##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938031_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1979574 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50824874442683 33.0 31.0 34.0 31.0 34.0 2 32.71281093811093 34.0 31.0 34.0 31.0 34.0 3 32.76146332493759 34.0 31.0 34.0 31.0 34.0 4 36.22736154344319 37.0 35.0 37.0 35.0 37.0 5 36.168163958508245 37.0 35.0 37.0 35.0 37.0 6 36.23858314970797 37.0 37.0 37.0 35.0 37.0 7 36.211038334510356 37.0 37.0 37.0 35.0 37.0 8 36.221545140520135 37.0 37.0 37.0 35.0 37.0 9 37.894402027911056 39.0 38.0 39.0 35.0 39.0 10-11 37.87792373510665 39.0 38.0 39.0 35.0 39.0 12-13 37.796713838431906 39.0 38.0 39.0 35.0 39.0 14-15 38.99198413396014 40.0 38.0 41.0 35.5 41.0 16-17 38.91920256580457 40.0 38.0 41.0 35.5 41.0 18-19 39.21233128945924 40.5 39.0 41.0 36.0 41.0 20-21 39.2163157325768 41.0 39.0 41.0 36.0 41.0 22-23 39.09937744181324 40.0 39.0 41.0 35.0 41.0 24-25 38.98599168305908 40.0 38.0 41.0 35.0 41.0 26-27 38.811297026531975 40.0 38.0 41.0 35.0 41.0 28-29 38.6740460321261 40.0 38.0 41.0 35.0 41.0 30-31 38.37380163610959 40.0 37.5 41.0 34.0 41.0 32-33 38.08426863557513 40.0 37.0 41.0 34.0 41.0 34-35 37.929978874242636 40.0 37.0 41.0 34.0 41.0 36-37 37.84027043192121 40.0 37.0 41.0 33.0 41.0 38-39 37.64344525640365 40.0 36.0 41.0 33.0 41.0 40-41 37.40630711456102 40.0 36.0 41.0 33.0 41.0 42-43 37.11733888200189 40.0 35.5 41.0 32.5 41.0 44-45 36.77034654930809 39.5 35.0 41.0 31.5 41.0 46-47 36.26210992870183 39.0 35.0 41.0 30.5 41.0 48-49 35.754851296289004 39.0 35.0 41.0 28.5 41.0 50-51 35.37305046439285 39.0 35.0 40.0 27.0 41.0 52-53 34.88023483840463 38.0 35.0 40.0 26.0 41.0 54-55 34.54388848307767 38.0 34.0 40.0 25.0 41.0 56-57 34.20720316593368 38.0 34.0 40.0 24.0 41.0 58-59 33.75761098094843 37.0 33.0 40.0 22.5 41.0 60-61 33.33362076891291 37.0 33.0 40.0 21.5 41.0 62-63 32.8294408291885 36.0 32.5 39.0 19.5 40.5 64-65 32.315780819509655 35.5 31.5 39.0 17.0 40.0 66-67 31.80073111689687 35.0 31.0 38.5 12.0 40.0 68-69 31.241114502413147 35.0 30.5 38.0 7.5 40.0 70-71 30.673111740202692 35.0 30.0 37.0 6.0 39.5 72-73 30.052842177155284 34.0 29.0 36.5 2.0 39.0 74-75 29.438572389817203 34.0 28.5 36.0 2.0 39.0 76-77 28.08404889132712 32.5 27.0 35.0 2.0 36.5 78-79 28.767675014927455 34.0 28.5 35.0 2.0 37.0 80-81 28.508933992869174 34.0 28.0 35.0 2.0 37.0 82-83 27.989636659200414 33.5 27.0 35.0 2.0 36.5 84-85 27.376452964122585 33.0 26.0 35.0 2.0 36.0 86-87 26.696743087149052 33.0 25.0 35.0 2.0 35.5 88-89 25.935236318521056 32.0 23.5 35.0 2.0 35.0 90-91 25.318043932684507 32.0 20.0 34.0 2.0 35.0 92-93 24.43927759204758 31.0 14.5 34.0 2.0 35.0 94-95 23.588354868269636 31.0 4.5 34.0 2.0 35.0 96-97 22.578578775029374 30.5 2.0 34.0 2.0 35.0 98-99 21.238229033115203 29.5 2.0 34.0 2.0 35.0 100-101 17.3360851880253 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23168575611311581 1101 2 0.21771501656398584 1101 3 0.17832214809101288 1101 4 0.07949077619511513 1101 5 0.00396127827054471 1101 6 0.07610125113214394 1101 7 0.12942083307939356 1101 8 0.04840293929628103 1101 9 0.1360969594276611 1101 10-11 0.2131005278971756 1101 12-13 0.2555090506379045 1101 14-15 0.3866857370999881 1101 16-17 0.4040594066678409 1101 18-19 0.32089141453531767 1101 20-21 0.23354715573419327 1101 22-23 0.2961994181953216 1101 24-25 0.3309943973309828 1101 26-27 0.38696506633038297 1101 28-29 0.4940288967423285 1101 30-31 0.5595601975147844 1101 32-33 0.5415775610619491 1101 34-35 0.46919987050543455 1101 36-37 0.46766662310009366 1101 38-39 0.513351010903115 1101 40-41 0.6436622556839211 1101 42-43 0.5530845507637778 1101 44-45 0.599291197957271 1101 46-47 0.6761159370003895 1101 48-49 0.7736670711337368 1101 50-51 0.7745631656075176 1101 52-53 1.0107233575686507 1101 54-55 0.9751694977613496 1101 56-57 0.9911722973499764 1101 58-59 1.234320715255329 1101 60-61 1.0667936925211308 1101 62-63 1.1753230769160155 1101 64-65 1.2831683688112179 1101 66-67 1.3006730916284006 1101 68-69 1.5013513018517344 1101 70-71 1.705505019569042 1101 72-73 1.6587464749916272 1101 74-75 1.7505186405453905 1101 76-77 1.700466951110883 1101 78-79 1.9540959070696609 1101 80-81 1.9890628256364344 1101 82-83 2.118224629570637 1101 84-85 2.364880478750571 1101 86-87 2.4473683644442517 1101 88-89 2.9191088858897487 1101 90-91 3.2858822160954126 1101 92-93 3.3584764812757015 1101 94-95 3.683622832617896 1101 96-97 3.6863240009315916 1101 98-99 3.911268071380391 1101 100-101 3.9041824161520005 1102 1 0.15134776379690607 1102 2 0.1141135363744894 1102 3 0.17313971709188536 1102 4 0.1223496867945002 1102 5 -0.079602843632955 1102 6 -0.004967407976444349 1102 7 0.08715411613028579 1102 8 0.08345726790777519 1102 9 0.11144579389628007 1102 10-11 0.23029918677006123 1102 12-13 0.24819059776591246 1102 14-15 0.3796191874971342 1102 16-17 0.3356094809130141 1102 18-19 0.34745468792254997 1102 20-21 0.30752864158046833 1102 22-23 0.36405862341840844 1102 24-25 0.42649077935186597 1102 26-27 0.3680251813514772 1102 28-29 0.27010751413219936 1102 30-31 0.37399292903008785 1102 32-33 0.23935550906676184 1102 34-35 0.3029510784626268 1102 36-37 0.36784663818482244 1102 38-39 0.4767292841724995 1102 40-41 0.5437803997902719 1102 42-43 0.5696807041566601 1102 44-45 0.4991088258828853 1102 46-47 0.39803682464269485 1102 48-49 0.4150083750204132 1102 50-51 0.49443052601480275 1102 52-53 0.6387844978802377 1102 54-55 0.7520052980771865 1102 56-57 0.5876762924474406 1102 58-59 0.6949648805274435 1102 60-61 0.5842339428334284 1102 62-63 0.6969631881659311 1102 64-65 0.6917127509263779 1102 66-67 0.9052394644570221 1102 68-69 0.9802901261852952 1102 70-71 1.1412137074329003 1102 72-73 1.4048250334567562 1102 74-75 1.3139702751177147 1102 76-77 1.413410654413024 1102 78-79 1.5890438765230748 1102 80-81 1.7869318123665785 1102 82-83 1.71169989296423 1102 84-85 1.8127895343048124 1102 86-87 2.0230439366034894 1102 88-89 2.082629462409418 1102 90-91 2.2177858387885045 1102 92-93 2.520535724210628 1102 94-95 2.7132001065236615 1102 96-97 3.0135036271130353 1102 98-99 3.5317072964021925 1102 100-101 3.5321021370844257 1103 1 0.17154808795680765 1103 2 0.12884504982379497 1103 3 0.17302315274281455 1103 4 0.1462204002793257 1103 5 0.05780987187232256 1103 6 0.04803329140965218 1103 7 0.1359584989498117 1103 8 0.12445045166870017 1103 9 0.21520471661966667 1103 10-11 0.16647465497020164 1103 12-13 0.25507830189228997 1103 14-15 0.3900267186642239 1103 16-17 0.42420601839678085 1103 18-19 0.26730212739234815 1103 20-21 0.26109578819622214 1103 22-23 0.23312008613446977 1103 24-25 0.3037200725278595 1103 26-27 0.34016560808800733 1103 28-29 0.33710634293802144 1103 30-31 0.3371988371773895 1103 32-33 0.45472132684892586 1103 34-35 0.4566467233965241 1103 36-37 0.4696496388842064 1103 38-39 0.5448771044191787 1103 40-41 0.63507942620042 1103 42-43 0.6553041569036964 1103 44-45 0.8280652141642832 1103 46-47 1.0445804141585668 1103 48-49 1.0885629770919323 1103 50-51 1.1156283079617566 1103 52-53 1.232298940758156 1103 54-55 1.4296472845557204 1103 56-57 1.4916009030670097 1103 58-59 1.6935688836802356 1103 60-61 1.7683841054684493 1103 62-63 1.7160499064909587 1103 64-65 1.8073203009657846 1103 66-67 1.7488253723822886 1103 68-69 2.1353005892232915 1103 70-71 2.1604607017379003 1103 72-73 2.1555119305137858 1103 74-75 2.2492028209941104 1103 76-77 2.253712639337369 1103 78-79 2.4757183511509027 1103 80-81 2.4823644326221306 1103 82-83 2.698283891454448 1103 84-85 2.8066872518638455 1103 86-87 2.8274794876198186 1103 88-89 3.0625089293217975 1103 90-91 3.330670084839195 1103 92-93 3.874165593547545 1103 94-95 3.617561833452246 1103 96-97 3.873007507040654 1103 98-99 4.3563743941719295 1103 100-101 4.150414257556456 1104 1 0.23922212140810473 1104 2 0.2643734454063278 1104 3 0.2499753246292471 1104 4 0.1252293607268058 1104 5 0.060807103794530803 1104 6 0.09788863786205582 1104 7 0.13566554622066462 1104 8 0.2212016710030582 1104 9 0.23245116928762144 1104 10-11 0.3115207592197109 1104 12-13 0.3059280601086414 1104 14-15 0.4211529461361678 1104 16-17 0.4485923800801217 1104 18-19 0.3248123286573801 1104 20-21 0.39709388465448825 1104 22-23 0.40677009485794713 1104 24-25 0.40184003211339814 1104 26-27 0.43131524573804114 1104 28-29 0.45234287948159846 1104 30-31 0.44415360006795623 1104 32-33 0.5401300675167491 1104 34-35 0.44710460200211344 1104 36-37 0.5410737927716198 1104 38-39 0.6277755657012349 1104 40-41 0.7407279454220301 1104 42-43 0.821349438281537 1104 44-45 0.8641296481405476 1104 46-47 1.0207330854968504 1104 48-49 1.176327708708179 1104 50-51 1.0850368819720302 1104 52-53 1.1054282748310413 1104 54-55 1.1439916823881262 1104 56-57 1.1798184745019 1104 58-59 1.3558500479915594 1104 60-61 1.3792270020808743 1104 62-63 1.519608500942816 1104 64-65 1.5970785433535752 1104 66-67 1.786397536404575 1104 68-69 1.817769451603219 1104 70-71 1.916325940878469 1104 72-73 2.058835902379908 1104 74-75 2.1306790293264655 1104 76-77 1.8641128518257482 1104 78-79 1.9838028699662793 1104 80-81 2.1650920699128022 1104 82-83 2.245000737668583 1104 84-85 2.409912075506302 1104 86-87 2.5545357553760084 1104 88-89 2.652077598743521 1104 90-91 2.835786015999208 1104 92-93 3.1708030170217825 1104 94-95 3.2536706009415255 1104 96-97 3.7367005082046525 1104 98-99 4.3871513568901115 1104 100-101 4.001396690808804 1105 1 0.1752950798976869 1105 2 0.18319657126426137 1105 3 0.24282312711802234 1105 4 0.07756941886237456 1105 5 -0.0010897334804695902 1105 6 0.006993660508179289 1105 7 0.14243637092478423 1105 8 0.10352438173838152 1105 9 0.13638817619528254 1105 10-11 0.0650632615775919 1105 12-13 0.18698944855110966 1105 14-15 0.3265477912521675 1105 16-17 0.26860405137664856 1105 18-19 0.21753151370388224 1105 20-21 0.1688190949617976 1105 22-23 0.26772212165652576 1105 24-25 0.24584244614497663 1105 26-27 0.20712709731989065 1105 28-29 0.2695916689374229 1105 30-31 0.336504145188691 1105 32-33 0.5182083412939491 1105 34-35 0.6130405853702143 1105 36-37 0.5443387796797694 1105 38-39 0.6231621206376374 1105 40-41 0.5119189883462596 1105 42-43 0.5820564108073469 1105 44-45 0.7424476375848386 1105 46-47 0.8120520524582417 1105 48-49 0.9592494171356165 1105 50-51 1.1198706703141141 1105 52-53 1.299852622800131 1105 54-55 1.3881084775801966 1105 56-57 1.2789059104309644 1105 58-59 1.4441620913336735 1105 60-61 1.5000144893710043 1105 62-63 1.4687822136485167 1105 64-65 1.564801916444182 1105 66-67 1.672753348949552 1105 68-69 1.735866649817055 1105 70-71 1.7610568904936876 1105 72-73 1.810547170949203 1105 74-75 1.7088426594310455 1105 76-77 1.8437041907664558 1105 78-79 1.9755929228552915 1105 80-81 1.9561221692170072 1105 82-83 1.981180038728514 1105 84-85 2.0160196244788082 1105 86-87 2.2845409896640945 1105 88-89 2.337618690973148 1105 90-91 2.5041513717620525 1105 92-93 2.8756298222221943 1105 94-95 3.0409777898342796 1105 96-97 3.4830035237804964 1105 98-99 3.7164182988324654 1105 100-101 3.7327404117187726 1106 1 0.16026402288363784 1106 2 0.10848943935486233 1106 3 0.14447143444353117 1106 4 0.13008400624776328 1106 5 0.09042314195983892 1106 6 0.09065489257084636 1106 7 0.1464876754986335 1106 8 0.15021297803304634 1106 9 0.23692685629459476 1106 10-11 0.19623769175084504 1106 12-13 0.13229235527251149 1106 14-15 0.15006955805119304 1106 16-17 0.15683913459869103 1106 18-19 0.0446429873211045 1106 20-21 0.03823174542922203 1106 22-23 0.03101210364076934 1106 24-25 0.14625833227822227 1106 26-27 0.0402931201398502 1106 28-29 0.09133874921958096 1106 30-31 0.21683828818540007 1106 32-33 0.23158750575719722 1106 34-35 0.11814056834039377 1106 36-37 0.25314678190260764 1106 38-39 0.21309301542023462 1106 40-41 0.2531374271599063 1106 42-43 0.03115965633254092 1106 44-45 0.10235047797957009 1106 46-47 0.30865505149919414 1106 48-49 0.5012324052416375 1106 50-51 0.4150153789697484 1106 52-53 0.6026562689742434 1106 54-55 0.5573311111074304 1106 56-57 0.564003025220913 1106 58-59 0.8091335439773317 1106 60-61 0.6930743895224865 1106 62-63 0.6678608107498647 1106 64-65 0.7249046331486397 1106 66-67 0.9960331130417295 1106 68-69 1.0416549960422614 1106 70-71 0.8635047030581084 1106 72-73 1.2601299403564745 1106 74-75 1.3356789670075244 1106 76-77 1.3839752337981253 1106 78-79 1.4194527763988383 1106 80-81 1.2081859145225984 1106 82-83 1.5411115609255397 1106 84-85 1.639006296403803 1106 86-87 1.933937327356464 1106 88-89 2.0583430857423046 1106 90-91 1.9960129103614825 1106 92-93 2.0628726943461757 1106 94-95 2.28410984264465 1106 96-97 2.6817280798847882 1106 98-99 3.03945157868619 1106 100-101 3.1497693355237715 1107 1 0.2238575047776621 1107 2 0.1980651443933823 1107 3 0.20534299571421855 1107 4 0.09693481186263142 1107 5 0.04224774082588567 1107 6 0.09714633437713616 1107 7 0.12073778762201925 1107 8 0.20933547031829391 1107 9 0.15004127569628878 1107 10-11 0.23610592167407418 1107 12-13 0.24847323762217144 1107 14-15 0.41677371074426617 1107 16-17 0.3098047143937279 1107 18-19 0.27149522655336256 1107 20-21 0.2123357903499823 1107 22-23 0.38451342122996124 1107 24-25 0.38609094489807205 1107 26-27 0.3974525328141425 1107 28-29 0.43658491303798286 1107 30-31 0.5253541427663961 1107 32-33 0.5299988680395629 1107 34-35 0.4591865679342959 1107 36-37 0.5023390472858225 1107 38-39 0.5354262639637497 1107 40-41 0.6229094652617775 1107 42-43 0.6667851597721111 1107 44-45 0.6843678955939936 1107 46-47 0.6108696835448413 1107 48-49 0.7232952746630801 1107 50-51 0.8545578653643275 1107 52-53 0.8763193231482447 1107 54-55 0.9375078923072948 1107 56-57 1.0855867763672506 1107 58-59 1.080197957466055 1107 60-61 1.2316533400029925 1107 62-63 1.2938354753280166 1107 64-65 1.3734400842193395 1107 66-67 1.3519555426271346 1107 68-69 1.6156897587534829 1107 70-71 1.785248997037808 1107 72-73 1.758436514691489 1107 74-75 1.720376105574175 1107 76-77 1.7576420254182104 1107 78-79 1.6625630455618996 1107 80-81 1.6363251461036832 1107 82-83 2.0079821694263877 1107 84-85 2.185268487729804 1107 86-87 2.312963030944637 1107 88-89 2.3040260565991524 1107 90-91 2.5989849888500807 1107 92-93 2.681942008909715 1107 94-95 2.77112361206542 1107 96-97 2.9601906842264754 1107 98-99 3.027453818922531 1107 100-101 3.230305859855104 1108 1 0.20248168825657586 1108 2 0.20816883999019353 1108 3 0.13506969776059918 1108 4 0.03777790214973464 1108 5 -0.003681355201813119 1108 6 0.09968107739239684 1108 7 0.05782350426768801 1108 8 0.08213198190617987 1108 9 0.1872502018463038 1108 10-11 0.12052387722228275 1108 12-13 0.1951520015348649 1108 14-15 0.2404017144061399 1108 16-17 0.24056045671378 1108 18-19 0.2580398366537864 1108 20-21 0.2538635021636395 1108 22-23 0.22455186397196059 1108 24-25 0.23303626121609966 1108 26-27 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1.3542514574265496 2315 84-85 1.550630857529434 2315 86-87 1.8929317930474099 2315 88-89 2.050915006638899 2315 90-91 2.3225741875699875 2315 92-93 2.5729342325587936 2315 94-95 2.851277100721937 2315 96-97 3.342742815333917 2315 98-99 3.567920399977993 2315 100-101 3.8150647725928266 2316 1 -0.3434501230288447 2316 2 -0.4604922402436742 2316 3 -0.415321879883642 2316 4 -0.30368350936253563 2316 5 -0.3040800650142046 2316 6 -0.3459620755964963 2316 7 -0.2619874543735179 2316 8 -0.3144012224429744 2316 9 -0.38584047351382367 2316 10-11 -0.3811587741461011 2316 12-13 -0.47725887013711343 2316 14-15 -0.42231351497735403 2316 16-17 -0.339248937172961 2316 18-19 -0.5298877368269999 2316 20-21 -0.5004571869161865 2316 22-23 -0.4786014620822314 2316 24-25 -0.5513453279346976 2316 26-27 -0.5686970214310918 2316 28-29 -0.4750997146276603 2316 30-31 -0.4197843128895613 2316 32-33 -0.44905921088821543 2316 34-35 -0.35109555894314326 2316 36-37 -0.382359221347663 2316 38-39 -0.4266208538912579 2316 40-41 -0.33014038279924307 2316 42-43 -0.24894991633154717 2316 44-45 -0.2722012014255881 2316 46-47 -0.4521288138356354 2316 48-49 -0.044117335266285806 2316 50-51 -0.13901114109559387 2316 52-53 -0.1965711399134733 2316 54-55 0.07398339424258182 2316 56-57 0.055397707924271344 2316 58-59 0.2291518257051095 2316 60-61 0.4475303767567951 2316 62-63 0.4448860512203723 2316 64-65 0.5682678699350561 2316 66-67 0.5612619730337194 2316 68-69 0.7152395598576433 2316 70-71 0.8724067989201103 2316 72-73 0.8742627374145862 2316 74-75 1.2272615744384225 2316 76-77 1.4122063999192953 2316 78-79 1.410761914572383 2316 80-81 1.4883917510556195 2316 82-83 1.7087722185969305 2316 84-85 1.9222492790199794 2316 86-87 1.987630391902016 2316 88-89 2.2291952689984456 2316 90-91 2.306578332379761 2316 92-93 2.3364925479073904 2316 94-95 2.861122303981464 2316 96-97 3.123952733692171 2316 98-99 3.2829058457818547 2316 100-101 3.7034880424099583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 14.0 5 47.0 6 125.0 7 259.0 8 584.0 9 1093.0 10 1837.0 11 3103.0 12 4850.0 13 7723.0 14 9451.0 15 13095.0 16 18455.0 17 27711.0 18 28358.0 19 18703.0 20 15693.0 21 16776.0 22 17724.0 23 19766.0 24 22473.0 25 25525.0 26 29607.0 27 34418.0 28 41259.0 29 50276.0 30 61129.0 31 73504.0 32 90415.0 33 114544.0 34 154653.0 35 227506.0 36 282112.0 37 356080.0 38 202113.0 39 8588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.546250860033524 19.541780201194804 15.000803203113398 20.911165735658276 2 15.56964493944226 21.242499752433726 40.39584331176981 22.79201199635421 3 18.699293365475743 27.046805191551098 30.66050222174624 23.59339922122692 4 12.164916685399927 17.873804333824186 37.1439901593548 32.81728882142109 5 12.545082943512229 39.058481510872255 33.34795004422946 15.048485501386057 6 29.643817126218636 37.21787476390111 17.59692620580297 15.541381904077292 7 27.160591117078724 35.58866705664956 20.193789168780757 17.056952657490957 8 26.77452499833297 34.36389280107013 19.459964073042052 19.401618127554844 9 27.802799996362854 16.60119803553694 20.336850251619794 35.25915171648041 10-11 24.95880426798897 27.59548266445205 26.130975654357957 21.31473741320102 12-13 26.281513093221065 25.650720811649375 27.436509067102318 20.631257028027242 14-15 23.153289546134673 26.175606468866537 27.225125203705442 23.44597878129335 16-17 20.62415954139628 29.94927191405828 27.66777094465779 21.758797599887654 18-19 20.472384462515674 27.085473945404416 32.02977004143316 20.412371550646753 20-21 21.703204830938375 26.069396748997516 32.1744476336828 20.052950786381313 22-23 21.173761143845525 26.331426856768747 31.972203026114787 20.522608973270938 24-25 20.76815283342266 26.75100160792739 32.30168516650359 20.179160392146365 26-27 20.529522802034798 26.90640863689443 32.33134365078583 20.232724910284944 28-29 20.095283126571676 26.91192650539965 32.75078375448455 20.242006613544124 30-31 20.278226527525618 26.859137369959395 32.55599437050598 20.30664173200901 32-33 20.171865259899352 27.038039497386812 32.224635199290354 20.565460043423485 34-35 20.720778751655196 27.015827739390204 31.995409657502655 20.267983851451948 36-37 21.027075686439726 26.69777455821035 31.849065601380506 20.426084153969413 38-39 20.53780294198998 26.8585220333503 32.060946453357445 20.542728571302273 40-41 20.38866588208013 26.92144763036426 32.21877081610893 20.471115671446682 42-43 20.549184634348414 26.993174975246028 31.665816274981307 20.791824115424255 44-45 20.334076443900205 27.32484742490214 31.458381009104748 20.882695122092915 46-47 20.64300197921371 27.41999036156264 30.868358545828546 21.068649113395104 48-49 20.779420218693517 27.436408035264154 30.758991581016925 21.025180165025404 50-51 20.870500420797605 27.75324387974382 30.27671610154508 21.09953959791349 52-53 20.953498075848643 28.030020600391804 30.16679346162356 20.849687862135998 54-55 20.41699375724272 27.835660601725422 30.216147514566266 21.531198126465593 56-57 20.73971994472547 28.247700830507178 29.778005211728686 21.234574013038667 58-59 20.728601204097448 27.707223877460503 30.172122891086666 21.39205202735538 60-61 20.822535555629646 27.933282615350574 30.20089170700363 21.04329012201615 62-63 20.762143774367615 27.942400738744798 30.178816250364974 21.116639236522605 64-65 20.533736046240254 27.670498804288197 30.254615386946888 21.541149762524665 66-67 20.492278004468137 28.05264773076005 29.90463850149464 21.55043576327717 68-69 20.41957377826801 28.03082407599884 29.989090990835578 21.560511154897576 70-71 20.458719906404106 28.103950647967697 29.778528107562536 21.658801338065665 72-73 20.51896013990889 28.01079929318126 29.575782466328615 21.894458100581236 74-75 20.346498792164375 28.4014894113582 29.232072152897544 22.01993964357988 76-77 20.29065344361969 28.00882917233708 29.838894630865028 21.86162275317821 78-79 20.320331546080116 27.944396117548525 29.75728616358873 21.97798617278263 80-81 20.103896353196074 28.67298386669017 29.494652623879237 21.72846715623451 82-83 20.39477988121949 28.36554673108588 29.282252611537015 21.957420776157615 84-85 20.201219054758955 27.99943621685343 29.243143858918824 22.556200869468793 86-87 20.278160238423062 27.905616879762707 29.48565715747839 22.330565724335838 88-89 20.340133786360095 27.821970787654315 29.345051005923494 22.492844420062095 90-91 20.153966345941335 27.81967478537133 29.75347350006201 22.272885368625328 92-93 20.141303128854997 27.642588758995622 29.545144561405635 22.670963550743746 94-95 19.925857766837147 27.581673527151178 29.509462518062602 22.98300618794907 96-97 20.128083209884963 27.450396487120326 29.265939994620048 23.155580308374663 98-99 19.934718277770873 27.472122790054833 29.1873403065508 23.405818625623493 100-101 20.09472745146178 26.903086219560368 29.545169819365174 23.457016509612675 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6907.0 1 4426.5 2 2823.0 3 3661.0 4 2837.5 5 2510.5 6 5031.0 7 10576.0 8 15691.0 9 16228.5 10 11607.0 11 7113.0 12 6175.0 13 6170.0 14 6005.0 15 5746.5 16 5564.5 17 5565.5 18 5700.0 19 5882.0 20 6133.0 21 6709.5 22 7561.5 23 8535.5 24 9721.0 25 11285.0 26 13720.0 27 16561.5 28 18586.5 29 20903.0 30 24169.0 31 27492.5 32 31938.5 33 36501.0 34 41296.0 35 45116.0 36 49129.0 37 53822.0 38 55459.5 39 55539.5 40 55491.0 41 57286.0 42 59766.5 43 62485.5 44 64517.5 45 65966.5 46 65421.0 47 64594.5 48 72067.5 49 72917.0 50 65521.0 51 61003.5 52 58013.0 53 57615.5 54 57058.0 55 53748.0 56 47734.0 57 42355.5 58 38594.0 59 34097.0 60 29095.5 61 25015.0 62 21520.5 63 17116.5 64 13280.0 65 10555.5 66 7842.0 67 6608.5 68 5037.5 69 3061.0 70 2287.5 71 1916.0 72 1666.5 73 1511.0 74 1263.0 75 1077.0 76 756.5 77 389.5 78 184.0 79 85.5 80 46.5 81 23.5 82 15.0 83 8.5 84 3.0 85 1.5 86 2.0 87 3.0 88 1.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015457871238963534 3 0.002525795954079009 4 0.001464961653365825 5 0.12048046700956873 6 5.051591908158018E-5 7 0.0 8 1.0103183816316037E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.030955144894811E-4 24-25 3.536114335710612E-4 26-27 0.0056830408966777705 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010153699735397616 36-37 6.061910289789622E-4 38-39 0.006642843359227793 40-41 0.009471734827796284 42-43 0.004748496393668537 44-45 0.00287940738765007 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.525795954079009E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 7.577387862237027E-5 68-69 8.840285839276531E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.525795954079009E-4 82-83 0.002045894722803997 84-85 0.004521174757801426 86-87 0.003409824538006662 88-89 0.0 90-91 0.002551053913619799 92-93 0.0 94-95 2.525795954079009E-5 96-97 7.577387862237027E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1979574.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.14629114197726 #Duplication Level Percentage of deduplicated Percentage of total 1 70.43475787755831 29.685638120222492 2 14.960438548080754 12.61053997318142 3 5.712676952279253 7.223044380924741 4 2.812868359088034 4.742078752247204 5 1.5919017053206201 3.3546376370926465 6 0.9927062463352726 2.510333188590348 7 0.6292434052938435 1.8564193031078466 8 0.4424162521135161 1.4916963334014928 9 0.3384707141776329 1.2838756736487138 >10 1.783490536470432 13.881814874768668 >50 0.17767186411307861 5.242514404925657 >100 0.11452146189244677 8.924500026410511 >500 0.0062091353836700065 1.777140494490173 >1k 0.002029909644661348 1.7239465573373933 >5k 2.3881289937192332E-4 0.5931853710688888 >10k+ 3.58219349057885E-4 3.0986349085817952 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26248 1.3259418440533166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20555 1.0383547167218807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14769 0.7460696089158576 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6711 0.33901233295648453 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.25641880525810096 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4478 0.22621028564731605 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3114 0.15730657202004067 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2623 0.13250325575098482 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2588 0.1307351985831295 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2504 0.12649186138027677 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.11835879840814235 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2089 0.10552775496142099 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5257959540790088E-5 0.0 14-15 0.0 0.0 0.0 5.0515919081580176E-5 0.0 16-17 0.0 0.0 0.0 1.5154775724474052E-4 0.0 18-19 0.0 0.0 0.0 3.283534740302712E-4 0.0 20-21 0.0 0.0 0.0 6.567069480605424E-4 0.0 22-23 0.0 0.0 0.0 0.0012881559365802947 0.0 24-25 0.0 0.0 0.0 0.0029299233067316506 0.0 26-27 0.0 0.0 0.0 0.00583458865392251 0.0 28-29 0.0 0.0 0.0 0.013588782232945067 0.0 30-31 0.0 0.0 0.0 0.03384566578465872 0.0 32-33 0.0 0.0 0.0 0.06794391116472534 0.0 34-35 0.0 0.0 0.0 0.11765157554100024 0.0 36-37 0.0 0.0 0.0 0.19542083296709292 0.0 38-39 0.0 0.0 0.0 0.30791978476177195 0.0 40-41 0.0 0.0 0.0 0.42703632195613805 0.0 42-43 0.0 0.0 0.0 0.543273451762854 0.0 44-45 0.0 0.0 0.0 0.6814597484105167 0.0 46-47 0.0 0.0 0.0 0.8387915783900981 0.0 48-49 0.0 0.0 0.0 1.008474550585126 0.0 50-51 0.0 0.0 0.0 1.1865936812667774 0.0 52-53 0.0 0.0 0.0 1.3528920868833396 0.0 54-55 0.0 0.0 0.0 1.5230044443905608 0.0 56-57 0.0 0.0 0.0 1.71958714349653 0.0 58-59 0.0 0.0 0.0 1.935820535125234 0.0 60-61 0.0 0.0 0.0 2.168799953929482 0.0 62-63 0.0 0.0 0.0 2.3900849374663435 0.0 64-65 0.0 0.0 0.0 2.6128853985756533 0.0 66-67 0.0 0.0 0.0 2.860741755549426 0.0 68-69 0.0 0.0 0.0 3.1265060058376197 0.0 70-71 0.0 0.0 0.0 3.417048314435328 0.0 72-73 0.0 0.0 0.0 3.7026400629630416 0.0 74-75 0.0 0.0 0.0 3.9824224807963735 0.0 76-77 0.0 0.0 0.0 4.286124186314833 0.0 78-79 0.0 0.0 0.0 4.612835892974953 0.0 80-81 0.0 0.0 0.0 4.963088017927089 0.0 82-83 0.0 0.0 0.0 5.321018562579626 0.0 84-85 0.0 0.0 0.0 5.67407432104079 0.0 86-87 0.0 0.0 0.0 6.038142549861738 0.0 88-89 0.0 0.0 0.0 6.41190983514635 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11745 0.0 40.0893 1 GTATCAA 18920 0.0 34.84082 1 TATCAAC 23185 0.0 28.411161 2 ATCAACG 23265 0.0 28.185104 3 TCAACGC 23445 0.0 27.988993 4 CAACGCA 23750 0.0 27.629559 5 AACGCAG 24255 0.0 27.081606 6 CATTCCG 320 0.0 26.716286 9 CGGCGAT 45 8.954929E-4 26.387123 82-83 GTACATG 15195 0.0 25.384373 1 TACATGG 15510 0.0 24.562187 2 GAGTACT 15275 0.0 24.424097 12-13 ACGCAGA 27030 0.0 24.178308 7 CGCAGAG 27115 0.0 24.085 8 ATAGCGT 120 1.7124951E-4 23.74781 6 ACATGGG 15615 0.0 23.508976 3 GCAGAGT 27820 0.0 23.286854 9 GTACTTT 16085 0.0 23.031757 14-15 CATGGGG 4985 0.0 22.606932 4 AGTACTT 15380 0.0 22.46623 12-13 >>END_MODULE