##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938030_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989615 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.018442764052345 33.0 31.0 34.0 30.0 34.0 2 32.18076110202225 34.0 31.0 34.0 30.0 34.0 3 32.239086456424985 34.0 31.0 34.0 30.0 34.0 4 35.7681581612523 37.0 35.0 37.0 35.0 37.0 5 35.79414208276476 37.0 35.0 37.0 35.0 37.0 6 35.84990664022939 37.0 36.0 37.0 35.0 37.0 7 35.80348409114326 37.0 36.0 37.0 35.0 37.0 8 35.78874154044878 37.0 36.0 37.0 35.0 37.0 9 37.4312291573998 39.0 38.0 39.0 35.0 39.0 10-11 37.362436953883034 39.0 37.0 39.0 35.0 39.0 12-13 37.25882042505711 39.0 37.0 39.0 34.0 39.0 14-15 38.42183336977254 40.0 38.0 41.0 34.0 41.0 16-17 38.311325055349904 40.0 38.0 41.0 34.0 41.0 18-19 38.509583512388076 40.0 38.0 41.0 34.0 41.0 20-21 38.52591255092065 40.0 38.0 41.0 34.0 41.0 22-23 38.43189109450823 40.0 38.0 41.0 34.0 41.0 24-25 38.323692272123 40.0 38.0 41.0 34.0 41.0 26-27 38.14636726200798 40.0 38.0 41.0 34.0 41.0 28-29 37.93577023695539 40.0 38.0 41.0 33.0 41.0 30-31 37.665088471890286 40.0 37.0 41.0 33.0 41.0 32-33 37.357556612711505 40.0 36.0 41.0 33.0 41.0 34-35 37.11458322338744 40.0 35.5 41.0 32.0 41.0 36-37 36.84886975620911 39.0 35.0 41.0 31.0 41.0 38-39 36.57546862081358 39.0 35.0 41.0 31.0 41.0 40-41 36.34034323223337 39.0 35.0 41.0 30.0 41.0 42-43 36.125524787458886 39.0 35.0 41.0 30.0 41.0 44-45 35.78680322574971 39.0 35.0 41.0 28.5 41.0 46-47 35.28403962575675 39.0 35.0 40.0 26.5 41.0 48-49 34.81956785609276 38.0 35.0 40.0 24.0 41.0 50-51 34.086190795706706 37.5 33.5 39.5 23.0 40.5 52-53 34.20060589611558 38.0 34.0 40.0 23.0 40.5 54-55 34.329566775481695 38.0 34.0 40.0 23.0 41.0 56-57 34.08310929501437 38.0 34.0 40.0 22.0 41.0 58-59 33.811499460950984 38.0 34.0 40.0 20.5 41.0 60-61 33.44770345016498 37.0 33.5 40.0 19.0 41.0 62-63 32.969280488938814 36.5 33.0 40.0 16.5 41.0 64-65 32.50469412424012 36.0 32.5 39.0 10.5 41.0 66-67 32.02482791896925 35.5 31.5 39.0 7.5 40.0 68-69 31.486706724667837 35.0 31.0 38.5 4.5 40.0 70-71 31.02349751082496 35.0 31.0 38.0 2.0 40.0 72-73 30.447541609808933 35.0 30.0 37.0 2.0 39.0 74-75 29.81563217004295 34.5 29.0 36.5 2.0 39.0 76-77 29.195500637057922 34.0 29.0 36.0 2.0 38.5 78-79 28.519318561631273 34.0 27.5 35.5 2.0 37.5 80-81 27.901591262631214 34.0 26.5 35.0 2.0 37.0 82-83 27.22112896213589 33.0 25.5 35.0 2.0 36.5 84-85 26.605880032066505 33.0 24.5 35.0 2.0 36.0 86-87 25.955905790818825 32.0 23.0 35.0 2.0 35.5 88-89 25.193807595941927 32.0 19.0 34.5 2.0 35.0 90-91 24.41759134304878 31.0 14.5 34.0 2.0 35.0 92-93 23.583118593295687 31.0 4.5 34.0 2.0 35.0 94-95 22.696476202682426 30.5 2.0 34.0 2.0 35.0 96-97 21.547816537370295 29.5 2.0 34.0 2.0 35.0 98-99 20.144188699823836 29.0 2.0 34.0 2.0 35.0 100-101 16.43665281976664 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36105586569106407 1101 2 0.30750728423418394 1101 3 0.2450022006469652 1101 4 0.1879740717029108 1101 5 0.20881132914791323 1101 6 0.283686885565011 1101 7 0.17406949079074963 1101 8 0.1729418388401669 1101 9 0.299597824245474 1101 10-11 0.39353560581262315 1101 12-13 0.4325012046877674 1101 14-15 0.49494625541936443 1101 16-17 0.5499011715106832 1101 18-19 0.48157307982999953 1101 20-21 0.49183834068222865 1101 22-23 0.5128808551789277 1101 24-25 0.5577466760720782 1101 26-27 0.5723035255802955 1101 28-29 0.6265481783369893 1101 30-31 0.46144017136874993 1101 32-33 0.47887888222232533 1101 34-35 0.6144065809953716 1101 36-37 0.6433101594982631 1101 38-39 0.6543429321903389 1101 40-41 0.859255844382325 1101 42-43 0.8297587308617693 1101 44-45 0.6699123320798961 1101 46-47 0.904289494443347 1101 48-49 0.6857924367361719 1101 50-51 0.7502756784160454 1101 52-53 0.7331872315754637 1101 54-55 0.8723666457343953 1101 56-57 0.7217984528657766 1101 58-59 0.8722605992858448 1101 60-61 0.7867579437159691 1101 62-63 0.8658492256606394 1101 64-65 0.9899240791119084 1101 66-67 1.0122837787675465 1101 68-69 1.2509856092932843 1101 70-71 1.3437049428545969 1101 72-73 1.5209273888145383 1101 74-75 1.8009487502316865 1101 76-77 1.8029760957591527 1101 78-79 1.7928239859073578 1101 80-81 1.8970327265079945 1101 82-83 2.012181093454231 1101 84-85 2.096963741428933 1101 86-87 2.1079164100569336 1101 88-89 2.2211786158323648 1101 90-91 2.7198585824440222 1101 92-93 2.8345950790568075 1101 94-95 2.8745933304400566 1101 96-97 3.3115994911195976 1101 98-99 3.7103315274362707 1101 100-101 3.8467054317033593 1102 1 0.22589571559775123 1102 2 0.3019674824681431 1102 3 0.1256737297390913 1102 4 0.10061692743263251 1102 5 0.24188065827828353 1102 6 0.22429286423804484 1102 7 0.10943275485870174 1102 8 0.23430896734932105 1102 9 0.332888402526784 1102 10-11 0.35851262359827984 1102 12-13 0.4008358679340276 1102 14-15 0.4137760089788003 1102 16-17 0.47738189876422865 1102 18-19 0.3008003234810417 1102 20-21 0.37303578988724695 1102 22-23 0.4812202995171262 1102 24-25 0.3357638748138214 1102 26-27 0.14082646492966688 1102 28-29 0.167306162760525 1102 30-31 0.19814263577434588 1102 32-33 0.23851344193321466 1102 34-35 0.3288158028973882 1102 36-37 0.28110584530468685 1102 38-39 0.2889316593647493 1102 40-41 0.3867934501433723 1102 42-43 0.49759418216386564 1102 44-45 0.4106325623636664 1102 46-47 0.36060284391148656 1102 48-49 0.3236234037037704 1102 50-51 0.3244312386610986 1102 52-53 0.24571731948160647 1102 54-55 0.48645685339864997 1102 56-57 0.595032086693486 1102 58-59 0.4374270472313029 1102 60-61 0.5211224541651376 1102 62-63 0.5203480683066601 1102 64-65 0.5085594565543872 1102 66-67 0.6181434190975423 1102 68-69 0.6464877558387094 1102 70-71 0.7585579819804202 1102 72-73 1.0270862595535988 1102 74-75 0.9770028985708343 1102 76-77 1.180233100883168 1102 78-79 1.4531668066856547 1102 80-81 1.3663321811337816 1102 82-83 1.5145543285718475 1102 84-85 1.6365277717905151 1102 86-87 1.7162366640812934 1102 88-89 1.9689865676125784 1102 90-91 2.148331633572031 1102 92-93 2.286025779602184 1102 94-95 2.716235322275935 1102 96-97 3.0877030990304917 1102 98-99 3.3942762909865465 1102 100-101 3.411474059826734 1103 1 0.4629031063887439 1103 2 0.39025912236503757 1103 3 0.43245961458835325 1103 4 0.37010428561764996 1103 5 0.3365857655622051 1103 6 0.40807520380997175 1103 7 0.40635751066392345 1103 8 0.35276657725058413 1103 9 0.521337627590448 1103 10-11 0.48942148399151364 1103 12-13 0.5622751043071119 1103 14-15 0.8059746487372479 1103 16-17 0.7388157682189984 1103 18-19 0.588960279425649 1103 20-21 0.6689575542188706 1103 22-23 0.5644135614678589 1103 24-25 0.6418520602820763 1103 26-27 0.7248727946575713 1103 28-29 0.8433986417634287 1103 30-31 0.8894295216705572 1103 32-33 1.0490781428558549 1103 34-35 1.080005469790656 1103 36-37 1.1510311906931037 1103 38-39 1.1417178677847417 1103 40-41 1.112362887954795 1103 42-43 1.3372438857388573 1103 44-45 1.3656898717174997 1103 46-47 1.5683414734338896 1103 48-49 1.792018866246572 1103 50-51 1.8482877739341959 1103 52-53 1.8453409351666963 1103 54-55 1.8958234092527135 1103 56-57 1.9800021097323466 1103 58-59 1.9207890170925523 1103 60-61 2.010380839391644 1103 62-63 2.0486453395787123 1103 64-65 2.2358737261790296 1103 66-67 2.42737241850174 1103 68-69 2.5018528074000095 1103 70-71 2.7342785102096308 1103 72-73 2.710534333452177 1103 74-75 2.710870836600815 1103 76-77 2.8995590237208795 1103 78-79 3.0699655332481655 1103 80-81 3.1723787535397356 1103 82-83 3.098193666994163 1103 84-85 2.9730191606089704 1103 86-87 3.1099158732619934 1103 88-89 3.5399345053936635 1103 90-91 3.804609100137686 1103 92-93 4.11553278537512 1103 94-95 4.2821803588098675 1103 96-97 4.4125729707066945 1103 98-99 4.7395184790091385 1103 100-101 4.8276494071596865 1104 1 0.22795444585192115 1104 2 0.23851762234980356 1104 3 0.12528792044736292 1104 4 0.11365633833810307 1104 5 0.15673145707151548 1104 6 0.1549636124074425 1104 7 0.10670084781133227 1104 8 0.19781270518999605 1104 9 0.1844921195001561 1104 10-11 0.26604955105906214 1104 12-13 0.2441047484209875 1104 14-15 0.3827546135104285 1104 16-17 0.4474234683154137 1104 18-19 0.37736033112059886 1104 20-21 0.48793975903665654 1104 22-23 0.410610567573535 1104 24-25 0.4211857067271154 1104 26-27 0.4746452282885656 1104 28-29 0.3795684729948121 1104 30-31 0.42354206186460175 1104 32-33 0.39554710784653224 1104 34-35 0.46328257260372396 1104 36-37 0.5181000342916988 1104 38-39 0.48832167394827763 1104 40-41 0.5754790989808782 1104 42-43 0.5513151049937619 1104 44-45 0.6145271730588036 1104 46-47 0.4757420123082525 1104 48-49 0.5773541707126881 1104 50-51 0.7009762442433995 1104 52-53 0.6646434315496279 1104 54-55 0.7533167783942574 1104 56-57 0.8595526928591326 1104 58-59 0.7576945907962127 1104 60-61 0.8735033897040339 1104 62-63 0.8276523901959791 1104 64-65 0.842235567738193 1104 66-67 0.8909064620452725 1104 68-69 0.9823325337669964 1104 70-71 1.1703447063681658 1104 72-73 1.228482394033339 1104 74-75 1.2503596869870073 1104 76-77 1.201153897094514 1104 78-79 1.4774443500501455 1104 80-81 1.343575032904024 1104 82-83 1.6807133126525748 1104 84-85 1.7830507134434157 1104 86-87 1.7776276465775176 1104 88-89 2.1669904392633477 1104 90-91 2.3917170231598384 1104 92-93 2.486450906662828 1104 94-95 2.6623189895450388 1104 96-97 2.727427809806155 1104 98-99 2.947136584991501 1104 100-101 3.359759993732794 1105 1 0.37383621903024533 1105 2 0.33353969945562767 1105 3 0.2780159023060591 1105 4 0.25041501600444604 1105 5 0.19536026522403915 1105 6 0.3466983023858461 1105 7 0.32560586025646643 1105 8 0.3128727040568222 1105 9 0.44896728259139707 1105 10-11 0.31938891919661216 1105 12-13 0.3497657373646419 1105 14-15 0.4463005748692339 1105 16-17 0.538608174171415 1105 18-19 0.3663273123086128 1105 20-21 0.3663727127570908 1105 22-23 0.44416618725437473 1105 24-25 0.4004495660239513 1105 26-27 0.3612935533668775 1105 28-29 0.5105690514400223 1105 30-31 0.46426526331506324 1105 32-33 0.3925348445446062 1105 34-35 0.45234521466209543 1105 36-37 0.49563000737226304 1105 38-39 0.5501108221053599 1105 40-41 0.5961183929351392 1105 42-43 0.5325248592401906 1105 44-45 0.6618608568851343 1105 46-47 0.603811889543465 1105 48-49 0.7793307667405216 1105 50-51 0.7605161760685561 1105 52-53 0.6146969937311653 1105 54-55 0.7202332701438436 1105 56-57 0.655252170150213 1105 58-59 0.8601185265690319 1105 60-61 1.0375042013720233 1105 62-63 1.0070286444272654 1105 64-65 0.9701491725323095 1105 66-67 1.1329166052598723 1105 68-69 1.2208382740780372 1105 70-71 1.1453529745770048 1105 72-73 1.1529342875519824 1105 74-75 1.321581576724018 1105 76-77 1.4833795539724086 1105 78-79 1.7302599551578588 1105 80-81 1.6761316157817845 1105 82-83 1.8318211208722914 1105 84-85 1.958865906128917 1105 86-87 2.1028263800592164 1105 88-89 2.2986338956513457 1105 90-91 2.505422089891862 1105 92-93 2.700724931574186 1105 94-95 2.966639145132305 1105 96-97 3.2590283110374934 1105 98-99 3.432397168628224 1105 100-101 3.801237150417659 1106 1 0.202216058961028 1106 2 0.09288873433239075 1106 3 0.16324958046259042 1106 4 0.2184915133133032 1106 5 0.1366118080215557 1106 6 0.07845511932417537 1106 7 0.16775954197574805 1106 8 0.2275219641502133 1106 9 0.047618527644900155 1106 10-11 0.1103535334654211 1106 12-13 0.2676704592793655 1106 14-15 0.3830045231584762 1106 16-17 0.18007666292967173 1106 18-19 0.2619801224268201 1106 20-21 0.1761136500145355 1106 22-23 0.22821843242240192 1106 24-25 0.190883585186441 1106 26-27 0.17247485911434524 1106 28-29 0.22901136671785594 1106 30-31 0.13284754372885033 1106 32-33 0.11985985631439888 1106 34-35 0.2845371959322165 1106 36-37 0.3839104720565132 1106 38-39 0.3174811316827899 1106 40-41 0.6114114926539358 1106 42-43 0.43691531107310055 1106 44-45 0.5735562164046755 1106 46-47 0.9646540979388334 1106 48-49 1.036207508352156 1106 50-51 0.8409598655495429 1106 52-53 0.9672367247436213 1106 54-55 0.4002144384710036 1106 56-57 0.7722350307831078 1106 58-59 0.9857321464491662 1106 60-61 1.0765023054806377 1106 62-63 0.9549075364123496 1106 64-65 1.0961057069955444 1106 66-67 1.2979412263997077 1106 68-69 1.0405141911677767 1106 70-71 1.1521762749549076 1106 72-73 1.4706621444312695 1106 74-75 1.4082479889086201 1106 76-77 1.436364628599275 1106 78-79 1.815204159965024 1106 80-81 1.8588102940019375 1106 82-83 1.9148528929581552 1106 84-85 2.188082355849623 1106 86-87 2.0931458822923013 1106 88-89 2.4054603770550678 1106 90-91 2.6028664537503197 1106 92-93 2.848764147260461 1106 94-95 3.010196295442139 1106 96-97 3.0273478387187787 1106 98-99 3.520367801397878 1106 100-101 3.7470222912098627 1107 1 0.2744951077326121 1107 2 0.16886887225282976 1107 3 0.12785272305080753 1107 4 0.10050759122490405 1107 5 0.18899948354356155 1107 6 0.14459449964265758 1107 7 0.1317859351066204 1107 8 0.28732046200841665 1107 9 0.2603819307068136 1107 10-11 0.2009978024679029 1107 12-13 0.24070249984871595 1107 14-15 0.3877683488178292 1107 16-17 0.3136796422416097 1107 18-19 0.2522186018444046 1107 20-21 0.25352413619367553 1107 22-23 0.2776390417310637 1107 24-25 0.2786688384567739 1107 26-27 0.42895332585401036 1107 28-29 0.3999388240259094 1107 30-31 0.4519451061099957 1107 32-33 0.4729755519674015 1107 34-35 0.5945011310827155 1107 36-37 0.5806108504733132 1107 38-39 0.515866137157353 1107 40-41 0.4300994236552995 1107 42-43 0.5900362891940745 1107 44-45 0.52766813749421 1107 46-47 0.6766292127124558 1107 48-49 0.6185743901593455 1107 50-51 0.650678886276026 1107 52-53 0.6219299574219335 1107 54-55 0.8083928994479734 1107 56-57 0.7549130979761074 1107 58-59 0.761961760925054 1107 60-61 0.911145690870292 1107 62-63 0.912220633091124 1107 64-65 0.9443216734950894 1107 66-67 1.1188093759991986 1107 68-69 1.2312470615648259 1107 70-71 1.2779182215923761 1107 72-73 1.2952830072984014 1107 74-75 1.4119627644517543 1107 76-77 1.505773376366232 1107 78-79 1.5861157698691706 1107 80-81 1.7141936090867453 1107 82-83 1.7154783851881241 1107 84-85 1.8845795993617926 1107 86-87 1.8748774272701105 1107 88-89 2.0244796628653994 1107 90-91 2.4305316989528727 1107 92-93 2.6732493467456564 1107 94-95 2.828097859052658 1107 96-97 2.9010711622555334 1107 98-99 2.9472800548026683 1107 100-101 3.005754450874857 1108 1 0.29494256726458445 1108 2 0.12812803080776547 1108 3 0.19330353603682937 1108 4 0.1448316436475423 1108 5 0.15579756935573386 1108 6 0.17414568060228675 1108 7 0.2019827919231929 1108 8 0.1819540291771915 1108 9 0.2357412940943462 1108 10-11 0.2641759705756428 1108 12-13 0.35192524170526696 1108 14-15 0.3825840901124451 1108 16-17 0.3243447590686941 1108 18-19 0.3287974442683179 1108 20-21 0.3029828252650404 1108 22-23 0.2540925827972771 1108 24-25 0.2190596064883863 1108 26-27 0.15498134079047077 1108 28-29 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1274.0 5 1399.0 6 1645.0 7 2074.0 8 2585.0 9 3295.0 10 4160.0 11 5580.0 12 7980.0 13 10517.0 14 12462.0 15 16019.0 16 21453.0 17 29066.0 18 27733.0 19 17885.0 20 16856.0 21 17729.0 22 18639.0 23 20882.0 24 23551.0 25 26409.0 26 30588.0 27 35480.0 28 41396.0 29 48940.0 30 58941.0 31 71482.0 32 89281.0 33 114991.0 34 156933.0 35 229625.0 36 278031.0 37 332485.0 38 193965.0 39 11833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.24795589964908 19.23416242006534 14.65904390996717 20.858837770318416 2 15.44091471324705 21.075361269496483 40.91773011132229 22.565993905934175 3 18.652496428357708 27.31173635874023 30.416311932981355 23.619455279920707 4 11.952758835717017 17.629191559551828 37.54018063685555 32.87786896787561 5 12.525139012830328 39.020878836749084 33.28274510474631 15.17123704567428 6 29.46683139131292 37.22959771907338 17.801730816050995 15.5018400735627 7 27.162473513087328 35.61369686400988 20.340339857134094 16.8834897657687 8 26.78598724064971 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20.181123119219695 32-33 20.191996564647802 27.05875372441808 32.32248061391835 20.426769097015764 34-35 20.775905484133837 27.058759636400847 31.98599932566251 20.179335553802805 36-37 21.041438297615574 26.674904713537213 31.92202097810674 20.36163601074047 38-39 20.572097358506504 26.88962154781404 32.05692820177145 20.481352891908006 40-41 20.45496534809884 26.915846316537216 32.292037691656276 20.337150643707673 42-43 20.55426647995389 27.03715629503333 31.725914290590733 20.682662934422037 44-45 20.382477621738897 27.359965897877615 31.51774551994929 20.7398109604342 46-47 20.58488907926185 27.498066943578888 30.961078449412238 20.955965527747026 48-49 20.807782463064093 27.488509178203575 30.809730592545577 20.893977766186755 50-51 20.95878639113681 27.8451560883152 30.273514915876465 20.922542604671527 52-53 20.9159370196218 28.009222256069666 30.32230141184064 20.752539312467892 54-55 20.46801866585298 27.868205967824878 30.32095193236217 21.342823433959968 56-57 20.74484395641517 28.18519028651054 29.98246633516244 21.087499421911854 58-59 20.848925741806557 27.597463391927864 30.304906201750875 21.248704664514705 60-61 20.938671350413625 27.735567027540725 30.450705917511794 20.87505570453385 62-63 20.87431106142286 27.734239116228288 30.452015088785355 20.939434733563502 64-65 20.676768077905063 27.521988967939865 30.52953989181081 21.271703062344262 66-67 20.634559599321815 27.922286165815585 30.169440748517033 21.273713486345567 68-69 20.630704989552008 27.929145657157996 30.20752225445453 21.232627098835465 70-71 20.651613840516656 28.07401844202445 30.029794984167484 21.24457273329141 72-73 20.85205637144631 27.99143419435431 29.754185506998677 21.402323927200705 74-75 20.692635687652924 28.419653078946343 29.524406749415327 21.363304483985406 76-77 20.80303060386134 28.082496644153615 29.732283757452166 21.382188994532882 78-79 20.852558827624794 28.049181407399175 29.656054880816598 21.442204884159434 80-81 20.811051290171772 28.824719620765404 29.272371271873602 21.09185781718922 82-83 21.11616560336336 28.484313354390782 29.10002641606841 21.299494626177445 84-85 21.028421085173278 28.167057539634165 29.025955958992643 21.77856541619991 86-87 21.181794581238325 28.052542521539415 29.19257390048657 21.573088996735695 88-89 21.30684517599978 27.927870632684197 29.270184735038825 21.495099456277195 90-91 21.195662557076247 28.082469333928607 29.341842106056998 21.380026002938152 92-93 21.302615294440553 27.81830575409014 29.246347203888007 21.632731747581307 94-95 21.25102407683511 27.7866642131558 29.118774813202602 21.84353689680649 96-97 21.581899771856847 27.651397464785614 28.755639479523925 22.01106328383361 98-99 21.50705453850775 27.61606117201109 28.678603118425254 22.198281171055907 100-101 22.282948868728514 26.827413869517517 28.860101058021154 22.02953620373281 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7275.0 1 4670.0 2 2901.5 3 3644.0 4 2792.5 5 2574.5 6 5003.0 7 10584.0 8 16216.0 9 16889.5 10 11929.0 11 7283.5 12 6294.5 13 6239.0 14 6203.5 15 6060.0 16 5867.0 17 5752.0 18 5793.0 19 6007.5 20 6324.0 21 6930.5 22 7808.5 23 8916.5 24 10010.5 25 11547.0 26 14294.5 27 17076.0 28 18871.5 29 21220.0 30 24502.0 31 27891.5 32 32260.0 33 37037.0 34 41776.5 35 45222.0 36 49191.5 37 53622.5 38 55392.5 39 55618.5 40 54941.0 41 56856.0 42 59956.5 43 62468.0 44 64331.5 45 65882.0 46 65451.0 47 64767.5 48 71918.5 49 72372.0 50 64805.0 51 60245.5 52 57738.5 53 57849.0 54 57149.0 55 53280.0 56 47401.0 57 42598.0 58 38924.5 59 34322.0 60 29367.5 61 25500.0 62 21800.5 63 17348.5 64 13546.5 65 10793.0 66 8265.5 67 6793.0 68 5209.5 69 3398.0 70 2561.5 71 2115.0 72 1845.5 73 1610.0 74 1328.5 75 1165.0 76 806.5 77 421.5 78 247.5 79 144.0 80 75.0 81 48.5 82 30.5 83 16.5 84 12.5 85 10.5 86 11.5 87 10.0 88 8.5 89 5.0 90 2.0 91 2.0 92 1.5 93 0.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4726542572306702 2 0.039957479210802095 3 0.0519698534641124 4 0.0550357732526142 5 0.0838855758526147 6 0.08298087821010597 7 0.045084099185018205 8 0.040158523131359584 9 0.04553644800627257 10-11 0.04759714819198689 12-13 0.0439532271318823 14-15 0.03849991078676025 16-17 0.03819834490592401 18-19 0.04593853584738756 20-21 0.05644308069651666 22-23 0.04684323348989629 24-25 0.06737484387683045 26-27 0.05679490755749228 28-29 0.04417940154250948 30-31 0.0458631443771785 32-33 0.04430505399285791 34-35 0.05008506670888589 36-37 0.043978357621951986 38-39 0.025783882811498706 40-41 0.023848835076132818 42-43 0.023295964294599708 44-45 0.014877250121254614 46-47 0.02699014633484368 48-49 0.026512667023519624 50-51 0.015505512372996787 52-53 0.014927511101393988 54-55 0.01578194776376334 56-57 0.015028033061672736 58-59 0.013344290227003716 60-61 0.016309688055226767 62-63 0.016234296585017703 64-65 0.01618403560487833 66-67 0.009449064266202255 68-69 0.01196211327317094 70-71 0.01779238696933829 72-73 0.015580903843205846 74-75 0.012942202385888727 76-77 0.008871062994599457 78-79 0.014374640319860877 80-81 0.017340038148083926 82-83 0.014852119631184928 84-85 0.013294029246864342 86-87 0.0032418332189896033 88-89 0.027643539076655534 90-91 0.030608936904878585 92-93 0.015932730704181462 94-95 0.03158902601759637 96-97 0.025783882811498706 98-99 0.023622660665505637 100-101 0.1474908462189921 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989615.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.38556419697793 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6444328156379 31.08334139278931 2 14.45359178951592 12.541544689219123 3 5.409104417240236 7.040311408269995 4 2.728623839632205 4.7353153905506975 5 1.5087397758903986 3.2728763201713496 6 0.9567585926972957 2.490570680668727 7 0.6072982175614228 1.8443583063304965 8 0.4314996189262687 1.4976683534317714 9 0.31936623259758146 1.2470295768038422 >10 1.656654460208765 13.41720961458488 >50 0.16936414522759818 5.1321155806697165 >100 0.10689026032600885 8.66376300593172 >500 0.005233523548899316 1.5531870191214256 >1k 0.0019771088962508526 1.9365136965389254 >5k 1.1630052330887369E-4 0.35083195841567383 >10k+ 3.48901569926621E-4 3.193363006502397 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27534 1.3838858271575154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20884 1.0496503092306804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14870 0.747380774672487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6953 0.3494645949090653 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4934 0.24798767600766985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.21607195361916753 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3268 0.16425288309547323 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.14635997416585622 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2689 0.13515177559477587 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2539 0.12761262857386982 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2338 0.11751017156585572 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2172 0.10916684886271967 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.539147020906055E-5 0.0 14-15 0.0 0.0 0.0 1.005219602787474E-4 0.0 16-17 0.0 0.0 0.0 1.005219602787474E-4 0.0 18-19 0.0 0.0 0.0 2.010439205574948E-4 0.0 20-21 0.0 0.0 0.0 3.2669637090592903E-4 0.0 22-23 5.02609801393737E-5 0.0 0.0 0.0010303500928571607 0.0 24-25 5.02609801393737E-5 0.0 0.0 0.0026387014573171193 0.0 26-27 5.02609801393737E-5 0.0 0.0 0.00510148948414643 0.0 28-29 5.02609801393737E-5 0.0 0.0 0.01236420111428593 0.0 30-31 5.02609801393737E-5 0.0 0.0 0.03234294071968698 0.0 32-33 5.02609801393737E-5 0.0 0.0 0.06719893044634263 0.0 34-35 5.02609801393737E-5 0.0 0.0 0.11776147646655258 0.0 36-37 5.02609801393737E-5 0.0 0.0 0.1961937359740452 0.0 38-39 5.02609801393737E-5 0.0 0.0 0.30832598266498795 0.0 40-41 5.02609801393737E-5 0.0 0.0 0.4306109473440841 0.0 42-43 5.02609801393737E-5 0.0 0.0 0.5420395403130757 0.0 44-45 5.02609801393737E-5 0.0 0.0 0.6725673057350292 0.0 46-47 5.02609801393737E-5 0.0 0.0 0.8272203416238819 0.0 48-49 5.02609801393737E-5 0.0 0.0 0.9934585334348605 0.0 50-51 5.02609801393737E-5 0.0 0.0 1.172689188611867 0.0 52-53 5.02609801393737E-5 0.0 0.0 1.3426215624630897 0.0 54-55 5.02609801393737E-5 0.0 0.0 1.5165496842353923 0.0 56-57 5.02609801393737E-5 0.0 0.0 1.7076419307252912 0.0 58-59 5.02609801393737E-5 0.0 0.0 1.9225327513111834 0.0 60-61 5.02609801393737E-5 0.0 0.0 2.149762642521292 0.0 62-63 5.02609801393737E-5 0.0 0.0 2.3700565184721665 0.0 64-65 5.02609801393737E-5 5.02609801393737E-5 0.0 2.5923357031385468 0.0 66-67 5.02609801393737E-5 5.02609801393737E-5 0.0 2.840624945027053 0.0 68-69 5.02609801393737E-5 5.02609801393737E-5 0.0 3.111782932878974 0.0 70-71 5.02609801393737E-5 5.02609801393737E-5 0.0 3.403975140919223 0.0 72-73 5.02609801393737E-5 5.02609801393737E-5 0.0 3.6911663814356044 0.0 74-75 5.02609801393737E-5 5.02609801393737E-5 0.0 3.975065527752857 0.0 76-77 5.02609801393737E-5 5.02609801393737E-5 0.0 4.272082789886486 0.0 78-79 5.02609801393737E-5 5.02609801393737E-5 0.0 4.596291242275516 0.0 80-81 5.02609801393737E-5 5.02609801393737E-5 0.0 4.952716982933884 0.0 82-83 5.02609801393737E-5 5.02609801393737E-5 0.0 5.301854881472043 0.0 84-85 5.02609801393737E-5 5.02609801393737E-5 0.0 5.6552649633220495 0.0 86-87 5.02609801393737E-5 5.02609801393737E-5 0.0 6.020360723054461 0.0 88-89 5.02609801393737E-5 5.02609801393737E-5 0.0 6.393850066470146 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11805 0.0 42.59257 1 GTATCAA 18745 0.0 34.949436 1 TATCAAC 22885 0.0 28.593216 2 ATCAACG 23085 0.0 28.32562 3 TCAACGC 23320 0.0 28.060556 4 CAACGCA 23530 0.0 27.850513 5 AACGCAG 24080 0.0 27.251806 6 GTACATG 15280 0.0 27.18738 1 TACATGG 15705 0.0 25.931213 2 ACATGGG 15600 0.0 25.436232 3 GAGTACT 14955 0.0 25.124376 12-13 ACGCAGA 26815 0.0 24.390944 7 CATGGGG 4940 0.0 24.241777 4 CGCAGAG 26945 0.0 24.185127 8 GTACTTT 15785 0.0 23.701548 14-15 GCAGAGT 27835 0.0 23.035843 9 CATGGGA 10255 0.0 22.799238 4 AGTACTT 15230 0.0 22.269144 12-13 ACTTTTT 17520 0.0 21.625013 16-17 ATGGGAT 3790 0.0 20.814201 5 >>END_MODULE