FastQCFastQC Report
Thu 26 May 2016
SRR938029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983994
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280471.4136635493857341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211891.067997181443089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149890.7554962363797472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66870.3370473902642851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50570.2548898837395677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.21275265953425265No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35080.1768150508519683No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.143145594190305No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26600.1340729861078209No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.13311532192133646No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.11436526521753596No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21770.10972815441982182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG804.2127795E-947.5179752
GGTATCA120350.040.960771
GTATCAA189550.033.7864571
GTACATG159500.028.0943361
TATCAAC234000.027.3939112
GTACCGT1405.790498E-727.1531316
TACATGG163450.027.0659162
ATCAACG238100.026.8822843
TCAACGC239900.026.7400054
CAACGCA242050.026.4632245
ACATGGG163650.026.1907833
AACGCAG246250.026.0118716
CATGGGG51000.025.1565784
GAGTACT148500.024.4876112-13
CATGGGA107250.023.7479134
ACGCAGA269850.023.6062327
CGCAGAG271550.023.4409438
ATGGGAG36100.022.7717745
GTACTTT159200.022.73612414-15
AGTACTT151950.022.49259612-13
Produced by FastQC (version 0.11.5)