FastQCFastQC Report
Thu 26 May 2016
SRR938032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795873
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119680.6664168345979922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101390.5645722164095123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62650.34885540347229455No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36480.20313240412879974No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.13692504982256543No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21100.11749160436177837No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18550.10329238203369615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91400.031.3613221
GTATCAA133900.030.581721
TATCAAC157550.025.841292
ATCAACG159150.025.522443
TCAACGC160750.025.2092294
CAACGCA161450.025.070475
GAGTACT92500.024.766312-13
AACGCAG165300.024.5372526
GTACATG88900.023.7807331
GTACTTT96900.023.56650514-15
TACATGG89500.023.3292262
TAAGGTG10400.022.8669195
ACATGGG89600.022.5075823
ACGCAGA180500.022.3850547
CGCAGAG181700.022.2365978
AGTACTT95850.021.3965912-13
GCAGAGT187300.021.2152739
CATGGGG43700.021.1150054
GTAAGCG2600.021.01028894-95
AGAGTAC140900.020.96369610-11
Produced by FastQC (version 0.11.5)