##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938036_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1822626 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16742710792011 33.0 31.0 34.0 31.0 34.0 2 32.248367465404314 34.0 31.0 34.0 31.0 34.0 3 32.26569960046658 34.0 31.0 34.0 30.0 34.0 4 35.76566832690854 37.0 35.0 37.0 35.0 37.0 5 35.79379971535575 37.0 35.0 37.0 35.0 37.0 6 35.85751876687812 37.0 37.0 37.0 35.0 37.0 7 35.80483214877874 37.0 37.0 37.0 35.0 37.0 8 35.79993043004983 37.0 36.0 37.0 35.0 37.0 9 37.47707867659081 39.0 38.0 39.0 35.0 39.0 10-11 37.40230881157187 39.0 38.0 39.0 35.0 39.0 12-13 37.28720922449257 39.0 37.0 39.0 35.0 39.0 14-15 38.49507194564326 40.0 38.0 41.0 34.0 41.0 16-17 38.380483160011984 40.0 38.0 41.0 34.0 41.0 18-19 38.58285023916042 40.0 38.0 41.0 34.5 41.0 20-21 38.594832949820756 40.0 38.0 41.0 34.5 41.0 22-23 38.49964062841197 40.0 38.0 41.0 34.0 41.0 24-25 38.380085930958955 40.0 38.0 41.0 34.0 41.0 26-27 38.208815741682606 40.0 38.0 41.0 34.0 41.0 28-29 38.05400833742084 40.0 38.0 41.0 34.0 41.0 30-31 37.79930276425334 40.0 37.5 41.0 33.0 41.0 32-33 37.45417381294901 40.0 36.5 41.0 33.0 41.0 34-35 37.203308029184264 40.0 36.0 41.0 32.0 41.0 36-37 36.92818932682843 40.0 35.5 41.0 31.5 41.0 38-39 36.626497701667816 39.0 35.0 41.0 30.5 41.0 40-41 36.5050783868989 39.0 35.0 41.0 30.0 41.0 42-43 36.25230903103544 39.0 35.0 41.0 30.0 41.0 44-45 35.89222638105679 39.0 35.0 41.0 29.5 41.0 46-47 35.43576904971179 39.0 35.0 41.0 26.5 41.0 48-49 34.951361387360876 39.0 35.0 40.0 24.5 41.0 50-51 34.149161978376256 37.5 34.0 39.5 23.0 40.5 52-53 34.164613036355234 38.0 34.0 40.0 23.0 40.5 54-55 34.23618065362834 38.0 34.0 40.0 22.5 41.0 56-57 33.919135357445796 38.0 34.0 40.0 20.5 41.0 58-59 33.54453985622942 37.5 33.5 40.0 18.5 41.0 60-61 33.04319344725687 37.0 33.0 40.0 14.0 41.0 62-63 32.56984537694513 36.0 32.5 39.5 9.5 41.0 64-65 32.08702717946523 35.5 32.0 39.0 7.5 40.5 66-67 31.57791614955564 35.0 31.0 39.0 2.0 40.0 68-69 30.96781402218557 35.0 31.0 38.0 2.0 40.0 70-71 30.41270672096195 35.0 30.0 37.0 2.0 39.5 72-73 29.7950470913945 34.5 29.0 36.5 2.0 39.0 74-75 29.219723355202877 34.0 29.0 36.0 2.0 39.0 76-77 28.58747954873902 34.0 27.5 35.5 2.0 37.5 78-79 27.904287549941678 34.0 26.5 35.0 2.0 37.0 80-81 27.254414235284692 33.0 25.5 35.0 2.0 36.5 82-83 26.601231684393838 33.0 24.5 35.0 2.0 36.0 84-85 25.94772460175593 32.0 22.0 35.0 2.0 35.5 86-87 25.331746337427425 32.0 19.5 35.0 2.0 35.0 88-89 24.50620889858918 31.0 14.5 34.0 2.0 35.0 90-91 23.69777233508136 31.0 4.5 34.0 2.0 35.0 92-93 22.857874297853755 30.5 2.0 34.0 2.0 35.0 94-95 21.8468429617486 29.5 2.0 34.0 2.0 35.0 96-97 20.660572437790307 29.0 2.0 34.0 2.0 35.0 98-99 19.171869324809368 26.5 2.0 34.0 2.0 35.0 100-101 14.964368444211813 11.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11564891380913878 1101 2 0.21925879294018813 1101 3 0.0642211868267637 1101 4 0.01141748680946364 1101 5 0.06363128426211517 1101 6 0.10769994933198745 1101 7 0.14371727761638908 1101 8 0.03985688492723938 1101 9 0.13841759154351507 1101 10-11 0.2101053066596208 1101 12-13 0.2015452839809413 1101 14-15 0.19862319109439142 1101 16-17 0.30873088946586336 1101 18-19 0.31496173062036803 1101 20-21 0.3677937730176879 1101 22-23 0.30225358065533214 1101 24-25 0.3450650684178811 1101 26-27 0.34797928204058337 1101 28-29 0.30127963852139317 1101 30-31 0.41086033546402945 1101 32-33 0.18201096413616114 1101 34-35 0.25437788060981603 1101 36-37 0.18951121496792922 1101 38-39 0.08825164766690108 1101 40-41 0.28277375851337183 1101 42-43 0.175132058771581 1101 44-45 0.31197104225647365 1101 46-47 0.22612857615426663 1101 48-49 0.2699836230347614 1101 50-51 0.15572995418832392 1101 52-53 0.11432557546306299 1101 54-55 0.04361778809033723 1101 56-57 0.024122421675201622 1101 58-59 -0.08598399895799957 1101 60-61 -0.12066655675162963 1101 62-63 -0.22810047185637927 1101 64-65 -0.16994460522833066 1101 66-67 -0.1923064496068676 1101 68-69 -0.13201955930199105 1101 70-71 -0.12824840537098225 1101 72-73 -0.16559796176348485 1101 74-75 -0.14952044901138706 1101 76-77 -0.285480151734955 1101 78-79 -0.21564975292371358 1101 80-81 -0.2657324288319103 1101 82-83 -0.3090251404625093 1101 84-85 -0.25497342446492155 1101 86-87 -0.30233971093137413 1101 88-89 -0.2656005046512355 1101 90-91 -0.4579378723873795 1101 92-93 -0.5485211038550446 1101 94-95 -0.6259201905186025 1101 96-97 -0.6997620310073209 1101 98-99 -0.9874646737201296 1101 100-101 -1.3642711730218728 1102 1 0.27363359130248455 1102 2 0.2678641695802426 1102 3 0.33007126481557236 1102 4 0.22956823458449094 1102 5 0.15702670280204245 1102 6 0.15335780785111552 1102 7 0.2050837638383669 1102 8 0.19995949372927413 1102 9 0.18304706825391293 1102 10-11 0.26334795889071216 1102 12-13 0.338425196511146 1102 14-15 0.3935168357712726 1102 16-17 0.4539831297325563 1102 18-19 0.2667959967609974 1102 20-21 0.3075932959096903 1102 22-23 0.3589789683055713 1102 24-25 0.35669648720247693 1102 26-27 0.33991661574092547 1102 28-29 0.26098159894589656 1102 30-31 0.3198829522178599 1102 32-33 0.3356565585332021 1102 34-35 0.27388655113005456 1102 36-37 0.3377453801818646 1102 38-39 0.25113738641969263 1102 40-41 0.3659732875221451 1102 42-43 0.40553163671451387 1102 44-45 0.4742336351549028 1102 46-47 0.5486505200634966 1102 48-49 0.29225439711188983 1102 50-51 0.3968778423737902 1102 52-53 0.358520379267695 1102 54-55 0.18580399783434842 1102 56-57 0.06402095976737598 1102 58-59 0.24490262672630791 1102 60-61 0.2625853470927595 1102 62-63 -0.06302225367122816 1102 64-65 -0.16265418103039053 1102 66-67 -0.08163498127644075 1102 68-69 -0.18405988410242813 1102 70-71 -0.24468110561871015 1102 72-73 -0.06819032497723398 1102 74-75 -0.053095318265445 1102 76-77 -0.34040682696509705 1102 78-79 -0.3480357516391912 1102 80-81 -0.4322996980003353 1102 82-83 -0.34127198268045333 1102 84-85 -0.3670746875777411 1102 86-87 -0.3484486811732914 1102 88-89 -0.3734831085598529 1102 90-91 -0.3799089247775065 1102 92-93 -0.4479155437119111 1102 94-95 -0.5915401250691694 1102 96-97 -0.7528058882419337 1102 98-99 -0.88883750609007 1102 100-101 -1.2096602759976811 1103 1 0.34374493769723813 1103 2 0.37050427463121594 1103 3 0.4032416960205438 1103 4 0.22986790327968265 1103 5 0.3034323477017935 1103 6 0.2711939773062113 1103 7 0.3080593070574267 1103 8 0.2685160603685972 1103 9 0.373012777252562 1103 10-11 0.4000183455825592 1103 12-13 0.3327153950396635 1103 14-15 0.3824823508198918 1103 16-17 0.4207964114942584 1103 18-19 0.42600572192426256 1103 20-21 0.5306149658512496 1103 22-23 0.33221684483888936 1103 24-25 0.3661177689260242 1103 26-27 0.4345982117906999 1103 28-29 0.48651229869217616 1103 30-31 0.3474763184909051 1103 32-33 0.4121336787885852 1103 34-35 0.391479090730833 1103 36-37 0.3504334083777465 1103 38-39 0.32477202589520715 1103 40-41 0.4133201464855034 1103 42-43 0.616890073858805 1103 44-45 0.6939547763441709 1103 46-47 0.688048726502636 1103 48-49 0.6685295788208094 1103 50-51 0.8109224079575128 1103 52-53 0.7910029158161507 1103 54-55 0.6313368278514488 1103 56-57 0.7075821366943202 1103 58-59 0.7769327986741388 1103 60-61 0.8415957409067119 1103 62-63 0.7685893756998041 1103 64-65 0.5291000016133012 1103 66-67 0.5714285548459621 1103 68-69 0.5361008241649294 1103 70-71 0.4288099790388884 1103 72-73 0.37386193660730527 1103 74-75 0.41420244113477267 1103 76-77 0.391717776391193 1103 78-79 0.3265325903106131 1103 80-81 0.32953840777555854 1103 82-83 0.010637034088279762 1103 84-85 0.048370451589352825 1103 86-87 -0.3607257578694032 1103 88-89 -0.13167193322266613 1103 90-91 0.00659831337405592 1103 92-93 -0.21191690772114313 1103 94-95 -0.5682937433500754 1103 96-97 -0.8960874671223635 1103 98-99 -1.092250152267983 1103 100-101 -1.2759071282840662 1104 1 0.17738041392092185 1104 2 0.221466483528161 1104 3 0.36200790714870124 1104 4 0.30012193572517987 1104 5 0.3307152696559612 1104 6 0.23996576645696166 1104 7 0.2678975444022882 1104 8 0.2592193560502736 1104 9 0.4197258112320128 1104 10-11 0.3694176622747918 1104 12-13 0.41501592262763864 1104 14-15 0.4330622327517446 1104 16-17 0.4559568377481469 1104 18-19 0.4768310949545267 1104 20-21 0.4230419286179554 1104 22-23 0.3698815708185279 1104 24-25 0.39980089075934444 1104 26-27 0.42519256171865294 1104 28-29 0.44221786044553824 1104 30-31 0.34559373586645137 1104 32-33 0.6169865584427399 1104 34-35 0.5562166912514428 1104 36-37 0.5404594972542469 1104 38-39 0.42335485953068286 1104 40-41 0.5933827830308473 1104 42-43 0.6427712271149773 1104 44-45 0.586409338701813 1104 46-47 0.4457751593571899 1104 48-49 0.4689366001567521 1104 50-51 0.5688522062190273 1104 52-53 0.6763178997793347 1104 54-55 0.5290861538403888 1104 56-57 0.5938137175988913 1104 58-59 0.35322533109639664 1104 60-61 0.6680378921640866 1104 62-63 0.7472795862701531 1104 64-65 0.564424572802821 1104 66-67 0.5945163389764865 1104 68-69 0.6602060163742891 1104 70-71 0.5979141253690123 1104 72-73 0.6715685558607802 1104 74-75 0.5080918916995465 1104 76-77 0.477202425208187 1104 78-79 0.34332912031572604 1104 80-81 0.2630001518372893 1104 82-83 0.03542582458704402 1104 84-85 0.13981661205046692 1104 86-87 0.15592841635083587 1104 88-89 0.18816374752850606 1104 90-91 0.03145682915521064 1104 92-93 -0.24503351163505727 1104 94-95 -0.534394182619355 1104 96-97 -0.5502398982105419 1104 98-99 -0.6682158474290496 1104 100-101 -0.8670424301945392 1105 1 0.004026429763975159 1105 2 0.057133117927911314 1105 3 0.2569065083780657 1105 4 0.11052156142900316 1105 5 0.06773590434612231 1105 6 0.18757640163111944 1105 7 0.15004466538707106 1105 8 0.16050680583489196 1105 9 0.20126873429155978 1105 10-11 0.13122432700544095 1105 12-13 0.23529930678135713 1105 14-15 0.28003035304278967 1105 16-17 0.2180781048697753 1105 18-19 0.1898935475624981 1105 20-21 0.21710239681085142 1105 22-23 0.31718567032968537 1105 24-25 0.17907000487127078 1105 26-27 0.178346652356403 1105 28-29 0.15845658719318578 1105 30-31 0.12873588774680655 1105 32-33 0.1711153114879309 1105 34-35 0.176056002030208 1105 36-37 0.11325869219479046 1105 38-39 0.11459076292354808 1105 40-41 0.177628664966214 1105 42-43 0.13338357243546284 1105 44-45 0.20065538197174249 1105 46-47 0.1973007792852357 1105 48-49 0.2872305275239313 1105 50-51 0.3086679621521071 1105 52-53 0.37829969317429857 1105 54-55 0.40741872083456343 1105 56-57 0.3161811409246411 1105 58-59 0.33365544903196565 1105 60-61 0.42238081683697715 1105 62-63 0.4373286723098744 1105 64-65 0.1282949082173701 1105 66-67 0.17421157799363485 1105 68-69 0.3915547329578679 1105 70-71 0.3085868098147486 1105 72-73 0.15762723324006345 1105 74-75 0.09085551236973188 1105 76-77 0.1313535634235059 1105 78-79 -0.08434963837617104 1105 80-81 0.08071932483633759 1105 82-83 0.03428337423775929 1105 84-85 0.19295517423687514 1105 86-87 0.07199209510146076 1105 88-89 0.048740437911352075 1105 90-91 -0.22795300902622628 1105 92-93 -0.42157788670564145 1105 94-95 -0.45429661554451073 1105 96-97 -0.5834758258035926 1105 98-99 -0.8158581445105035 1105 100-101 -1.3924546399939999 1106 1 0.23361201004679089 1106 2 0.19866785295378264 1106 3 0.2360455840919684 1106 4 0.23473422744358174 1106 5 0.2255633674788342 1106 6 0.187769718633497 1106 7 0.2813833712018763 1106 8 0.20207717466642094 1106 9 0.370688725577871 1106 10-11 0.39505226333201904 1106 12-13 0.42983067174365885 1106 14-15 0.40276753425006717 1106 16-17 -0.0678225936682253 1106 18-19 0.3197329646107434 1106 20-21 0.540622728277377 1106 22-23 0.4992718925980739 1106 24-25 0.4696993959804203 1106 26-27 0.43979888141685564 1106 28-29 0.4459193303903106 1106 30-31 0.4264909579901186 1106 32-33 0.32812334135685717 1106 34-35 0.2896073796302474 1106 36-37 0.4153626736594447 1106 38-39 0.5002387066942688 1106 40-41 0.4881917079489497 1106 42-43 0.26867589948167137 1106 44-45 0.4015716180031461 1106 46-47 0.42556415783166557 1106 48-49 0.3450164419277755 1106 50-51 0.2111947163596355 1106 52-53 0.2159217064987402 1106 54-55 0.22169193546549337 1106 56-57 0.06443551724647989 1106 58-59 0.34555778932182335 1106 60-61 0.38083028463815793 1106 62-63 0.21897531479748977 1106 64-65 0.10004826457561933 1106 66-67 0.06592159671941644 1106 68-69 0.08158895295261814 1106 70-71 0.022035469723974188 1106 72-73 0.09566624865060191 1106 74-75 -0.04337465316156042 1106 76-77 -0.49198845474829156 1106 78-79 -0.44182523571128485 1106 80-81 -0.4318776582642947 1106 82-83 -0.2988912660339267 1106 84-85 -0.28259960228001546 1106 86-87 -0.3050568487240817 1106 88-89 -0.3353872984619599 1106 90-91 -0.3566949192867419 1106 92-93 -0.1888743484274329 1106 94-95 -0.1630771747788593 1106 96-97 0.14589587951204663 1106 98-99 -0.011971390764625056 1106 100-101 -0.24832635540828996 1107 1 -0.0035311193882350267 1107 2 -0.032947414468331715 1107 3 0.07807149836494176 1107 4 0.10876482476373894 1107 5 -0.013581109424265492 1107 6 0.027708248675544667 1107 7 0.09876041474044683 1107 8 -0.05548512587719756 1107 9 0.042917448355510146 1107 10-11 -0.007452531562954334 1107 12-13 0.03358488589048392 1107 14-15 -0.026901217594755167 1107 16-17 0.03029538523556141 1107 18-19 0.14555468187806042 1107 20-21 0.17064578612981762 1107 22-23 0.20220895604423816 1107 24-25 0.2290325647408693 1107 26-27 0.1204094049042368 1107 28-29 0.04667086151377475 1107 30-31 0.025815106126891862 1107 32-33 0.191790148770842 1107 34-35 0.09860775157379464 1107 36-37 0.11555235163045552 1107 38-39 -0.01367840151748112 1107 40-41 0.03736932886038602 1107 42-43 -0.018418850819784893 1107 44-45 -0.11720094182880558 1107 46-47 -0.05507459233214007 1107 48-49 -0.10685227656126273 1107 50-51 -0.1956369004299745 1107 52-53 -0.2767338976050766 1107 54-55 -0.2546117200380209 1107 56-57 -0.40237712512781343 1107 58-59 -0.387549057748501 1107 60-61 -0.2751912629381863 1107 62-63 -0.308949736592929 1107 64-65 -0.32012484139122677 1107 66-67 -0.15984482538224043 1107 68-69 -0.24104592952778248 1107 70-71 -0.29185807765117744 1107 72-73 -0.07651052115870272 1107 74-75 -0.20259610649455695 1107 76-77 -0.35514963072524 1107 78-79 -0.3339060149661002 1107 80-81 -0.261925435140526 1107 82-83 -0.2254023032269572 1107 84-85 -0.4631510582205891 1107 86-87 -0.2773037054040408 1107 88-89 -0.028413919560026812 1107 90-91 -0.053202554718534856 1107 92-93 -0.3732089401035772 1107 94-95 -0.5580257909768029 1107 96-97 -0.5430201697856951 1107 98-99 -0.5880843093831167 1107 100-101 -0.5391400447838084 1108 1 0.05261348239285013 1108 2 0.14787063142982504 1108 3 0.06298464332952847 1108 4 0.12190224418831264 1108 5 0.1127185589094779 1108 6 0.1402339744831167 1108 7 0.14704974887146705 1108 8 0.1137248684184442 1108 9 0.20033905621446735 1108 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-0.5954763611920946 2316 72-73 -0.618867873445943 2316 74-75 -0.5922561301025802 2316 76-77 -0.7516380872369943 2316 78-79 -0.4840802556698023 2316 80-81 -0.4729819107962214 2316 82-83 -0.6501566712328213 2316 84-85 -0.6355360212822703 2316 86-87 -0.5021883238028018 2316 88-89 -0.43120720994425454 2316 90-91 -0.39569437549559083 2316 92-93 -0.5125201381962832 2316 94-95 -0.38001166656825447 2316 96-97 -0.36017454835900153 2316 98-99 -0.4719650961958344 2316 100-101 -0.10683384025183962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7776.0 3 1466.0 4 1127.0 5 1229.0 6 1305.0 7 1675.0 8 2132.0 9 2803.0 10 3625.0 11 4924.0 12 7237.0 13 9642.0 14 11425.0 15 15520.0 16 19706.0 17 26645.0 18 25227.0 19 19038.0 20 17850.0 21 17623.0 22 18682.0 23 20451.0 24 23009.0 25 25267.0 26 28901.0 27 33736.0 28 39742.0 29 47074.0 30 56064.0 31 68751.0 32 85439.0 33 109736.0 34 148073.0 35 208394.0 36 265061.0 37 289349.0 38 147215.0 39 9707.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08862071258119 18.69037944511571 14.931149093902185 21.289850748400916 2 15.720431724895874 21.158604167821775 38.858040329969484 24.26292377731287 3 19.54844910229985 25.261715875827072 30.93871526642514 24.251119755447938 4 12.580031270842193 17.18605272092586 37.45304154539052 32.780874462841425 5 12.590885152392397 38.366920388289735 34.05792241461771 14.984272044700159 6 30.596804457025428 37.06991480347955 16.689765807151407 15.643514932343617 7 26.9807658276058 35.08348657433004 20.600646085292492 17.33510151277167 8 27.04297919699032 32.22371869240107 19.863417965951125 20.869884144657483 9 26.804558653718324 16.903341203562807 20.84711722173941 35.44498292097945 10-11 24.90961137422017 26.439338975333776 26.660346923161843 21.990702727284212 12-13 27.273756327437614 24.623321017313497 26.944993329127865 21.157929326121025 14-15 23.247683063784148 25.861282715666817 26.995740837195807 23.895293383353224 16-17 20.845222400995098 29.014361380224262 28.03923577755258 22.101180441228056 18-19 20.909172318994568 26.92844828628157 31.303833547413078 20.85854584731078 20-21 21.906510837661028 25.396456075916507 31.561581896072184 21.135451190350285 22-23 21.526218095789662 25.439368772362187 32.10468492354699 20.929728208301164 24-25 21.868618388279113 25.889315566816336 31.5920640104866 20.650002034417952 26-27 20.770107612966964 25.80048975178052 32.192978576038314 21.236424059214194 28-29 20.78199078168863 27.428761351213264 31.227167099747845 20.562080767350256 30-31 20.899677943053476 26.12286055109963 30.837694063475535 22.139767442371358 32-33 20.545434019914318 26.391677198074987 31.16947112050247 21.89341766150822 34-35 22.222231375162455 26.27070498050836 30.57094851278908 20.93611513154011 36-37 22.13694933571442 25.36852356911648 31.16663138417472 21.327895710994383 38-39 21.11146822947273 26.28087087719279 30.855602834854963 21.75205805847952 40-41 21.559129734687062 26.367087832739177 30.908368723306545 21.16541370926722 42-43 21.202502805417144 26.508666597858 29.932291174849407 22.356539421875446 44-45 21.29222824859439 26.054208807895275 30.740746127873685 21.91281681563665 46-47 22.465685445710434 27.001755526229694 29.564752458724826 20.96780656933505 48-49 21.611279378298182 27.07831321994601 29.201965268042496 22.108442133713314 50-51 21.184033377021734 27.419305697561914 29.828682005640605 21.567978919775747 52-53 22.225490723371745 27.1603573008376 28.908115115146977 21.706036860643675 54-55 21.080793996717297 27.1096301745102 29.44737521065835 22.362200618114148 56-57 21.77888344925596 27.036905627262303 28.833576045053004 22.350634878428732 58-59 22.233823353021403 26.44687281363744 28.66676932020576 22.6525345131354 60-61 21.000927058753792 27.118307297198353 29.908046090572928 21.972719553474935 62-63 21.06051485924661 27.1803731081301 29.9773254206984 21.781786611924893 64-65 20.66480373639921 27.801270239606644 29.119372992780455 22.41455303121369 66-67 20.822221947782317 27.156651668526 28.886693369669175 23.134433014022505 68-69 21.56967546248675 26.711473437809264 29.211011561859944 22.507839537844042 70-71 21.945126752924367 27.008820924285303 28.30315301999386 22.74289930279647 72-73 21.510821243017745 26.883441937802726 29.073271443287112 22.532465375892414 74-75 21.313406013380572 28.407848083826043 28.212301925464118 22.06644397732927 76-77 21.71608288875334 27.142442030363423 28.46261343615266 22.678861644730578 78-79 21.842129380897056 27.73571631230583 28.27004265720412 22.152111649592992 80-81 22.745830958622264 27.66394561593808 27.674455170165597 21.915768255274052 82-83 22.299072862437416 27.387641952419067 28.32015756591611 21.99312761922741 84-85 21.89021636355355 28.04741592431803 28.137386768167165 21.924980943961252 86-87 22.592388521591573 28.10529649006212 27.284461540065713 22.017853448280594 88-89 22.03785692638126 28.137504227554434 26.753913507530534 23.070725338533773 90-91 22.26836394735028 27.650687397578324 27.085104156682544 22.995844498388855 92-93 23.067691707051356 27.38731387601609 27.432780114999932 22.112214301932617 94-95 22.505162017762117 26.80781809930676 27.849391251158895 22.837628631772223 96-97 22.39787105778385 26.45305137769618 28.26742338476337 22.8816541797566 98-99 22.536722100014988 27.32035307674023 26.854852389701943 23.28807243354284 100-101 23.353266930738762 25.490328039072413 27.3947511550865 23.761653875102322 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3350.0 1 2151.5 2 1264.0 3 1599.5 4 1384.0 5 1336.0 6 2261.5 7 4672.5 8 7333.0 9 8001.0 10 6285.5 11 4464.5 12 4073.0 13 4122.0 14 4173.5 15 4233.5 16 4288.5 17 4328.0 18 4542.0 19 4927.5 20 5298.0 21 5884.0 22 6770.5 23 7860.5 24 9195.0 25 10993.5 26 13420.5 27 15810.5 28 17763.5 29 20025.0 30 22335.5 31 26835.5 32 34676.5 33 36509.0 34 32841.0 35 33110.0 36 36516.0 37 40020.5 38 42038.0 39 43144.0 40 44966.5 41 47607.0 42 49060.5 43 50693.0 44 52933.5 45 57641.5 46 61231.5 47 68070.5 48 81809.5 49 80581.5 50 66592.5 51 57883.5 52 55251.0 53 56401.5 54 58538.5 55 56733.0 56 51994.5 57 47743.0 58 46070.5 59 41537.5 60 32603.0 61 27322.0 62 24450.0 63 18555.0 64 12148.5 65 8783.0 66 6692.5 67 5316.0 68 4184.5 69 3170.5 70 2381.5 71 1586.0 72 1105.0 73 841.0 74 592.0 75 434.5 76 307.0 77 213.5 78 155.0 79 91.5 80 54.5 81 44.0 82 29.5 83 11.0 84 8.0 85 9.0 86 6.0 87 5.0 88 5.0 89 4.5 90 3.0 91 2.0 92 1.5 93 0.5 94 2.0 95 3.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14698572279776542 2 0.05503048897579646 3 0.15411828866701122 4 0.13167813912453788 5 0.19598096373035387 6 0.20053483270841083 7 0.1674507002533707 8 0.07149025636636369 9 0.12421637790748075 10-11 0.102983277973649 12-13 0.15219798247144506 14-15 0.1399080228198215 16-17 0.1372470270916798 18-19 0.1334338476461984 20-21 0.1354364526787174 22-23 0.19573406721949538 24-25 0.21529375746861945 26-27 0.14948212085200147 28-29 0.12816672208121688 30-31 0.10040458108246013 32-33 0.12495706744005627 34-35 0.09423216831099743 36-37 0.08830665205039322 38-39 0.05187570022593774 40-41 0.06230021957329698 42-43 0.0630957749971744 44-45 0.03986007003082366 46-47 0.05212259673679625 48-49 0.04323432234588994 50-51 0.05560658083446631 52-53 0.02853026347698321 54-55 0.05245179208460759 56-57 0.027734708053105794 58-59 0.052918152160673665 60-61 0.04032643010688973 62-63 0.0539057382041077 64-65 0.04197240684594645 66-67 0.040244131269936895 68-69 0.06855493118171255 70-71 0.054399531225824714 72-73 0.05236949324765476 74-75 0.04419447544367303 76-77 0.02197378946640726 78-79 0.023318003803303587 80-81 0.026033865422747177 82-83 0.022193253031614825 84-85 0.0202455138903977 86-87 0.025073712324964092 88-89 0.06814343699694836 90-91 0.11124059461458356 92-93 0.08199707455067579 94-95 0.07629102185527914 96-97 0.06869209590996726 98-99 0.057005661062664525 100-101 0.13255599338536814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1822626.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.154007673681015 #Duplication Level Percentage of deduplicated Percentage of total 1 68.66697946257135 26.885874408060157 2 14.89465315332482 11.663707277241944 3 6.197137100007499 7.279282607056409 4 3.154433114462806 4.9403479347916095 5 1.8448909677131233 3.6117437553472223 6 1.1824453236623576 2.7778483965830514 7 0.8217918521273447 2.2523511139053816 8 0.5648182392708829 1.7691918139717722 9 0.40654501232766527 1.4326079879136737 >10 2.0156477760593012 14.201973054410619 >50 0.15465796768681184 4.167508198131293 >100 0.07873721509923166 6.110899881601172 >500 0.006736708560748672 1.8663864637971304 >1k 0.009824366651091813 7.8458679820269355 >5k 2.806961900311947E-4 0.8735154642715576 >10k+ 4.21044285046792E-4 2.320893660890113 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16970 0.9310741753930867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13444 0.7376170426626197 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11821 0.648569701079651 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8972 0.4922567767605641 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6924 0.3798914313742918 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4955 0.2718604914008689 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3851 0.2112885474035814 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3789 0.20788686214286417 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3633 0.19932778309976923 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3577 0.19625529318686336 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3417 0.18747675057856084 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3287 0.18034418470931501 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3266 0.17919200099197533 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3163 0.17354081418788056 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3152 0.17293728938355976 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3053 0.16750556614467257 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2963 0.1625676359275024 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2937 0.16114112275365325 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2892 0.15867215764506815 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2892 0.15867215764506815 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2788 0.15296610494967153 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2765 0.15170418944972802 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2734 0.15000334681936942 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2683 0.14720518636297297 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2640 0.1448459530369917 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2604 0.14287078095012362 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2472 0.135628483298274 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2311 0.1267950747986696 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2292 0.1257526228639337 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2287 0.12547829340742422 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2135 0.11713867792953685 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 2115 0.11604136010349901 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2035 0.11165208879934775 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1972 0.10819553764732863 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1892 0.10380626634317737 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.486589130189079E-5 2 0.0 0.0 0.0 0.0 5.486589130189079E-5 3 0.0 0.0 0.0 0.0 5.486589130189079E-5 4 0.0 0.0 0.0 0.0 5.486589130189079E-5 5 0.0 0.0 0.0 0.0 5.486589130189079E-5 6 0.0 0.0 0.0 0.0 1.6459767390567236E-4 7 0.0 0.0 0.0 0.0 1.6459767390567236E-4 8 0.0 0.0 0.0 0.0 1.6459767390567236E-4 9 0.0 0.0 0.0 0.0 1.6459767390567236E-4 10-11 0.0 0.0 0.0 0.0 1.6459767390567236E-4 12-13 0.0 0.0 0.0 2.7432945650945395E-5 3.566282934622901E-4 14-15 0.0 0.0 0.0 1.3716472825472698E-4 4.1149418476418094E-4 16-17 0.0 0.0 0.0 1.6459767390567236E-4 4.937930217170171E-4 18-19 0.0 0.0 0.0 2.1946356520756316E-4 4.937930217170171E-4 20-21 0.0 0.0 0.0 4.663600760660717E-4 7.406895325755257E-4 22-23 0.0 0.0 0.0 0.0010698848803868703 8.778542608302526E-4 24-25 0.0 0.0 0.0 0.0029078922390002116 8.778542608302526E-4 26-27 0.0 0.0 0.0 0.00696796819534013 9.052872064811981E-4 28-29 0.0 0.0 0.0 0.018819000716548538 9.327201521321434E-4 30-31 0.0 0.0 0.0 0.05138190720422073 9.327201521321434E-4 32-33 0.0 0.0 0.0 0.11357239499491392 9.327201521321434E-4 34-35 0.0 0.0 0.0 0.1990808865889107 0.001042451934735925 36-37 0.0 0.0 0.0 0.31772837652924957 0.0010698848803868703 38-39 0.0 0.0 0.0 0.49489033954305495 0.0011247507716887612 40-41 0.0 0.0 0.0 0.6787733742413419 0.0011521837173397065 42-43 0.0 0.0 0.0 0.8627935736678836 0.0011521837173397065 44-45 0.0 0.0 0.0 1.0928462558967116 0.0012344825542925427 46-47 0.0 0.0 0.0 1.343940007439815 0.001316781391245379 48-49 0.0 0.0 0.0 1.5896569016353328 0.0013716472825472698 50-51 0.0 0.0 0.0 1.8463469740912286 0.0013716472825472698 52-53 0.0 0.0 0.0 2.0855348272218217 0.0014265131738491605 54-55 0.0 0.0 0.0 2.3463398415253596 0.0014265131738491605 56-57 0.0 0.0 0.0 2.6406404824687018 0.001536244956452942 58-59 0.0 0.0 0.0 2.9308536145100534 0.0016459767390567238 60-61 0.0 0.0 0.0 3.2346734875942733 0.0016459767390567238 62-63 0.0 0.0 0.0 3.5298794157440967 0.0017557085216605053 64-65 0.0 0.0 0.0 3.832821434567487 0.0017557085216605053 66-67 0.0 0.0 0.0 4.178750879225908 0.0017557085216605053 68-69 0.0 0.0 0.0 4.560535183850115 0.0017557085216605053 70-71 0.0 0.0 0.0 4.946297265593709 0.0018380073586133413 72-73 0.0 0.0 0.0 5.3377928329783515 0.001975172086868068 74-75 0.0 0.0 0.0 5.729370699199945 0.002112336815122795 76-77 0.0 0.0 0.0 6.1296722421385414 0.0021946356520756317 78-79 0.0 2.7432945650945395E-5 0.0 6.605908178638953 0.0021946356520756317 80-81 0.0 5.486589130189079E-5 0.0 7.0873838077586955 0.0021946356520756317 82-83 0.0 5.486589130189079E-5 0.0 7.558873844661495 0.0021946356520756317 84-85 0.0 5.486589130189079E-5 0.0 8.051295219095962 0.0021946356520756317 86-87 0.0 5.486589130189079E-5 0.0 8.534691154411274 0.0022220685977265772 88-89 0.0 5.486589130189079E-5 0.0 9.043709460964564 0.0022495015433775223 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTA 520 0.0 33.848415 4 AGGTAAG 1215 0.0 33.671474 2 TAAGGTG 1570 0.0 29.996838 5 GTGTAAG 605 0.0 29.124908 1 AAGGTAA 1415 0.0 28.94411 1 GGTAAGG 1610 0.0 27.478754 3 GTAAGGT 1715 0.0 26.07376 4 GAGTACT 12110 0.0 24.079088 12-13 GTACATG 9530 0.0 23.536736 1 AAGGTGT 1995 0.0 23.362299 6 GGTGTGC 1915 0.0 23.33396 8 GTACTTT 12330 0.0 23.261734 14-15 TACATGG 9410 0.0 23.153456 2 AGGTGTG 2045 0.0 22.783571 7 AGTACTT 12235 0.0 22.453989 12-13 GTAAGCG 405 0.0 22.279743 94-95 ACATGGG 9445 0.0 21.707771 3 TGTAAGG 835 0.0 21.648962 2 GTGTGCA 2135 0.0 21.596298 9 AGAGTAC 17295 0.0 21.42255 10-11 >>END_MODULE