FastQCFastQC Report
Thu 26 May 2016
SRR938032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795873
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114800.63924342088778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100320.5586141113541992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.3578760858924879No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36650.2040790189506719No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.14550026644423075No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20730.11543132504358605No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18450.10273554978553606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18400.10245713366145602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97600.029.6958831
GTATCAA133400.026.7841721
TAAGGTG9500.025.9935865
GTAAGGT9300.024.510084
GGTAAGG9400.023.7481063
TATACCG1000.001656384223.7441395
AGGTAAG9200.022.2030262
GTACATG85350.022.0448881
GAGTACT91350.021.99148412-13
TATCAAC161000.021.9522272
ATCAACG161800.021.722623
TCAACGC162600.021.6413364
CCGTCGT1100.002881182421.5867849
CAACGCA163050.021.581615
TACATGG85500.021.5018772
GTATAGG5350.021.3144281
AACGCAG166800.021.0394736
ACATGGG82500.020.7831923
GTACTTT96450.020.7536214-15
AGAGTAC141250.020.35862410-11
Produced by FastQC (version 0.11.5)