FastQCFastQC Report
Thu 26 May 2016
SRR938030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989615
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269231.353176368292358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211491.0629694689676144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150340.7556235754153442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68680.3451924115972186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.25502421322718216No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45510.2287377206142897No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32710.16440366603589135No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26740.13439786089268527No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26620.13379472913101279No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25820.1297738507198629No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.11952061077143065No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21690.10901606592230155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115300.042.6405071
GTATCAA186000.034.6559831
TATCAAC227600.028.2173182
ATCAACG229300.027.9852923
TCAACGC232850.027.7197324
CAACGCA235650.027.3903665
AACGCAG240600.026.8268476
GCAACGC1658.419556E-825.9081943
GTACATG156150.025.1883471
TACGGCC950.001227657324.997254
GAGTACT149900.024.77712612-13
ACGCAGA267100.024.1652577
TACATGG157700.024.127492
CGCAGAG268300.024.0571738
CATTCCG3000.023.7473859
ACATGGG155800.023.2309043
GCAGAGT277900.022.9355839
GTACTTT162100.022.85374814-15
AGTACTT152250.022.38259312-13
CATGGGA100050.021.456914
Produced by FastQC (version 0.11.5)