.0 39 43347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41073738293296 18.920961686951667 15.126822289902375 21.541478640213 2 15.759609235751117 21.262310026259577 38.52663374015266 24.451446997836644 3 19.497300280039486 25.113381708077608 31.148107475084323 24.241210536798583 4 12.675535946812616 17.406647008345626 37.02401170139885 32.893805343442914 5 12.53438347003285 38.4312921001954 34.16423204944798 14.870092380323758 6 30.742073504853 36.95098678858526 16.519791547067058 15.787148159494679 7 27.035705482146717 35.053155280239906 20.371393170592615 17.539746067020765 8 27.14021645264275 32.247242919634786 19.825185841391765 20.787354786330692 9 26.586912043183947 16.726541584136864 20.729273229871797 35.957273142807395 10-11 24.90544035600461 26.453601156644968 26.59633166805334 22.04462681929708 12-13 27.16850525839229 24.438586973378555 26.984310526742515 21.40859724148664 14-15 23.093502904944092 25.8078585036205 26.84808260630376 24.25055598513165 16-17 20.87459695444344 29.003554119723475 27.96022694345432 22.161621982378772 18-19 20.965496000770518 26.855727912004433 31.127168369220925 21.051607718004124 20-21 21.797637192426087 25.268633188027177 31.499421296611196 21.434308322935536 22-23 21.48456056360867 25.52111872860471 31.965676114378127 21.028644593408494 24-25 21.96378604226957 25.88426204565218 31.460100324656597 20.691851587421652 26-27 20.82811748237133 25.80218880691083 32.082055223071514 21.287638487646323 28-29 20.85194326908008 27.31386255202076 31.161575597694547 20.67261858120462 30-31 20.88466253729467 26.063010378570507 30.85732444772869 22.19500263640613 32-33 20.468036734669447 26.43630500636931 31.128208393122126 21.96744986583912 34-35 22.229406306210695 26.213715613681327 30.525200079460323 21.031678000647656 36-37 22.060609292655776 25.41880129327831 31.205359219940842 21.315230194125068 38-39 21.06588686891446 26.256674174560295 30.911568224547793 21.76587073197745 40-41 21.424151437523367 26.322212915750303 31.017219149998017 21.236416496728314 42-43 21.179382987239464 26.39861295796998 30.038401059654834 22.38360299513572 44-45 21.20207392606088 26.105636799562927 30.778228347356496 21.914060927019698 46-47 22.427850468662808 26.864740901607608 29.61044027994342 21.096968349786163 48-49 21.549096738420083 26.8477561163806 29.306606142690246 22.296541002509073 50-51 21.043799167341867 27.256684745139246 30.100928918246378 21.59858716927251 52-53 22.154953205831763 27.08274723857544 29.10469933270901 21.657600222883787 54-55 20.98130355455021 27.007953838678063 29.594679084892277 22.41606352187945 56-57 21.73652916180786 26.973128246874268 28.88464512449877 22.405697466819102 58-59 21.97957370210733 26.570321304174882 28.790561611641323 22.659543382076468 60-61 20.85087083024755 27.414134510583445 29.806679874997894 21.928314784171114 62-63 20.791095966815565 27.575202872676 29.781349698459348 21.852351462049082 64-65 20.33104445758869 28.121257269162104 28.980197842010437 22.567500431238773 66-67 20.554853299915276 27.46487648614132 28.7501584836502 23.230111730293203 68-69 21.262780081655396 26.935506602464027 29.071601106369133 22.730112209511443 70-71 21.52376656188154 27.246917254938026 28.19213278998155 23.037183393198887 72-73 21.116470382738694 26.944478756116926 29.03031404521559 22.90873681592879 74-75 20.783559491089274 28.452073183804576 28.151457587054455 22.61290973805169 76-77 21.137583397769745 27.117327962936866 28.576465844528766 23.168622794764627 78-79 21.193440382123807 27.61408238102571 28.469703639655247 22.722773597195236 80-81 21.95418911058159 27.50783711853053 27.99300114434718 22.544972626540694 82-83 21.43782975500183 27.25411486357067 28.62695132250257 22.681104058924934 84-85 21.0564579366578 27.765201459022272 28.398340506369756 22.780000097950175 86-87 21.558777236497896 27.956179455820994 27.602807279118586 22.882236028562527 88-89 20.940466386048197 28.01943053005983 27.04025871096706 23.999844372924915 90-91 21.05940196182727 27.42623966419359 27.534743355305242 23.9796150186739 92-93 21.637307887365022 27.26916555478407 27.97373272591532 23.11979383193559 94-95 20.9871803731671 26.62530764873384 28.454929970632232 23.932582007466824 96-97 20.765880270126953 26.271807832332144 28.910418486735118 24.051893410805786 98-99 20.77697527763887 27.17590569665059 27.588724996993573 24.458394028716963 100-101 20.566933427072218 26.202775273176837 28.45427699078591 24.77601430896503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3382.0 1 2178.5 2 1323.5 3 1679.0 4 1366.5 5 1287.0 6 2418.5 7 5163.5 8 8098.5 9 8826.0 10 6684.0 11 4463.0 12 4021.5 13 4120.0 14 4202.0 15 4084.5 16 4056.5 17 4191.5 18 4346.5 19 4703.0 20 5108.5 21 5651.0 22 6406.0 23 7403.5 24 8678.5 25 10349.5 26 12651.0 27 15242.5 28 17281.0 29 19311.0 30 22277.5 31 26754.0 32 35258.0 33 37316.5 34 32826.5 35 33159.5 36 36307.0 37 39777.5 38 42274.0 39 43644.0 40 45355.0 41 48303.0 42 49813.5 43 51632.0 44 54305.5 45 59344.5 46 61746.0 47 69742.0 48 86149.5 49 81457.5 50 66001.5 51 59615.0 52 56696.5 53 57945.5 54 60937.5 55 58365.0 56 53016.0 57 48483.0 58 46378.5 59 41905.0 60 32445.5 61 27139.0 62 24566.5 63 17739.0 64 10808.5 65 7830.0 66 5939.5 67 4868.0 68 3745.5 69 2702.5 70 1940.5 71 1229.5 72 880.5 73 700.5 74 493.5 75 339.5 76 234.5 77 150.5 78 100.0 79 50.0 80 22.0 81 10.0 82 7.5 83 5.0 84 2.5 85 2.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01654215610680356 3 0.007128363322339693 4 0.0 5 0.0 6 0.0 7 0.0 8 3.264899231605966E-4 9 4.897348847408948E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.360374679835819E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0051694237833761125 26-27 0.020732110120697884 28-29 5.441498719343276E-5 30-31 0.0 32-33 5.441498719343276E-4 34-35 0.018174605722606544 36-37 0.003999501558717308 38-39 0.017929738280236095 40-41 0.016215666183642962 42-43 0.008135040585418198 44-45 0.002992824295638802 46-47 2.720749359671638E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.264899231605966E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.441498719343276E-5 84-85 0.003264899231605966 86-87 3.53697416757313E-4 88-89 1.360374679835819E-4 90-91 1.9045245517701468E-4 92-93 2.720749359671638E-4 94-95 0.0 96-97 2.720749359671638E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1837729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.076150652720386 #Duplication Level Percentage of deduplicated Percentage of total 1 66.36830827805483 24.6068139628335 2 15.92814382910896 11.811085204524847 3 6.59036017280501 7.3303555986782065 4 3.4675439158403925 5.142527224744894 5 1.9822715568247933 3.67474994377193 6 1.2549829757246005 2.791796272473877 7 0.8470936461257513 2.1984880148504398 8 0.599626511712843 1.7785474306904459 9 0.4367275665298676 1.4572959345771603 >10 2.2517848233032507 14.89132305137623 >50 0.16736370114787258 4.308704249433841 >100 0.08613141115360072 6.336079790979234 >500 0.007629879154843052 1.8937078405642467 >1k 0.01115136184169369 8.233957021443326 >5k 4.401853358563299E-4 1.1998197766057013 >10k+ 4.401853358563299E-4 2.3447486824521575 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17199 0.9358833647398501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14046 0.7643129101189565 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11856 0.6451440881653389 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9613 0.5230912718904692 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7133 0.3881421036507559 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5309 0.28888916700993456 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4201 0.22859736119961102 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3748 0.20394737200098598 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3727 0.20280465726992392 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3588 0.19524097405003676 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3462 0.18838468566366423 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3322 0.18076658745658364 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3275 0.17820908305849228 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3228 0.17565157866040096 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3137 0.1706998148257986 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3111 0.16928502515876934 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3048 0.16585688096558307 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 3041 0.16547597605522904 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.16019772229746604 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.15954474245114486 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2918 0.1587829326304368 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2812 0.15301494398793294 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2775 0.15100158946177591 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2759 0.150130949666681 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2753 0.14980445974352039 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2689 0.1463219005631407 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2666 0.14507035585769176 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2600 0.14147896670292517 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2326 0.1265692602119246 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2300 0.12515447054489537 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2261 0.12303228604435149 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 2168 0.11797169223536222 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2131 0.11595833770920523 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2113 0.11497886793972344 No Hit TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA 2013 0.10953736922038017 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1981 0.10779608963019031 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 1948 0.10600039505280702 No Hit GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG 1910 0.10393262553945659 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1878 0.10219134594926674 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1849 0.10061331132065716 RNA PCR Primer, Index 31 (95% over 22bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4414987193432765E-5 2 0.0 0.0 0.0 0.0 5.4414987193432765E-5 3 0.0 0.0 0.0 0.0 5.4414987193432765E-5 4 0.0 0.0 0.0 5.4414987193432765E-5 5.4414987193432765E-5 5 0.0 0.0 0.0 5.4414987193432765E-5 1.0882997438686553E-4 6 0.0 0.0 0.0 5.4414987193432765E-5 1.0882997438686553E-4 7 0.0 0.0 0.0 5.4414987193432765E-5 1.0882997438686553E-4 8 0.0 0.0 0.0 5.4414987193432765E-5 1.0882997438686553E-4 9 0.0 0.0 0.0 5.4414987193432765E-5 1.0882997438686553E-4 10-11 0.0 0.0 0.0 5.4414987193432765E-5 1.0882997438686553E-4 12-13 0.0 0.0 0.0 8.162248079014915E-5 6.529798463211932E-4 14-15 0.0 0.0 0.0 1.9045245517701468E-4 7.890173143047751E-4 16-17 0.0 0.0 0.0 2.448674423704474E-4 8.162248079014914E-4 18-19 0.0 0.0 0.0 3.8090491035402935E-4 8.162248079014914E-4 20-21 0.0 0.0 0.0 5.985648591277605E-4 9.794697694817897E-4 22-23 0.0 0.0 0.0 0.0012243372118522372 9.794697694817897E-4 24-25 0.0 0.0 0.0 0.0029928242956388023 9.794697694817897E-4 26-27 0.0 0.0 0.0 0.007454853245500288 0.001142714731062088 28-29 0.0 0.0 0.0 0.01969822536402266 0.001142714731062088 30-31 0.0 0.0 0.0 0.051911897782534855 0.001142714731062088 32-33 0.0 0.0 0.0 0.11489724545893329 0.0011971297182555207 34-35 0.0 0.0 0.0 0.19948534305112453 0.0012787521990456699 36-37 0.0 0.0 0.0 0.32322502392899066 0.0013331671862391028 38-39 0.0 0.0 0.0 0.49642792816568715 0.001360374679835819 40-41 0.0 0.0 0.0 0.6819286195080994 0.001360374679835819 42-43 0.0 0.0 0.0 0.8614980772464276 0.001360374679835819 44-45 0.0 0.0 0.0 1.0811169655591222 0.0013875821734325353 46-47 0.0 0.0 0.0 1.3342554859829714 0.001496412147819401 48-49 0.0 0.0 0.0 1.5743072019867999 0.0016596571093996993 50-51 0.0 0.0 0.0 1.836669062739936 0.0016868646029964158 52-53 0.0 0.0 0.0 2.0754964415319126 0.0017412795901898485 54-55 0.0 0.0 0.0 2.33304257591843 0.0017956945773832812 56-57 0.0 0.0 0.0 2.637766504201653 0.001850109564576714 58-59 0.0 0.0 0.0 2.931797887501367 0.001931732045366863 60-61 0.0 0.0 0.0 3.2378549829708296 0.0020133545261570122 62-63 0.0 0.0 0.0 3.534253418213458 0.002067769513350445 64-65 0.0 0.0 0.0 3.8437658653697033 0.002067769513350445 66-67 0.0 0.0 0.0 4.201381161204944 0.002067769513350445 68-69 0.0 0.0 0.0 4.587346665367962 0.002067769513350445 70-71 0.0 0.0 0.0 4.976060126384249 0.002094977006947161 72-73 0.0 0.0 0.0 5.366297207041953 0.0021221845005438777 74-75 0.0 0.0 0.0 5.762111823886983 0.002149391994140594 76-77 0.0 0.0 0.0 6.168727815689909 0.002203806981334027 78-79 0.0 0.0 0.0 6.6461377058314905 0.002285429462124176 80-81 0.0 0.0 0.0 7.124880763159313 0.002339844449317609 82-83 0.0 0.0 0.0 7.603787065448714 0.0024486744237044744 84-85 0.0 0.0 0.0 8.102663668038105 0.0025030894108979073 86-87 0.0 0.0 0.0 8.6048323773527 0.0026119193852847727 88-89 0.0 0.0 0.0 9.125202899883497 0.002720749359671638 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 1190 0.0 29.543037 2 TAAGGTA 605 0.0 28.261766 4 GTGTAAG 645 0.0 25.779749 1 GAGTACT 12285 0.0 24.801256 12-13 TAAGGTG 1585 0.0 24.571789 5 GTACATG 9240 0.0 24.474022 1 GTACTTT 12615 0.0 24.039524 14-15 TACATGG 9305 0.0 23.537207 2 AAGGTAA 1455 0.0 23.50931 1 ACATGGG 9210 0.0 22.642689 3 GGTAAGG 1675 0.0 22.404507 3 TGTAAGG 830 0.0 22.323223 2 GTAAGGT 1725 0.0 22.302221 4 AGTACTT 12390 0.0 22.233553 12-13 GTATAGG 370 0.0 21.828182 1 AGGTGTG 2035 0.0 21.472153 7 AGAGTAC 17300 0.0 21.469051 10-11 ACTTTTT 14235 0.0 21.353777 16-17 GGTGTGC 1855 0.0 20.739262 8 TACTTTT 13430 0.0 20.405724 14-15 >>END_MODULE