FastQCFastQC Report
Thu 26 May 2016
SRR938035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118950.682187312176255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97170.5572773528723556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.35964662754580035No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34370.19711456846992756No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.13827268681437163No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20030.11487357598058331No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17810.1021417068504338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.10133879618456851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88750.030.247581
GTATCAA129000.030.0135211
TATCAAC152650.025.3160322
ATCAACG154400.024.9675253
TCAACGC155850.024.765734
CAACGCA156000.024.7114475
AACGCAG159900.024.1336046
GAGTACT87800.023.86144412-13
GTACATG87150.023.8544651
TACATGG87250.023.6987672
TAAGGTG9200.023.2501955
GTACTTT92750.022.51049814-15
ACATGGG89350.022.343783
TATAACG1302.9282615E-421.9386462
GTAAGGT9200.021.7001824
ACGCAGA176300.021.6949447
CGCAGAG177150.021.5103868
AGTACTT90400.021.20448712-13
AGAGTAC137250.020.54286410-11
GCAGAGT183700.020.4312769
Produced by FastQC (version 0.11.5)