FastQCFastQC Report
Thu 26 May 2016
SRR938033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749847
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118310.6761162547354139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100310.5732501184389264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65620.3750042146541955No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35740.204246428402026No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.14795579270644807No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20280.11589584689404274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.1129241585121442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98100.029.8799171
GTATCAA136550.026.8241461
TATAACG1109.469075E-525.9132462
TACATGG83100.024.8686082
GTACATG84250.024.8674871
ACATGGG85200.023.137143
ATCAACG159650.022.731373
TCAACGC159800.022.7080884
TATCAAC159850.022.7061752
GAGTACT92500.022.56737512-13
CAACGCA161200.022.4814055
AACGCAG165350.021.9458856
GTACTTT96600.021.28995514-15
CATGGGA37700.020.913694
AGAGTAC134850.020.34065810-11
AGTACTT96050.020.24978812-13
ACGCAGA180700.020.0553557
CATGGGG42950.020.0160964
CGCAGAG181700.019.9188398
TAAGGTG9550.019.893935
Produced by FastQC (version 0.11.5)