##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938032_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1795873 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.010003491338196 33.0 31.0 34.0 30.0 34.0 2 32.110614726097005 34.0 31.0 34.0 30.0 34.0 3 32.12624445047061 34.0 31.0 34.0 30.0 34.0 4 35.642682973684664 37.0 35.0 37.0 35.0 37.0 5 35.65953327434624 37.0 35.0 37.0 35.0 37.0 6 35.70381869987466 37.0 36.0 37.0 35.0 37.0 7 35.642317134897624 37.0 35.0 37.0 33.0 37.0 8 35.64024794626346 37.0 35.0 37.0 33.0 37.0 9 37.2760674056573 39.0 37.0 39.0 34.0 39.0 10-11 37.18613621341821 39.0 37.0 39.0 34.0 39.0 12-13 37.03217265363419 39.0 37.0 39.0 33.0 39.0 14-15 38.19682349475715 40.0 38.0 41.0 33.0 41.0 16-17 38.03123717545728 40.0 38.0 41.0 33.0 41.0 18-19 38.23374509221978 40.0 38.0 41.0 33.5 41.0 20-21 38.24633005786044 40.0 38.0 41.0 34.0 41.0 22-23 38.13647930560791 40.0 38.0 41.0 33.5 41.0 24-25 38.00377337372966 40.0 38.0 41.0 33.0 41.0 26-27 37.81832651863467 40.0 38.0 41.0 33.0 41.0 28-29 37.63764364183882 40.0 38.0 41.0 32.0 41.0 30-31 37.39213379787992 40.0 37.0 41.0 32.0 41.0 32-33 37.06982759916765 40.0 36.0 41.0 31.0 41.0 34-35 36.815273407418005 39.5 36.0 41.0 30.5 41.0 36-37 36.53508126688246 39.0 35.0 41.0 30.0 41.0 38-39 36.2321475404998 39.0 35.0 40.5 30.0 41.0 40-41 36.09533497079137 39.0 35.0 41.0 29.5 41.0 42-43 35.8468321534986 39.0 35.0 41.0 28.5 41.0 44-45 35.46907659951455 39.0 35.0 40.0 26.5 41.0 46-47 35.01146406232512 38.0 34.5 40.0 24.5 41.0 48-49 34.54329398571058 38.0 34.0 40.0 23.5 41.0 50-51 33.78043464097962 37.5 33.5 39.5 22.0 40.5 52-53 33.83347736727485 38.0 33.5 40.0 22.0 40.5 54-55 33.902928269426624 38.0 33.5 40.0 21.0 41.0 56-57 33.580031828531304 38.0 33.0 40.0 18.5 41.0 58-59 33.163855127840336 37.0 33.0 40.0 15.0 41.0 60-61 32.64726625991927 37.0 32.0 40.0 10.5 41.0 62-63 32.10146012552113 36.0 31.0 39.0 7.5 41.0 64-65 31.56280455243773 35.5 31.0 39.0 4.0 40.0 66-67 30.983002417208787 35.0 30.0 38.5 2.0 40.0 68-69 30.307162867307433 35.0 29.0 38.0 2.0 40.0 70-71 29.705137557054424 34.5 28.5 37.0 2.0 39.5 72-73 29.006970704498592 34.0 27.5 36.5 2.0 39.0 74-75 28.335933554321493 34.0 26.0 36.0 2.0 39.0 76-77 27.594624452842712 33.5 25.5 35.5 2.0 38.0 78-79 26.823013654083557 33.0 24.5 35.0 2.0 37.0 80-81 26.096093654729483 32.0 21.5 35.0 2.0 36.5 82-83 25.356060534347364 32.0 19.0 35.0 2.0 36.0 84-85 24.616566705997585 31.0 15.0 34.5 2.0 35.5 86-87 23.868098133888086 31.0 7.0 34.0 2.0 35.0 88-89 22.97999747198159 30.0 2.0 34.0 2.0 35.0 90-91 22.06878353870235 29.5 2.0 34.0 2.0 35.0 92-93 21.123977307972226 29.0 2.0 34.0 2.0 35.0 94-95 20.029895209739216 27.5 2.0 34.0 2.0 35.0 96-97 18.757379280160677 25.5 2.0 33.0 2.0 35.0 98-99 17.205167625995827 21.0 2.0 33.0 2.0 34.5 100-101 13.322002168304774 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2391434587216139 1101 2 0.13205328339791578 1101 3 0.0826317909273655 1101 4 -0.0488117467503244 1101 5 0.014690182221620773 1101 6 0.16736082226658056 1101 7 0.12920629362318437 1101 8 0.11306489468354641 1101 9 0.1636254512771913 1101 10-11 0.16399234313077926 1101 12-13 0.2776725534036686 1101 14-15 0.31378713106003886 1101 16-17 0.3396205728111781 1101 18-19 0.2202923784810622 1101 20-21 0.3307440183758814 1101 22-23 0.365925695517177 1101 24-25 0.3397068502328935 1101 26-27 0.3172118095848191 1101 28-29 0.3116425065703581 1101 30-31 0.37118857782332526 1101 32-33 0.42582686501333455 1101 34-35 0.38517187357145133 1101 36-37 0.4354121960875048 1101 38-39 0.3662769719870198 1101 40-41 0.4076398518936699 1101 42-43 0.47559674507723315 1101 44-45 0.48408530989512855 1101 46-47 0.3669268992738779 1101 48-49 0.3953353404335118 1101 50-51 0.5406910773486473 1101 52-53 0.6911942018499175 1101 54-55 0.5558374728528506 1101 56-57 0.6220874431634016 1101 58-59 0.5768486834529298 1101 60-61 0.5092630387989061 1101 62-63 0.3309331067174597 1101 64-65 0.22314755571007794 1101 66-67 0.28479619125399935 1101 68-69 0.34557879400496105 1101 70-71 0.4497730570209981 1101 72-73 0.3274977446954672 1101 74-75 0.19287322839286603 1101 76-77 0.25621898682781463 1101 78-79 0.20324535989376002 1101 80-81 0.18197442688817134 1101 82-83 -0.06924094428331884 1101 84-85 -0.3559853014650862 1101 86-87 -0.25568537004346936 1101 88-89 -0.20017887517668242 1101 90-91 -0.423130572795241 1101 92-93 -0.21132492102927714 1101 94-95 -0.360567858639957 1101 96-97 -0.4699843362591771 1101 98-99 -0.5431007876770799 1101 100-101 -0.6272232534793414 1102 1 0.09224836557080351 1102 2 0.10801829600168844 1102 3 0.0924469329373423 1102 4 0.10936097504677633 1102 5 0.12435462434733324 1102 6 0.09998578326827356 1102 7 0.10273476419178706 1102 8 0.20492164718525174 1102 9 0.22209845001240325 1102 10-11 0.12160188526459592 1102 12-13 0.07847922686612918 1102 14-15 0.15695599112248004 1102 16-17 0.09294395038135406 1102 18-19 0.16581425001482586 1102 20-21 0.23537882178831637 1102 22-23 0.282153121612879 1102 24-25 0.06654145490434615 1102 26-27 0.006472297422561724 1102 28-29 0.022265645378674037 1102 30-31 0.13577715270289303 1102 32-33 -0.05477664030287599 1102 34-35 0.09054598062049735 1102 36-37 0.18833215672427883 1102 38-39 -0.07312326813828918 1102 40-41 0.17781531018894015 1102 42-43 0.21980574712960532 1102 44-45 0.1046959451845666 1102 46-47 -0.20653428167104693 1102 48-49 -0.41983714637122205 1102 50-51 -0.16574771355546147 1102 52-53 -0.23134172671881714 1102 54-55 -0.13924930188892404 1102 56-57 -0.17194234713197432 1102 58-59 -0.24720599357691952 1102 60-61 -0.31233524615780794 1102 62-63 -0.2289221764804239 1102 64-65 -0.5188460618009465 1102 66-67 -0.4921735941811285 1102 68-69 -0.6325167893415404 1102 70-71 -0.8567117298296942 1102 72-73 -0.7611256571759668 1102 74-75 -0.59648358225542 1102 76-77 -0.8064684356267087 1102 78-79 -1.1303014448917459 1102 80-81 -0.9134282642155611 1102 82-83 -1.087916020415232 1102 84-85 -1.2057503892984975 1102 86-87 -1.0654570424753373 1102 88-89 -0.9868525221058597 1102 90-91 -1.0342673263235334 1102 92-93 -1.0000076324081206 1102 94-95 -1.3094046865156663 1102 96-97 -1.8050822510828688 1102 98-99 -1.8494815122074755 1102 100-101 -2.073050294283801 1103 1 0.2474255407751258 1103 2 0.2924103307315633 1103 3 0.21609643982588267 1103 4 0.15014809985063238 1103 5 0.25955472210638675 1103 6 0.3056082741285806 1103 7 0.30287736684720556 1103 8 0.3410716819203543 1103 9 0.28493405299256125 1103 10-11 0.3783053498439344 1103 12-13 0.233208900288858 1103 14-15 0.32372878764800106 1103 16-17 0.46488033823666086 1103 18-19 0.39443366124074686 1103 20-21 0.4673609426440706 1103 22-23 0.3564374831165864 1103 24-25 0.30594182867577757 1103 26-27 0.4059572312801194 1103 28-29 0.33429658596948997 1103 30-31 0.41865283838959755 1103 32-33 0.3733575631458308 1103 34-35 0.2881059654783087 1103 36-37 0.27100339295402165 1103 38-39 0.23842579057581048 1103 40-41 0.42095582724314795 1103 42-43 0.28984584852099005 1103 44-45 0.3449849696755223 1103 46-47 0.5074341641281634 1103 48-49 0.5022674610473246 1103 50-51 0.536594094097623 1103 52-53 0.7049527044157955 1103 54-55 0.6812432527651566 1103 56-57 0.6473272851168517 1103 58-59 0.6829130998199631 1103 60-61 0.528750611776907 1103 62-63 0.563367115577357 1103 64-65 0.28029010093947804 1103 66-67 0.17155133217259788 1103 68-69 0.19815133922656614 1103 70-71 0.08503998086030862 1103 72-73 -0.05967847938939386 1103 74-75 0.01431505614333517 1103 76-77 0.16530849585263852 1103 78-79 -0.05406881571507327 1103 80-81 -0.22321889317324306 1103 82-83 -0.4463462626416863 1103 84-85 -0.43957128469817874 1103 86-87 -0.588985172680232 1103 88-89 -0.45038551784160674 1103 90-91 -0.5735159418707418 1103 92-93 -0.8473213324649578 1103 94-95 -1.079806563668857 1103 96-97 -1.1401323469921216 1103 98-99 -1.3509620984777833 1103 100-101 -1.5837241927287362 1104 1 0.02814718822223483 1104 2 -0.009025454207019834 1104 3 -0.003243672230560435 1104 4 0.05668373755456457 1104 5 0.13091214658686567 1104 6 0.1244503575483833 1104 7 0.03235591900681101 1104 8 0.11194392374093098 1104 9 0.22089191818885467 1104 10-11 0.252898972310561 1104 12-13 0.28984607061662615 1104 14-15 0.21649329124981165 1104 16-17 0.12959416940099544 1104 18-19 0.33664158819654943 1104 20-21 0.19479031447581008 1104 22-23 0.3536718925475171 1104 24-25 0.4904569918690882 1104 26-27 0.37380152816052004 1104 28-29 0.23113104085540925 1104 30-31 0.4050422789967172 1104 32-33 0.404495469646875 1104 34-35 0.4990257868204182 1104 36-37 0.37597725633212065 1104 38-39 0.4222653048002627 1104 40-41 0.5011678352669477 1104 42-43 0.6185262208566868 1104 44-45 0.4974926525577672 1104 46-47 0.40638696998593105 1104 48-49 0.5620358382052046 1104 50-51 0.4735339354003969 1104 52-53 0.5211107198111975 1104 54-55 0.5252671561634088 1104 56-57 0.6254495985784487 1104 58-59 0.6850363915520958 1104 60-61 0.7433278309193909 1104 62-63 0.8668261418523215 1104 64-65 0.5693813963080068 1104 66-67 0.516053254128348 1104 68-69 0.716085063868114 1104 70-71 0.5688849299210723 1104 72-73 0.5365156529636899 1104 74-75 0.47149472195031095 1104 76-77 0.4248713373826938 1104 78-79 0.203417671305548 1104 80-81 0.11825063715808071 1104 82-83 0.11699057863144802 1104 84-85 0.2512124681057024 1104 86-87 0.11434070010507114 1104 88-89 0.23799579027408413 1104 90-91 0.21382632435718918 1104 92-93 0.11643448528027633 1104 94-95 -0.3224658290766165 1104 96-97 -0.4362370515840617 1104 98-99 -0.5757358116567666 1104 100-101 -1.0748864077703875 1105 1 0.07904792682789719 1105 2 0.12611202519275366 1105 3 0.1491185981644989 1105 4 0.05176837362280651 1105 5 0.05865623006865661 1105 6 0.043626748855920994 1105 7 0.07827290426806144 1105 8 0.0021959303863781088 1105 9 0.19341872350254619 1105 10-11 0.16365178962075788 1105 12-13 0.06057890487405615 1105 14-15 2.484704967997686E-4 1105 16-17 -0.005073758299708686 1105 18-19 0.0641754993370185 1105 20-21 -0.07030823299979261 1105 22-23 0.10092880988639052 1105 24-25 0.0876917243497175 1105 26-27 0.08099058985777674 1105 28-29 0.07680082341044425 1105 30-31 0.022749632359811756 1105 32-33 -0.020489518043028454 1105 34-35 -0.0194281787646986 1105 36-37 -0.07469863122901188 1105 38-39 -0.094613369045625 1105 40-41 0.004503737373333649 1105 42-43 -0.12003189679979442 1105 44-45 -0.23894572691339988 1105 46-47 -0.14130130106298822 1105 48-49 0.03579965787066186 1105 50-51 -0.049554497114257856 1105 52-53 -0.04737827449746845 1105 54-55 -0.0601090189668696 1105 56-57 -0.09521868274557477 1105 58-59 -0.27262906078548355 1105 60-61 -0.21629505985104203 1105 62-63 -0.2653779321550296 1105 64-65 -0.41693972034097726 1105 66-67 -0.28384966626637365 1105 68-69 -0.3446295554530643 1105 70-71 -0.2813000550999796 1105 72-73 -0.6624198074471792 1105 74-75 -0.8867508727706941 1105 76-77 -0.7606581301139848 1105 78-79 -0.8552893017456888 1105 80-81 -0.7146651871511835 1105 82-83 -1.0711475279492824 1105 84-85 -1.1385022646418186 1105 86-87 -0.9018587501107724 1105 88-89 -0.7510192020195952 1105 90-91 -1.1220427549545136 1105 92-93 -1.3063059952470901 1105 94-95 -1.7947476001288827 1105 96-97 -1.8443790613654194 1105 98-99 -2.0445861022283705 1105 100-101 -1.7048171176191307 1106 1 0.28748465139364754 1106 2 0.33394954012704403 1106 3 0.3252733633515206 1106 4 0.20316254695246272 1106 5 0.29807540110072495 1106 6 0.280546149646284 1106 7 0.39814423962326373 1106 8 0.22401010187796544 1106 9 0.47534685510809993 1106 10-11 0.4172277477128432 1106 12-13 0.4652200224184 1106 14-15 0.5046405300501462 1106 16-17 0.171146258242878 1106 18-19 0.5613035206585906 1106 20-21 0.5533651426090671 1106 22-23 0.6097340667561681 1106 24-25 0.65101678638446 1106 26-27 0.5928893124016668 1106 28-29 0.49136749926743306 1106 30-31 0.4707516542327923 1106 32-33 0.5080381908072624 1106 34-35 0.4616627136165192 1106 36-37 0.4476301988417575 1106 38-39 0.30592058923026855 1106 40-41 0.3962260055472129 1106 42-43 0.4152562201012273 1106 44-45 0.45664842803559225 1106 46-47 0.5519392099194533 1106 48-49 0.5463464826110425 1106 50-51 0.2936189244462568 1106 52-53 0.3684382864803908 1106 54-55 0.30681773408892354 1106 56-57 0.23787386389536636 1106 58-59 0.22225743861714164 1106 60-61 0.70937905098436 1106 62-63 0.5467762816120896 1106 64-65 0.4186810865701496 1106 66-67 0.23843596924507082 1106 68-69 0.067897785783952 1106 70-71 -0.0021474036892676907 1106 72-73 0.02638269232306456 1106 74-75 0.21382719909770032 1106 76-77 -0.23338582058361013 1106 78-79 -0.02197894424178415 1106 80-81 -0.10655747646282876 1106 82-83 -0.2900400929708802 1106 84-85 -0.26737369190034244 1106 86-87 -0.08441090524357264 1106 88-89 -0.39098887392475135 1106 90-91 -0.5112919048488251 1106 92-93 -0.4843107742196118 1106 94-95 -0.3633304509697943 1106 96-97 -0.49466947584938126 1106 98-99 -0.9064976912367335 1106 100-101 -0.9655523441608072 1107 1 -0.10580647304463042 1107 2 -0.16111201915195394 1107 3 -0.07798986312314327 1107 4 -0.006779151315186027 1107 5 -0.09093781284491342 1107 6 -0.16402911638668627 1107 7 -0.04004289390273641 1107 8 -0.005066592463926156 1107 9 -0.03177230427781552 1107 10-11 0.04361788486390594 1107 12-13 -0.017129086350244904 1107 14-15 -0.01586514142209694 1107 16-17 -0.09532857512107995 1107 18-19 -0.07827065952550782 1107 20-21 -0.10223683530642802 1107 22-23 0.04389637610616859 1107 24-25 -0.008272901392224696 1107 26-27 -0.08742959306265874 1107 28-29 -0.034500762204977775 1107 30-31 -0.14502506182607533 1107 32-33 -0.18083954260848145 1107 34-35 -0.2051428860226423 1107 36-37 -0.1958548749124418 1107 38-39 -0.3137685238817767 1107 40-41 -0.23085222568847996 1107 42-43 -0.11686937085114124 1107 44-45 -0.07915384723545316 1107 46-47 -0.21313571365349304 1107 48-49 -0.3172618767548272 1107 50-51 -0.2986159196091336 1107 52-53 -0.24777427261484775 1107 54-55 -0.33030042297494333 1107 56-57 -0.560324963387842 1107 58-59 -0.3941281880847143 1107 60-61 -0.4218309187789302 1107 62-63 -0.29491948023619585 1107 64-65 -0.4881245130690104 1107 66-67 -0.557379169158903 1107 68-69 -0.6824690640849091 1107 70-71 -0.8244417188527287 1107 72-73 -0.693140202383006 1107 74-75 -0.763201083089367 1107 76-77 -0.8642715391649922 1107 78-79 -0.9510922233383532 1107 80-81 -0.8731671221419823 1107 82-83 -1.0197059856221848 1107 84-85 -1.1651543838366614 1107 86-87 -1.099090746876687 1107 88-89 -0.8583698064183629 1107 90-91 -0.9747586026988451 1107 92-93 -1.1032601736616492 1107 94-95 -1.2465824848482825 1107 96-97 -1.1023454251318832 1107 98-99 -1.1703796845211762 1107 100-101 -1.7174465657742122 1108 1 0.018011917735897498 1108 2 0.014597711501146193 1108 3 0.05738159258612541 1108 4 0.09965111699543883 1108 5 0.10251226459342888 1108 6 0.11026771689657267 1108 7 0.08916114626050131 1108 8 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48-49 -0.35747565063736886 2316 50-51 -0.36473056066832044 2316 52-53 -0.45970372786664626 2316 54-55 -0.4461284114742696 2316 56-57 -0.357583314926849 2316 58-59 -0.18650880795154734 2316 60-61 -0.26771453835091563 2316 62-63 -0.16235895353661434 2316 64-65 -0.04704271038754726 2316 66-67 -0.020704270680919734 2316 68-69 0.06371091043324029 2316 70-71 0.09464013593188625 2316 72-73 0.2174647858365688 2316 74-75 0.22332672136895937 2316 76-77 -0.03322733596951011 2316 78-79 0.16807292345010794 2316 80-81 0.2520372427388331 2316 82-83 0.12349843623124102 2316 84-85 0.01686201794390385 2316 86-87 -0.08282226760461242 2316 88-89 0.2873688008253872 2316 90-91 0.32578955169302404 2316 92-93 0.10315438191427617 2316 94-95 0.3005919294842734 2316 96-97 0.46799139740842577 2316 98-99 -0.01713481455141519 2316 100-101 0.056220256958441794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5594.0 3 1631.0 4 1534.0 5 1728.0 6 2094.0 7 2704.0 8 3341.0 9 4359.0 10 5593.0 11 6898.0 12 9033.0 13 11089.0 14 13161.0 15 16285.0 16 19830.0 17 25986.0 18 25136.0 19 20020.0 20 19838.0 21 21083.0 22 22772.0 23 25507.0 24 28140.0 25 32070.0 26 36050.0 27 40807.0 28 47021.0 29 54506.0 30 64129.0 31 75919.0 32 91707.0 33 113862.0 34 144996.0 35 190730.0 36 235202.0 37 245105.0 38 123065.0 39 7347.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84027202690155 18.36421379596867 14.525835027227785 22.269679149901993 2 15.645503556340715 19.99328119855395 40.35163956025835 24.009575684846986 3 19.63420435236258 24.498616151020503 29.787690854328076 26.07948864228884 4 12.693000116526267 16.67920206825763 36.897067162061276 33.73073065315482 5 13.476484591313739 37.53009127664002 33.77492310859415 15.218501023452088 6 31.668588727517605 36.05233807962409 17.09864217140989 15.180431021448415 7 28.39385740491923 33.59045536255601 19.971800239050978 18.043886993473784 8 26.825449187884352 34.17277211502241 19.18752772248759 19.814250974605642 9 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30.056977674452312 22.25836755060114 34-35 22.4324099126276 26.208514970183366 29.727070283881574 21.632004833307462 36-37 22.385295587154676 25.643942254399093 29.975113113976143 21.995649044470085 38-39 21.85256538108837 26.172408183458145 29.882759181654183 22.092267253799296 40-41 22.19248245723756 26.100432436859567 29.848088549964523 21.858996555938354 42-43 21.959380777215525 26.099307653476835 29.457127001514404 22.48418456779324 44-45 22.025131215538863 26.2594853431881 29.499013446320006 22.216369994953034 46-47 22.563599572567192 26.595713860701032 28.828064150577582 22.012622416154194 48-49 22.33815010627651 26.633832502869648 28.713069573721306 22.314947817132534 50-51 22.201525070852227 27.00078498751764 28.675137177822236 22.1225527638079 52-53 22.61952504127315 26.868375965546665 28.389327799054897 22.12277119412529 54-55 22.08384704051736 26.88351098827327 28.509620554007082 22.523021417202287 56-57 22.349069790174696 27.04814510844395 28.25708508169187 22.345700019689485 58-59 22.536382385810953 26.535909952411245 28.319680305209804 22.608027356567995 60-61 22.113318765008614 26.747544450008604 28.838849187759262 22.300287597223516 62-63 22.01390571855962 26.845307237826226 28.90929805648641 22.23148898712774 64-65 21.820182676712687 27.05479270819494 28.542338098146747 22.58268651694563 66-67 21.84394603046746 27.04088936869729 28.366480778047205 22.748683822788042 68-69 22.174743735969347 26.692078088582633 28.562219073499033 22.570959101948983 70-71 22.239310072995547 26.820717309039555 28.168704534470585 22.771268083494313 72-73 22.142961004000604 26.82270934646032 28.356069903881888 22.678259745657193 74-75 22.08558455427079 27.46982542279503 27.892383701076856 22.55220632185733 76-77 22.268291059628744 26.99994959625486 27.947289378482836 22.78446996563356 78-79 22.38903232472152 27.194060002066365 27.88141948840426 22.535488184807857 80-81 22.641009195595004 27.24782990474668 27.575506002799976 22.535654896858336 82-83 22.513903315909513 27.250137153391798 27.770150409232304 22.465809121466386 84-85 22.505698960124263 27.355482001422445 27.572134382398694 22.566684656054598 86-87 22.645043519669834 27.242101921381934 27.487221187973503 22.625633370974725 88-89 22.53246187023944 27.293740470519868 27.193782788167443 22.980014871073255 90-91 22.58641996986526 27.240497607783524 27.294819289130146 22.878263133221065 92-93 22.845996653033975 26.924575123114963 27.402155856271047 22.827272367580015 94-95 22.669617082170205 26.811213622137476 27.47034840777588 23.04882088791644 96-97 22.783494010678144 26.49972641575667 27.57612161181795 23.140657961747237 98-99 22.687548888735368 26.78824129808367 26.946913797598963 23.577296015581997 100-101 23.58628349236979 25.47621032231122 27.215765949480964 23.721740235838023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1537.0 1 1059.0 2 745.5 3 970.0 4 825.0 5 713.0 6 1263.0 7 2719.5 8 4485.0 9 5234.0 10 4232.5 11 2994.0 12 2712.0 13 2798.5 14 3012.0 15 3168.5 16 3311.0 17 3501.5 18 3652.5 19 3903.0 20 4318.0 21 4919.5 22 5699.0 23 6658.5 24 7962.0 25 9675.5 26 11878.0 27 14372.0 28 16506.0 29 18389.0 30 20536.0 31 23842.0 32 28603.0 33 31096.0 34 32156.0 35 34113.5 36 37341.5 37 40995.0 38 43244.5 39 44790.5 40 47011.0 41 49809.5 42 51587.0 43 53445.5 44 55591.0 45 58725.5 46 60833.5 47 63665.5 48 69106.5 49 69430.5 50 65384.5 51 62680.0 52 61440.5 53 61479.0 54 61798.0 55 58840.5 56 53882.0 57 49596.5 58 45909.0 59 41086.5 60 34101.0 61 28531.0 62 24302.5 63 18900.5 64 14048.5 65 10916.0 66 8464.5 67 6734.0 68 5262.5 69 3842.0 70 2850.5 71 2056.5 72 1508.0 73 1141.5 74 851.0 75 659.0 76 447.0 77 292.5 78 188.0 79 113.0 80 61.5 81 38.5 82 25.5 83 19.0 84 15.5 85 7.5 86 6.0 87 6.0 88 3.5 89 1.5 90 2.5 91 3.5 92 2.0 93 1.0 94 2.0 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14856284380911122 2 0.05067173458256793 3 0.1495651418557994 4 0.1272918519293959 5 0.1898797966225897 6 0.1963390507012467 7 0.16426551320722568 8 0.07244387548562733 9 0.12099964752518692 10-11 0.10176109335125591 12-13 0.1495929834682074 14-15 0.13670231692330137 16-17 0.13383463084527691 18-19 0.1311896776665165 20-21 0.1324982334496927 22-23 0.19182870949115 24-25 0.21042690657969687 26-27 0.1439968193741985 28-29 0.1247582652002675 30-31 0.09744564342801523 32-33 0.12183489589742703 34-35 0.09223926190771842 36-37 0.08655957297648553 38-39 0.05206381520296814 40-41 0.05816112832032109 42-43 0.06164132987132164 44-45 0.04092717023976639 46-47 0.05406841129634445 48-49 0.04407327244187089 50-51 0.052342231327048176 52-53 0.028788227229876503 54-55 0.05089446748183196 56-57 0.02775808757078034 58-59 0.05072741780738393 60-61 0.036416829029669695 62-63 0.05167403262925608 64-65 0.04089932862735839 66-67 0.0396464560689982 68-69 0.06359024273988194 70-71 0.05014274394681584 72-73 0.04978080298551178 74-75 0.04098285346458241 76-77 0.020686318019147235 78-79 0.025140976004427933 80-81 0.027980820470044375 82-83 0.02438925246941181 84-85 0.02060279318192322 86-87 0.02341479603513166 88-89 0.06200327083262569 90-91 0.10746862389489681 92-93 0.07862471344020429 94-95 0.07369674804398751 96-97 0.06565052205807426 98-99 0.054625243544504536 100-101 0.12871177416220414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1795873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.05853090211439 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58327082617627 46.20487788552894 2 11.939657133859425 13.863979053336559 3 3.6251775929284538 6.31417455914068 4 1.5773814749319572 3.6632180442703923 5 0.8682614639225166 2.520499251713025 6 0.49785544892836114 1.7342853579835977 7 0.3295725168264735 1.3394147306860198 8 0.24684818403501962 1.1465314336742405 9 0.18405768203186315 0.9617506758016623 >10 1.042353425389799 11.175951995790689 >50 0.06739063788601934 2.694718614670332 >100 0.03221271357124108 3.501246067437568 >500 0.004038028702090041 1.7340336120532052 >1k 0.0016344401889412073 1.561607092573651 >5k 9.614354052595339E-5 0.34970933782434666 >10k+ 1.9228708105190677E-4 1.2340022875152168 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11968 0.6664168345979922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10139 0.5645722164095123 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6265 0.34885540347229455 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3648 0.20313240412879974 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.13692504982256543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2110 0.11749160436177837 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1855 0.10329238203369615 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.568322481600871E-5 0.0 10-11 0.0 0.0 0.0 8.352483722401307E-5 0.0 12-13 0.0 0.0 0.0 1.1136644963201741E-4 0.0 14-15 0.0 0.0 0.0 1.1136644963201741E-4 0.0 16-17 0.0 0.0 0.0 2.2273289926403483E-4 0.0 18-19 0.0 0.0 0.0 2.2273289926403483E-4 0.0 20-21 0.0 0.0 0.0 2.2273289926403483E-4 5.568322481600871E-5 22-23 0.0 0.0 0.0 5.846738605680914E-4 5.568322481600871E-5 24-25 0.0 0.0 0.0 0.0010579812715041654 5.568322481600871E-5 26-27 0.0 0.0 0.0 0.0019489128685603045 5.568322481600871E-5 28-29 0.0 0.0 0.0 0.005429114419560848 5.568322481600871E-5 30-31 0.0 0.0 0.0 0.015340728436810398 5.568322481600871E-5 32-33 0.0 0.0 0.0 0.03638898741726169 5.568322481600871E-5 34-35 0.0 0.0 0.0 0.06798921750034663 5.568322481600871E-5 36-37 0.0 0.0 0.0 0.11668419760194623 1.1136644963201741E-4 38-39 0.0 0.0 0.0 0.1899633214598137 1.1136644963201741E-4 40-41 0.0 0.0 0.0 0.2677806281401859 1.1136644963201741E-4 42-43 0.0 0.0 0.0 0.3481593631620944 1.1136644963201741E-4 44-45 0.0 0.0 0.0 0.4442686091945255 1.1136644963201741E-4 46-47 0.0 0.0 0.0 0.5542151365937347 1.1136644963201741E-4 48-49 0.0 0.0 0.0 0.6659156855746482 1.1136644963201741E-4 50-51 0.0 0.0 0.0 0.7901449601391635 1.1136644963201741E-4 52-53 0.0 0.0 0.0 0.9066899496790697 1.1136644963201741E-4 54-55 0.0 0.0 0.0 1.0310027490808091 1.670496744480261E-4 56-57 0.0 0.0 0.0 1.1754171926411279 1.670496744480261E-4 58-59 0.0 0.0 0.0 1.330327924079264 1.670496744480261E-4 60-61 0.0 0.0 0.0 1.4947326453485297 1.670496744480261E-4 62-63 0.0 0.0 0.0 1.6553509073303068 1.670496744480261E-4 64-65 0.0 0.0 0.0 1.8155793867383718 1.670496744480261E-4 66-67 0.0 0.0 0.0 2.003120487918689 1.9489128685603045E-4 68-69 0.0 0.0 0.0 2.206809724295649 2.2273289926403483E-4 70-71 0.0 0.0 0.0 2.4199372672789226 2.2273289926403483E-4 72-73 0.0 0.0 0.0 2.6415008188218208 2.2273289926403483E-4 74-75 0.0 0.0 0.0 2.848753781587005 2.2273289926403483E-4 76-77 0.0 0.0 0.0 3.070762798928432 2.2273289926403483E-4 78-79 0.0 0.0 0.0 3.325179453112776 2.2273289926403483E-4 80-81 0.0 0.0 0.0 3.5929600812529614 2.2273289926403483E-4 82-83 0.0 0.0 0.0 3.864415802231004 2.2273289926403483E-4 84-85 0.0 0.0 0.0 4.134535125813462 2.2273289926403483E-4 86-87 0.0 0.0 0.0 4.411058020249762 2.2273289926403483E-4 88-89 0.0 0.0 0.0 4.707905291743904 2.2273289926403483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9140 0.0 31.361322 1 GTATCAA 13390 0.0 30.58172 1 TATCAAC 15755 0.0 25.84129 2 ATCAACG 15915 0.0 25.52244 3 TCAACGC 16075 0.0 25.209229 4 CAACGCA 16145 0.0 25.07047 5 GAGTACT 9250 0.0 24.7663 12-13 AACGCAG 16530 0.0 24.537252 6 GTACATG 8890 0.0 23.780733 1 GTACTTT 9690 0.0 23.566505 14-15 TACATGG 8950 0.0 23.329226 2 TAAGGTG 1040 0.0 22.866919 5 ACATGGG 8960 0.0 22.507582 3 ACGCAGA 18050 0.0 22.385054 7 CGCAGAG 18170 0.0 22.236597 8 AGTACTT 9585 0.0 21.39659 12-13 GCAGAGT 18730 0.0 21.215273 9 CATGGGG 4370 0.0 21.115005 4 GTAAGCG 260 0.0 21.010288 94-95 AGAGTAC 14090 0.0 20.963696 10-11 >>END_MODULE