FastQCFastQC Report
Thu 26 May 2016
SRR938035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114930.6591323059135518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98640.565707914863941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62080.35603352954940654No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35170.20170262941772918No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.14383571071358112No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20280.11630734502677134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.1049518941809623No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17470.10019178094761812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91700.031.4303871
GTATCAA128800.028.5436861
GTACATG82050.024.4613441
GTGTAAG7000.024.4596881
TAAGGTG9550.024.3971655
TATCAAC151300.024.2360532
ATCAACG151350.024.161733
TCAACGC152100.024.0412084
CAACGCA154500.023.6670725
TACATGG84250.023.483882
GAGTACT90900.023.17210812-13
AACGCAG158050.023.138336
AGGTAAG8800.022.1588732
GTACTTT95500.021.95650314-15
ACATGGG85650.021.7642063
GTAAGGT10000.021.3979264
ACGCAGA174850.020.9151467
CGCAGAG175300.020.7530848
GTGTAGC8700.020.2268811
AGTACTT94400.019.69679612-13
Produced by FastQC (version 0.11.5)