##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938032_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1795873 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53282720994191 34.0 31.0 34.0 31.0 34.0 2 32.74048777391274 34.0 31.0 34.0 31.0 34.0 3 32.7871831694112 34.0 31.0 34.0 31.0 34.0 4 36.239696236871985 37.0 35.0 37.0 35.0 37.0 5 36.22003838801519 37.0 37.0 37.0 35.0 37.0 6 36.261933889534504 37.0 37.0 37.0 35.0 37.0 7 36.22253466698369 37.0 37.0 37.0 35.0 37.0 8 36.21189248905685 37.0 37.0 37.0 35.0 37.0 9 37.90308891553022 39.0 38.0 39.0 35.0 39.0 10-11 37.88080309687823 39.0 38.0 39.0 35.0 39.0 12-13 37.80025898267862 39.0 38.0 39.0 35.0 39.0 14-15 39.061539429569905 40.0 38.5 41.0 36.0 41.0 16-17 38.95290813994085 40.0 38.0 41.0 36.0 41.0 18-19 39.22412665038118 40.5 39.0 41.0 36.0 41.0 20-21 39.21725589727113 41.0 39.0 41.0 36.0 41.0 22-23 39.11938232826041 40.0 39.0 41.0 35.5 41.0 24-25 39.01002910562161 40.0 38.5 41.0 35.0 41.0 26-27 38.831914895986515 40.0 38.0 41.0 35.0 41.0 28-29 38.71657878925737 40.0 38.0 41.0 35.0 41.0 30-31 38.46281251513888 40.0 38.0 41.0 34.0 41.0 32-33 38.22397407834518 40.0 38.0 41.0 34.0 41.0 34-35 38.04597485456934 40.0 37.0 41.0 34.0 41.0 36-37 38.02133335709151 40.0 38.0 41.0 33.5 41.0 38-39 37.86906173209353 40.0 37.0 41.0 33.0 41.0 40-41 37.68839110560714 40.0 37.0 41.0 33.0 41.0 42-43 37.4416679241795 40.0 37.0 41.0 33.0 41.0 44-45 37.126829124331174 40.0 36.0 41.0 32.0 41.0 46-47 36.668838776461364 40.0 36.0 41.0 31.0 41.0 48-49 36.21450236180398 39.0 35.0 41.0 30.0 41.0 50-51 35.80862009730087 39.0 35.0 40.5 28.5 41.0 52-53 35.294413079321316 38.5 35.0 40.0 27.0 41.0 54-55 34.978739587933 38.0 34.5 40.0 26.0 41.0 56-57 34.62574942660199 38.0 34.0 40.0 26.0 41.0 58-59 34.18458432194259 37.5 33.5 40.0 24.5 41.0 60-61 33.69885760295968 37.0 33.0 40.0 23.0 41.0 62-63 33.24042819286219 36.0 33.0 39.0 22.0 41.0 64-65 32.74982612913051 36.0 32.0 39.0 20.5 40.0 66-67 32.202547730268236 35.0 31.0 38.5 18.5 40.0 68-69 31.619211102344096 35.0 31.0 38.0 15.0 40.0 70-71 31.00284123654624 35.0 30.0 37.0 9.5 39.5 72-73 30.42124860722334 34.0 29.5 37.0 7.0 39.0 74-75 29.836458647131508 34.0 29.0 36.0 4.5 39.0 76-77 28.483788942759315 32.5 27.0 35.0 2.0 37.0 78-79 29.164365464595768 34.0 29.0 35.0 2.0 37.0 80-81 28.82939049698949 34.0 29.0 35.0 2.0 37.0 82-83 28.267829907794148 33.5 27.5 35.0 2.0 36.0 84-85 27.67862733055177 33.0 26.0 35.0 2.0 36.0 86-87 27.041968446543827 33.0 25.5 35.0 2.0 35.5 88-89 26.296453869510817 32.0 24.5 35.0 2.0 35.0 90-91 25.75585550871359 32.0 23.0 34.0 2.0 35.0 92-93 24.95302952937095 31.5 19.0 34.0 2.0 35.0 94-95 24.12527222136532 31.0 11.5 34.0 2.0 35.0 96-97 23.065358185127792 31.0 2.0 34.0 2.0 35.0 98-99 21.626822999176447 30.0 2.0 34.0 2.0 35.0 100-101 17.2784144535833 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11581363986105941 1101 2 0.07450890133693377 1101 3 0.06377515431186254 1101 4 0.07359138769454887 1101 5 0.05228946475511975 1101 6 0.03111397219235812 1101 7 0.015496413788149255 1101 8 0.08648062917571053 1101 9 -0.043303752315367205 1101 10-11 0.10552455541738937 1101 12-13 0.21610287331067468 1101 14-15 0.13353415683257452 1101 16-17 0.056576518639523954 1101 18-19 0.20751488538896723 1101 20-21 0.2051014363311765 1101 22-23 0.22761785049372918 1101 24-25 0.1953292880991242 1101 26-27 0.2270201821193325 1101 28-29 0.36084306535054367 1101 30-31 0.253131196710882 1101 32-33 0.17098288372361026 1101 34-35 0.2696340905664911 1101 36-37 0.39460790983757477 1101 38-39 0.3432850335701474 1101 40-41 0.2593118057773154 1101 42-43 0.5045904757959576 1101 44-45 0.4013236038172252 1101 46-47 0.36882895601246446 1101 48-49 0.5482912499214478 1101 50-51 0.44972270973939743 1101 52-53 0.17772936247779825 1101 54-55 0.12500843747953638 1101 56-57 0.2899229799880487 1101 58-59 0.37911198254331424 1101 60-61 0.23248694796970426 1101 62-63 0.3161460911644127 1101 64-65 0.2711283435546008 1101 66-67 0.03911053787188479 1101 68-69 0.13137355847613819 1101 70-71 0.2244498066238485 1101 72-73 0.15555386607068655 1101 74-75 0.01522464325063666 1101 76-77 -0.10626087411522533 1101 78-79 -0.155776841316694 1101 80-81 -0.13254060237861154 1101 82-83 0.04090300480228848 1101 84-85 0.10009476207286738 1101 86-87 -0.11495079663600904 1101 88-89 -0.18485251445871853 1101 90-91 -0.07802015317230016 1101 92-93 -0.14427909414097329 1101 94-95 -0.2560585653612648 1101 96-97 -0.286171641953743 1101 98-99 -0.2178814785901544 1101 100-101 -0.644815644533903 1102 1 0.029809640980680285 1102 2 0.04971520137807772 1102 3 -0.017565489182253202 1102 4 -0.013078690237904311 1102 5 0.02359433005958067 1102 6 -0.003510108189466621 1102 7 0.05841540397862133 1102 8 0.11770894952564959 1102 9 -0.017229624654362397 1102 10-11 0.11902939842564564 1102 12-13 0.07963446374383665 1102 14-15 0.03853428114594237 1102 16-17 0.11740605774169666 1102 18-19 0.07171014880899662 1102 20-21 0.044577788319266176 1102 22-23 0.0600889436252956 1102 24-25 0.07418450633324625 1102 26-27 0.02854907615542146 1102 28-29 -0.035003584885373584 1102 30-31 -0.08218186044359044 1102 32-33 0.01910883963327592 1102 34-35 0.012692191427142063 1102 36-37 0.14040270936823163 1102 38-39 0.14339354711108143 1102 40-41 0.05094856371673018 1102 42-43 0.11952440123520347 1102 44-45 -0.034513654744159794 1102 46-47 -0.030320018723045905 1102 48-49 0.08713576918560761 1102 50-51 0.06352799009010113 1102 52-53 0.026675385612925595 1102 54-55 0.150030513127831 1102 56-57 0.24234504553086822 1102 58-59 0.15380041714927017 1102 60-61 -0.10711933153968545 1102 62-63 -0.08099321760512623 1102 64-65 -0.3034865456581315 1102 66-67 -0.3468040756859736 1102 68-69 -0.1630542351059283 1102 70-71 -0.14391877690150068 1102 72-73 -0.33328118933698647 1102 74-75 -0.552428496784767 1102 76-77 -0.4718991751444612 1102 78-79 -0.38210797684313036 1102 80-81 -0.4358150967239567 1102 82-83 -0.42757453337594953 1102 84-85 -0.6059285815717921 1102 86-87 -0.6067962406312297 1102 88-89 -0.718789061707021 1102 90-91 -0.8488013383839821 1102 92-93 -0.9230574385916661 1102 94-95 -0.9760418993495854 1102 96-97 -0.9638204590919841 1102 98-99 -0.7441587182559033 1102 100-101 -1.1200357794499958 1103 1 0.13423992730334788 1103 2 0.0392860742625345 1103 3 0.06789670074758902 1103 4 0.01466118846762754 1103 5 0.008572844368607946 1103 6 0.022388193246989374 1103 7 8.571455995038946E-4 1103 8 0.110114311774133 1103 9 0.19616351509661456 1103 10-11 0.10530426653874514 1103 12-13 0.13106278853259568 1103 14-15 0.17069435636361163 1103 16-17 0.12296554334073306 1103 18-19 0.0607217846027055 1103 20-21 0.10144265249733309 1103 22-23 0.16872761517842605 1103 24-25 0.14199345166030497 1103 26-27 0.1643306459580387 1103 28-29 0.14025007108273257 1103 30-31 0.06681885593295789 1103 32-33 0.12968008359853656 1103 34-35 0.1182162879500126 1103 36-37 0.062388107543917215 1103 38-39 0.1132055814149382 1103 40-41 0.07277469236480272 1103 42-43 0.041057252724037596 1103 44-45 0.09213607598869089 1103 46-47 -0.01373367815905624 1103 48-49 -0.11746989299183497 1103 50-51 -0.05618649069385384 1103 52-53 -0.18881456624373527 1103 54-55 -0.3005154016760514 1103 56-57 -0.28715980262308705 1103 58-59 -0.012724618473363591 1103 60-61 -0.2107282325463089 1103 62-63 -0.1808102183379532 1103 64-65 -0.3453190869413305 1103 66-67 -0.3821400869297591 1103 68-69 -0.37641803966704046 1103 70-71 -0.3120195046010572 1103 72-73 -0.5876692292492898 1103 74-75 -0.6305120456476594 1103 76-77 -0.5534420342305388 1103 78-79 -0.6125045026597817 1103 80-81 -0.5691418529956138 1103 82-83 -0.4814664535677906 1103 84-85 -0.49684521096221346 1103 86-87 -0.8112044041100788 1103 88-89 -0.9550933584341479 1103 90-91 -1.129031064684792 1103 92-93 -0.9721933210595708 1103 94-95 -1.1028611015295837 1103 96-97 -1.2581955392032143 1103 98-99 -1.6103947368615117 1103 100-101 -1.9429263457387442 1104 1 0.054256429442695264 1104 2 0.07955327057869965 1104 3 0.13243538195985138 1104 4 0.020483874462541962 1104 5 -0.014824125957183298 1104 6 0.005942872641050201 1104 7 0.047959883768854183 1104 8 -0.008295949196821084 1104 9 0.033921299264441984 1104 10-11 0.04696253391292515 1104 12-13 0.08399834125545169 1104 14-15 0.17473589827608293 1104 16-17 0.11602130476129702 1104 18-19 0.08434479471150524 1104 20-21 0.10594739068644543 1104 22-23 0.09689143102640685 1104 24-25 0.19810937177809507 1104 26-27 0.13532097194939752 1104 28-29 -0.03565458212530359 1104 30-31 0.013343772187070613 1104 32-33 -0.025354798169068715 1104 34-35 0.01876495937957401 1104 36-37 0.029492525571768624 1104 38-39 0.058030876131290654 1104 40-41 0.18770789328576143 1104 42-43 0.02516900284013701 1104 44-45 0.05348821385566538 1104 46-47 0.08557436836314736 1104 48-49 0.08862651112282549 1104 50-51 0.020500284867175367 1104 52-53 -0.05299183084121495 1104 54-55 -0.012125694267879794 1104 56-57 0.1541143206389961 1104 58-59 0.11354639210974682 1104 60-61 -0.10227360354799941 1104 62-63 -0.021168077605338453 1104 64-65 -0.03989981402361309 1104 66-67 -0.34531660053167457 1104 68-69 -0.20841242696335982 1104 70-71 -0.15357302785721672 1104 72-73 -0.46473199666793974 1104 74-75 -0.36107015392936503 1104 76-77 -0.3848225320615448 1104 78-79 -0.20908771988524677 1104 80-81 -0.549257763231882 1104 82-83 -0.5382765505055076 1104 84-85 -0.6767642319011564 1104 86-87 -0.7012144338534547 1104 88-89 -0.6503163843533812 1104 90-91 -0.7611334075933165 1104 92-93 -0.47631144475644405 1104 94-95 -0.5837872964524564 1104 96-97 -0.780475897704779 1104 98-99 -0.7855385129857844 1104 100-101 -1.357863746305945 1105 1 0.10811359231625062 1105 2 0.07413149138643149 1105 3 0.0571900140915389 1105 4 0.004645887313081687 1105 5 0.03339574767252884 1105 6 0.01919607359916853 1105 7 0.02842086177081171 1105 8 0.027592145707664884 1105 9 -0.028344091689469053 1105 10-11 0.07807387854885661 1105 12-13 0.07899507190791866 1105 14-15 0.09338385626830359 1105 16-17 0.05088422568979212 1105 18-19 0.020160330117199976 1105 20-21 0.052197148821456096 1105 22-23 0.1363788106777193 1105 24-25 0.016378185538705736 1105 26-27 0.11159318182816946 1105 28-29 0.016525038496943978 1105 30-31 0.11208657461839522 1105 32-33 0.20413488336978958 1105 34-35 0.23539943298126076 1105 36-37 0.1113116172269244 1105 38-39 0.07676874118062926 1105 40-41 0.034433708454201906 1105 42-43 0.1776795614982447 1105 44-45 0.1468425977838237 1105 46-47 0.07781050199272244 1105 48-49 0.24653633050805013 1105 50-51 0.13901296526510265 1105 52-53 0.13298416947905167 1105 54-55 0.14655342260796544 1105 56-57 0.18845314514175726 1105 58-59 0.14956859698440184 1105 60-61 -0.04373493517326921 1105 62-63 0.09301038206493217 1105 64-65 0.04170326716962336 1105 66-67 -0.016312150215746613 1105 68-69 0.01930897124984199 1105 70-71 -0.17712617477101844 1105 72-73 -0.069862703847825 1105 74-75 -0.0031750132473398196 1105 76-77 0.00894185473562814 1105 78-79 -0.052507490764035936 1105 80-81 -0.0283562906910646 1105 82-83 -0.13749340832892187 1105 84-85 -0.46186017724300044 1105 86-87 -0.7674188881726671 1105 88-89 -0.8639407229777163 1105 90-91 -1.1035883873433754 1105 92-93 -1.2427816169643258 1105 94-95 -1.2553409704651486 1105 96-97 -1.5369939091408256 1105 98-99 -1.624366185010178 1105 100-101 -2.1526124402349645 1106 1 0.2844135647452575 1106 2 0.26783611412331254 1106 3 0.23121078424134112 1106 4 0.14952595348346875 1106 5 0.12122407227504794 1106 6 0.19163978281707017 1106 7 0.2200117082003672 1106 8 0.1972989741241733 1106 9 0.2338193124094161 1106 10-11 0.18003233824925502 1106 12-13 0.13311785474038373 1106 14-15 0.20974080875429024 1106 16-17 0.3051751915936691 1106 18-19 0.3837574259723624 1106 20-21 0.40620689057312376 1106 22-23 0.3740849038676828 1106 24-25 0.37892942163832544 1106 26-27 0.31002898736075224 1106 28-29 0.38510880837103656 1106 30-31 0.3202429940651399 1106 32-33 0.3466603865404565 1106 34-35 0.3345566795578563 1106 36-37 0.2482898361406285 1106 38-39 0.40136206899976656 1106 40-41 0.3899920211092862 1106 42-43 0.27849566295950723 1106 44-45 0.1259695162655774 1106 46-47 0.07794008567069 1106 48-49 0.19295302015055427 1106 50-51 0.18988420871698963 1106 52-53 0.17125271097339834 1106 54-55 0.1378244454918871 1106 56-57 0.089971387950321 1106 58-59 0.19296166747425758 1106 60-61 0.014127393656799825 1106 62-63 0.1723247833148136 1106 64-65 0.04352203382710229 1106 66-67 -0.012607527478870395 1106 68-69 -0.18005781489113915 1106 70-71 -0.6982051197209316 1106 72-73 -0.5093278265387546 1106 74-75 -0.3207165994985992 1106 76-77 -0.38964651030434894 1106 78-79 -0.1624045590481984 1106 80-81 -0.11483455555642763 1106 82-83 -0.325588252552798 1106 84-85 -0.15499683747643545 1106 86-87 -0.6562544731372846 1106 88-89 -0.6908463382704291 1106 90-91 -0.7654595388919496 1106 92-93 -0.4195576485790653 1106 94-95 -0.5143622001315364 1106 96-97 -0.8468330783348321 1106 98-99 -0.9140831227916308 1106 100-101 -1.4554908521456351 1107 1 0.010687432538979635 1107 2 0.0036925147848450024 1107 3 0.011757049756447202 1107 4 -0.01768876060416602 1107 5 -0.08485100164620718 1107 6 -0.0605771864951663 1107 7 -0.02560897972666254 1107 8 -0.1587488700481643 1107 9 -0.1142269948691137 1107 10-11 -0.010863403552583861 1107 12-13 -0.06494448969088751 1107 14-15 -0.025797855818638027 1107 16-17 -0.06463553659330046 1107 18-19 -0.01675542880787617 1107 20-21 -0.08721086055030725 1107 22-23 -0.030834492734442165 1107 24-25 -0.05144962314876267 1107 26-27 -0.07301807165931962 1107 28-29 -0.0769375991397041 1107 30-31 -0.09695100536687562 1107 32-33 -0.10794645263696623 1107 34-35 -0.20699007041290685 1107 36-37 -0.21962182587638068 1107 38-39 -0.33505897308105403 1107 40-41 -0.49484167695790404 1107 42-43 -0.4754172352546959 1107 44-45 -0.47892649282213995 1107 46-47 -0.4472551165477867 1107 48-49 -0.5918678147837113 1107 50-51 -0.6969791914934973 1107 52-53 -0.6854169069748437 1107 54-55 -0.6342545374853472 1107 56-57 -0.7016762624397543 1107 58-59 -0.7409295137622749 1107 60-61 -0.5872391053993411 1107 62-63 -0.6109467958435744 1107 64-65 -0.796138011945601 1107 66-67 -0.7884082992668411 1107 68-69 -0.7419009180801943 1107 70-71 -0.7001135408966555 1107 72-73 -0.765232004551148 1107 74-75 -0.7105568961859277 1107 76-77 -0.7467163888563313 1107 78-79 -0.8972437698866429 1107 80-81 -0.8078923060116452 1107 82-83 -1.0630791063641283 1107 84-85 -0.9892258012424939 1107 86-87 -1.262797631454049 1107 88-89 -1.4018792311389063 1107 90-91 -1.59963504548222 1107 92-93 -1.5279504251781866 1107 94-95 -1.780682341793053 1107 96-97 -1.8298293530378942 1107 98-99 -1.8356094922141288 1107 100-101 -1.9799956215280385 1108 1 -0.0027263539824815552 1108 2 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-0.15931839043834373 2316 50-51 0.01718132566195152 2316 52-53 0.08598647198770237 2316 54-55 0.06376678439114158 2316 56-57 0.13261148379938703 2316 58-59 0.12550758251436633 2316 60-61 0.5116128722196791 2316 62-63 0.4250108321189714 2316 64-65 0.6714148709291834 2316 66-67 0.7041665144096001 2316 68-69 0.797802415328615 2316 70-71 0.9146362288390613 2316 72-73 1.1185054082156327 2316 74-75 1.2438463924482583 2316 76-77 1.143923109180914 2316 78-79 1.1229500976695768 2316 80-81 1.1014368853644498 2316 82-83 1.220592495737236 2316 84-85 1.227887430840287 2316 86-87 1.5199779911847209 2316 88-89 1.9146728694581618 2316 90-91 1.8858915993157304 2316 92-93 1.7213647470409974 2316 94-95 2.1803732291418605 2316 96-97 2.325469574197129 2316 98-99 2.7690260571831935 2316 100-101 3.07873181494568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 15.0 5 36.0 6 81.0 7 197.0 8 432.0 9 799.0 10 1473.0 11 2534.0 12 3870.0 13 6140.0 14 7869.0 15 11028.0 16 14844.0 17 21463.0 18 21947.0 19 15879.0 20 14385.0 21 15196.0 22 15972.0 23 17123.0 24 19465.0 25 22197.0 26 25431.0 27 30007.0 28 35886.0 29 43217.0 30 52137.0 31 63365.0 32 78753.0 33 102412.0 34 138791.0 35 199321.0 36 278429.0 37 342232.0 38 183688.0 39 9254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.078705928672825 18.687729611398805 14.911758498232164 22.32180596169621 2 15.831789774530892 20.250730382694915 39.71048665819426 24.206993184579932 3 19.65265738927243 24.260799881494087 30.072267444677287 26.0142752845562 4 12.92269879072606 16.88629083046611 36.50251048098666 33.68849989782117 5 13.510782008862595 37.49241311414797 33.835840393081426 15.160964483908007 6 31.870780857685542 36.20715289227896 16.790592443729455 15.131473806306042 7 28.492215206754594 33.44239820967296 19.870391725918257 18.194994857654187 8 26.967942610641177 34.194789943386866 19.06203835126426 19.7752290947077 9 26.552044604490405 16.696670644305026 20.085496023382497 36.66578872782207 10-11 25.43425954953385 26.475535853593215 26.311298181998392 21.778906414874548 12-13 26.630177093869744 23.992019402451923 27.13108042378688 22.24672307989145 14-15 22.9784066022486 25.253261227269412 26.224627242572275 25.54370492790971 16-17 21.858054492908572 28.555486877933877 26.956644003051515 22.629814626106036 18-19 21.844974561118743 26.585009073581485 29.918067703005725 21.65194866229405 20-21 22.586563749218346 25.479251595185183 30.19047003880564 21.74371461679083 22-23 22.28799029775491 25.486908038597385 30.289753228652582 21.935348434995124 24-25 22.234197552485032 25.76497719845764 30.258825835418495 21.741999413638826 26-27 21.68997149064549 26.020536314479507 30.36764202186404 21.92185017301096 28-29 21.572396687633702 26.569262909120976 30.159729428644376 21.69861097460094 30-31 21.776064162564765 25.958792491536563 29.97716633715027 22.287977008748403 32-33 21.567783467984654 26.113205109715445 30.037146279274758 22.281865143025147 34-35 22.409699073017034 26.13592446029945 29.75247227014487 21.701904196538653 36-37 22.447914216006318 25.6015573601387 29.96815730851996 21.982371115335024 38-39 21.88321519874064 26.160338962475908 29.834149389169262 22.12229644961419 40-41 22.22431656691306 26.017997690776163 29.849516229578164 21.908169512732613 42-43 21.952785475418114 26.097706095445293 29.412534465366747 22.536973963769846 44-45 22.010220658760574 26.20250045590653 29.53639526191308 22.250883623419814 46-47 22.56721111928418 26.577382228456276 28.828657514932864 22.02674913732668 48-49 22.33128779697098 26.656649190374647 28.64639060417521 22.365672408479163 50-51 22.193497767149264 26.952884747370042 28.696120416628034 22.157497068852656 52-53 22.628376617446257 26.891401389516922 28.315945809285576 22.164276183751248 54-55 22.088783256410714 26.868306395566403 28.44520154320438 22.597708804818502 56-57 22.368707940274668 27.015017098869926 28.210531767753878 22.40574319310153 58-59 22.473887632789673 26.62705382432316 28.256634854047274 22.642423688839887 60-61 22.058885356286 26.82597811630007 28.773092276110273 22.34204425130366 62-63 22.02443691150454 26.90920158850785 28.72352127223205 22.342840227755556 64-65 21.734992071850698 27.09232687701093 28.47233167789937 22.700349373239 66-67 21.82994819120303 27.050451621454403 28.20686317587981 22.91273701146276 68-69 22.030374604150673 26.768786115898948 28.492351206464928 22.708488073485455 70-71 22.19399568331661 26.891082966253737 27.974180096085327 22.94074125434432 72-73 22.072324916401463 26.905531564190593 28.151616145585358 22.87052737382259 74-75 21.898099043966727 27.53817464001517 27.76208351932074 22.801642796697365 76-77 22.034261736901918 27.003486212626388 28.053109395898584 22.90914265457311 78-79 22.189831108873314 27.202176430322318 27.95871376955883 22.649278691245545 80-81 22.331185198138183 27.313876856831946 27.718489964169287 22.636447980860584 82-83 22.25710078642482 27.22630747965564 27.914363052153806 22.602228681765734 84-85 22.146848288143783 27.39891701023535 27.70640924426028 22.747825457360584 86-87 22.297782225105 27.303977445998257 27.606622828992126 22.791617499904614 88-89 22.10263844851007 27.41156020622817 27.29707873128572 23.18872261397604 90-91 22.133596453558223 27.292819279739017 27.465011526826956 23.108572739875804 92-93 22.272636766043004 27.113351963786187 27.559953716274077 23.05405755389673 94-95 22.033741614648786 26.914627928625073 27.72412706165264 23.327503395073503 96-97 22.06714325533656 26.704540549902724 27.813607476239948 23.414708718520767 98-99 21.96740948812592 26.987246028793617 27.248150557227476 23.797193925852994 100-101 22.323291668638987 26.199138490325417 27.40007295266152 24.077496888374075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1430.0 1 951.5 2 724.0 3 961.0 4 749.0 5 672.0 6 1304.0 7 2862.5 8 4575.0 9 5171.0 10 4154.5 11 2890.0 12 2582.5 13 2722.5 14 2950.5 15 3081.5 16 3201.0 17 3351.5 18 3520.0 19 3820.0 20 4165.5 21 4710.5 22 5387.5 23 6299.5 24 7640.0 25 9319.5 26 11487.0 27 13999.0 28 16121.5 29 18203.5 30 20420.0 31 23663.5 32 28378.5 33 31075.0 34 32120.0 35 33942.5 36 37310.0 37 40804.0 38 43312.5 39 45073.5 40 46758.0 41 49828.5 42 52178.5 43 54112.5 44 56303.0 45 59219.5 46 60880.5 47 64069.0 48 70638.5 49 70550.5 50 66280.0 51 63749.0 52 62167.0 53 62595.0 54 63203.0 55 59909.5 56 54544.0 57 49798.5 58 45785.0 59 40716.5 60 33635.5 61 28242.0 62 23932.0 63 18054.0 64 13079.0 65 10225.0 66 7851.0 67 6243.0 68 4805.5 69 3436.0 70 2486.5 71 1686.0 72 1232.5 73 1023.5 74 790.0 75 579.5 76 378.5 77 212.5 78 130.5 79 78.5 80 42.0 81 24.5 82 11.5 83 5.5 84 5.0 85 3.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009800247567617531 2 0.01330829073102608 3 0.010134346916513584 4 1.113664496320174E-4 5 6.125154729760957E-4 6 0.002227328992640348 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.744564342801523E-4 14-15 0.0 16-17 0.002394378667088374 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002867686078024448 26-27 0.017929998390754803 28-29 0.006542778915881022 30-31 0.003313151876552518 32-33 0.0 34-35 0.00863089984648135 36-37 7.517235350161175E-4 38-39 0.002227328992640348 40-41 0.0017540215817042743 42-43 0.002700636403576422 44-45 2.784161240800435E-5 46-47 1.113664496320174E-4 48-49 6.403570853841001E-4 50-51 0.004148400248792648 52-53 0.007851334699057227 54-55 0.007183136001265122 56-57 0.015953243909786495 58-59 0.02291364701178758 60-61 0.003424518326184535 62-63 0.019572653522827062 64-65 0.020714159631555238 66-67 0.01291850815731402 68-69 0.02920585141599657 70-71 0.01567482778570645 72-73 0.0049001237838087654 74-75 0.015396411661626407 76-77 0.020686318019147235 78-79 0.025391550516099967 80-81 0.011526427536913802 82-83 0.03321504360274919 84-85 0.02118746704249131 86-87 0.029929733338604676 88-89 0.02149372477897936 90-91 0.027618879508740316 92-93 0.023693212159211705 94-95 0.014282747165306232 96-97 0.014728212963834302 98-99 0.010969595288753714 100-101 0.010468446265409636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1795873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.90727475977152 #Duplication Level Percentage of deduplicated Percentage of total 1 78.02537915018469 43.62186310384723 2 12.686802776953499 14.185691373483431 3 3.9309227550242007 6.593015355737281 4 1.7469593572419568 3.9067094711791968 5 0.9028642706087591 2.52383404238523 6 0.5729517642450083 1.9219303024644907 7 0.3880426305738171 1.5186084176196435 8 0.283649552603094 1.2686458778293952 9 0.2019365770308442 1.0160751326500055 >10 1.1413797412630324 11.814827514640827 >50 0.0768785364545732 2.9130661736903836 >100 0.03585891873651799 3.754043327128885 >500 0.0041829172087536354 1.702579412045277 >1k 0.0018922720706266445 1.7034702901469423 >5k 9.959326687508654E-5 0.35785459746271814 >10k+ 1.991865337501731E-4 1.197785607689123 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11480 0.63924342088778 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10032 0.5586141113541992 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6427 0.3578760858924879 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3665 0.2040790189506719 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.14550026644423075 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2073 0.11543132504358605 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1845 0.10273554978553606 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1840 0.10245713366145602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.568322481600871E-5 0.0 10-11 0.0 0.0 0.0 8.352483722401307E-5 0.0 12-13 0.0 0.0 0.0 1.1136644963201741E-4 0.0 14-15 0.0 0.0 0.0 1.1136644963201741E-4 0.0 16-17 0.0 0.0 0.0 1.670496744480261E-4 0.0 18-19 0.0 0.0 0.0 1.670496744480261E-4 0.0 20-21 0.0 0.0 0.0 1.670496744480261E-4 5.568322481600871E-5 22-23 0.0 0.0 0.0 5.011490233440784E-4 5.568322481600871E-5 24-25 0.0 0.0 0.0 9.46614821872148E-4 5.568322481600871E-5 26-27 0.0 0.0 0.0 0.0018375464189282873 5.568322481600871E-5 28-29 0.0 0.0 0.0 0.0052620647451128225 8.352483722401307E-5 30-31 0.0 0.0 0.0 0.015145837149954367 1.1136644963201741E-4 32-33 0.0 0.0 0.0 0.03638898741726169 1.1136644963201741E-4 34-35 0.0 0.0 0.0 0.06810058394997864 1.1136644963201741E-4 36-37 0.0 0.0 0.0 0.11738023791214636 1.1136644963201741E-4 38-39 0.0 0.0 0.0 0.1913275604678059 1.3920806204002176E-4 40-41 0.0 0.0 0.0 0.2693397584350341 1.670496744480261E-4 42-43 0.0 0.0 0.0 0.3499690679686147 1.670496744480261E-4 44-45 0.0 0.0 0.0 0.44677435431124585 1.9489128685603045E-4 46-47 0.0 0.0 0.0 0.557082822671759 2.2273289926403483E-4 48-49 0.0 0.0 0.0 0.6692009958387926 2.2273289926403483E-4 50-51 0.0 0.0 0.0 0.7941263107135081 2.2273289926403483E-4 52-53 0.0 0.0 0.0 0.9113394989512065 2.784161240800435E-4 54-55 0.0 0.0 0.0 1.036765962849266 2.784161240800435E-4 56-57 0.0 0.0 0.0 1.1825168038051688 2.784161240800435E-4 58-59 0.0 0.0 0.0 1.3387360910264814 3.340993488960522E-4 60-61 0.0 0.0 0.0 1.5042266351796592 3.340993488960522E-4 62-63 0.0 0.0 0.0 1.6667659684175886 3.340993488960522E-4 64-65 0.0 0.0 0.0 1.8282473203840137 3.340993488960522E-4 66-67 0.0 0.0 0.0 2.0171805021847313 3.897825737120609E-4 68-69 0.0 0.0 0.0 2.2232084340039635 3.897825737120609E-4 70-71 0.0 0.0 0.0 2.438257048243389 4.176241861200653E-4 72-73 0.0 0.0 0.0 2.6617416710424404 4.4546579852806965E-4 74-75 0.0 0.0 0.0 2.871723111823609 4.4546579852806965E-4 76-77 0.0 0.0 0.0 3.0962100326693482 4.73307410936074E-4 78-79 0.0 0.0 0.0 3.3545801958156285 5.289906357520827E-4 80-81 0.0 0.0 0.0 3.627956988049823 5.56832248160087E-4 82-83 0.0 0.0 0.0 3.9056213885948505 5.56832248160087E-4 84-85 0.0 0.0 0.0 4.182172124643558 5.56832248160087E-4 86-87 0.0 0.0 0.0 4.466518512166506 6.125154729760957E-4 88-89 0.0 0.0 0.0 4.772247258018802 6.681986977921044E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9760 0.0 29.695883 1 GTATCAA 13340 0.0 26.784172 1 TAAGGTG 950 0.0 25.993586 5 GTAAGGT 930 0.0 24.51008 4 GGTAAGG 940 0.0 23.748106 3 TATACCG 100 0.0016563842 23.744139 5 AGGTAAG 920 0.0 22.203026 2 GTACATG 8535 0.0 22.044888 1 GAGTACT 9135 0.0 21.991484 12-13 TATCAAC 16100 0.0 21.952227 2 ATCAACG 16180 0.0 21.72262 3 TCAACGC 16260 0.0 21.641336 4 CCGTCGT 110 0.0028811824 21.586784 9 CAACGCA 16305 0.0 21.58161 5 TACATGG 8550 0.0 21.501877 2 GTATAGG 535 0.0 21.314428 1 AACGCAG 16680 0.0 21.039473 6 ACATGGG 8250 0.0 20.783192 3 GTACTTT 9645 0.0 20.75362 14-15 AGAGTAC 14125 0.0 20.358624 10-11 >>END_MODULE