FastQCFastQC Report
Thu 26 May 2016
SRR938034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741380
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113960.6544235031986126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.5715581894819052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64110.36815628983909315No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36110.2073642743111785No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.1437940024578208No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20670.11869896289149985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.10778807612353422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89300.031.756691
TAAGGTG8800.028.0661725
GTATCAA126000.027.219431
GTAAGGT8750.026.5980954
GGTAAGG9300.023.494293
AGGTAAG8700.023.4780252
GTACATG83100.022.693521
TATCAAC150900.022.6649952
TACATGG82500.022.5130942
TCAACGC153200.022.3531654
GAGTACT91450.022.3330112-13
ATCAACG151900.022.3268723
CAACGCA154400.022.117915
TATAACG1302.9414907E-421.9240252
GTACTTT93650.021.5801414-15
AACGCAG158600.021.5621386
ACATGGG82700.021.0214653
TACCTGG13400.020.560592
AGTACTT93700.019.84516312-13
AGAGTAC137550.019.76866710-11
Produced by FastQC (version 0.11.5)