##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749847 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.934833159699107 33.0 31.0 34.0 30.0 34.0 2 32.10841576434969 34.0 31.0 34.0 30.0 34.0 3 32.1682587106187 34.0 31.0 34.0 30.0 34.0 4 35.68955057213574 37.0 35.0 37.0 35.0 37.0 5 35.7169929713855 37.0 35.0 37.0 35.0 37.0 6 35.75871033296054 37.0 36.0 37.0 35.0 37.0 7 35.70357236947002 37.0 36.0 37.0 35.0 37.0 8 35.689805451562336 37.0 35.0 37.0 35.0 37.0 9 37.35458757251348 39.0 37.0 39.0 35.0 39.0 10-11 37.27967045118802 39.0 37.0 39.0 34.0 39.0 12-13 37.18027947586275 39.0 37.0 39.0 34.0 39.0 14-15 38.416216389204315 40.0 38.0 41.0 34.0 41.0 16-17 38.3502911968875 40.0 38.0 41.0 34.0 41.0 18-19 38.320552596884184 40.0 38.0 41.0 34.0 41.0 20-21 38.227590469338175 40.0 38.0 41.0 34.0 41.0 22-23 38.078862609130965 40.0 38.0 41.0 33.0 41.0 24-25 38.01865077346763 40.0 38.0 41.0 33.0 41.0 26-27 38.027856435448356 40.0 38.0 41.0 33.0 41.0 28-29 37.93725194259841 40.0 38.0 41.0 33.0 41.0 30-31 37.725180544356164 40.0 37.5 41.0 33.0 41.0 32-33 37.47068972315866 40.0 37.0 41.0 32.5 41.0 34-35 37.25094622558429 40.0 36.5 41.0 31.5 41.0 36-37 37.02165589334382 40.0 36.0 41.0 31.0 41.0 38-39 36.7741122509568 40.0 36.0 41.0 30.5 41.0 40-41 36.54247857098363 39.0 35.0 41.0 30.0 41.0 42-43 36.23767706548058 39.0 35.0 41.0 30.0 41.0 44-45 35.956425618925536 39.0 35.0 41.0 29.0 41.0 46-47 35.72837196623476 39.0 35.0 41.0 27.5 41.0 48-49 35.412072598347166 39.0 35.0 41.0 26.0 41.0 50-51 34.733747007595525 38.5 34.5 40.5 24.5 40.5 52-53 34.80266131838955 38.5 34.5 40.0 24.5 41.0 54-55 34.92958870118359 39.0 35.0 40.0 25.0 41.0 56-57 34.71374011556439 38.5 35.0 40.0 24.0 41.0 58-59 34.4497293191919 38.0 34.0 40.0 23.0 41.0 60-61 34.06937720840736 38.0 34.0 40.0 22.0 41.0 62-63 33.65355456791365 37.0 34.0 40.0 21.5 41.0 64-65 33.22942120082499 36.5 33.0 39.5 19.5 41.0 66-67 32.795451545192236 36.0 33.0 39.0 18.0 41.0 68-69 32.31041113880242 35.5 32.0 39.0 14.0 40.5 70-71 31.816588250287026 35.0 31.5 38.5 9.0 40.0 72-73 31.34285111784059 35.0 31.0 37.5 7.0 40.0 74-75 30.83077349048231 35.0 31.0 37.0 3.5 39.0 76-77 30.30205697983881 35.0 30.0 36.0 2.0 39.0 78-79 29.737017864990484 34.0 29.5 36.0 2.0 38.5 80-81 29.206424333098838 34.0 29.0 35.0 2.0 37.0 82-83 28.665741919150648 34.0 29.0 35.0 2.0 37.0 84-85 28.187361237868224 34.0 27.5 35.0 2.0 36.0 86-87 27.696187152362462 34.0 26.5 35.0 2.0 36.0 88-89 27.131690084904566 33.0 25.5 35.0 2.0 35.5 90-91 26.52333461154032 33.0 25.0 35.0 2.0 35.0 92-93 25.919532679142804 32.5 23.5 35.0 2.0 35.0 94-95 25.313292247836525 32.0 19.5 35.0 2.0 35.0 96-97 24.470972319294198 32.0 11.0 34.0 2.0 35.0 98-99 23.41826713992709 31.0 2.0 34.0 2.0 35.0 100-101 20.541783653085098 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44869530796326984 1101 2 0.32444466415736173 1101 3 0.2501472409658092 1101 4 0.23557488455975317 1101 5 0.30319386988394115 1101 6 0.3249699566192703 1101 7 0.2689831131508882 1101 8 0.24840740650486026 1101 9 0.3358797408049128 1101 10-11 0.3292714624544075 1101 12-13 0.25114324358481355 1101 14-15 0.3217033122364441 1101 16-17 0.43615195747876356 1101 18-19 0.42934517615874057 1101 20-21 0.4661506806956197 1101 22-23 0.5809640888763283 1101 24-25 0.4661635231437131 1101 26-27 0.502687990408532 1101 28-29 0.39483601369407495 1101 30-31 0.4955678624210904 1101 32-33 0.5532520841490438 1101 34-35 0.5024915202051403 1101 36-37 0.4595108248193114 1101 38-39 0.5557785191199471 1101 40-41 0.5169932475688412 1101 42-43 0.6835502167454948 1101 44-45 0.7034520215237521 1101 46-47 0.8100193668815834 1101 48-49 0.860529379502232 1101 50-51 0.7394285196020931 1101 52-53 0.9059166659748925 1101 54-55 0.7646060764872473 1101 56-57 0.7878778557334343 1101 58-59 0.6643414935118273 1101 60-61 0.8680591066574763 1101 62-63 0.9541062578905439 1101 64-65 1.0840879083683888 1101 66-67 0.8955104082679881 1101 68-69 0.7737308971053096 1101 70-71 1.0695741077996352 1101 72-73 1.044372273301633 1101 74-75 1.0398970424070235 1101 76-77 1.0814901762793703 1101 78-79 1.0468365952022864 1101 80-81 1.135400387617139 1101 82-83 1.1832183164575412 1101 84-85 0.9220829529469974 1101 86-87 1.1591064583115092 1101 88-89 1.1099425151361828 1101 90-91 1.0054335988622292 1101 92-93 0.9528850698213986 1101 94-95 1.121309813386393 1101 96-97 1.0524771638304813 1101 98-99 1.1963893482061074 1101 100-101 1.4383749821393081 1102 1 0.19488767017949726 1102 2 0.1814409641245902 1102 3 0.19685966115053333 1102 4 0.11713592720292354 1102 5 -1.9697125158302242E-4 1102 6 0.12465484176819785 1102 7 0.03197883659219514 1102 8 0.055649519748769194 1102 9 0.06680482797484188 1102 10-11 0.22815059340081945 1102 12-13 0.16280229563405157 1102 14-15 0.1560905165201092 1102 16-17 0.22492569474879076 1102 18-19 0.24264949891848175 1102 20-21 0.21023951307267907 1102 22-23 0.22984164029966792 1102 24-25 0.23664144733449888 1102 26-27 0.196040370157462 1102 28-29 0.21446375959619957 1102 30-31 0.2644214445819628 1102 32-33 0.3647202113480361 1102 34-35 0.36290472343842595 1102 36-37 0.3514080689464478 1102 38-39 0.27938863754468457 1102 40-41 0.4647558531500877 1102 42-43 0.4531827012168108 1102 44-45 0.2865795561646607 1102 46-47 0.3113236580508598 1102 48-49 0.2742426012949082 1102 50-51 0.4266191798822234 1102 52-53 0.28994749036747436 1102 54-55 0.31026021621975275 1102 56-57 0.24868890133821253 1102 58-59 0.34603008264233637 1102 60-61 0.35066164732032234 1102 62-63 0.3898139434548895 1102 64-65 0.1377217986152317 1102 66-67 0.24746427829692408 1102 68-69 0.2827085113147092 1102 70-71 0.34776681751094074 1102 72-73 0.33807254300519674 1102 74-75 0.31104488181691536 1102 76-77 0.16073425602948888 1102 78-79 0.3856531770354046 1102 80-81 0.34549452719169693 1102 82-83 0.418089842000402 1102 84-85 0.41508211185096755 1102 86-87 0.4272012770809823 1102 88-89 0.3179171322005594 1102 90-91 0.3907541038357536 1102 92-93 0.6572727874500615 1102 94-95 0.6332421229255267 1102 96-97 0.7403562987257004 1102 98-99 1.1098508335106203 1102 100-101 0.6587000874737541 1103 1 0.18993207347556762 1103 2 0.21269477823950922 1103 3 0.20552980921377895 1103 4 0.15385295356123407 1103 5 0.04136479753476863 1103 6 0.12106010260607292 1103 7 0.11757252095909365 1103 8 0.14750740382057614 1103 9 0.2031126639538101 1103 10-11 0.1686393898406422 1103 12-13 0.05174739962618702 1103 14-15 0.20185896246633206 1103 16-17 0.1489696139438621 1103 18-19 0.17580287756645419 1103 20-21 0.30170212886780945 1103 22-23 0.2720327030562544 1103 24-25 0.2846682225612227 1103 26-27 0.19534781086815656 1103 28-29 0.2993404370136119 1103 30-31 0.2746885287375278 1103 32-33 0.33139394481927553 1103 34-35 0.2792655455217954 1103 36-37 0.18751655385216282 1103 38-39 0.2183474956784579 1103 40-41 0.3225242184750172 1103 42-43 0.32265757667371275 1103 44-45 0.389333445115561 1103 46-47 0.325860871308727 1103 48-49 0.29613930675807865 1103 50-51 0.3073465108359841 1103 52-53 0.38674492153423756 1103 54-55 0.3546926255310652 1103 56-57 0.2841917561157459 1103 58-59 0.3024384115682537 1103 60-61 0.27657385297329284 1103 62-63 0.23699105984777447 1103 64-65 0.10837340900686598 1103 66-67 0.12658926108092317 1103 68-69 0.26466184577026297 1103 70-71 0.3067100515370065 1103 72-73 0.31524029463787073 1103 74-75 0.20954678145610117 1103 76-77 0.25011490161941197 1103 78-79 0.3911796545978632 1103 80-81 0.3156469200950802 1103 82-83 0.31582471962959957 1103 84-85 0.5046389298928169 1103 86-87 0.33905215904431785 1103 88-89 0.35672769941240645 1103 90-91 0.8374764937494348 1103 92-93 0.9493377861269039 1103 94-95 0.9051183931307705 1103 96-97 0.6407955354660153 1103 98-99 0.5891916275482281 1103 100-101 0.4353434647892378 1104 1 0.3958722232558429 1104 2 0.22810113180424452 1104 3 0.27900907797992147 1104 4 0.25179460001900367 1104 5 0.3174915959390603 1104 6 0.2802463123547341 1104 7 0.23063708064895394 1104 8 0.2915580404711662 1104 9 0.16057094266216865 1104 10-11 0.4321522142848764 1104 12-13 0.22385106845534608 1104 14-15 0.25729546057860375 1104 16-17 0.3851775691223125 1104 18-19 0.4311328569730719 1104 20-21 0.4009280483960751 1104 22-23 0.4007900457785212 1104 24-25 0.518310972396641 1104 26-27 0.5079394190649964 1104 28-29 0.47904266289938136 1104 30-31 0.5410448997447403 1104 32-33 0.4898219692612571 1104 34-35 0.5134226024124189 1104 36-37 0.5700709772093333 1104 38-39 0.49226655412202547 1104 40-41 0.564268755039528 1104 42-43 0.6743978862496292 1104 44-45 0.48987792485133497 1104 46-47 0.5581412789001448 1104 48-49 0.651897541297572 1104 50-51 0.7003782041071247 1104 52-53 0.5441369469440005 1104 54-55 0.6048160592243121 1104 56-57 0.5713750133023296 1104 58-59 0.6279777218587554 1104 60-61 0.570307008318288 1104 62-63 0.557210237294079 1104 64-65 0.5649022425549504 1104 66-67 0.6783922018271298 1104 68-69 0.7343479725264714 1104 70-71 0.7002987789110087 1104 72-73 0.7241887802365738 1104 74-75 0.7218796008926525 1104 76-77 0.7386848009594118 1104 78-79 0.7772427949760861 1104 80-81 0.893737193379387 1104 82-83 0.952207169960726 1104 84-85 0.8394716367972279 1104 86-87 0.7538215455190809 1104 88-89 0.9028442503585161 1104 90-91 0.9637934714736964 1104 92-93 0.9819098629638496 1104 94-95 1.283992487950357 1104 96-97 1.216673321339858 1104 98-99 1.2772576290561553 1104 100-101 0.9642876108672098 1105 1 0.3360722622192043 1105 2 0.27707929067734227 1105 3 0.2818785669646502 1105 4 0.2441446587346192 1105 5 0.13585165795574028 1105 6 0.279804930562527 1105 7 0.28554362270502764 1105 8 0.24875482978222152 1105 9 0.22493590756808857 1105 10-11 0.306602093606692 1105 12-13 0.2827877137643213 1105 14-15 0.3259592473841053 1105 16-17 0.28383580229635896 1105 18-19 0.3364094494650445 1105 20-21 0.3941182545230646 1105 22-23 0.29972494585373965 1105 24-25 0.4683059666874314 1105 26-27 0.29495782248727664 1105 28-29 0.3730931069193204 1105 30-31 0.26901893364286167 1105 32-33 0.3809880424582488 1105 34-35 0.38106708476797024 1105 36-37 0.4572815254562528 1105 38-39 0.37323653880726226 1105 40-41 0.47237830836791517 1105 42-43 0.4716220175561503 1105 44-45 0.5242974181653253 1105 46-47 0.40967194360421644 1105 48-49 0.4772346487552639 1105 50-51 0.38184311137973737 1105 52-53 0.28023629539006123 1105 54-55 0.19627949860653615 1105 56-57 0.3740098765788247 1105 58-59 0.31981341013024434 1105 60-61 0.3160903747524344 1105 62-63 0.2858086319496067 1105 64-65 0.22224656499838602 1105 66-67 0.35877039668721267 1105 68-69 0.3767998027219903 1105 70-71 0.7181554627504205 1105 72-73 0.7416218390225353 1105 74-75 0.6141587679426372 1105 76-77 0.6602105005410941 1105 78-79 0.7678846554223249 1105 80-81 0.7324762416993629 1105 82-83 0.861678073261249 1105 84-85 0.7594888591427136 1105 86-87 0.7463676048083236 1105 88-89 0.6153854798148153 1105 90-91 0.6499906341835988 1105 92-93 0.8272046992365745 1105 94-95 1.2537649378797369 1105 96-97 1.3161714313464046 1105 98-99 1.050645283353763 1105 100-101 1.0758677441543654 1106 1 0.11758914476723703 1106 2 0.07686194373687272 1106 3 -0.004943737808851267 1106 4 0.002932028720501023 1106 5 0.06561607710018791 1106 6 0.1274228246863629 1106 7 0.15946912413769354 1106 8 0.029193273312664303 1106 9 0.06816306166706454 1106 10-11 -0.37748396250864147 1106 12-13 -0.12868538415960273 1106 14-15 0.05877465346616617 1106 16-17 0.08355126030941307 1106 18-19 0.15491961084929784 1106 20-21 -0.10679294348994972 1106 22-23 -0.1700333013012596 1106 24-25 -0.039218551208854535 1106 26-27 -0.014869909323181218 1106 28-29 -0.015366931937663253 1106 30-31 0.03850309137369834 1106 32-33 0.08557920622668291 1106 34-35 -0.03618860316828432 1106 36-37 -0.1679990760804344 1106 38-39 -0.06964899899368504 1106 40-41 -0.15196537853302772 1106 42-43 -0.25209302235439424 1106 44-45 -0.10698544311589586 1106 46-47 0.06284363275495508 1106 48-49 -0.009485950923192377 1106 50-51 0.040697842325798206 1106 52-53 0.18845549392737126 1106 54-55 -0.1666990172290994 1106 56-57 -0.19834939957290487 1106 58-59 0.06709549493536571 1106 60-61 -0.08818621665025006 1106 62-63 0.15854934368617535 1106 64-65 0.14370683779367965 1106 66-67 -0.018439366338263596 1106 68-69 -0.34397209415269714 1106 70-71 -0.02231171703200019 1106 72-73 0.2218593610683186 1106 74-75 0.11914347899564248 1106 76-77 0.17808006914889773 1106 78-79 0.2574976285459627 1106 80-81 0.10726449434610075 1106 82-83 0.2650116112214711 1106 84-85 0.22429308927828018 1106 86-87 0.3299129481916019 1106 88-89 -4.0552202184684916E-4 1106 90-91 0.12564877233329597 1106 92-93 0.23016957696647466 1106 94-95 0.2682638038596288 1106 96-97 0.4197868566744596 1106 98-99 0.5242542214563422 1106 100-101 0.42024036640372486 1107 1 0.1121075357286685 1107 2 0.12327745886101127 1107 3 0.09509719546990425 1107 4 0.07119556375609193 1107 5 0.09291214389828184 1107 6 -0.03439191537163566 1107 7 -0.08774592299830886 1107 8 0.01000607335281245 1107 9 -0.01190839121339593 1107 10-11 -0.006814460310629045 1107 12-13 0.030824702521712766 1107 14-15 0.09918458221716264 1107 16-17 -0.05860938947893857 1107 18-19 0.1449609048427689 1107 20-21 0.20452377500984653 1107 22-23 0.25475705287355055 1107 24-25 0.29960109613762853 1107 26-27 0.25272285317126375 1107 28-29 0.24497546474501064 1107 30-31 0.3076671585419106 1107 32-33 0.15338839755133904 1107 34-35 0.20849767696903854 1107 36-37 0.22513213154538647 1107 38-39 0.22516360971082605 1107 40-41 0.16890651598771456 1107 42-43 0.2130842061960223 1107 44-45 0.20793607006665837 1107 46-47 0.24417476416018502 1107 48-49 0.0730278542563596 1107 50-51 0.25455948157030406 1107 52-53 0.23207750881704925 1107 54-55 0.33607299695483306 1107 56-57 0.2551844284100895 1107 58-59 0.2130726632621034 1107 60-61 0.32651228073770966 1107 62-63 0.24278893353785946 1107 64-65 0.2605551948949767 1107 66-67 0.21730887360916995 1107 68-69 0.20311811432473093 1107 70-71 0.3231386509754195 1107 72-73 0.29633957900274055 1107 74-75 0.33439935903761153 1107 76-77 0.3879134659993859 1107 78-79 0.42662891772468114 1107 80-81 0.35087161014963897 1107 82-83 0.45249794719115854 1107 84-85 0.36182231893279493 1107 86-87 0.4530410348509619 1107 88-89 0.4944972653619182 1107 90-91 0.403209180285959 1107 92-93 0.6354322478550714 1107 94-95 0.59038392813369 1107 96-97 0.5770390260598148 1107 98-99 0.24409415822148262 1107 100-101 0.33704339891666635 1108 1 0.28870513881081905 1108 2 0.1833637521571987 1108 3 0.12454750770488943 1108 4 0.15217904857678377 1108 5 0.13319239181944909 1108 6 0.1565737253399888 1108 7 0.0918037414314199 1108 8 0.024284019056032946 1108 9 0.285928723174699 1108 10-11 0.2520358899106583 1108 12-13 0.029876817304142378 1108 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2316 70-71 -0.9501332495193573 2316 72-73 -0.8938179659905572 2316 74-75 -0.8770117999827569 2316 76-77 -0.8920052813865702 2316 78-79 -0.9845957100130036 2316 80-81 -0.8464969257715858 2316 82-83 -0.8729650146695285 2316 84-85 -0.8272273080178643 2316 86-87 -0.8986561458574016 2316 88-89 -1.0216617245808415 2316 90-91 -0.9048930920835794 2316 92-93 -0.9677201334676724 2316 94-95 -1.0706865355067308 2316 96-97 -1.1075262840950693 2316 98-99 -1.0143598675447585 2316 100-101 -1.0523708780411525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4683.0 3 1273.0 4 1280.0 5 1477.0 6 1654.0 7 2005.0 8 2697.0 9 3199.0 10 4094.0 11 5272.0 12 6653.0 13 8434.0 14 10007.0 15 12521.0 16 15529.0 17 21906.0 18 19356.0 19 13917.0 20 13296.0 21 13342.0 22 14202.0 23 15473.0 24 17246.0 25 19079.0 26 21779.0 27 25108.0 28 29699.0 29 34923.0 30 41897.0 31 50951.0 32 63406.0 33 82590.0 34 113630.0 35 170304.0 36 252178.0 37 344376.0 38 259269.0 39 31138.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70078608117172 18.414445332671757 14.56248679144729 22.322281794709227 2 15.655626867165054 19.95740362212153 40.32436141168398 24.062608099029433 3 19.582588807090275 24.522793224215565 29.83998284611536 26.0546351225788 4 12.724844956696527 16.623672946551878 36.86173482662885 33.78974727012275 5 13.538514507818544 37.53868379858745 33.71745942597847 15.205342267615535 6 31.710759285479327 36.13300725104046 17.070007579983123 15.08622588349709 7 28.388388468530707 33.59105728391362 19.97900083550597 18.041553412049698 8 26.883622052556678 34.12999140617897 19.19069420120062 19.79569234006373 9 26.796164355678194 16.820252915584835 20.0907623546067 36.29282037413027 10-11 25.39370219463256 26.518226553655676 26.41757238735955 21.67049886435221 12-13 26.701535785899605 24.09101440818323 27.152174099595815 22.05527570632135 14-15 23.1399762935212 25.274388483202763 26.249922093397583 25.335713129878457 16-17 21.822385229609402 28.59455877071997 26.97753197056718 22.605524029103453 18-19 21.854637121733468 26.623776708419665 29.959689881699997 21.56189628814687 20-21 22.673541977455365 25.49083525418262 30.26083231750645 21.57479045085557 22-23 22.295747356912145 25.48026186562532 30.314059766365816 21.909931011096724 24-25 22.15349663509001 25.770074207618894 30.35600095633682 21.720428200954277 26-27 21.669778949303677 25.941969438728346 30.43243430252988 21.955817309438093 28-29 21.58705169759617 26.665115044936034 30.165413551033105 21.582419706434695 30-31 21.728626634660884 25.982097541378522 30.042361431796376 22.246914392164207 32-33 21.51152300898949 26.163559561685606 30.081088105791114 22.24382932353379 34-35 22.348585143853192 26.190636861118723 29.79753211527013 21.66324587975796 36-37 22.388368246968028 25.64345089140708 30.013735949530396 21.954444912094498 38-39 21.800315869964482 26.246821647812133 29.89570574068648 22.0571567415369 40-41 22.16447766185986 26.134559118852312 29.883610407170618 21.81735281211721 42-43 21.94753685041013 26.07921355300413 29.488818028365486 22.484431568220245 44-45 22.011222776165233 26.228737943546587 29.568734216720642 22.191305063567533 46-47 22.575092870182388 26.627139615531593 28.895610113582155 21.90215740070386 48-49 22.33089738371694 26.685795654619536 28.705286300298628 22.278020661364895 50-51 22.12426435134731 27.024854070686864 28.76357293378869 22.087308644177135 52-53 22.576173564201984 26.928311705831682 28.401422664890053 22.09409206507629 54-55 22.06701451687767 26.902067666164402 28.56842756308977 22.462490253868157 56-57 22.305263558032728 27.031779245013926 28.377729119727864 22.28522807722549 58-59 22.469438400510786 26.630726021794743 28.3857833723978 22.514052205296668 60-61 22.027186315264764 26.828665276424886 28.950613261260273 22.19353514705008 62-63 21.989854244069733 26.84955701629037 28.994512717919406 22.16607602172049 64-65 21.739477125117002 27.10495366433976 28.667204927227647 22.48836428331559 66-67 21.857344743772387 27.029051465576494 28.430312062838386 22.683291727812733 68-69 22.12276708206335 26.742286868063324 28.630819947614576 22.504126102258745 70-71 22.223127429230203 26.819330304987112 28.266213186541155 22.691329079241534 72-73 22.113553839157262 26.843958585769368 28.428020607069897 22.614466968003473 74-75 21.96628956560015 27.539344084238927 28.03293870154518 22.46142764861574 76-77 22.18820191883657 27.02710658891632 28.09272995357672 22.691961538670398 78-79 22.337369505514133 27.248477504793968 28.013740322760412 22.40041266693149 80-81 22.525312165271835 27.375491125166757 27.766790850696804 22.3324058588646 82-83 22.461643460161014 27.32753597010758 27.909996921931086 22.300823647800325 84-85 22.39679639125318 27.488486888170343 27.734467658972967 22.380249061603507 86-87 22.52838870572878 27.420016248652974 27.610536419872506 22.44105862574574 88-89 22.436521553200237 27.44599193773844 27.405429077310693 22.71205743175063 90-91 22.458804698004037 27.403041415290442 27.49818391605733 22.639969970648195 92-93 22.73556969478697 27.13311838652695 27.628296375059307 22.50301554362677 94-95 22.538240480476084 26.979436593303802 27.75765438564793 22.72466854057219 96-97 22.679267752686584 26.695828189046022 27.815427116156606 22.809476942110795 98-99 22.602952924984958 26.989780095553463 27.238396313917278 23.168870665544304 100-101 22.995272454268 26.266830271748727 27.57335252172504 23.16454475225823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1504.0 1 1027.5 2 799.0 3 1013.0 4 783.0 5 696.0 6 1319.5 7 2930.5 8 4747.5 9 5411.0 10 4335.5 11 3005.0 12 2759.5 13 2835.0 14 2992.5 15 3187.5 16 3269.5 17 3338.5 18 3548.0 19 3858.5 20 4183.0 21 4718.0 22 5427.0 23 6257.5 24 7472.5 25 9164.0 26 11385.0 27 13793.5 28 15677.5 29 17787.5 30 20140.5 31 23368.0 32 27950.0 33 30275.5 34 31234.5 35 33164.0 36 36499.5 37 40133.0 38 42482.0 39 44233.5 40 46118.0 41 48814.0 42 50888.5 43 52632.5 44 54533.5 45 57608.5 46 59816.5 47 62901.0 48 69076.5 49 68871.5 50 64151.5 51 61638.5 52 59923.0 53 59984.0 54 60700.5 55 57478.5 56 52181.5 57 47621.0 58 44130.0 59 39494.5 60 32519.0 61 27220.0 62 23020.0 63 17437.5 64 12584.0 65 9790.5 66 7594.0 67 6165.0 68 4751.5 69 3368.5 70 2546.0 71 1738.5 72 1246.5 73 1010.5 74 744.0 75 565.0 76 412.0 77 240.0 78 135.5 79 85.5 80 50.5 81 26.5 82 16.5 83 11.5 84 7.0 85 6.5 86 6.0 87 4.5 88 4.0 89 5.0 90 3.5 91 3.0 92 3.5 93 3.0 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4886712952618143 2 0.04983292825029846 3 0.05554771360010332 4 0.0652628486947716 5 0.07863544641331499 6 0.1041233890734447 7 0.06892031131864672 8 0.0523474338042126 9 0.050518702492275046 10-11 0.07346356567174159 12-13 0.10755226028332764 14-15 0.05474764365113065 16-17 0.06006239402644917 18-19 0.05889086302973917 20-21 0.07266349572276891 22-23 0.06214829067912794 24-25 0.08623611092855546 26-27 0.06460564837954405 28-29 0.06509140513427746 30-31 0.06883458953839965 32-33 0.06820596314992111 34-35 0.0699489726816116 36-37 0.06577717937625405 38-39 0.04054640205686554 40-41 0.04368953399925822 42-43 0.04083214132435579 44-45 0.021087557940779964 46-47 0.028373909261781172 48-49 0.0282881874815341 50-51 0.026030847268361176 52-53 0.025945125488114104 54-55 0.02485931627165118 56-57 0.025745108000870934 58-59 0.022201941083991913 60-61 0.02920255313750288 62-63 0.019830305163822896 64-65 0.02197334966999972 66-67 0.015972825052704608 68-69 0.017972999925136315 70-71 0.04043210634986944 72-73 0.03666034801899823 74-75 0.030688397328452143 76-77 0.02294486317946655 78-79 0.014401259081508268 80-81 0.016744321074928264 82-83 0.021344723281521184 84-85 0.01828731311937558 86-87 0.00934367404693096 88-89 0.040574975983614564 90-91 0.05094731139351041 92-93 0.032974311468374096 94-95 0.05229028595071455 96-97 0.04220368980830896 98-99 0.03225996329964848 100-101 0.169014776720479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1749847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.93864826273031 #Duplication Level Percentage of deduplicated Percentage of total 1 79.51365488004224 46.06913682178897 2 11.985193669253256 13.88811840727133 3 3.592167688729024 6.2437602065404745 4 1.5947755568833135 3.6959656019304865 5 0.8396626230222377 2.432445868732348 6 0.5336120838396661 1.8550057700597384 7 0.34189565251868265 1.3866280367685633 8 0.24420985082560975 1.1319350919423075 9 0.1804842506638897 0.94113121645598 >10 1.0670501651544304 11.23818151124838 >50 0.06906468534293485 2.708673955561122 >100 0.03228717963835371 3.524901365799218 >500 0.0039611427242762015 1.6735951114218712 >1k 0.0016834856578173854 1.5735316922330151 >5k 9.902856810690504E-5 0.36720520803249945 >10k+ 1.9805713621381008E-4 1.269784134213632 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12105 0.6917747665938794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10026 0.5729643791714362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6400 0.36574626238751157 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3594 0.20538938547198698 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2404 0.13738343980930903 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1947 0.1112668707607008 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1843 0.10532349399690374 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1767 0.10098025713105203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.7147853498048685E-5 2 0.0 0.0 0.0 0.0 5.7147853498048685E-5 3 0.0 0.0 0.0 0.0 5.7147853498048685E-5 4 0.0 0.0 0.0 0.0 5.7147853498048685E-5 5 0.0 0.0 0.0 0.0 5.7147853498048685E-5 6 0.0 0.0 0.0 0.0 5.7147853498048685E-5 7 0.0 0.0 0.0 0.0 5.7147853498048685E-5 8 0.0 0.0 0.0 0.0 5.7147853498048685E-5 9 0.0 0.0 0.0 0.0 5.7147853498048685E-5 10-11 0.0 0.0 0.0 0.0 5.7147853498048685E-5 12-13 0.0 0.0 0.0 0.0 5.7147853498048685E-5 14-15 0.0 0.0 0.0 1.1429570699609737E-4 5.7147853498048685E-5 16-17 0.0 0.0 0.0 1.7144356049414607E-4 5.7147853498048685E-5 18-19 0.0 0.0 0.0 1.7144356049414607E-4 5.7147853498048685E-5 20-21 0.0 0.0 0.0 3.143131942392678E-4 5.7147853498048685E-5 22-23 0.0 0.0 0.0 4.8575675473341384E-4 5.7147853498048685E-5 24-25 0.0 0.0 0.0 0.001171530996709998 5.7147853498048685E-5 26-27 0.0 0.0 0.0 0.0023716359201690204 8.572178024707303E-5 28-29 0.0 0.0 0.0 0.005743359276553893 1.1429570699609737E-4 30-31 0.0 0.0 0.0 0.01505845939673583 1.428696337451217E-4 32-33 0.0 0.0 0.0 0.0367460697992453 1.7144356049414607E-4 34-35 0.0 0.0 0.0 0.06966323341412134 1.7144356049414607E-4 36-37 0.0 0.0 0.0 0.11958188344466687 1.7144356049414607E-4 38-39 0.0 0.0 0.0 0.19338833623739676 1.7144356049414607E-4 40-41 0.0 0.0 0.0 0.2738525139626493 1.7144356049414607E-4 42-43 0.0 0.0 0.0 0.3546881527356392 1.7144356049414607E-4 44-45 0.0 0.0 0.0 0.4514394687078356 1.7144356049414607E-4 46-47 0.0 0.0 0.0 0.5641350358059876 1.7144356049414607E-4 48-49 0.0 0.0 0.0 0.6822882229132032 1.7144356049414607E-4 50-51 0.0 0.0 0.0 0.8064990824912122 1.7144356049414607E-4 52-53 0.0 0.0 0.0 0.9229378339934863 1.7144356049414607E-4 54-55 0.0 0.0 0.0 1.0462629018422753 2.2859141399219474E-4 56-57 0.0 0.0 0.0 1.193761511720739 2.2859141399219474E-4 58-59 0.0 0.0 0.0 1.347432089776992 2.2859141399219474E-4 60-61 0.0 0.0 0.0 1.513189438848082 2.2859141399219474E-4 62-63 0.0 0.0 0.0 1.6744892553463244 2.2859141399219474E-4 64-65 0.0 0.0 0.0 1.831160095711225 2.2859141399219474E-4 66-67 0.0 0.0 0.0 2.0182621680638366 2.2859141399219474E-4 68-69 0.0 0.0 0.0 2.2243087538510506 2.2859141399219474E-4 70-71 0.0 0.0 0.0 2.440070474732934 2.2859141399219474E-4 72-73 0.0 0.0 0.0 2.6532319682806556 2.2859141399219474E-4 74-75 0.0 0.0 0.0 2.870308089792993 2.2859141399219474E-4 76-77 0.0 0.0 0.0 3.095013449747321 2.2859141399219474E-4 78-79 0.0 0.0 0.0 3.3521502165617907 2.2859141399219474E-4 80-81 0.0 0.0 0.0 3.629317306027327 2.2859141399219474E-4 82-83 0.0 0.0 0.0 3.906627265126608 2.2859141399219474E-4 84-85 0.0 0.0 0.0 4.182337084327944 2.2859141399219474E-4 86-87 0.0 0.0 0.0 4.464618906681555 2.2859141399219474E-4 88-89 0.0 0.0 0.0 4.759358961097742 2.2859141399219474E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9310 0.0 30.7463 1 GTATCAA 13380 0.0 29.523342 1 TATCAAC 15870 0.0 24.853447 2 ATCAACG 15820 0.0 24.84188 3 TCAACGC 15905 0.0 24.739 4 CAACGCA 16010 0.0 24.517387 5 AACGCAG 16370 0.0 23.949184 6 GAGTACT 8985 0.0 23.520374 12-13 GTACATG 8495 0.0 23.250284 1 GTACTTT 9465 0.0 22.286516 14-15 TACATGG 8575 0.0 22.222643 2 ACATGGG 8595 0.0 21.72862 3 ACGCAGA 17985 0.0 21.652475 7 CGCAGAG 18090 0.0 21.44733 8 GTATTAG 455 0.0 20.970552 1 AGTACTT 9190 0.0 20.900488 12-13 AGAGTAC 13950 0.0 20.382086 10-11 GCAGAGT 18820 0.0 20.134827 9 TAACGCA 145 6.129533E-4 19.663857 4 ACTTTTT 10805 0.0 19.565187 16-17 >>END_MODULE