FastQCFastQC Report
Thu 26 May 2016
SRR938033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749847
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121050.6917747665938794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100260.5729643791714362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64000.36574626238751157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35940.20538938547198698No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.13738343980930903No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19470.1112668707607008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.10532349399690374No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17670.10098025713105203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93100.030.74631
GTATCAA133800.029.5233421
TATCAAC158700.024.8534472
ATCAACG158200.024.841883
TCAACGC159050.024.7394
CAACGCA160100.024.5173875
AACGCAG163700.023.9491846
GAGTACT89850.023.52037412-13
GTACATG84950.023.2502841
GTACTTT94650.022.28651614-15
TACATGG85750.022.2226432
ACATGGG85950.021.728623
ACGCAGA179850.021.6524757
CGCAGAG180900.021.447338
GTATTAG4550.020.9705521
AGTACTT91900.020.90048812-13
AGAGTAC139500.020.38208610-11
GCAGAGT188200.020.1348279
TAACGCA1456.129533E-419.6638574
ACTTTTT108050.019.56518716-17
Produced by FastQC (version 0.11.5)