FastQCFastQC Report
Thu 26 May 2016
SRR938030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989615
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275341.3838858271575154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208841.0496503092306804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148700.747380774672487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69530.3494645949090653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49340.24798767600766985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.21607195361916753No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.16425288309547323No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.14635997416585622No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26890.13515177559477587No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.12761262857386982No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.11751017156585572No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21720.10916684886271967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118050.042.592571
GTATCAA187450.034.9494361
TATCAAC228850.028.5932162
ATCAACG230850.028.325623
TCAACGC233200.028.0605564
CAACGCA235300.027.8505135
AACGCAG240800.027.2518066
GTACATG152800.027.187381
TACATGG157050.025.9312132
ACATGGG156000.025.4362323
GAGTACT149550.025.12437612-13
ACGCAGA268150.024.3909447
CATGGGG49400.024.2417774
CGCAGAG269450.024.1851278
GTACTTT157850.023.70154814-15
GCAGAGT278350.023.0358439
CATGGGA102550.022.7992384
AGTACTT152300.022.26914412-13
ACTTTTT175200.021.62501316-17
ATGGGAT37900.020.8142015
Produced by FastQC (version 0.11.5)