##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938033_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749847 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52128557525315 34.0 31.0 34.0 31.0 34.0 2 32.72949234990259 34.0 31.0 34.0 31.0 34.0 3 32.779700168071834 34.0 31.0 34.0 31.0 34.0 4 36.23584976286498 37.0 35.0 37.0 35.0 37.0 5 36.204332150182275 37.0 37.0 37.0 35.0 37.0 6 36.254082785523536 37.0 37.0 37.0 35.0 37.0 7 36.2208901692548 37.0 37.0 37.0 35.0 37.0 8 36.21932088919774 37.0 37.0 37.0 35.0 37.0 9 37.92600553076926 39.0 38.0 39.0 35.0 39.0 10-11 37.91479683652342 39.0 38.0 39.0 35.0 39.0 12-13 37.86500448324911 39.0 38.0 39.0 35.0 39.0 14-15 39.17018545049939 40.5 38.5 41.0 36.0 41.0 16-17 39.12852809417052 40.0 38.5 41.0 36.0 41.0 18-19 39.1757296495065 40.0 39.0 41.0 36.0 41.0 20-21 39.12155605604376 40.0 39.0 41.0 36.0 41.0 22-23 38.973540258091134 40.0 38.5 41.0 35.0 41.0 24-25 38.922987552626026 40.0 38.0 41.0 35.0 41.0 26-27 38.87910543036048 40.0 38.0 41.0 35.0 41.0 28-29 38.82081862014222 40.0 38.0 41.0 35.0 41.0 30-31 38.611742912380336 40.0 38.0 41.0 35.0 41.0 32-33 38.41145025822257 40.0 38.0 41.0 34.0 41.0 34-35 38.193082880960446 40.0 38.0 41.0 34.0 41.0 36-37 38.05055870598972 40.0 37.5 41.0 33.5 41.0 38-39 38.02175961669792 40.0 38.0 41.0 33.5 41.0 40-41 37.95753857337242 40.0 38.0 41.0 33.0 41.0 42-43 37.787263400743036 40.0 37.0 41.0 33.0 41.0 44-45 37.542801742095165 40.0 37.0 41.0 33.0 41.0 46-47 37.16070090699358 40.0 37.0 41.0 32.0 41.0 48-49 36.760289613891956 40.0 36.0 41.0 31.0 41.0 50-51 36.44485689320267 40.0 36.0 41.0 30.0 41.0 52-53 36.03374037844452 39.0 35.0 41.0 29.5 41.0 54-55 35.813175951954655 39.0 35.0 41.0 29.0 41.0 56-57 35.56207371273031 39.0 35.0 40.5 28.0 41.0 58-59 35.220359837174335 38.0 35.0 40.0 27.5 41.0 60-61 34.86058809713077 38.0 34.0 40.0 27.0 41.0 62-63 34.50449324998128 37.0 34.0 40.0 26.0 41.0 64-65 34.111812918500874 37.0 34.0 40.0 26.0 41.0 66-67 33.694910755054586 36.0 33.5 39.0 25.5 41.0 68-69 33.23456507911835 36.0 33.0 39.0 23.5 40.0 70-71 32.71659379362882 35.0 32.5 38.5 22.5 40.0 72-73 32.31160038563371 35.0 32.0 37.5 22.0 39.5 74-75 31.880679567985087 35.0 32.0 37.0 20.5 39.0 76-77 30.578792317271166 34.0 30.0 35.5 19.5 38.0 78-79 31.292498144123456 35.0 31.5 36.0 20.0 38.5 80-81 31.132029257415077 35.0 31.5 36.0 19.5 37.0 82-83 30.77072938376898 35.0 31.0 35.0 17.5 37.0 84-85 30.398861729054026 34.0 31.0 35.0 13.5 36.5 86-87 29.979694510434342 34.0 30.5 35.0 7.0 36.0 88-89 29.519642288725812 34.0 30.0 35.0 4.5 36.0 90-91 29.190635524134393 34.0 30.0 35.0 2.0 35.0 92-93 28.797782320397154 34.0 29.0 35.0 2.0 35.0 94-95 28.38760731652539 34.0 29.0 35.0 2.0 35.0 96-97 27.81984367776154 34.0 28.5 35.0 2.0 35.0 98-99 27.14490866915793 33.0 27.0 35.0 2.0 35.0 100-101 24.7107947151951 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1943061162742552 1101 2 0.15849735892863492 1101 3 0.18681883934700494 1101 4 0.06715429538645878 1101 5 0.10258539481488071 1101 6 0.1044257063254932 1101 7 0.13351879462636163 1101 8 0.09418249491865538 1101 9 0.17054065848374478 1101 10-11 0.17544946080475654 1101 12-13 0.1341288880714373 1101 14-15 0.18146794254517573 1101 16-17 0.18195834337210925 1101 18-19 0.27199851988600443 1101 20-21 0.1973882830112501 1101 22-23 0.23927845284783444 1101 24-25 0.19389806956645828 1101 26-27 0.17322363521257245 1101 28-29 0.20352868454799733 1101 30-31 0.24940134985993012 1101 32-33 0.19423702181744318 1101 34-35 0.187072373362696 1101 36-37 0.21383922513076925 1101 38-39 0.23641954046541258 1101 40-41 0.2854003730508836 1101 42-43 0.2384471269024928 1101 44-45 0.40705272429507033 1101 46-47 0.35349688383877265 1101 48-49 0.33377045621523393 1101 50-51 0.28092369200075495 1101 52-53 0.3535957891240358 1101 54-55 0.32457050817002653 1101 56-57 0.4234857066328672 1101 58-59 0.4253856272374108 1101 60-61 0.37437396659888833 1101 62-63 0.3417587175841561 1101 64-65 0.35171428667309357 1101 66-67 0.2693050976572451 1101 68-69 0.3230772206161632 1101 70-71 0.34383589398122893 1101 72-73 0.26818456367011834 1101 74-75 0.34531302263911456 1101 76-77 0.3030231997744508 1101 78-79 0.36394265606329057 1101 80-81 0.4800861215716026 1101 82-83 0.646088487779263 1101 84-85 0.7364563396979449 1101 86-87 0.5580685144134279 1101 88-89 0.5406177560346279 1101 90-91 0.42199356042729264 1101 92-93 0.831558525834371 1101 94-95 0.6998869548720208 1101 96-97 0.6464879833792487 1101 98-99 0.7077350622861651 1101 100-101 0.8232254288997929 1102 1 0.08919043595393106 1102 2 0.09288239136809295 1102 3 0.11229575659184121 1102 4 0.08790448986523103 1102 5 -0.008801386946366563 1102 6 0.01758313344037532 1102 7 0.105088564134455 1102 8 0.03847350624778301 1102 9 0.06901207503951667 1102 10-11 0.07084653315875755 1102 12-13 0.058043459566576416 1102 14-15 0.1508678422454537 1102 16-17 0.08043588057974915 1102 18-19 0.17341140326586668 1102 20-21 0.025398162927999124 1102 22-23 0.20689368892730187 1102 24-25 0.11714687209052954 1102 26-27 0.13609437889152076 1102 28-29 0.1440975497791399 1102 30-31 -0.09586797856635343 1102 32-33 -0.14077113247036976 1102 34-35 0.07521489372867762 1102 36-37 0.10411252210874267 1102 38-39 -6.968822307484857E-4 1102 40-41 0.03591865405593353 1102 42-43 0.06365631498815816 1102 44-45 0.15568308035906142 1102 46-47 0.2684093585170402 1102 48-49 0.18255145929136063 1102 50-51 0.18186765447129005 1102 52-53 0.0996142063629506 1102 54-55 0.010594690668114026 1102 56-57 -0.008505163723825149 1102 58-59 0.09916337254720986 1102 60-61 -0.09233699252700234 1102 62-63 -0.12012324465734281 1102 64-65 -0.006097836956172387 1102 66-67 -0.10909729374067467 1102 68-69 0.15292783722247094 1102 70-71 0.08002118783175405 1102 72-73 0.016603658524431353 1102 74-75 0.05882446036049416 1102 76-77 0.08552168935551663 1102 78-79 -8.429849777868981E-5 1102 80-81 -0.07263500178520133 1102 82-83 0.11632968095098661 1102 84-85 -0.005272685222983853 1102 86-87 -0.061235626701162005 1102 88-89 -0.2658342007970056 1102 90-91 -0.14212046534262868 1102 92-93 0.09575330472088339 1102 94-95 -0.03166816455966881 1102 96-97 0.17173665954148376 1102 98-99 0.2092119163500783 1102 100-101 0.010807416018781879 1103 1 0.07824833510490237 1103 2 0.13189301882195537 1103 3 0.12581997825112978 1103 4 0.0739976903177606 1103 5 0.05025370912174054 1103 6 0.0472170567896768 1103 7 -0.008299310497939416 1103 8 0.07652526287748174 1103 9 0.02900823806323416 1103 10-11 0.0032649890146814187 1103 12-13 0.07179536172517231 1103 14-15 0.10557397950395142 1103 16-17 0.2035302227939937 1103 18-19 0.09898824674074547 1103 20-21 0.11911201631594537 1103 22-23 0.149811015152018 1103 24-25 0.10797657342223488 1103 26-27 0.07562281152030437 1103 28-29 0.04552663682139979 1103 30-31 0.0797757693831258 1103 32-33 0.07060035140555954 1103 34-35 0.05533222756763223 1103 36-37 0.024537254481515447 1103 38-39 -0.05396534777242579 1103 40-41 0.059805324407790295 1103 42-43 0.041181263732049445 1103 44-45 0.10464607033402018 1103 46-47 -0.04393674719464258 1103 48-49 0.050565073838725993 1103 50-51 0.03088879628525376 1103 52-53 0.04145994125401131 1103 54-55 0.06617597951144205 1103 56-57 -0.03505588254102321 1103 58-59 -0.013609951360400885 1103 60-61 0.13875330219980953 1103 62-63 0.12572298590536235 1103 64-65 0.10866601171019852 1103 66-67 0.024747487131776325 1103 68-69 0.0974281027875108 1103 70-71 -0.0023273029264743172 1103 72-73 -0.12079797696804917 1103 74-75 -0.13482153677371755 1103 76-77 -0.12908584358443775 1103 78-79 -0.2280510780466969 1103 80-81 -0.06162403691630303 1103 82-83 -0.23961481427574682 1103 84-85 -0.2702041403290494 1103 86-87 -0.015175675066473104 1103 88-89 -0.10932606569811654 1103 90-91 -0.05303337829813515 1103 92-93 0.07083442056434919 1103 94-95 0.11434931159826434 1103 96-97 0.05252072399671448 1103 98-99 0.17659351222615172 1103 100-101 -0.01170384016918291 1104 1 0.07970703299548632 1104 2 0.05437806611358553 1104 3 0.02071073767428544 1104 4 0.04280835217555534 1104 5 -0.008473299154999836 1104 6 -0.006025064553725201 1104 7 0.07547664926056541 1104 8 0.02013716483948258 1104 9 0.009478988146220502 1104 10-11 0.09624273719025922 1104 12-13 0.10089476699280198 1104 14-15 0.16957065699246243 1104 16-17 0.11824510151247836 1104 18-19 0.04529529350347872 1104 20-21 0.11057768416607416 1104 22-23 0.13638505145488722 1104 24-25 0.10598844696987442 1104 26-27 0.1066102951328105 1104 28-29 0.16021658263716887 1104 30-31 0.10411963666272328 1104 32-33 0.029384575243241784 1104 34-35 0.125801613198405 1104 36-37 0.12520411292171474 1104 38-39 0.08336764749559933 1104 40-41 0.21569012244072638 1104 42-43 0.15080128488809663 1104 44-45 0.09159533099785477 1104 46-47 0.09992487062369548 1104 48-49 -0.011285053041270032 1104 50-51 -0.05440951235664215 1104 52-53 -0.04127034874131397 1104 54-55 0.026423297026504144 1104 56-57 0.06075503814069805 1104 58-59 0.17648725531051213 1104 60-61 0.15610241769717703 1104 62-63 0.022694710798042195 1104 64-65 0.0750225937151825 1104 66-67 0.22284027832276365 1104 68-69 0.1552056211161883 1104 70-71 0.15565393374550496 1104 72-73 0.08903736740843726 1104 74-75 0.04702108561905405 1104 76-77 0.002859499554197953 1104 78-79 0.11160732360302461 1104 80-81 0.032105214970016505 1104 82-83 0.10952529949424772 1104 84-85 0.23110334249572873 1104 86-87 0.11060958828687006 1104 88-89 0.07474627558936575 1104 90-91 0.21402842857420268 1104 92-93 0.21369362316159268 1104 94-95 0.3324766923563942 1104 96-97 0.42282883700148943 1104 98-99 0.4778701893770645 1104 100-101 0.5703171456686427 1105 1 0.07201312264368198 1105 2 0.08218004566865034 1105 3 -0.010691293831918358 1105 4 0.03275939092785762 1105 5 0.05400403986409685 1105 6 -0.008313147177695157 1105 7 -0.005392612437916 1105 8 -0.006917678792987658 1105 9 -0.10068111337496788 1105 10-11 -0.023016086386903112 1105 12-13 0.018523792530039884 1105 14-15 0.12072677288680467 1105 16-17 0.150082257674363 1105 18-19 0.13210274686920087 1105 20-21 0.08178318746984559 1105 22-23 0.054536125111873446 1105 24-25 0.06920206493414582 1105 26-27 -0.024257546026568377 1105 28-29 0.026458620853730963 1105 30-31 0.14346620220504036 1105 32-33 0.12585254005716706 1105 34-35 0.13796988349588446 1105 36-37 0.19785775437222952 1105 38-39 -0.020499973141994587 1105 40-41 0.13485028619506778 1105 42-43 0.03381481653770635 1105 44-45 0.08759702076293507 1105 46-47 0.16933359489841138 1105 48-49 0.15053362934783365 1105 50-51 0.020153570402612786 1105 52-53 0.002206096014596426 1105 54-55 0.07393356549486185 1105 56-57 0.0534220123653526 1105 58-59 -0.04852867502084024 1105 60-61 -0.02281769524245192 1105 62-63 0.008435034897409821 1105 64-65 0.05733096299041307 1105 66-67 -0.04945575932016766 1105 68-69 -0.031931464965772705 1105 70-71 0.07927306827190961 1105 72-73 -0.08456587060896936 1105 74-75 -0.1445827503776762 1105 76-77 0.052531016798187835 1105 78-79 0.1657087243898694 1105 80-81 0.11381513141525801 1105 82-83 0.24189045651116814 1105 84-85 0.2825478278276492 1105 86-87 0.3470667772970373 1105 88-89 0.2809767600878992 1105 90-91 0.1002796055923163 1105 92-93 0.19296559010767922 1105 94-95 0.1898985356479308 1105 96-97 0.34104501870061554 1105 98-99 0.38694756952414977 1105 100-101 0.4061148324898305 1106 1 -0.179566458689699 1106 2 -0.04153549198297668 1106 3 -0.009036470637845184 1106 4 -0.06010283599893995 1106 5 -0.08745530381450806 1106 6 -0.0711788417007142 1106 7 0.026620103187674715 1106 8 -0.08083256174916897 1106 9 -0.07667705180772089 1106 10-11 -0.005483322579976857 1106 12-13 -0.1294549638804554 1106 14-15 -0.22055968872159326 1106 16-17 -0.07220642220013218 1106 18-19 -0.036013452446233885 1106 20-21 -0.06260386927097983 1106 22-23 -0.05305724696784608 1106 24-25 -0.03135606873588159 1106 26-27 0.04528119730992586 1106 28-29 -0.0713020420046675 1106 30-31 0.11798401552891136 1106 32-33 0.08429597095327779 1106 34-35 0.08284008091658279 1106 36-37 -0.06944331095960621 1106 38-39 -0.0806063570032407 1106 40-41 -0.05929146714768052 1106 42-43 0.02531223740931665 1106 44-45 0.020081760850835906 1106 46-47 0.024229459167735 1106 48-49 -0.2158043069844382 1106 50-51 -0.17544390970019208 1106 52-53 -0.24895836925386305 1106 54-55 -0.40938729665906237 1106 56-57 -0.17320547654745866 1106 58-59 -0.09223797431753411 1106 60-61 -0.24996582133022827 1106 62-63 -0.15373979499957358 1106 64-65 -0.27524536145713796 1106 66-67 -0.4369657763595143 1106 68-69 -0.3629082154796919 1106 70-71 -0.3930575048105922 1106 72-73 -0.14872088045595433 1106 74-75 -0.30379817769669515 1106 76-77 -0.29815943304361525 1106 78-79 -0.4178752768843488 1106 80-81 -0.6176663285588901 1106 82-83 -0.5065158594913726 1106 84-85 -0.5102959826790041 1106 86-87 -0.2662411601800443 1106 88-89 -0.2579459291076347 1106 90-91 -0.380915570301088 1106 92-93 -0.3136726168298374 1106 94-95 -0.2788192667256304 1106 96-97 -0.2492797241746345 1106 98-99 -0.2284629922098489 1106 100-101 -0.07046810859587538 1107 1 0.013914328546647425 1107 2 -0.015623315038290286 1107 3 -0.04046789641765258 1107 4 0.02125119294920097 1107 5 -0.0466666246106513 1107 6 0.010781187971986128 1107 7 -0.035489952440812544 1107 8 0.014363502382771287 1107 9 0.04821838013247515 1107 10-11 -0.031576954710565985 1107 12-13 0.0907036564955419 1107 14-15 0.04393155453657016 1107 16-17 0.003686385932574865 1107 18-19 0.03299440956627109 1107 20-21 0.016115687583152294 1107 22-23 0.04349667282489378 1107 24-25 -0.022125379127757583 1107 26-27 -0.06857699161989927 1107 28-29 0.14489091213908267 1107 30-31 0.17187824456393486 1107 32-33 0.19446467565809655 1107 34-35 0.24363337099552496 1107 36-37 0.2055499060353867 1107 38-39 0.22353809826264381 1107 40-41 0.1985328271453426 1107 42-43 0.28137608705088013 1107 44-45 0.3565439022729606 1107 46-47 0.3781558388623196 1107 48-49 0.34751806375814454 1107 50-51 0.4843097378313459 1107 52-53 0.5916301916983002 1107 54-55 0.44252535391973424 1107 56-57 0.5916298571842233 1107 58-59 0.5197899795047007 1107 60-61 0.4796955930165012 1107 62-63 0.5132240692274124 1107 64-65 0.6328169261328682 1107 66-67 0.5067536315037557 1107 68-69 0.5455044358832666 1107 70-71 0.709125132892261 1107 72-73 0.5318955653121549 1107 74-75 0.6187787996080765 1107 76-77 0.5471036769759436 1107 78-79 0.594997533538109 1107 80-81 0.45923522612154244 1107 82-83 0.577355330851514 1107 84-85 0.5742754647123256 1107 86-87 0.7419389086066843 1107 88-89 0.6058867745432686 1107 90-91 0.6163789124889014 1107 92-93 0.8677096419657211 1107 94-95 0.7125447524518478 1107 96-97 0.8617934334749506 1107 98-99 1.0072375706619425 1107 100-101 1.0815778495957211 1108 1 0.022339843643543134 1108 2 -0.00696474246658596 1108 3 0.07415148291691054 1108 4 0.040685941434695394 1108 5 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-0.4455469867788011 2316 52-53 -0.3097116642443112 2316 54-55 -0.4468509607078346 2316 56-57 -0.5651543022332177 2316 58-59 -0.5522414919284415 2316 60-61 -0.37110001541648074 2316 62-63 -0.5009446099718531 2316 64-65 -0.49635102688802846 2316 66-67 -0.36960782067309594 2316 68-69 -0.3047850963282386 2316 70-71 -0.4248932683883595 2316 72-73 -0.24626686213944282 2316 74-75 -0.21552885611088968 2316 76-77 -0.29794837638930716 2316 78-79 -0.23072799008411593 2316 80-81 -0.3164738030666001 2316 82-83 -0.4513968354483602 2316 84-85 -0.3910802714222328 2316 86-87 -0.3064975857057135 2316 88-89 -0.5242343371948159 2316 90-91 -0.3905498080112615 2316 92-93 -0.6775466143453528 2316 94-95 -0.5695691964426572 2316 96-97 -0.5266286642519908 2316 98-99 -0.5306151053384269 2316 100-101 -0.3621826115394491 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 33.0 6 60.0 7 171.0 8 401.0 9 705.0 10 1215.0 11 2077.0 12 3293.0 13 4800.0 14 6561.0 15 9021.0 16 11919.0 17 17797.0 18 17905.0 19 11684.0 20 9454.0 21 9184.0 22 9274.0 23 9944.0 24 11250.0 25 12971.0 26 15252.0 27 18761.0 28 22702.0 29 27437.0 30 33804.0 31 41998.0 32 53087.0 33 69571.0 34 98072.0 35 151802.0 36 251030.0 37 413625.0 38 361546.0 39 41422.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05207998184984 18.774098535471957 14.874329012765116 22.299492469913083 2 15.823650112312027 20.259432209832017 39.76645957052795 24.150458107328003 3 19.639383125567235 24.403848835587784 30.01450514481141 25.942262894033576 4 12.934273682213359 16.830214298735832 36.52513619762185 33.71037582142896 5 13.523875815357467 37.622625076721036 33.697251072380084 15.156248035541415 6 31.945268326469872 36.071631446424426 16.898801380235746 15.084298846869954 7 28.47357511828177 33.521445017764414 19.835562766344715 18.169417097609106 8 26.95891748064399 34.270540688198494 19.046326415383312 19.724215415774207 9 26.668022980373646 16.764780342899726 20.03164858978616 36.53554808694047 10-11 25.434966599937027 26.491544689335694 26.340074303639117 21.733414407088162 12-13 26.615784122840452 23.97246730714171 27.1942918438012 22.217456726216636 14-15 23.01244051622799 25.23657782651855 26.212891755679212 25.538089901574253 16-17 21.86377398645135 28.595953234634898 26.972520947605556 22.567751831308197 18-19 21.859225406564118 26.59229635505276 29.914701113868812 21.633777124514314 20-21 22.599975883605826 25.460425968670403 30.241529688024155 21.69806845969962 22-23 22.29697796435917 25.505515625080367 30.317907794224297 21.87959861633617 24-25 22.251778825546506 25.77596799542792 30.27569652807544 21.696556650950136 26-27 21.679327571621283 25.95516953609072 30.418467201682788 21.947035690605208 28-29 21.599923193262953 26.540956592478253 30.18576200826758 21.67335820599121 30-31 21.7410722194569 25.93112426400708 30.065142838202423 22.26266067833359 32-33 21.571223205772185 26.090492247722008 30.074541079601254 22.263743466904554 34-35 22.36005265405111 26.17352744989663 29.76700067960993 21.69941921644233 36-37 22.375834350360428 25.686403993166035 29.9785202734386 21.95924138303493 38-39 21.831718297606496 26.113537292621498 29.927721352108843 22.127023057663163 40-41 22.155155907510025 26.019535949748366 29.942329510543868 21.882978632197737 42-43 21.905718527239554 26.11636516389168 29.482584946341035 22.495331362527736 44-45 22.046598123262115 26.166974750991763 29.56692268764593 22.219504438100195 46-47 22.52842677102627 26.577152173875774 28.899412348622477 21.99500870647548 48-49 22.312722197997882 26.582352628544097 28.824777251954025 22.280147921503996 50-51 22.09081708286496 26.922896687538966 28.84352174790139 22.142764481694684 52-53 22.608205174509543 26.852347662395626 28.425056590661928 22.114390572432903 54-55 22.020668092696106 26.80917245907785 28.628045766287 22.54211368193905 56-57 22.27354734442497 27.040792709305446 28.36545137946346 22.320208566806127 58-59 22.456106162424486 26.573523285178645 28.3921680009738 22.578202551423068 60-61 21.97597847126063 26.854004950147072 28.861809061020764 22.308207517571535 62-63 21.933717633598825 26.919410668475585 28.880010652359893 22.266861045565697 64-65 21.718127356277435 27.058537117816588 28.615187499249934 22.608148026656043 66-67 21.73178569326347 27.054822507339217 28.381109891321927 22.83228190807539 68-69 22.0337133006466 26.778794789362593 28.587385100438983 22.600106809551825 70-71 22.115590677356366 26.85540507255777 28.18709292869605 22.841911321389812 72-73 21.998751890879603 26.870806419075492 28.347335509904582 22.78310618014032 74-75 21.826251095095742 27.607499398518843 27.85852134500902 22.707728161376394 76-77 21.993094253383298 27.036049437465103 28.11460087653378 22.85625543261782 78-79 22.1526796342766 27.160888923431592 28.039708614524585 22.646722827767228 80-81 22.331523841798738 27.337132903619572 27.75776967929196 22.573573575289725 82-83 22.187031833935148 27.27710687766041 28.012285649055503 22.523575639348937 84-85 22.10199871813665 27.411501851786763 27.815663135684638 22.670836294391947 86-87 22.228495797339647 27.396727936890375 27.65340796093823 22.721368304831746 88-89 22.050134111763516 27.52135687330289 27.396803091013982 23.031705923919613 90-91 22.020826433906805 27.39870737011624 27.587352811837174 22.993113384139782 92-93 22.23990001380125 27.14165825876229 27.732111736977323 22.886329990459135 94-95 21.965206100876248 26.98967395435144 27.90403961032022 23.141080334452095 96-97 22.01387378835801 26.702113585391636 28.058403980006247 23.225608646244115 98-99 21.925688360182345 27.10851291570063 27.444085111441176 23.52171361267585 100-101 21.882770322205314 26.69410525605953 27.870636689950608 23.55248773178455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1470.0 1 989.5 2 756.0 3 974.0 4 746.0 5 674.0 6 1324.5 7 2904.5 8 4693.0 9 5332.5 10 4259.0 11 2995.5 12 2737.5 13 2764.5 14 2913.5 15 3128.0 16 3236.0 17 3308.0 18 3397.5 19 3630.0 20 4042.0 21 4606.5 22 5309.0 23 6178.5 24 7343.0 25 8868.0 26 11057.0 27 13544.5 28 15533.5 29 17500.5 30 19898.5 31 23226.5 32 27893.0 33 30380.5 34 31126.0 35 32932.0 36 36319.0 37 39788.5 38 42089.5 39 43776.0 40 45689.5 41 49103.0 42 51372.0 43 52959.5 44 54882.5 45 58121.0 46 59940.0 47 63070.5 48 70184.0 49 69687.0 50 64450.5 51 62170.5 52 60896.5 53 60987.5 54 61619.5 55 58339.0 56 52734.0 57 47894.5 58 44258.5 59 39464.5 60 32244.5 61 26938.0 62 22842.0 63 17072.5 64 12190.0 65 9405.0 66 7132.0 67 5846.0 68 4470.5 69 3105.5 70 2277.5 71 1537.5 72 1113.0 73 914.5 74 715.0 75 541.0 76 355.5 77 184.5 78 105.5 79 56.0 80 29.5 81 15.5 82 6.5 83 4.0 84 3.0 85 2.5 86 2.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014686998348998514 3 0.007029185980259988 4 0.0 5 5.714785349804869E-5 6 5.714785349804869E-5 7 0.0 8 1.7144356049414607E-4 9 3.4288712098829214E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.857392674902434E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005543341789310723 26-27 0.020973262233783867 28-29 2.8573926749024346E-5 30-31 0.0 32-33 3.714610477373165E-4 34-35 0.017944425998387286 36-37 0.0032860015761377996 38-39 0.01705863426916753 40-41 0.014401259081508268 42-43 0.007972125562977791 44-45 0.0031717058691417025 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.000174872431704E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.8573926749024346E-5 84-85 0.0028859666016514585 86-87 1.7144356049414607E-4 88-89 2.8573926749024346E-5 90-91 2.000174872431704E-4 92-93 3.143131942392678E-4 94-95 0.0 96-97 2.8573926749024346E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1749847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.10702446982676 #Duplication Level Percentage of deduplicated Percentage of total 1 78.15044320477058 43.847888292178695 2 12.645945727125415 14.190527727118537 3 3.920002732792215 6.598190677516818 4 1.7051939401926814 3.8269343251276435 5 0.9023825423771422 2.53149996931494 6 0.5663529993190413 1.9065828954811934 7 0.37820981564981254 1.485415916697489 8 0.26412484016779547 1.1855407096306867 9 0.20435650470661113 1.0319251869127888 >10 1.145725828400335 11.808488336635081 >50 0.0757786813881415 2.8925050638106584 >100 0.03496063151229058 3.6414377338671478 >500 0.00428042448611072 1.7016612924024714 >1k 0.0019363825056215162 1.7260777503431233 >5k 1.019148687169219E-4 0.3752243489614912 >10k+ 2.038297374338438E-4 1.2500997740012376 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11831 0.6761162547354139 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10031 0.5732501184389264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6562 0.3750042146541955 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3574 0.204246428402026 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2589 0.14795579270644807 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2028 0.11589584689404274 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1976 0.1129241585121442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.7147853498048685E-5 2 0.0 0.0 0.0 0.0 5.7147853498048685E-5 3 0.0 0.0 0.0 0.0 5.7147853498048685E-5 4 0.0 0.0 0.0 0.0 5.7147853498048685E-5 5 0.0 0.0 0.0 0.0 5.7147853498048685E-5 6 0.0 0.0 0.0 0.0 5.7147853498048685E-5 7 0.0 0.0 0.0 0.0 5.7147853498048685E-5 8 0.0 0.0 0.0 0.0 5.7147853498048685E-5 9 0.0 0.0 0.0 0.0 5.7147853498048685E-5 10-11 0.0 0.0 0.0 0.0 5.7147853498048685E-5 12-13 0.0 0.0 0.0 0.0 5.7147853498048685E-5 14-15 0.0 0.0 0.0 5.7147853498048685E-5 8.572178024707303E-5 16-17 0.0 0.0 0.0 1.1429570699609737E-4 1.1429570699609737E-4 18-19 0.0 0.0 0.0 1.1429570699609737E-4 1.1429570699609737E-4 20-21 0.0 0.0 0.0 3.143131942392678E-4 1.7144356049414607E-4 22-23 0.0 0.0 0.0 4.571828279843895E-4 1.7144356049414607E-4 24-25 0.0 0.0 0.0 0.001171530996709998 1.7144356049414607E-4 26-27 0.0 0.0 0.0 0.0023716359201690204 1.7144356049414607E-4 28-29 0.0 0.0 0.0 0.005771933203302917 1.7144356049414607E-4 30-31 0.0 0.0 0.0 0.014972737616488756 1.7144356049414607E-4 32-33 0.0 0.0 0.0 0.03680321765274335 1.7144356049414607E-4 34-35 0.0 0.0 0.0 0.06992039875486257 1.7144356049414607E-4 36-37 0.0 0.0 0.0 0.12012478805289833 1.7144356049414607E-4 38-39 0.0 0.0 0.0 0.19455986723410673 1.7144356049414607E-4 40-41 0.0 0.0 0.0 0.2750526188861083 1.7144356049414607E-4 42-43 0.0 0.0 0.0 0.35617399692658847 1.7144356049414607E-4 44-45 0.0 0.0 0.0 0.45289673897203586 1.7144356049414607E-4 46-47 0.0 0.0 0.0 0.5659923410446741 2.2859141399219474E-4 48-49 0.0 0.0 0.0 0.6847170066868704 2.2859141399219474E-4 50-51 0.0 0.0 0.0 0.8092993273126164 2.2859141399219474E-4 52-53 0.0 0.0 0.0 0.926109539862628 2.2859141399219474E-4 54-55 0.0 0.0 0.0 1.0490631466636797 2.2859141399219474E-4 56-57 0.0 0.0 0.0 1.1965046086886453 2.2859141399219474E-4 58-59 0.0 0.0 0.0 1.351060978474118 2.2859141399219474E-4 60-61 0.0 0.0 0.0 1.5182755978094085 2.2859141399219474E-4 62-63 0.0 0.0 0.0 1.6805183538903687 2.857392674902434E-4 64-65 0.0 0.0 0.0 1.8387036123729674 2.857392674902434E-4 66-67 0.0 0.0 0.0 2.027148659282783 2.857392674902434E-4 68-69 0.0 0.0 0.0 2.2343096282132096 2.857392674902434E-4 70-71 0.0 0.0 0.0 2.4517286368465356 3.4288712098829214E-4 72-73 0.0 0.0 0.0 2.666404548511956 4.286089012353651E-4 74-75 0.0 0.0 0.0 2.885423697043227 4.8575675473341384E-4 76-77 0.0 0.0 0.0 3.1123863972107273 5.143306814824382E-4 78-79 0.0 0.0 0.0 3.371237599630139 5.143306814824382E-4 80-81 0.0 2.8573926749024343E-5 0.0 3.651262081770578 5.143306814824382E-4 82-83 0.0 5.7147853498048685E-5 0.0 3.9328295559554634 5.143306814824382E-4 84-85 0.0 5.7147853498048685E-5 0.0 4.213368368777385 5.143306814824382E-4 86-87 0.0 5.7147853498048685E-5 0.0 4.498907618780385 5.714785349804868E-4 88-89 0.0 5.7147853498048685E-5 0.0 4.797533727234438 6.286263884785355E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9810 0.0 29.879917 1 GTATCAA 13655 0.0 26.824146 1 TATAACG 110 9.469075E-5 25.913246 2 TACATGG 8310 0.0 24.868608 2 GTACATG 8425 0.0 24.867487 1 ACATGGG 8520 0.0 23.13714 3 ATCAACG 15965 0.0 22.73137 3 TCAACGC 15980 0.0 22.708088 4 TATCAAC 15985 0.0 22.706175 2 GAGTACT 9250 0.0 22.567375 12-13 CAACGCA 16120 0.0 22.481405 5 AACGCAG 16535 0.0 21.945885 6 GTACTTT 9660 0.0 21.289955 14-15 CATGGGA 3770 0.0 20.91369 4 AGAGTAC 13485 0.0 20.340658 10-11 AGTACTT 9605 0.0 20.249788 12-13 ACGCAGA 18070 0.0 20.055355 7 CATGGGG 4295 0.0 20.016096 4 CGCAGAG 18170 0.0 19.918839 8 TAAGGTG 955 0.0 19.89393 5 >>END_MODULE