##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938035_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1743656 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4853606445308 33.0 31.0 34.0 31.0 34.0 2 32.70363362956913 34.0 31.0 34.0 31.0 34.0 3 32.76154470835991 34.0 31.0 34.0 31.0 34.0 4 36.22370869024624 37.0 35.0 37.0 35.0 37.0 5 36.15295448184734 37.0 35.0 37.0 35.0 37.0 6 36.217463192281045 37.0 37.0 37.0 35.0 37.0 7 36.19104399032837 37.0 37.0 37.0 35.0 37.0 8 36.19049342301463 37.0 37.0 37.0 35.0 37.0 9 37.8785448505898 39.0 38.0 39.0 35.0 39.0 10-11 37.85295149960772 39.0 38.0 39.0 35.0 39.0 12-13 37.768765169276506 39.0 38.0 39.0 35.0 39.0 14-15 38.98870906876127 40.0 38.0 41.0 35.5 41.0 16-17 38.90552695027 40.0 38.0 41.0 35.5 41.0 18-19 39.17896305234519 40.0 39.0 41.0 36.0 41.0 20-21 39.16319818817473 40.0 39.0 41.0 36.0 41.0 22-23 39.057043935271636 40.0 39.0 41.0 35.5 41.0 24-25 38.93600314511578 40.0 38.0 41.0 35.0 41.0 26-27 38.76232783301293 40.0 38.0 41.0 35.0 41.0 28-29 38.63875586698293 40.0 38.0 41.0 34.5 41.0 30-31 38.38303398147341 40.0 38.0 41.0 34.0 41.0 32-33 38.12589897319196 40.0 37.5 41.0 34.0 41.0 34-35 38.003696830108694 40.0 37.0 41.0 33.0 41.0 36-37 37.931035708878355 40.0 37.0 41.0 33.0 41.0 38-39 37.73275749345054 40.0 37.0 41.0 33.0 41.0 40-41 37.50760557128241 40.0 37.0 41.0 33.0 41.0 42-43 37.22556226686915 40.0 36.0 41.0 32.0 41.0 44-45 36.91361140041384 40.0 36.0 41.0 31.0 41.0 46-47 36.475163965828116 39.0 35.5 41.0 30.5 41.0 48-49 35.99648439829875 39.0 35.0 41.0 29.5 41.0 50-51 35.584422041962405 39.0 35.0 40.0 28.0 41.0 52-53 35.049010814059656 38.0 34.5 40.0 26.5 41.0 54-55 34.6599128497823 38.0 34.0 40.0 25.5 41.0 56-57 34.269510155672904 38.0 34.0 40.0 24.0 41.0 58-59 33.758077854806224 37.0 33.0 40.0 22.5 41.0 60-61 33.25808473689764 36.5 32.5 39.5 21.5 41.0 62-63 32.708895561968646 36.0 32.0 39.0 19.5 40.0 64-65 32.13726073262157 35.5 31.0 39.0 16.5 40.0 66-67 31.567037879031187 35.0 31.0 38.0 10.5 40.0 68-69 30.9373448661892 35.0 30.0 38.0 7.5 40.0 70-71 30.300460354565352 34.5 29.0 37.0 4.5 39.0 72-73 29.637022153452286 34.0 28.5 36.5 2.0 39.0 74-75 28.989773212147348 34.0 27.5 36.0 2.0 38.5 76-77 27.690735156475817 31.5 26.0 34.5 2.0 36.5 78-79 28.343910725510078 33.5 27.0 35.0 2.0 37.0 80-81 28.059135517556214 33.5 26.5 35.0 2.0 37.0 82-83 27.49758524617241 33.0 26.0 35.0 2.0 36.0 84-85 26.846310281385776 33.0 25.0 35.0 2.0 36.0 86-87 26.14275981042132 32.0 23.5 35.0 2.0 35.0 88-89 25.329714404676153 32.0 20.0 34.0 2.0 35.0 90-91 24.674597225599545 31.0 17.5 34.0 2.0 35.0 92-93 23.745583417830122 31.0 4.5 34.0 2.0 35.0 94-95 22.848297485283794 30.5 2.0 34.0 2.0 35.0 96-97 21.79923333501562 30.0 2.0 34.0 2.0 35.0 98-99 20.39628831604399 29.0 2.0 34.0 2.0 35.0 100-101 16.560328413402644 14.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2348234824215396 1101 2 0.1976907550988969 1101 3 0.24572953202141434 1101 4 0.0709501851681793 1101 5 -0.0016461382268886382 1101 6 0.11038765042657417 1101 7 0.10019658662090336 1101 8 0.13794306141180357 1101 9 0.08668942366828247 1101 10-11 0.18357085561608244 1101 12-13 0.26974047252414124 1101 14-15 0.31950966011019233 1101 16-17 0.31790037057960774 1101 18-19 0.3064397117370916 1101 20-21 0.27427100447656727 1101 22-23 0.2859127268660586 1101 24-25 0.37989969917720856 1101 26-27 0.3813188212493941 1101 28-29 0.32974169607762605 1101 30-31 0.3824444165666563 1101 32-33 0.45004647794900166 1101 34-35 0.6039911499201551 1101 36-37 0.5678907750788724 1101 38-39 0.5845111462630044 1101 40-41 0.7941171781292766 1101 42-43 0.9144415658337479 1101 44-45 0.7917586363024043 1101 46-47 0.8515750118925638 1101 48-49 1.0201166078915733 1101 50-51 1.2446211764331636 1101 52-53 1.2901707651106875 1101 54-55 1.463459092091405 1101 56-57 1.3605066946114235 1101 58-59 1.4618543422870687 1101 60-61 1.4724754959048738 1101 62-63 1.4326503851898735 1101 64-65 1.5269498545735942 1101 66-67 1.8863081568664768 1101 68-69 2.167845882809406 1101 70-71 2.3138705695029564 1101 72-73 2.449306477270053 1101 74-75 2.4252331302106747 1101 76-77 2.436984846736898 1101 78-79 2.5405791668131243 1101 80-81 2.4995048957865755 1101 82-83 2.571407915854927 1101 84-85 2.6789378349386546 1101 86-87 3.0128628952045275 1101 88-89 3.4533168142122044 1101 90-91 3.7878241034022615 1101 92-93 3.9832563476308813 1101 94-95 4.288810091898647 1101 96-97 4.500270063450667 1101 98-99 5.083432258239373 1101 100-101 5.052608672039764 1102 1 0.19204578496615454 1102 2 0.183401832015619 1102 3 0.15435607076893376 1102 4 0.12838514124330658 1102 5 0.02017602111438066 1102 6 0.04490223926772785 1102 7 0.026081538565165374 1102 8 0.1531373325893739 1102 9 0.18415978470595462 1102 10-11 0.24608849785555975 1102 12-13 0.2775013367012704 1102 14-15 0.34299535354487176 1102 16-17 0.25504322280477254 1102 18-19 0.24468972206425832 1102 20-21 0.2473291532978905 1102 22-23 0.3284892597615112 1102 24-25 0.3058182144063366 1102 26-27 0.30296468728541726 1102 28-29 0.22765294098289246 1102 30-31 0.3126859259161847 1102 32-33 0.2602189932332024 1102 34-35 0.23176700569786135 1102 36-37 0.3541412742251495 1102 38-39 0.4197567262365638 1102 40-41 0.5674307521543938 1102 42-43 0.6311170966500086 1102 44-45 0.563665564452883 1102 46-47 0.5110036270886411 1102 48-49 0.5516069296171793 1102 50-51 0.7011078836511544 1102 52-53 0.8532551105061543 1102 54-55 0.8630586132551983 1102 56-57 1.024645787748689 1102 58-59 1.1880013890622365 1102 60-61 1.1137199106300386 1102 62-63 1.3093991183910205 1102 64-65 1.2448670117805776 1102 66-67 1.291829016017715 1102 68-69 1.5558553132318558 1102 70-71 1.695379480124796 1102 72-73 1.8691003384591944 1102 74-75 2.0079868514323067 1102 76-77 1.7897093679007021 1102 78-79 1.85533844921288 1102 80-81 1.9694300410553573 1102 82-83 2.240235111410115 1102 84-85 2.2693859392154785 1102 86-87 2.4338022110643003 1102 88-89 2.7865064278385496 1102 90-91 3.153096990120151 1102 92-93 3.311550454950776 1102 94-95 3.5245577441898988 1102 96-97 3.8523882939942915 1102 98-99 4.044897089080418 1102 100-101 4.1560128497220745 1103 1 0.18316613915929025 1103 2 0.20623130707915038 1103 3 0.24259910205864088 1103 4 0.1491067965270858 1103 5 0.040772762431615206 1103 6 0.10586521840792784 1103 7 0.15792037142200854 1103 8 0.12630399263808556 1103 9 0.23910042161828216 1103 10-11 0.23021615165692566 1103 12-13 0.29873505652337684 1103 14-15 0.38927142830132766 1103 16-17 0.36573023307583696 1103 18-19 0.3394150436970378 1103 20-21 0.35016891928687954 1103 22-23 0.38217124369310795 1103 24-25 0.45783354821615774 1103 26-27 0.4827229167168028 1103 28-29 0.5129274869909395 1103 30-31 0.4651583124843697 1103 32-33 0.6079223961874547 1103 34-35 0.6918201809943483 1103 36-37 0.7023592234521772 1103 38-39 0.5612850775707443 1103 40-41 0.6123699992954386 1103 42-43 0.8119784570375614 1103 44-45 0.8799469004863383 1103 46-47 0.9500315482555095 1103 48-49 1.0196889294318723 1103 50-51 1.1119759778114755 1103 52-53 1.2091738816836184 1103 54-55 1.3084228160559164 1103 56-57 1.3716435508787157 1103 58-59 1.5243529250904047 1103 60-61 1.4901274676768352 1103 62-63 1.5280829388060937 1103 64-65 1.7128778685414758 1103 66-67 1.8246867663089148 1103 68-69 1.875594946199886 1103 70-71 1.9117341927730358 1103 72-73 2.0644107703471697 1103 74-75 2.2269759251828383 1103 76-77 2.264477160282105 1103 78-79 2.4710439401330397 1103 80-81 2.6046539596809417 1103 82-83 2.906042270717613 1103 84-85 3.0649973227658798 1103 86-87 3.0494307682169897 1103 88-89 3.3900063351871346 1103 90-91 3.7429262633359492 1103 92-93 3.764672973216342 1103 94-95 3.6227864475994735 1103 96-97 3.966737930668369 1103 98-99 4.187622877279274 1103 100-101 4.1391800722972505 1104 1 0.1685987342326598 1104 2 0.2239590218077936 1104 3 0.19874310469313627 1104 4 0.07629384896539193 1104 5 0.05054417005488432 1104 6 0.09720523947147797 1104 7 0.08700407574935554 1104 8 0.12390094556818809 1104 9 0.16604204326340977 1104 10-11 0.2558795914782195 1104 12-13 0.2066157926914869 1104 14-15 0.32824265387086626 1104 16-17 0.2676724760647531 1104 18-19 0.30174102860460295 1104 20-21 0.35341233381949166 1104 22-23 0.33185987279126294 1104 24-25 0.3429307729841682 1104 26-27 0.4158997231961763 1104 28-29 0.3610368932037602 1104 30-31 0.3525642177356616 1104 32-33 0.4964420601647461 1104 34-35 0.5030539988722751 1104 36-37 0.5845734130800864 1104 38-39 0.624887380270799 1104 40-41 0.6419455748927732 1104 42-43 0.638027052334607 1104 44-45 0.7201718424683676 1104 46-47 0.9045018060821164 1104 48-49 0.9789346025491241 1104 50-51 1.1147875584168432 1104 52-53 1.044247495387971 1104 54-55 1.122264724451206 1104 56-57 1.0954772109353215 1104 58-59 1.051109929964852 1104 60-61 1.2101449119034626 1104 62-63 1.461854293615147 1104 64-65 1.5279372224060879 1104 66-67 1.6106555810645773 1104 68-69 1.8236300241629912 1104 70-71 1.6775283633256919 1104 72-73 1.9219548935223578 1104 74-75 1.953616117426602 1104 76-77 1.9775141954781255 1104 78-79 1.861001238281748 1104 80-81 2.12568799999994 1104 82-83 2.0639715493685493 1104 84-85 2.4550800546902103 1104 86-87 2.8105062210989367 1104 88-89 2.8597255343992707 1104 90-91 3.0428011402322177 1104 92-93 3.331439857456889 1104 94-95 3.2073237639535535 1104 96-97 3.74850257807131 1104 98-99 4.0693636806146465 1104 100-101 3.871903996828838 1105 1 0.2644472388030863 1105 2 0.24674959130818763 1105 3 0.15079173473775853 1105 4 0.1316871871066212 1105 5 -0.017509455504324478 1105 6 0.10132272787684116 1105 7 0.17567592555557354 1105 8 0.18852308648625637 1105 9 0.17350893038940995 1105 10-11 0.25877678472607357 1105 12-13 0.27347330912333234 1105 14-15 0.4300944242882707 1105 16-17 0.347940779759206 1105 18-19 0.33163422895874106 1105 20-21 0.2305238475425 1105 22-23 0.3652871651871763 1105 24-25 0.4032074904854639 1105 26-27 0.29613343353841515 1105 28-29 0.4629319053114287 1105 30-31 0.4376367465097033 1105 32-33 0.5546724929334417 1105 34-35 0.48322365288012037 1105 36-37 0.5558096270428976 1105 38-39 0.6966414684427988 1105 40-41 0.7000184320105092 1105 42-43 0.675027281002528 1105 44-45 0.7197797356159512 1105 46-47 0.7771110536473884 1105 48-49 0.948622910582408 1105 50-51 0.9740512477099159 1105 52-53 1.0934660667411649 1105 54-55 1.2724115996161132 1105 56-57 1.2422248494992871 1105 58-59 1.301706518708528 1105 60-61 1.4890266964513756 1105 62-63 1.5121868881751936 1105 64-65 1.6997086072541947 1105 66-67 1.6888028432059627 1105 68-69 1.901157977350568 1105 70-71 2.0478436025768296 1105 72-73 2.1646521782373718 1105 74-75 2.0280379980150407 1105 76-77 2.0235152919114903 1105 78-79 2.2070344361733127 1105 80-81 2.3763498177340168 1105 82-83 2.274524867105562 1105 84-85 2.628744546079986 1105 86-87 2.709485570626647 1105 88-89 2.694526886471646 1105 90-91 3.0081652792388596 1105 92-93 3.1184159000946607 1105 94-95 3.3561206188901416 1105 96-97 3.7538491565542422 1105 98-99 3.7565254426475647 1105 100-101 3.683459642288021 1106 1 0.09803048929870783 1106 2 0.1405474335964172 1106 3 0.10172232516003277 1106 4 0.16668682205907714 1106 5 0.055429851129993324 1106 6 0.052242086067103344 1106 7 0.11893349748397952 1106 8 0.17485346012645664 1106 9 0.2869572817785553 1106 10-11 0.18723097687585977 1106 12-13 0.14113936363335 1106 14-15 0.05723837657374986 1106 16-17 0.11764141269547679 1106 18-19 0.11164394483943596 1106 20-21 0.02454787890289367 1106 22-23 0.028187524056988877 1106 24-25 0.0240452465101626 1106 26-27 2.1887712733814624E-4 1106 28-29 0.10044671781739822 1106 30-31 0.2512189018627069 1106 32-33 0.36626864926365243 1106 34-35 0.27805500091644575 1106 36-37 0.43515860687542585 1106 38-39 0.37542490539347284 1106 40-41 0.28155180016376136 1106 42-43 0.2652880934298665 1106 44-45 0.3190614343787175 1106 46-47 0.5674277464289972 1106 48-49 0.6706736072615982 1106 50-51 0.6211204775719281 1106 52-53 0.8415790517629844 1106 54-55 0.9551108734523126 1106 56-57 0.9526164893489977 1106 58-59 1.0206585233811296 1106 60-61 1.1738558822830356 1106 62-63 1.0493971667907047 1106 64-65 1.0753804351314713 1106 66-67 1.3958058590602604 1106 68-69 1.2430313170862668 1106 70-71 1.3080424692446222 1106 72-73 1.4659202972316372 1106 74-75 1.6558586922793026 1106 76-77 1.5732502112359832 1106 78-79 1.6403704371617494 1106 80-81 1.5868172751498157 1106 82-83 2.1665515268082416 1106 84-85 2.362728085243969 1106 86-87 2.430087309376166 1106 88-89 2.507345250047763 1106 90-91 2.3778489234242137 1106 92-93 2.1870581759695114 1106 94-95 2.524469312300752 1106 96-97 2.863661072829185 1106 98-99 3.0251119785710507 1106 100-101 2.8452753887540787 1107 1 0.16005726683297894 1107 2 0.1804965295483214 1107 3 0.1877217626535952 1107 4 0.1162429432151697 1107 5 0.04319394372635088 1107 6 0.07249371023483064 1107 7 0.17970647044190002 1107 8 0.1538193393769305 1107 9 0.15401988428978086 1107 10-11 0.2530424898789434 1107 12-13 0.23648803408533325 1107 14-15 0.3164984996011455 1107 16-17 0.28028444109648376 1107 18-19 0.18730593416294994 1107 20-21 0.27974653835536856 1107 22-23 0.2754874235298459 1107 24-25 0.31089881661600316 1107 26-27 0.3595414467235898 1107 28-29 0.3922163018132565 1107 30-31 0.3083171344229214 1107 32-33 0.3081042303021704 1107 34-35 0.41367857550563514 1107 36-37 0.47285767706899406 1107 38-39 0.4328642619083851 1107 40-41 0.5225158443065965 1107 42-43 0.4164707492956623 1107 44-45 0.41107022284566597 1107 46-47 0.517064275374409 1107 48-49 0.5447090618754018 1107 50-51 0.3628847706656231 1107 52-53 0.5172603250325096 1107 54-55 0.6102342071662221 1107 56-57 0.6477522706063965 1107 58-59 0.7854250135915706 1107 60-61 0.786524251320678 1107 62-63 1.0138927767342594 1107 64-65 1.0831667634403033 1107 66-67 1.2720349495541825 1107 68-69 1.5286234247310695 1107 70-71 1.3560462675554312 1107 72-73 1.575857934203352 1107 74-75 1.5180714171774525 1107 76-77 1.6200263113955486 1107 78-79 1.4398960660133149 1107 80-81 1.5931705347632494 1107 82-83 1.8365513458036915 1107 84-85 1.7702727028649008 1107 86-87 1.9430472349693737 1107 88-89 2.162260428532168 1107 90-91 2.436017171328043 1107 92-93 2.7285879289400725 1107 94-95 2.8336407430999273 1107 96-97 3.232919051648871 1107 98-99 3.1872394920976603 1107 100-101 2.8005425514660303 1108 1 0.13810426193381886 1108 2 0.16611208788810217 1108 3 0.09202886096112195 1108 4 0.07330399738017945 1108 5 -0.025484283695462295 1108 6 0.060628132851213934 1108 7 0.03584319137684133 1108 8 0.07877756990379225 1108 9 0.08236610334537176 1108 10-11 0.10984919098654444 1108 12-13 0.17796941937363187 1108 14-15 0.2983495456335348 1108 16-17 0.23102022224548335 1108 18-19 0.16570315441629901 1108 20-21 0.32132466631396284 1108 22-23 0.37353486842788186 1108 24-25 0.4077020644419207 1108 26-27 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54-55 0.5466596452538539 2316 56-57 0.44595620044898254 2316 58-59 0.6515937198471846 2316 60-61 0.774783621610915 2316 62-63 0.8628161420305531 2316 64-65 1.157887566228851 2316 66-67 1.1224889262291242 2316 68-69 1.340552487444537 2316 70-71 1.5188897071496221 2316 72-73 1.5965424109346067 2316 74-75 1.9434959220137777 2316 76-77 2.130609007705157 2316 78-79 2.1884386036532284 2316 80-81 2.1747393307785075 2316 82-83 2.274516591955095 2316 84-85 2.454331197177954 2316 86-87 2.4882766738697093 2316 88-89 2.8183334100295703 2316 90-91 2.9392899178430625 2316 92-93 3.2638211370664365 2316 94-95 3.604616966980636 2316 96-97 3.8489222175723476 2316 98-99 4.331715029198218 2316 100-101 4.795895395731911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 40.0 6 81.0 7 191.0 8 439.0 9 882.0 10 1516.0 11 2560.0 12 3956.0 13 6296.0 14 8225.0 15 11518.0 16 15389.0 17 22001.0 18 22752.0 19 17843.0 20 16139.0 21 16842.0 22 18314.0 23 20224.0 24 22935.0 25 25408.0 26 28975.0 27 33604.0 28 39658.0 29 47740.0 30 57196.0 31 68758.0 32 83158.0 33 103699.0 34 135507.0 35 186754.0 36 253466.0 37 306348.0 38 158341.0 39 6883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03064595310084 18.726457512261593 14.892100276660075 22.35079625797749 2 15.815348505862637 20.2204984014124 39.76932616119866 24.194826931526304 3 19.618719889883003 24.361894930029823 30.004129387474194 26.015255792612983 4 12.904178583861588 16.875149472737608 36.57331920953368 33.64735273386712 5 13.503964813358063 37.588382895892785 33.77194140918826 15.13571088156089 6 31.840702432533956 36.15531742231118 16.878224189991712 15.125755955163148 7 28.52254114343655 33.50316805608446 19.839349045912726 18.134941754566267 8 27.015857540217358 34.17406016115619 19.030654087689616 19.77942821093683 9 26.570780016241734 16.769248062691265 20.083835343668703 36.576136577398294 10-11 25.41768559853549 26.498718783980323 26.33234422386067 21.751251393623512 12-13 26.60940575434604 23.987902430295886 27.158223869845887 22.244467945512188 14-15 23.003333226278578 25.22137394073143 26.22825259110742 25.547040241882573 16-17 21.85752862305656 28.556587009303158 26.993577573092846 22.592306794547433 18-19 21.80627944961621 26.59673123597774 29.927634808700798 21.669354505705254 20-21 22.591784159260772 25.461444229825148 30.213987162605466 21.73278444830861 22-23 22.24782941081673 25.5473429029089 30.25912295522441 21.945704731049965 24-25 22.228610492834846 25.837683888998175 30.24003711760465 21.693668500562328 26-27 21.684358499937485 26.03765068360372 30.350278798362424 21.927712018096372 28-29 21.630384663029865 26.577088600044963 30.149094775574998 21.643431961350174 30-31 21.784199406304914 25.94052381891841 29.999151208724655 22.27612556605202 32-33 21.536902921218406 26.086022701725568 30.07749234940837 22.299582027647656 34-35 22.371417198365556 26.13274232736133 29.78250819630759 21.713332277965527 36-37 22.392341341928343 25.668404881147133 29.97698789462893 21.96226588229559 38-39 21.841749436847323 26.17192395323861 29.853358053262557 22.132968556651512 40-41 22.203355173670687 26.0259543162508 29.889620596431342 21.881069913647167 42-43 21.973369366971866 26.064233096040507 29.469830622016158 22.49256691497147 44-45 21.975589528766985 26.20062216247342 29.613339328605935 22.21044898015366 46-47 22.520898617617235 26.604129484256067 28.87771441155824 21.997257486568454 48-49 22.2678957317269 26.667100620764643 28.726050321852476 22.33895332565598 50-51 22.121507912111106 26.95385443000225 28.782282743843968 22.142354914042677 52-53 22.6438299756374 26.849906174153617 28.357629027744004 22.14863482246498 54-55 22.07663094096542 26.834020013121858 28.546284358841422 22.543064687071304 56-57 22.323554646099918 27.021298925934932 28.250927935326693 22.404218492638456 58-59 22.47648045256633 26.576113637093556 28.333885812339133 22.613520098000983 60-61 22.04024188257317 26.8288871199365 28.79131548883495 22.339555508655376 62-63 21.97133494221337 26.921623301843944 28.77359410342407 22.333447652518615 64-65 21.751194042861666 27.08573250687062 28.4959303899393 22.667143060328414 66-67 21.801754991166547 27.058469978731463 28.27118086112767 22.86859416897432 68-69 22.076632415433156 26.766029941464257 28.473582924871625 22.68375471823096 70-71 22.167933353826673 26.918927816037108 28.029353266928798 22.88378556320742 72-73 22.045043314187726 26.89267659026585 28.219716629723195 22.842563465823225 74-75 21.985471905008787 27.488937038039612 27.787046298123023 22.738544758828578 76-77 22.023007978637988 26.933466234165454 28.162865840509827 22.880659946686734 78-79 22.12939364186514 27.17967305477686 28.02516666131393 22.665766642044073 80-81 22.344650273319626 27.291157737957832 27.760860881556525 22.603331107166014 82-83 22.21703185622973 27.233133461228537 27.97888295294546 22.57095172959627 84-85 22.124160018169675 27.36959257888079 27.76645200105875 22.73979540189078 86-87 22.32566449031654 27.29374188456933 27.586958416877344 22.79363520823679 88-89 22.090538500713443 27.483230637235785 27.245655106282427 23.18057575576834 90-91 22.069088330321858 27.24530764790376 27.64376292040329 23.041841101371094 92-93 22.25089123083911 27.0184887386044 27.653275646113684 23.077344384442803 94-95 21.952128731850987 26.89077630300251 27.802854405586423 23.354240559560075 96-97 22.072090543166663 26.66655005335059 27.89781461866442 23.36354478481832 98-99 21.92608519111568 26.953825754621324 27.320125070541433 23.799963983721558 100-101 22.226087026340057 26.100933899805923 27.707185362250353 23.965793711603663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1403.0 1 958.0 2 713.5 3 903.0 4 726.0 5 653.5 6 1262.5 7 2763.5 8 4461.0 9 5093.0 10 4079.0 11 2884.5 12 2684.5 13 2823.5 14 2956.0 15 3075.0 16 3187.0 17 3263.5 18 3415.5 19 3678.0 20 4049.5 21 4568.5 22 5302.5 23 6251.0 24 7479.0 25 9071.5 26 11111.5 27 13580.5 28 15786.0 29 17609.5 30 19850.0 31 23182.0 32 27650.0 33 30421.0 34 31409.5 35 32999.5 36 36126.0 37 39442.0 38 41753.5 39 43342.5 40 45421.5 41 48482.5 42 50592.0 43 52575.5 44 54598.0 45 57550.0 46 59387.5 47 62837.0 48 68868.5 49 68269.5 50 64166.0 51 61897.0 52 60316.5 53 60482.0 54 61021.5 55 57876.5 56 52681.5 57 48052.0 58 44346.5 59 39396.5 60 32449.5 61 27258.5 62 23248.5 63 17722.5 64 12762.5 65 9875.0 66 7663.0 67 6164.0 68 4682.5 69 3262.0 70 2384.0 71 1688.0 72 1227.0 73 998.5 74 749.0 75 554.5 76 384.5 77 231.5 78 127.0 79 65.5 80 31.0 81 18.5 82 9.5 83 2.5 84 4.0 85 4.0 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013878884367099931 3 0.0032116426634611417 4 0.0015484705698830504 5 0.11963368921392752 6 5.735076184752038E-5 7 0.0 8 3.441045710851223E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.867538092376019E-5 18-19 0.0 20-21 0.0 22-23 2.8675380923760195E-4 24-25 5.161568566276834E-4 26-27 0.005849777708447079 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010781943227333831 36-37 4.8748147570392325E-4 38-39 0.007082819088168767 40-41 0.009720954133154705 42-43 0.005735076184752039 44-45 0.0034410457108512227 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.867538092376019E-5 68-69 0.001175690617874168 70-71 0.0 72-73 5.735076184752038E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.602614277128058E-5 82-83 0.002351381235748336 84-85 0.005247594709048115 86-87 0.003670448758241304 88-89 0.0 90-91 0.0027241611877572183 92-93 0.0 94-95 2.867538092376019E-5 96-97 8.602614277128058E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1743656.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.41077533971271 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2731354219062 44.154482574200586 2 12.539343533132596 14.147081819100443 3 3.9443565902474442 6.6751264041649145 4 1.6853226598564812 3.80281431760364 5 0.9077621073224594 2.5603782149035714 6 0.5743760824724884 1.94406000893159 7 0.3775104758633282 1.4906961049619967 8 0.26162059090362805 1.1806576302165956 9 0.2002538626782324 1.01668280926063 >10 1.1213282363484787 11.560244039517018 >50 0.0740291892199009 2.839417060709083 >100 0.034558503325126604 3.659672402636807 >500 0.00426849781579888 1.7325361379628736 >1k 0.001829356206770949 1.6558275558121678 >5k 2.032618007523276E-4 0.9214202782706586 >10k+ 1.016309003761638E-4 0.6589026417474291 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11493 0.6591323059135518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9864 0.565707914863941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6208 0.35603352954940654 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3517 0.20170262941772918 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.14383571071358112 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2028 0.11630734502677134 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1830 0.1049518941809623 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1747 0.10019178094761812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 2 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 3 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 4 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 5 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 6 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 7 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 8 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 9 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 10-11 0.0 0.0 0.0 5.7350761847520384E-5 5.7350761847520384E-5 12-13 0.0 0.0 0.0 5.7350761847520384E-5 1.1470152369504077E-4 14-15 0.0 0.0 0.0 5.7350761847520384E-5 1.4337690461880095E-4 16-17 0.0 0.0 0.0 5.7350761847520384E-5 1.7205228554256115E-4 18-19 0.0 0.0 0.0 5.7350761847520384E-5 2.2940304739008154E-4 20-21 0.0 0.0 0.0 1.1470152369504077E-4 2.867538092376019E-4 22-23 0.0 0.0 0.0 2.580784283138417E-4 2.867538092376019E-4 24-25 0.0 0.0 0.0 6.882091421702446E-4 2.867538092376019E-4 26-27 0.0 0.0 0.0 0.0013764182843404892 3.154291901613621E-4 28-29 0.0 0.0 0.0 0.004731437852420432 3.727799520088825E-4 30-31 0.0 0.0 0.0 0.014653119652041458 4.0145533293264266E-4 32-33 0.0 0.0 0.0 0.03584422615470024 4.0145533293264266E-4 34-35 0.0 0.0 0.0 0.06853416040778686 4.5880609478016307E-4 36-37 0.0 0.0 0.0 0.11974839073762256 4.5880609478016307E-4 38-39 0.0 0.0 0.0 0.19178094761810816 4.5880609478016307E-4 40-41 0.0 0.0 0.0 0.26934785301687947 4.5880609478016307E-4 42-43 0.0 0.0 0.0 0.3472588629867359 5.161568566276834E-4 44-45 0.0 0.0 0.0 0.43985166798955755 5.735076184752038E-4 46-47 0.0 0.0 0.0 0.5479865294530573 5.735076184752038E-4 48-49 0.0 0.0 0.0 0.6645232775272187 5.735076184752038E-4 50-51 0.0 0.0 0.0 0.7891464830218804 5.735076184752038E-4 52-53 0.0 0.0 0.0 0.9034752267649123 6.308583803227243E-4 54-55 0.0 0.0 0.0 1.026205857118606 6.308583803227243E-4 56-57 0.0 0.0 0.0 1.1694107094518644 6.308583803227243E-4 58-59 0.0 0.0 0.0 1.3256055093435861 6.308583803227243E-4 60-61 0.0 0.0 0.0 1.4935285400331257 6.308583803227243E-4 62-63 0.0 0.0 0.0 1.6545694793009629 6.308583803227243E-4 64-65 0.0 0.0 0.0 1.8156677693306478 6.308583803227243E-4 66-67 0.0 0.0 0.0 2.0010254316218337 6.308583803227243E-4 68-69 0.0 0.0 0.0 2.210040283175122 6.308583803227243E-4 70-71 0.0 0.0 0.0 2.423213064962355 6.308583803227243E-4 72-73 0.0 0.0 0.0 2.6427517813146633 6.308583803227243E-4 74-75 0.0 0.0 0.0 2.856440719958524 6.308583803227243E-4 76-77 0.0 0.0 0.0 3.0758934101680606 6.308583803227243E-4 78-79 0.0 0.0 0.0 3.3289536468202447 6.308583803227243E-4 80-81 0.0 0.0 0.0 3.599247787407608 6.308583803227243E-4 82-83 0.0 0.0 0.0 3.8752770041797233 6.308583803227243E-4 84-85 0.0 0.0 0.0 4.150876090237983 6.308583803227243E-4 86-87 0.0 0.0 0.0 4.427048683914717 6.595337612464844E-4 88-89 0.0 0.0 0.0 4.7298033557077765 6.882091421702446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9170 0.0 31.430387 1 GTATCAA 12880 0.0 28.543686 1 GTACATG 8205 0.0 24.461344 1 GTGTAAG 700 0.0 24.459688 1 TAAGGTG 955 0.0 24.397165 5 TATCAAC 15130 0.0 24.236053 2 ATCAACG 15135 0.0 24.16173 3 TCAACGC 15210 0.0 24.041208 4 CAACGCA 15450 0.0 23.667072 5 TACATGG 8425 0.0 23.48388 2 GAGTACT 9090 0.0 23.172108 12-13 AACGCAG 15805 0.0 23.13833 6 AGGTAAG 880 0.0 22.158873 2 GTACTTT 9550 0.0 21.956503 14-15 ACATGGG 8565 0.0 21.764206 3 GTAAGGT 1000 0.0 21.397926 4 ACGCAGA 17485 0.0 20.915146 7 CGCAGAG 17530 0.0 20.753084 8 GTGTAGC 870 0.0 20.226881 1 AGTACTT 9440 0.0 19.696796 12-13 >>END_MODULE