FastQCFastQC Report
Thu 26 May 2016
SRR938031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979574
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274141.384843405702439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206451.0429011494392226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144480.7298539988906705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68660.3468423004141295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47950.24222383199617695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.21393491731049205No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33290.1681674946225804No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.14361675794893244No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26450.13361460597077957No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.12982591203966107No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23050.1164391934830423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21100.10658858926213417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112350.042.9636271
GTATCAA183900.036.2333261
TATCAAC224650.029.4931342
ATCAACG227550.029.0754833
TCAACGC229000.028.8291154
CAACGCA232000.028.4358395
AACGCAG237150.027.8133986
GTACATG151850.027.0132711
TACATGG155200.025.8473072
GAGTACT146900.025.14083712-13
ACGCAGA262700.025.0464237
CGCAGAG264450.024.8088198
ACATGGG157150.024.6797263
GCAGAGT271400.023.7866159
GTACTTT157350.023.42796114-15
AGTACTT149650.022.67914212-13
CATGGGA102750.022.434924
ATGGGAG34100.021.3256175
CATGGGG50150.021.2295914
ACTTTTT175900.021.18445216-17
Produced by FastQC (version 0.11.5)