##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938034_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1741380 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894904041622162 33.0 31.0 34.0 30.0 34.0 2 32.068255062077206 34.0 31.0 34.0 30.0 34.0 3 32.14155956769918 34.0 31.0 34.0 30.0 34.0 4 35.660334906798056 37.0 35.0 37.0 35.0 37.0 5 35.68154222513179 37.0 35.0 37.0 35.0 37.0 6 35.71575934029333 37.0 35.0 37.0 35.0 37.0 7 35.6589251053762 37.0 35.0 37.0 33.0 37.0 8 35.637833212739324 37.0 35.0 37.0 33.0 37.0 9 37.274541455627144 39.0 37.0 39.0 34.0 39.0 10-11 37.1871593219171 39.0 37.0 39.0 34.0 39.0 12-13 37.08259828411949 39.0 37.0 39.0 33.0 39.0 14-15 38.247593288081866 40.0 38.0 41.0 33.0 41.0 16-17 38.105687156163505 40.0 38.0 41.0 33.0 41.0 18-19 38.27986424559832 40.0 38.0 41.0 33.5 41.0 20-21 38.26904839839668 40.0 38.0 41.0 34.0 41.0 22-23 38.17821756308216 40.0 38.0 41.0 33.5 41.0 24-25 38.066831191353984 40.0 38.0 41.0 33.0 41.0 26-27 37.88438537252064 40.0 38.0 41.0 33.0 41.0 28-29 37.67658638551035 40.0 38.0 41.0 32.5 41.0 30-31 37.43876695494378 40.0 37.0 41.0 32.0 41.0 32-33 37.15287588004915 40.0 36.5 41.0 31.0 41.0 34-35 36.90089411845777 39.5 36.0 41.0 31.0 41.0 36-37 36.63179834384224 39.0 35.5 41.0 30.0 41.0 38-39 36.33006207720314 39.0 35.0 41.0 30.0 41.0 40-41 36.10698727446048 39.0 35.0 40.0 30.0 41.0 42-43 35.89089831053532 39.0 35.0 40.5 28.5 41.0 44-45 35.583552699583095 39.0 35.0 40.0 27.0 41.0 46-47 35.12895318655319 38.5 35.0 40.0 25.0 41.0 48-49 34.669185358738474 38.0 34.0 40.0 23.5 41.0 50-51 33.94221680506266 37.5 33.5 39.5 23.0 40.5 52-53 34.05549047307308 38.0 33.5 40.0 23.0 40.5 54-55 34.14360277481078 38.0 34.0 40.0 22.5 41.0 56-57 33.83140784894739 38.0 33.5 40.0 20.5 41.0 58-59 33.514636667470626 37.5 33.0 40.0 18.5 41.0 60-61 33.078249434356664 37.0 33.0 40.0 16.5 41.0 62-63 32.54068066705716 36.0 32.0 39.5 10.5 41.0 64-65 32.01670169635577 36.0 31.0 39.0 7.5 40.5 66-67 31.474142346874316 35.0 31.0 39.0 4.5 40.0 68-69 30.87420695080913 35.0 30.0 38.0 2.0 40.0 70-71 30.30595476001792 35.0 29.5 37.5 2.0 39.5 72-73 29.663479252087427 34.0 29.0 37.0 2.0 39.0 74-75 28.9761927322009 34.0 28.0 36.0 2.0 39.0 76-77 28.305356958274473 34.0 26.5 35.5 2.0 38.5 78-79 27.577274919891124 33.5 26.0 35.0 2.0 37.0 80-81 26.905487888915687 33.0 24.5 35.0 2.0 37.0 82-83 26.165448667149043 32.0 23.0 35.0 2.0 36.0 84-85 25.500339386004207 32.0 20.0 35.0 2.0 36.0 86-87 24.814591875409157 31.5 17.5 34.5 2.0 35.0 88-89 23.98853208374967 31.0 8.5 34.0 2.0 35.0 90-91 23.140083726699515 30.5 2.0 34.0 2.0 35.0 92-93 22.242251834751748 29.5 2.0 34.0 2.0 35.0 94-95 21.282680402898848 29.0 2.0 34.0 2.0 35.0 96-97 20.071486694460717 28.0 2.0 34.0 2.0 35.0 98-99 18.5977173276367 25.5 2.0 33.5 2.0 35.0 100-101 14.96138953014276 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2972017663505717 1101 2 0.32040226645577263 1101 3 0.3075379250147421 1101 4 0.2287131423225759 1101 5 0.22569185402002745 1101 6 0.25323913609116744 1101 7 0.20757493028673224 1101 8 0.2147372316957359 1101 9 0.25506986998514947 1101 10-11 0.27732079106542074 1101 12-13 0.3530969889708402 1101 14-15 0.4894625406479065 1101 16-17 0.5338689412938464 1101 18-19 0.44134781318135907 1101 20-21 0.43213402992966365 1101 22-23 0.4834997268095549 1101 24-25 0.5107449297768767 1101 26-27 0.5794090237784175 1101 28-29 0.5364309079344025 1101 30-31 0.6492632433591368 1101 32-33 0.37284784022928363 1101 34-35 0.5605767391748131 1101 36-37 0.4860077295478362 1101 38-39 0.5541943410831465 1101 40-41 0.697416521676594 1101 42-43 0.7805055568482047 1101 44-45 0.6745381916358824 1101 46-47 0.8412990455956475 1101 48-49 0.8685946794551 1101 50-51 0.7579486406884826 1101 52-53 0.8984022564999279 1101 54-55 1.0941267152246397 1101 56-57 0.9193735093834121 1101 58-59 1.0358801595653517 1101 60-61 1.1330833183793132 1101 62-63 1.0924671216106177 1101 64-65 1.0895754073094537 1101 66-67 1.1693874425845223 1101 68-69 1.4010571711941573 1101 70-71 1.6888591045616188 1101 72-73 1.7893323593895154 1101 74-75 1.9555668727820503 1101 76-77 1.9444414493056854 1101 78-79 2.128228653215235 1101 80-81 2.54507430009523 1101 82-83 2.549815004665085 1101 84-85 2.6974590100788483 1101 86-87 2.911170571676884 1101 88-89 3.318339622004391 1101 90-91 3.7068725321125697 1101 92-93 3.6808535960655675 1101 94-95 3.953086750212936 1101 96-97 4.1968337694060835 1101 98-99 4.425813127469336 1101 100-101 4.453546683653819 1102 1 0.2989506732837377 1102 2 0.286893447562818 1102 3 0.2594318801816513 1102 4 0.26662119582088195 1102 5 0.34197076700100126 1102 6 0.3698083438283959 1102 7 0.13835945979705855 1102 8 0.34860237550466877 1102 9 0.47544443746777176 1102 10-11 0.4373755187653714 1102 12-13 0.4819049760424079 1102 14-15 0.6437982237031434 1102 16-17 0.6123924831278202 1102 18-19 0.4320792013018817 1102 20-21 0.4816376139991405 1102 22-23 0.6111199641612117 1102 24-25 0.520580449243873 1102 26-27 0.519136376722507 1102 28-29 0.4591932687604725 1102 30-31 0.6735686873772622 1102 32-33 0.6857707158129926 1102 34-35 0.7640005913307064 1102 36-37 0.8388637818167552 1102 38-39 0.8352980163654635 1102 40-41 1.147792972902515 1102 42-43 1.0919555434637047 1102 44-45 1.0785638502024497 1102 46-47 1.040864793193876 1102 48-49 1.0896513602994133 1102 50-51 1.2076929473222293 1102 52-53 1.1502952202230006 1102 54-55 1.2435973833586473 1102 56-57 1.2495633708786968 1102 58-59 1.2119576111581054 1102 60-61 1.2876385071006453 1102 62-63 1.3428261234249632 1102 64-65 1.5680943885017626 1102 66-67 1.6476000676303784 1102 68-69 1.6798442388007082 1102 70-71 1.6707367009051772 1102 72-73 1.9727849634167605 1102 74-75 1.9916859650160674 1102 76-77 1.9814367796051968 1102 78-79 2.509158429248071 1102 80-81 2.426073675485611 1102 82-83 2.631438196519383 1102 84-85 2.6270310037732685 1102 86-87 2.6692253588634607 1102 88-89 3.2798485995283606 1102 90-91 3.423571420704029 1102 92-93 3.74500278853456 1102 94-95 3.84649186408026 1102 96-97 3.959548937505385 1102 98-99 4.47190257553255 1102 100-101 4.24791825697605 1103 1 0.5139945046259093 1103 2 0.4494563691861231 1103 3 0.3612007254854106 1103 4 0.32085876103400324 1103 5 0.3587908142294296 1103 6 0.36132981159485666 1103 7 0.3671796657764901 1103 8 0.3975756439138749 1103 9 0.31103461796199383 1103 10-11 0.4971320438570075 1103 12-13 0.5593095577098879 1103 14-15 0.663612097596257 1103 16-17 0.819939109149189 1103 18-19 0.8223491819588347 1103 20-21 0.8696536610801573 1103 22-23 0.8034997268095552 1103 24-25 0.8597817426866143 1103 26-27 0.8143565539755642 1103 28-29 0.9785011238170256 1103 30-31 1.0237252177605924 1103 32-33 1.0110460968390313 1103 34-35 1.0055336346914672 1103 36-37 1.143857067972064 1103 38-39 1.19457351645476 1103 40-41 1.2065421850533866 1103 42-43 1.4065310593336093 1103 44-45 1.3597527535070242 1103 46-47 1.6051561164118695 1103 48-49 1.7028006684568524 1103 50-51 1.6618739102415887 1103 52-53 1.7083638346763337 1103 54-55 1.9515675816367661 1103 56-57 1.9330214213974344 1103 58-59 1.9370148040434145 1103 60-61 1.8461479390838704 1103 62-63 2.005371091065264 1103 64-65 2.323863522959144 1103 66-67 2.4105878844355004 1103 68-69 2.428782118964488 1103 70-71 2.573199089673164 1103 72-73 2.620051327763548 1103 74-75 2.7544977855404937 1103 76-77 2.9274052127233077 1103 78-79 3.3781486877948765 1103 80-81 3.374166584512782 1103 82-83 3.276548429250248 1103 84-85 3.4448864288527155 1103 86-87 3.6646220521177746 1103 88-89 3.9572580476701695 1103 90-91 4.117467349969111 1103 92-93 4.428123965635482 1103 94-95 4.680963464186473 1103 96-97 5.236849858544712 1103 98-99 4.967979157774547 1103 100-101 4.786518611708956 1104 1 0.37824014106839954 1104 2 0.3510590438394132 1104 3 0.30242631227333305 1104 4 0.2740787949794239 1104 5 0.24001769872962342 1104 6 0.3095331631075453 1104 7 0.3306789469121938 1104 8 0.3632366404886014 1104 9 0.4687023899607823 1104 10-11 0.47081751255311133 1104 12-13 0.3617063981220099 1104 14-15 0.5849220698320465 1104 16-17 0.56944563698341 1104 18-19 0.5234412239090886 1104 20-21 0.6132696850946644 1104 22-23 0.5574500654100021 1104 24-25 0.4946200238325602 1104 26-27 0.5556025455814151 1104 28-29 0.7169305675424198 1104 30-31 0.6642802629585489 1104 32-33 0.6759264170324641 1104 34-35 0.6369739587097243 1104 36-37 0.7685164185012212 1104 38-39 0.8959041837862216 1104 40-41 0.9362466481590914 1104 42-43 0.872511361427371 1104 44-45 0.9161921383914091 1104 46-47 0.8775561669543492 1104 48-49 0.9612456164288403 1104 50-51 0.7872073027688131 1104 52-53 0.7260694964583649 1104 54-55 1.0616579775414579 1104 56-57 1.1760889058504986 1104 58-59 1.0402819654344242 1104 60-61 1.059453844016204 1104 62-63 1.1022231143011467 1104 64-65 1.279885164018765 1104 66-67 1.3481256632301921 1104 68-69 1.4541502594452602 1104 70-71 1.689841225024555 1104 72-73 1.586734272750796 1104 74-75 1.8780762783501466 1104 76-77 2.088535827463268 1104 78-79 2.2755005010062703 1104 80-81 2.336868882488009 1104 82-83 2.3963315405495678 1104 84-85 2.4931856573969178 1104 86-87 2.4234601556820436 1104 88-89 2.687176377172978 1104 90-91 2.886584273844626 1104 92-93 2.9445378914541536 1104 94-95 3.112452367039051 1104 96-97 3.516150835585453 1104 98-99 3.6928402513361895 1104 100-101 3.8774681785121103 1105 1 0.2898983955640553 1105 2 0.27885598502184905 1105 3 0.1234055904329665 1105 4 0.1810816103090218 1105 5 0.2054956706777773 1105 6 0.21950095167883177 1105 7 0.2773056240623717 1105 8 0.24628886358444646 1105 9 0.3276505726782091 1105 10-11 0.22251875789260112 1105 12-13 0.22564291370778022 1105 14-15 0.27543364819204896 1105 16-17 0.33905085675666413 1105 18-19 0.28433604218083275 1105 20-21 0.30893445796604624 1105 22-23 0.23467950209045085 1105 24-25 0.3524490496270616 1105 26-27 0.4032684851659596 1105 28-29 0.4055043875135027 1105 30-31 0.28727465766268523 1105 32-33 0.3324492313475247 1105 34-35 0.3796395178443319 1105 36-37 0.24069918665502144 1105 38-39 0.29921663464476467 1105 40-41 0.37182886339230237 1105 42-43 0.4890609340552672 1105 44-45 0.5990896451761003 1105 46-47 0.47562221306488084 1105 48-49 0.5101873314971925 1105 50-51 0.5124501566506723 1105 52-53 0.5131284915632435 1105 54-55 0.4861215431183439 1105 56-57 0.46514843358529845 1105 58-59 0.5931835303518653 1105 60-61 0.5540704772878158 1105 62-63 0.45718354750682266 1105 64-65 0.549338203813825 1105 66-67 0.6163358998700588 1105 68-69 0.629855994094104 1105 70-71 0.939488674741753 1105 72-73 1.0566089779020906 1105 74-75 1.1941249252165065 1105 76-77 1.2836495819969613 1105 78-79 1.4803474333115432 1105 80-81 1.68181587312894 1105 82-83 1.668508601954187 1105 84-85 1.6625125146267372 1105 86-87 1.9055606198309754 1105 88-89 2.223461790722066 1105 90-91 2.419062651606062 1105 92-93 2.471953114524638 1105 94-95 2.604340540118411 1105 96-97 2.7453953888103975 1105 98-99 2.9700176931907514 1105 100-101 3.170556671169429 1106 1 0.12532325116496779 1106 2 0.2089661959645852 1106 3 0.04497824497474312 1106 4 0.1302929448472625 1106 5 0.19863023672218816 1106 6 0.06982956228789305 1106 7 0.2626230492718591 1106 8 0.22818305096832603 1106 9 0.3007866553869718 1106 10-11 0.1740774464834942 1106 12-13 0.3033432081934393 1106 14-15 0.341577276305955 1106 16-17 0.14244786892788852 1106 18-19 0.1646361521389963 1106 20-21 0.20387006292553878 1106 22-23 0.19720051421112572 1106 24-25 0.2269466545612815 1106 26-27 0.261035070522567 1106 28-29 0.28329504991666 1106 30-31 0.2611292601070403 1106 32-33 0.37099557176284037 1106 34-35 0.43004680541653073 1106 36-37 0.47963227647947093 1106 38-39 0.26200211511139315 1106 40-41 0.6091950493606291 1106 42-43 0.4470859842947661 1106 44-45 0.5194925723382937 1106 46-47 0.7210140812161967 1106 48-49 0.6656675453468637 1106 50-51 0.6822855985687468 1106 52-53 0.6375423639864906 1106 54-55 0.23631144213378263 1106 56-57 0.626676346528761 1106 58-59 0.8051152551784 1106 60-61 0.9814522722600785 1106 62-63 0.7227220897395981 1106 64-65 0.87456728332495 1106 66-67 1.148005115375426 1106 68-69 0.8689799308491963 1106 70-71 1.1142109355827436 1106 72-73 1.4149304096332358 1106 74-75 1.4185290025158324 1106 76-77 1.752611678027094 1106 78-79 1.9647415890982494 1106 80-81 2.1285613642122136 1106 82-83 2.3562479505468517 1106 84-85 2.4380964304013126 1106 86-87 2.527357298336053 1106 88-89 2.7966060886960555 1106 90-91 3.19000589044278 1106 92-93 3.305251796290541 1106 94-95 3.459497198906849 1106 96-97 3.4829205085636907 1106 98-99 3.884435799635316 1106 100-101 4.1693822042137505 1107 1 0.23571344478620304 1107 2 0.17486542053869414 1107 3 0.10658675113580784 1107 4 0.016072669149785668 1107 5 0.14319562612455883 1107 6 0.20240685894710708 1107 7 0.13782781217459217 1107 8 0.2016718686683987 1107 9 0.18278548348023094 1107 10-11 0.1407283594159594 1107 12-13 0.21268296277734322 1107 14-15 0.31799051457512917 1107 16-17 0.24689084967697283 1107 18-19 0.2298576810068269 1107 20-21 0.29646138824941914 1107 22-23 0.3064872335993485 1107 24-25 0.42136234784676674 1107 26-27 0.42088165338792294 1107 28-29 0.566036798452842 1107 30-31 0.47594318783375655 1107 32-33 0.46439953314979476 1107 34-35 0.44806059456740854 1107 36-37 0.5290736961119009 1107 38-39 0.7182042390854448 1107 40-41 0.6721379894779105 1107 42-43 0.7500655785755228 1107 44-45 0.7139563822327801 1107 46-47 0.7649456144893705 1107 48-49 0.8453114396699561 1107 50-51 0.7552496244533415 1107 52-53 0.7049590794826059 1107 54-55 1.0109460766827851 1107 56-57 1.1179201929491 1107 58-59 1.0328383344702914 1107 60-61 1.0485097170756745 1107 62-63 1.1432837067998278 1107 64-65 1.5032503424292543 1107 66-67 1.5832615448236425 1107 68-69 1.6013649749240138 1107 70-71 1.799068531805272 1107 72-73 1.9410940830904053 1107 74-75 1.941838826430427 1107 76-77 2.151875814686818 1107 78-79 2.175235714594919 1107 80-81 2.5022606118823028 1107 82-83 2.5631761006877767 1107 84-85 2.486580864143672 1107 86-87 2.7383079969892137 1107 88-89 2.780880511617525 1107 90-91 3.0952001318470153 1107 92-93 3.408697159346392 1107 94-95 3.7561382318955943 1107 96-97 3.9159550199341773 1107 98-99 4.203733339914262 1107 100-101 4.1740548615764475 1108 1 0.21475579903374964 1108 2 0.15811092925668646 1108 3 0.13788291417891685 1108 4 0.15455628646829922 1108 5 0.11382164960370034 1108 6 0.09971720087181524 1108 7 0.1640629523098056 1108 8 0.15943587868220988 1108 9 0.1978122116942771 1108 10-11 0.21488888103203863 1108 12-13 0.037666777525871 1108 14-15 0.12991588803852494 1108 16-17 0.3066399832899833 1108 18-19 0.2536795650703141 1108 20-21 0.23094970380068247 1108 22-23 0.28584026450064215 1108 24-25 0.26487952826571615 1108 26-27 0.20657589544712351 1108 28-29 0.18969512629087149 1108 30-31 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-1.3349606884885503 2316 52-53 -1.3311558269516581 2316 54-55 -1.1130846122309492 2316 56-57 -1.169927286981384 2316 58-59 -1.2011496401415371 2316 60-61 -1.4160687508509184 2316 62-63 -1.5071667211790611 2316 64-65 -1.533148361892085 2316 66-67 -1.7361799770229567 2316 68-69 -1.7400389741521103 2316 70-71 -1.7227487458737833 2316 72-73 -1.8066054822835476 2316 74-75 -1.9365419042900882 2316 76-77 -1.998714853762685 2316 78-79 -2.6944205197905546 2316 80-81 -2.74532140093066 2316 82-83 -2.7593736647776126 2316 84-85 -2.5637576368143975 2316 86-87 -2.309400298586219 2316 88-89 -2.5256832996540943 2316 90-91 -2.926882634178014 2316 92-93 -3.1771797628568983 2316 94-95 -3.2658597529934603 2316 96-97 -3.70571599370059 2316 98-99 -3.685954461181499 2316 100-101 -3.4882000459776172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4770.0 3 1426.0 4 1557.0 5 1620.0 6 2055.0 7 2511.0 8 3110.0 9 4002.0 10 5022.0 11 6455.0 12 8031.0 13 10371.0 14 12011.0 15 14806.0 16 18363.0 17 23497.0 18 22924.0 19 17965.0 20 17354.0 21 18265.0 22 19962.0 23 22136.0 24 24645.0 25 27630.0 26 31671.0 27 36019.0 28 41425.0 29 48662.0 30 57081.0 31 68934.0 32 84031.0 33 105684.0 34 137524.0 35 187405.0 36 239568.0 37 265667.0 38 138848.0 39 8373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76186410464388 18.368060403063996 14.523699364609428 22.3463761276827 2 15.647111272483263 20.03334428723426 40.3518155516285 23.96772888865398 3 19.627027412152177 24.500573432713015 29.795690176258738 26.076708978876074 4 12.643851862748026 16.61964498727829 36.906636205045935 33.82986694492775 5 13.500272433207883 37.54020401453897 33.71355200025749 15.24597155199566 6 31.676344297994795 36.08303355953108 17.121734697455 15.11888744501912 7 28.428279648041276 33.538920280716674 20.027864835439473 18.00493523580258 8 26.83766418795327 34.143511071484376 19.245953324202844 19.772871416359507 9 26.805087959186018 16.810316101529374 20.122890071125717 36.26170586815889 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26.192496722142234 29.762029249637738 21.686490859344566 36-37 22.38505028139021 25.68721634398693 29.971942849247178 21.955790525375676 38-39 21.84706329051392 26.225517368544704 29.81393860680565 22.113480734135727 40-41 22.2298841595493 26.086934044984027 29.866697608001246 21.81648418746543 42-43 21.9591581887105 26.128408474404434 29.44685231195968 22.465581024925378 44-45 21.996926691748218 26.24499567950639 29.545083180629945 22.212994448115452 46-47 22.598236092889458 26.55760672017131 28.89449069106466 21.949666495874567 48-49 22.326605989413554 26.6442453781223 28.69875151142653 22.33039712103762 50-51 22.205534239289783 26.927301690045958 28.755047436788043 22.112116633876216 52-53 22.63804168998747 26.820796002098657 28.441626579772084 22.099535728141788 54-55 22.097844422325743 26.880824944532545 28.559724222062812 22.4616064110789 56-57 22.28144331110724 27.038176255936186 28.340268039881188 22.34011239307538 58-59 22.446047545300075 26.60470341405729 28.360421436651112 22.588827603991525 60-61 21.990736153841073 26.800940456426353 28.924347936713612 22.283975453018964 62-63 21.980943246138253 26.808826527828433 28.962934671173358 22.24729555485996 64-65 21.771146924635854 27.00112831843794 28.63789363710534 22.58983111982087 66-67 21.84610684111896 26.996314655061017 28.36294186665564 22.79463663716438 68-69 22.126643492294594 26.685619684719413 28.61090171855055 22.576835104435435 70-71 22.171712834939797 26.818445636388127 28.23081944389002 22.77902208478206 72-73 22.15429194193842 26.80516287998989 28.38926357596495 22.651281602106735 74-75 22.023408341070162 27.45536451439768 27.98088484413728 22.540342300394887 76-77 22.29577575606862 26.96186734404963 28.043269885154643 22.699087014727105 78-79 22.425006985343842 27.146525089373636 27.93922162162783 22.489246303654692 80-81 22.616280121636688 27.212031108161483 27.68831416474713 22.4833746054547 82-83 22.52775622422308 27.227059726926196 27.870201207139456 22.37498284171127 84-85 22.486806217230917 27.33908581687736 27.68181690262991 22.492291063261813 86-87 22.631881048415465 27.253248768817333 27.49507771001603 22.619792472751175 88-89 22.48715285716091 27.294161371372898 27.32882826474822 22.889857506717966 90-91 22.59665856588959 27.205743174250387 27.3640343305973 22.833563929262723 92-93 22.823371225871075 26.882268543832964 27.487444459250206 22.80691577104575 94-95 22.641506182314515 26.715847911156658 27.63799624534402 23.00464966118481 96-97 22.774546485374746 26.430006051552795 27.707696500984706 23.087750962087757 98-99 22.72948681643065 26.76425699789249 27.038307972177883 23.46794821349898 100-101 23.4953397176599 25.56744217744586 27.30758956158868 23.629628543305557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1416.0 1 995.5 2 765.5 3 954.5 4 757.0 5 673.0 6 1248.5 7 2669.5 8 4420.5 9 5161.5 10 4070.5 11 2784.0 12 2635.0 13 2783.0 14 2911.0 15 3033.5 16 3124.0 17 3247.5 18 3498.0 19 3843.5 20 4145.5 21 4692.0 22 5518.0 23 6374.0 24 7496.5 25 9120.0 26 11432.0 27 13969.5 28 15906.0 29 17715.5 30 19969.5 31 23315.5 32 27713.0 33 30027.0 34 31062.0 35 33011.0 36 36091.0 37 39598.0 38 42015.0 39 43604.0 40 45670.5 41 48529.5 42 50356.5 43 51953.5 44 54077.5 45 57207.0 46 59337.0 47 62230.0 48 67800.0 49 67921.0 50 63888.5 51 61231.5 52 59577.5 53 59704.0 54 60124.0 55 57053.5 56 52095.0 57 47756.5 58 44285.5 59 39538.0 60 32852.5 61 27621.0 62 23219.5 63 17973.0 64 13325.5 65 10383.5 66 8000.5 67 6301.5 68 4933.0 69 3629.5 70 2734.0 71 1914.0 72 1406.0 73 1135.5 74 815.5 75 603.5 76 443.0 77 268.0 78 157.0 79 96.0 80 55.0 81 33.0 82 21.0 83 11.5 84 11.5 85 9.0 86 7.0 87 7.0 88 4.0 89 1.5 90 1.0 91 1.5 92 4.0 93 4.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.456993878418266 2 0.04318414131321136 3 0.056506908314095716 4 0.060182154383305195 5 0.08636828262642272 6 0.08366927379434701 7 0.04795047605921741 8 0.03973859812332747 9 0.04594057586511847 10-11 0.05050592059171462 12-13 0.04660097164317955 14-15 0.0389633509056036 16-17 0.04114552825919673 18-19 0.05027621771238902 20-21 0.05828710562886906 22-23 0.05030493057230472 24-25 0.06822175515970093 26-27 0.0598950257841482 28-29 0.047261367421240624 30-31 0.049271267615339565 32-33 0.048151466078627296 34-35 0.05248710792589785 36-37 0.04740493172081912 38-39 0.02888513707519324 40-41 0.025898999643960537 42-43 0.02546830674522505 44-45 0.014901974296247804 46-47 0.027104939760419895 48-49 0.027277216919914093 50-51 0.015705934373887377 52-53 0.01530395433506759 54-55 0.015447518634646086 56-57 0.014844548576416407 58-59 0.01381088561945124 60-61 0.017830686007649105 62-63 0.015217815755320493 64-65 0.016854448770515337 66-67 0.009130689453192297 68-69 0.013351479860800056 70-71 0.01949603188275965 72-73 0.01708415164984093 74-75 0.014270291378102424 76-77 0.009704946651506277 78-79 0.013466331300462852 80-81 0.016854448770515337 82-83 0.013724747039704142 84-85 0.01257623264307618 86-87 0.003818810368787973 88-89 0.030205928631315392 90-91 0.034484144758754554 92-93 0.018146527466721796 94-95 0.03491483765749004 96-97 0.028971275654940332 98-99 0.026588108281937313 100-101 0.14876132722323673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1741380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.09001438726678 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4423613051087 46.1480791117221 2 12.066944642535308 14.019379757904554 3 3.621144265261897 6.3105696740229655 4 1.6016998662799664 3.7217107310514606 5 0.8130415191366792 2.3614796772047466 6 0.5212296158012352 1.816694152857797 7 0.34271677458238864 1.3935895656374038 8 0.2535520083060297 1.1783071848334121 9 0.19384794047658 1.013456668610391 >10 1.0400726099053899 11.055622166843646 >50 0.06488093459937168 2.5666763326200632 >100 0.032454928730573776 3.5048966820161738 >500 0.003969566738278704 1.6732922190273312 >1k 0.0017863050322254167 1.6281537980427518 >5k 1.9847833691393518E-4 0.9218499233261922 >10k+ 9.923916845696759E-5 0.6862423542789688 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11904 0.6835957688729628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9779 0.5615661142312419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6212 0.3567285715926449 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3614 0.20753655147067268 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2389 0.13719004467721002 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1921 0.11031480779611573 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1787 0.10261976133870837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 5.742571983139809E-5 6 0.0 0.0 0.0 0.0 5.742571983139809E-5 7 0.0 0.0 0.0 0.0 5.742571983139809E-5 8 0.0 0.0 0.0 0.0 5.742571983139809E-5 9 0.0 0.0 0.0 5.742571983139809E-5 5.742571983139809E-5 10-11 0.0 0.0 0.0 5.742571983139809E-5 5.742571983139809E-5 12-13 0.0 0.0 0.0 1.1485143966279618E-4 1.1485143966279618E-4 14-15 0.0 0.0 0.0 1.1485143966279618E-4 1.1485143966279618E-4 16-17 0.0 0.0 0.0 1.435642995784952E-4 1.1485143966279618E-4 18-19 0.0 0.0 0.0 2.8712859915699045E-4 1.1485143966279618E-4 20-21 0.0 0.0 0.0 3.1584145907268945E-4 2.009900194098933E-4 22-23 0.0 0.0 0.0 5.455443383982819E-4 2.2970287932559235E-4 24-25 0.0 0.0 0.0 9.188115173023694E-4 2.2970287932559235E-4 26-27 0.0 0.0 0.0 0.0019811873341832337 2.584157392412914E-4 28-29 0.0 0.0 0.0 0.00568514626330841 2.8712859915699045E-4 30-31 0.0 0.0 0.0 0.016682171611021146 2.8712859915699045E-4 32-33 0.0 0.0 0.0 0.039652459543580376 2.8712859915699045E-4 34-35 0.0 0.0 0.0 0.07071977397236674 3.445543189883885E-4 36-37 0.0 0.0 0.0 0.12254648612020351 3.445543189883885E-4 38-39 0.0 0.0 0.0 0.2013345737288817 3.445543189883885E-4 40-41 0.0 0.0 0.0 0.281730581492839 3.445543189883885E-4 42-43 0.0 0.0 0.0 0.36367708369224405 3.445543189883885E-4 44-45 0.0 0.0 0.0 0.4604107087482342 3.445543189883885E-4 46-47 0.0 0.0 0.0 0.5726492781587018 3.445543189883885E-4 48-49 0.0 0.0 0.0 0.6885918064982944 3.445543189883885E-4 50-51 0.0 0.0 0.0 0.8129184899332713 3.445543189883885E-4 52-53 0.0 0.0 0.0 0.9279422067555616 3.445543189883885E-4 54-55 0.0 0.0 0.0 1.0526421573694424 3.445543189883885E-4 56-57 0.0 0.0 0.0 1.1982450700019525 3.445543189883885E-4 58-59 0.0 0.0 0.0 1.3535242164260528 3.445543189883885E-4 60-61 0.0 0.0 0.0 1.52057563541559 3.445543189883885E-4 62-63 0.0 0.0 0.0 1.6785537906717662 3.445543189883885E-4 64-65 0.0 0.0 0.0 1.8371062031262562 4.019800388197866E-4 66-67 0.0 0.0 0.0 2.022706129621335 4.019800388197866E-4 68-69 0.0 0.0 0.0 2.2213416945181406 4.019800388197866E-4 70-71 0.0 0.0 0.0 2.4345921051120376 4.019800388197866E-4 72-73 0.0 0.0 0.0 2.657375185197947 4.019800388197866E-4 74-75 0.0 0.0 0.0 2.8698503485741194 4.019800388197866E-4 76-77 0.0 0.0 0.0 3.093064121558764 4.019800388197866E-4 78-79 0.0 0.0 0.0 3.341660062708886 4.019800388197866E-4 80-81 0.0 0.0 0.0 3.609551045722358 4.019800388197866E-4 82-83 0.0 0.0 0.0 3.8770400486970105 4.594057586511847E-4 84-85 0.0 0.0 0.0 4.149381524997416 4.594057586511847E-4 86-87 0.0 0.0 0.0 4.431399235089412 5.168314784825828E-4 88-89 0.0 0.0 0.0 4.731879314107202 5.168314784825828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9305 0.0 30.920504 1 GTATCAA 13370 0.0 29.299309 1 TAAGGTG 805 0.0 26.568895 5 GTAAGGT 775 0.0 25.144272 4 TATCAAC 15770 0.0 24.772627 2 ATCAACG 15880 0.0 24.51265 3 CAACGCA 16040 0.0 24.268137 5 TCAACGC 16100 0.0 24.207218 4 AACGCAG 16365 0.0 23.784132 6 GAGTACT 9285 0.0 23.483173 12-13 GGTAAGG 820 0.0 23.184782 3 TACCGTA 105 0.0021940712 22.62366 7 GTACATG 8330 0.0 22.453636 1 AGGTAAG 815 0.0 22.159397 2 GTACTTT 9820 0.0 22.084444 14-15 TACATGG 8280 0.0 21.868881 2 ACGCAGA 17955 0.0 21.538782 7 CGCAGAG 18090 0.0 21.35178 8 AGTACTT 9560 0.0 20.745531 12-13 TATAACG 115 0.003712216 20.66351 2 >>END_MODULE