FastQCFastQC Report
Thu 26 May 2016
SRR938034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741380
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119040.6835957688729628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97790.5615661142312419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62120.3567285715926449No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36140.20753655147067268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.13719004467721002No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19210.11031480779611573No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17870.10261976133870837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93050.030.9205041
GTATCAA133700.029.2993091
TAAGGTG8050.026.5688955
GTAAGGT7750.025.1442724
TATCAAC157700.024.7726272
ATCAACG158800.024.512653
CAACGCA160400.024.2681375
TCAACGC161000.024.2072184
AACGCAG163650.023.7841326
GAGTACT92850.023.48317312-13
GGTAAGG8200.023.1847823
TACCGTA1050.002194071222.623667
GTACATG83300.022.4536361
AGGTAAG8150.022.1593972
GTACTTT98200.022.08444414-15
TACATGG82800.021.8688812
ACGCAGA179550.021.5387827
CGCAGAG180900.021.351788
AGTACTT95600.020.74553112-13
TATAACG1150.00371221620.663512
Produced by FastQC (version 0.11.5)