FastQCFastQC Report
Thu 26 May 2016
SRR938031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979574
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262481.3259418440533166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205551.0383547167218807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147690.7460696089158576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67110.33901233295648453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.25641880525810096No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44780.22621028564731605No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.15730657202004067No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.13250325575098482No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25880.1307351985831295No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.12649186138027677No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.11835879840814235No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20890.10552775496142099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117450.040.08931
GTATCAA189200.034.840821
TATCAAC231850.028.4111612
ATCAACG232650.028.1851043
TCAACGC234450.027.9889934
CAACGCA237500.027.6295595
AACGCAG242550.027.0816066
CATTCCG3200.026.7162869
CGGCGAT458.954929E-426.38712382-83
GTACATG151950.025.3843731
TACATGG155100.024.5621872
GAGTACT152750.024.42409712-13
ACGCAGA270300.024.1783087
CGCAGAG271150.024.0858
ATAGCGT1201.7124951E-423.747816
ACATGGG156150.023.5089763
GCAGAGT278200.023.2868549
GTACTTT160850.023.03175714-15
CATGGGG49850.022.6069324
AGTACTT153800.022.4662312-13
Produced by FastQC (version 0.11.5)