FastQCFastQC Report
Thu 26 May 2016
SRR938029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983994
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268361.3526250583419104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209511.0560011774229157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150290.757512371509188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68260.34405345983909225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51440.25927497764610175No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44460.22409341963735777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.16013153265584473No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27340.13780283609728658No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26160.1318552374654359No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25220.12711731991124972No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.12696610977654166No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20550.10357894227502704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115100.038.7519721
GTATCAA183700.034.5203861
TAAGCGC650.005847309729.228496
TATCAAC228400.027.7436282
ATCAACG231250.027.2969363
TCAACGC233250.027.0621974
GTACATG159550.026.8840351
CAACGCA235950.026.7525225
AACGCAG240500.026.325396
TACATGG162050.025.970972
CATGGGG48800.025.3054034
ACATGGG162450.025.0863953
GAGTACT146550.023.6266112-13
ACGCAGA267450.023.6193967
CGCAGAG267900.023.5442628
AGTACTT148550.022.98878112-13
GCAGAGT278000.022.4838569
ATGGGGA25900.022.3727725
GTACTTT156000.022.10404614-15
ATGGGAG35400.021.6014215
Produced by FastQC (version 0.11.5)