##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938029_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983994 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.540119576974526 34.0 31.0 34.0 31.0 34.0 2 32.7379447720104 34.0 31.0 34.0 31.0 34.0 3 32.77953915183211 34.0 31.0 34.0 31.0 34.0 4 36.24122099159574 37.0 35.0 37.0 35.0 37.0 5 36.22060500182964 37.0 37.0 37.0 35.0 37.0 6 36.275296195452206 37.0 37.0 37.0 35.0 37.0 7 36.24178601346576 37.0 37.0 37.0 35.0 37.0 8 36.24658139087114 37.0 37.0 37.0 35.0 37.0 9 37.94337331665317 39.0 38.0 39.0 35.0 39.0 10-11 37.940671443562834 39.0 38.0 39.0 35.0 39.0 12-13 37.88587616696421 39.0 38.0 39.0 35.0 39.0 14-15 39.15775753354093 40.5 38.5 41.0 36.0 41.0 16-17 39.1282329986885 40.0 38.5 41.0 36.0 41.0 18-19 39.20875365550501 40.0 39.0 41.0 36.0 41.0 20-21 39.18186572136811 40.0 39.0 41.0 36.0 41.0 22-23 39.0296510473318 40.0 38.5 41.0 35.0 41.0 24-25 38.97042480975245 40.0 38.0 41.0 35.0 41.0 26-27 38.907494679923424 40.0 38.0 41.0 35.0 41.0 28-29 38.830314002965736 40.0 38.0 41.0 35.0 41.0 30-31 38.569452326972765 40.0 38.0 41.0 35.0 41.0 32-33 38.32577215455289 40.0 37.0 41.0 34.5 41.0 34-35 38.103128840107374 40.0 37.0 41.0 34.0 41.0 36-37 37.94719112053767 40.0 37.0 41.0 34.0 41.0 38-39 37.884631203521785 40.0 37.0 41.0 33.5 41.0 40-41 37.788629149080094 40.0 37.0 41.0 33.0 41.0 42-43 37.59331202614524 40.0 36.5 41.0 33.0 41.0 44-45 37.30290867815124 40.0 36.0 41.0 33.0 41.0 46-47 36.84891965399089 40.0 36.0 41.0 31.5 41.0 48-49 36.41411642373919 40.0 35.0 41.0 30.5 41.0 50-51 36.10420822845231 40.0 35.0 41.0 30.0 41.0 52-53 35.712832800905645 39.0 35.0 41.0 28.5 41.0 54-55 35.50716131197977 39.0 35.0 41.0 28.0 41.0 56-57 35.29545401851014 39.0 35.0 40.5 28.0 41.0 58-59 35.000638862819144 38.0 35.0 40.0 26.5 41.0 60-61 34.68071198804029 38.0 34.5 40.0 26.0 41.0 62-63 34.346339253042096 37.5 34.0 40.0 26.0 41.0 64-65 33.98773005361912 37.0 34.0 40.0 25.5 41.0 66-67 33.59663360877099 36.0 34.0 39.0 23.5 41.0 68-69 33.17003403236099 36.0 33.0 39.0 22.5 40.5 70-71 32.68999200602421 35.0 33.0 38.5 21.5 40.0 72-73 32.298585580399944 35.0 32.5 37.5 20.5 40.0 74-75 31.871351929491723 35.0 32.0 37.0 19.0 39.0 76-77 30.5293108245287 34.0 30.5 35.5 18.0 38.5 78-79 31.23709698718847 35.0 32.0 36.0 18.0 39.0 80-81 31.07937145979272 35.0 32.0 36.0 16.0 37.5 82-83 30.7322985855804 35.0 31.5 35.0 11.5 37.0 84-85 30.371209539948204 34.5 31.0 35.0 7.0 36.5 86-87 29.95681287342603 34.0 31.0 35.0 2.0 36.0 88-89 29.529534363511182 34.0 30.0 35.0 2.0 36.0 90-91 29.22081392383243 34.0 30.0 35.0 2.0 35.0 92-93 28.869660644135013 34.0 29.0 35.0 2.0 35.0 94-95 28.521006615947428 34.0 29.0 35.0 2.0 35.0 96-97 28.00993626996856 34.0 29.0 35.0 2.0 35.0 98-99 27.407840699115017 34.0 28.0 35.0 2.0 35.0 100-101 25.08273034091837 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04826274961821042 1101 2 0.0382373381004939 1101 3 0.12004972789952006 1101 4 0.041717066188020624 1101 5 0.009028504757829126 1101 6 0.10840968925351291 1101 7 0.07574949899623107 1101 8 0.003457508408786225 1101 9 -0.07242735491185925 1101 10-11 -0.010008471607754643 1101 12-13 0.022352404753235078 1101 14-15 0.0769728648155521 1101 16-17 0.11761219631251407 1101 18-19 0.10667975080794889 1101 20-21 0.027722357485316707 1101 22-23 0.11858655220526515 1101 24-25 0.10379940530685872 1101 26-27 0.0912249872417803 1101 28-29 0.11274073343242463 1101 30-31 0.09907779372734637 1101 32-33 0.021146460822933477 1101 34-35 -0.031785072761117306 1101 36-37 0.16090669867600838 1101 38-39 0.10849155354241447 1101 40-41 0.19765382781552177 1101 42-43 0.11115808156652207 1101 44-45 0.08654277718027004 1101 46-47 0.050032820313447246 1101 48-49 0.032731036292169335 1101 50-51 0.06702770801329905 1101 52-53 -0.13992535527957273 1101 54-55 0.11939411693795421 1101 56-57 -0.054570065931891065 1101 58-59 -0.15835022005762056 1101 60-61 -0.1998949109669539 1101 62-63 -0.02585435897452726 1101 64-65 0.11280079034071377 1101 66-67 -0.05424407112251117 1101 68-69 0.03608312719435247 1101 70-71 0.1250726308105925 1101 72-73 0.021554917416246155 1101 74-75 0.09303675815273493 1101 76-77 0.06328385617900167 1101 78-79 0.17415772795204632 1101 80-81 0.24062628421475551 1101 82-83 0.207136273254374 1101 84-85 0.19744757257449308 1101 86-87 0.1761273992151864 1101 88-89 0.21193080436649936 1101 90-91 0.08409885255297311 1101 92-93 0.11794208373425263 1101 94-95 0.1807309691502752 1101 96-97 0.18580729184982658 1101 98-99 0.1726999443157773 1101 100-101 0.5653936677170357 1102 1 0.11069989123726032 1102 2 0.10770823597179202 1102 3 0.04168443210821948 1102 4 0.06972645841423741 1102 5 0.03095579226543066 1102 6 0.06701234366594377 1102 7 0.044540837724383664 1102 8 0.09118882893026381 1102 9 0.09565553585144215 1102 10-11 0.15830519381265873 1102 12-13 0.11597083044681966 1102 14-15 0.10734089496742172 1102 16-17 0.2073383571560683 1102 18-19 0.11954149092276367 1102 20-21 0.0024814213531243468 1102 22-23 0.07893126974985165 1102 24-25 0.05574637752320655 1102 26-27 -0.06276738073461985 1102 28-29 -0.045450255270701234 1102 30-31 -0.05495169302577807 1102 32-33 -0.009491142345432024 1102 34-35 0.12565648616031666 1102 36-37 0.02477719613479934 1102 38-39 0.020042043476671267 1102 40-41 0.16462500281796366 1102 42-43 -0.03673135486631196 1102 44-45 -0.11576513734912908 1102 46-47 -0.04938889937763946 1102 48-49 -0.13897723363691483 1102 50-51 -0.05984779796096973 1102 52-53 -0.05535517727740569 1102 54-55 -0.14251210860312824 1102 56-57 -0.06249282528464306 1102 58-59 -0.19391118386933215 1102 60-61 -0.1512879568229195 1102 62-63 -0.2626718185721728 1102 64-65 -0.16770395783945702 1102 66-67 -0.2015139095130678 1102 68-69 0.023311580668242016 1102 70-71 0.022034769658333175 1102 72-73 -0.15372078607695272 1102 74-75 0.022282995047788745 1102 76-77 -0.07206228901084444 1102 78-79 -0.029363349130715477 1102 80-81 0.042072068966163556 1102 82-83 0.09764176045091588 1102 84-85 0.12322765473967578 1102 86-87 -0.11758928206384667 1102 88-89 -0.4510896563094313 1102 90-91 -0.25683291272199327 1102 92-93 0.0348302873534152 1102 94-95 -0.12796003130992872 1102 96-97 -0.13985118853096878 1102 98-99 -0.05206214280081767 1102 100-101 0.1361706090626491 1103 1 0.117609097644781 1103 2 0.09860119652815058 1103 3 0.1267563878607234 1103 4 0.07075585554723318 1103 5 0.05103158752893222 1103 6 0.07103043656425001 1103 7 0.15261924538605598 1103 8 0.15155461569836604 1103 9 0.1808729082993139 1103 10-11 0.09698654501439563 1103 12-13 0.18970474850834051 1103 14-15 0.21179217654771776 1103 16-17 0.18155144874051388 1103 18-19 0.26026020656168924 1103 20-21 0.21244272010279985 1103 22-23 0.21236990262882927 1103 24-25 0.24563283769816024 1103 26-27 0.16748209818888427 1103 28-29 0.25020293356435985 1103 30-31 0.16137573179761944 1103 32-33 0.3069049727327453 1103 34-35 0.24883526960934432 1103 36-37 0.2853489261087532 1103 38-39 0.2811192240255238 1103 40-41 0.2859758728945181 1103 42-43 0.20382931578528485 1103 44-45 0.16714336238427308 1103 46-47 0.22317205445383337 1103 48-49 0.3442832637902242 1103 50-51 0.2633414452854481 1103 52-53 0.36723849531052366 1103 54-55 0.46657847579341194 1103 56-57 0.611051158163356 1103 58-59 0.5256747229568361 1103 60-61 0.5524472113119501 1103 62-63 0.5000164258911539 1103 64-65 0.4407140743410025 1103 66-67 0.4246845026386694 1103 68-69 0.4396191701441481 1103 70-71 0.39875298271675064 1103 72-73 0.26646426304582604 1103 74-75 0.1703863914205357 1103 76-77 0.1563822370710426 1103 78-79 0.13617155470292275 1103 80-81 0.22617024309372624 1103 82-83 0.23243442685736326 1103 84-85 0.33409940231670276 1103 86-87 0.3748146089807811 1103 88-89 0.3994522759978203 1103 90-91 0.46129500039536 1103 92-93 0.48299720786150857 1103 94-95 0.2711938093490893 1103 96-97 0.204227992135241 1103 98-99 0.5493357070730305 1103 100-101 0.7092766203847383 1104 1 0.14718986120264077 1104 2 0.18605717481528927 1104 3 0.18317580587216042 1104 4 0.11303379570246364 1104 5 0.03729805108950046 1104 6 0.03214689912265811 1104 7 0.08393269884595611 1104 8 0.08627219879873849 1104 9 0.045133644173759535 1104 10-11 0.10005907487605725 1104 12-13 0.12202095969937687 1104 14-15 0.15226957411728392 1104 16-17 0.10708637076593419 1104 18-19 0.17336897954229613 1104 20-21 0.190003289381508 1104 22-23 0.21388391353005431 1104 24-25 0.18889788407496866 1104 26-27 0.1649934786932974 1104 28-29 0.1626759192357099 1104 30-31 0.19960034363428747 1104 32-33 0.27933237743538797 1104 34-35 0.3786502457754253 1104 36-37 0.48448726776793904 1104 38-39 0.34224352707648364 1104 40-41 0.3899484677202665 1104 42-43 0.366002535256591 1104 44-45 0.475201767569132 1104 46-47 0.35181051460467216 1104 48-49 0.45153972807000287 1104 50-51 0.5258647162210366 1104 52-53 0.488725158809558 1104 54-55 0.5030783266471985 1104 56-57 0.5991530633440973 1104 58-59 0.5816082367590525 1104 60-61 0.5607755239789967 1104 62-63 0.4800989220210141 1104 64-65 0.44638059873908276 1104 66-67 0.6181661942042993 1104 68-69 0.5900923703974073 1104 70-71 0.5672945390957906 1104 72-73 0.5112478300808618 1104 74-75 0.6098912436832578 1104 76-77 0.5043374012438839 1104 78-79 0.5302501848215009 1104 80-81 0.5806910828871139 1104 82-83 0.6126076781622452 1104 84-85 0.7083813782623061 1104 86-87 0.7880126928351423 1104 88-89 0.5287831164868315 1104 90-91 0.6980305671097007 1104 92-93 0.7533301694100807 1104 94-95 0.5690212560248966 1104 96-97 0.7335439180030896 1104 98-99 0.6902280317613823 1104 100-101 0.7008839036717589 1105 1 0.07894037736237891 1105 2 0.047483482142823163 1105 3 0.022391842779427407 1105 4 0.02682598736551256 1105 5 0.07022459911893719 1105 6 0.03317540226213822 1105 7 0.04589334803779366 1105 8 0.0021437495786642558 1105 9 0.03305902276813555 1105 10-11 0.05479185215074267 1105 12-13 -0.014672641228322902 1105 14-15 0.11484369967153896 1105 16-17 0.13896138132901825 1105 18-19 0.11482716964936657 1105 20-21 0.052272734851214864 1105 22-23 0.06640329643907705 1105 24-25 0.07654011763595747 1105 26-27 0.09821614730592643 1105 28-29 -0.0180490923359784 1105 30-31 0.09940170323201869 1105 32-33 0.0867137479260478 1105 34-35 0.13358545253100118 1105 36-37 0.21216564205149524 1105 38-39 0.09124023902854361 1105 40-41 0.16625680385678976 1105 42-43 0.16488764657644595 1105 44-45 0.21702907935687676 1105 46-47 0.24870396080711288 1105 48-49 0.17203643538793756 1105 50-51 0.08284823345643133 1105 52-53 0.05287071612850269 1105 54-55 -0.04942630884070809 1105 56-57 -0.10097625600108273 1105 58-59 0.03360429066537307 1105 60-61 -0.01800228281506122 1105 62-63 0.02649877939900591 1105 64-65 0.052541994951859294 1105 66-67 -0.04405911804030893 1105 68-69 -0.1388106395485167 1105 70-71 0.08116801876206381 1105 72-73 -0.0696439554692816 1105 74-75 -0.010572152454280115 1105 76-77 -0.03955839309692166 1105 78-79 0.038957296676738196 1105 80-81 0.040862005770367915 1105 82-83 0.12404933262117268 1105 84-85 0.18630931380326032 1105 86-87 0.15888907128286434 1105 88-89 -0.0037532109637119504 1105 90-91 0.13323418783328478 1105 92-93 -0.03656078690429254 1105 94-95 -0.22980824579576975 1105 96-97 0.22165629716828406 1105 98-99 0.3001898193123793 1105 100-101 0.5436112443001129 1106 1 -0.17515650232396496 1106 2 -0.08924118224729227 1106 3 -0.10478655635951384 1106 4 -0.1104404537114334 1106 5 -0.08456324587975672 1106 6 -0.09667780955215477 1106 7 -0.11744316007079192 1106 8 -0.18277787477057217 1106 9 -0.19190787439237766 1106 10-11 -0.07152884144731075 1106 12-13 -0.19673490295885898 1106 14-15 -0.23237308069338525 1106 16-17 -0.1812331575413353 1106 18-19 -0.14737106538208877 1106 20-21 -0.12174882780929863 1106 22-23 -0.144920455783911 1106 24-25 -0.026840243668956987 1106 26-27 -0.12691787725082548 1106 28-29 -0.09643984264118899 1106 30-31 -0.04509715978546325 1106 32-33 -0.08815171129356969 1106 34-35 0.016328613213339338 1106 36-37 -0.17125067422839635 1106 38-39 -0.13715335050657274 1106 40-41 -0.05958457611217938 1106 42-43 -0.15661913273021355 1106 44-45 0.0039021580371070286 1106 46-47 0.1588451737777561 1106 48-49 0.08207066101379468 1106 50-51 0.10522510767080462 1106 52-53 0.01987370248212983 1106 54-55 -0.183853003796429 1106 56-57 -0.1040059724136384 1106 58-59 0.07461709559220253 1106 60-61 0.10013742794485125 1106 62-63 0.10978165187370337 1106 64-65 -0.05971921936096436 1106 66-67 -0.09691306031102442 1106 68-69 -0.195651634930897 1106 70-71 -0.13326456114290153 1106 72-73 0.06579234382684973 1106 74-75 -0.053206415764464055 1106 76-77 -0.02748779435496118 1106 78-79 0.056224331410842154 1106 80-81 -0.20938216800082898 1106 82-83 -0.2325354867909013 1106 84-85 -0.09123160337123792 1106 86-87 0.1481549754872482 1106 88-89 0.07721865743024026 1106 90-91 -0.21383366201791176 1106 92-93 -2.1446792946022697E-5 1106 94-95 -0.21401322270766698 1106 96-97 0.012906564959287437 1106 98-99 -0.04155563598394352 1106 100-101 -0.05317474745579531 1107 1 0.00855473848660182 1107 2 0.05510742233580146 1107 3 0.01788125153116482 1107 4 0.05190358105654269 1107 5 0.05470838757089069 1107 6 0.036170503576101964 1107 7 0.03180065604457383 1107 8 0.014797762064404196 1107 9 -0.11888109744018749 1107 10-11 0.02641646342429027 1107 12-13 0.05916441353650015 1107 14-15 0.0891622345328571 1107 16-17 0.0319460121690085 1107 18-19 0.11523724636882804 1107 20-21 0.05655820080246343 1107 22-23 0.10617846423448185 1107 24-25 0.10441699658575487 1107 26-27 0.04503629670221443 1107 28-29 -0.013831088174065087 1107 30-31 0.10498966178268176 1107 32-33 0.0789116782893089 1107 34-35 0.13735320609890067 1107 36-37 -0.018234055153719453 1107 38-39 0.013047224532598989 1107 40-41 0.01322440905928346 1107 42-43 0.12077319499784522 1107 44-45 0.06817274618558855 1107 46-47 0.14062764762675073 1107 48-49 0.07108428929451094 1107 50-51 0.19301560944043672 1107 52-53 0.20170708869545706 1107 54-55 0.2260805641764847 1107 56-57 0.1306673153199256 1107 58-59 0.2133103695031906 1107 60-61 0.12097152988271631 1107 62-63 0.07273663501359806 1107 64-65 0.147800315491871 1107 66-67 0.09473226989378247 1107 68-69 0.18667783939279303 1107 70-71 0.2551072516078179 1107 72-73 0.16730458861939468 1107 74-75 0.16109713014644456 1107 76-77 0.15250708981326255 1107 78-79 0.07110574495451516 1107 80-81 0.20743981827005342 1107 82-83 0.130218675356371 1107 84-85 0.3705573144581713 1107 86-87 0.32162914176653956 1107 88-89 0.30642169446345235 1107 90-91 0.32486735942677214 1107 92-93 0.2838005960472252 1107 94-95 0.2408628044554284 1107 96-97 0.47995384459114376 1107 98-99 0.5391672703987922 1107 100-101 0.7251807339857841 1108 1 0.03578530361669863 1108 2 -0.00639583785683584 1108 3 -0.0069775098185616 1108 4 0.023647787675017184 1108 5 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96-97 -0.32139658421800377 2316 98-99 -0.38879662492974276 2316 100-101 -0.41489850128481365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 3.0 4 12.0 5 45.0 6 93.0 7 226.0 8 465.0 9 831.0 10 1556.0 11 2530.0 12 4155.0 13 6347.0 14 8210.0 15 11157.0 16 15794.0 17 24383.0 18 24152.0 19 14106.0 20 10534.0 21 10589.0 22 10377.0 23 10954.0 24 12293.0 25 14096.0 26 16680.0 27 19590.0 28 24227.0 29 29268.0 30 36005.0 31 44816.0 32 56561.0 33 74054.0 34 106900.0 35 181075.0 36 269378.0 37 444567.0 38 445371.0 39 52585.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.511576143879466 19.568355549462346 15.033815626458546 20.88625268019964 2 15.551007342395593 21.243947501745488 40.437619884205404 22.76742527165351 3 18.68269407193682 27.138365160304296 30.642961269288232 23.535979498470653 4 12.140762522467305 17.842140651635034 37.19053585847538 32.82656096742228 5 12.565417317500616 39.047819221136365 33.35520841051354 15.031555050849473 6 29.738850016683514 37.191392715905394 17.611141969179343 15.458615298231749 7 27.200939115743296 35.55272848607405 20.16422428696861 17.082108111214044 8 26.792336046217997 34.31737776771948 19.49219476519275 19.398091420869772 9 27.793355292504728 16.62865394647641 20.36577897514346 35.2122117858754 10-11 24.94916819304897 27.62682246014857 26.12820401674602 21.295805330056442 12-13 26.33687400264315 25.68752728082847 27.37699811592172 20.598600600606655 14-15 23.120004395174583 26.19118303785193 27.264774994279218 23.424037572694274 16-17 20.60206513345847 29.992772133702424 27.675822636024005 21.729340096815104 18-19 20.408781478169793 27.12281387947746 32.049189664888104 20.41921497746465 20-21 21.734743149424848 26.08951942394987 32.15753172640643 20.018205700218854 22-23 21.19887963370857 26.356606925222557 31.932757861162887 20.511755579905987 24-25 20.77172006371353 26.736143315119865 32.35760227433111 20.134534346835494 26-27 20.541683849455577 26.937361550413545 32.27744465293455 20.243509947196323 28-29 20.063493135563917 26.928483654688474 32.739413526452196 20.268609683295413 30-31 20.295827507542867 26.848342283293196 32.55382828778716 20.302001921376778 32-33 20.143594799457958 27.100500482488403 32.229519822599514 20.526384895454125 34-35 20.719191980298596 27.039848962626316 31.960133392820683 20.280825664254404 36-37 21.03629630413711 26.715101054931118 31.85033577445876 20.398266866473012 38-39 20.547393085988197 26.89013144280069 32.02748383841108 20.53499163280003 40-41 20.410562418034843 26.89314150598366 32.24837909687157 20.44791697910993 42-43 20.597869070368464 27.03509223829272 31.64844348983708 20.718595201501742 44-45 20.321139715333274 27.40920312873311 31.41863590220239 20.851021253731222 46-47 20.62198776810817 27.4705719876169 30.902059179614454 21.005381064660476 48-49 20.760067822785754 27.432441831981347 30.824513582198332 20.982976763034568 50-51 20.872820179899737 27.777125334048392 30.309617871828245 21.04043661422363 52-53 20.91324368924503 28.01069458879412 30.252258827395647 20.823802894565205 54-55 20.47866576209404 27.84930297168237 30.226401894360567 21.44562937186302 56-57 20.693560565203324 28.283704487009537 29.851602373797505 21.17113257398964 58-59 20.722567709378154 27.680174436011402 30.223780918692295 21.373476935918152 60-61 20.7962322466701 27.877428056738076 30.313650142087123 21.0126895545047 62-63 20.726751189771743 27.954167200102418 30.241774924722552 21.077306685403283 64-65 20.483428881337343 27.689473859295944 30.39830261583452 21.428794643532186 66-67 20.445273524012674 28.0809064946769 29.943034101917647 21.53078587939278 68-69 20.386740763123615 28.10330997048625 30.025219374790353 21.484729891599784 70-71 20.464300799296772 28.12397618137958 29.84910740657482 21.56261561274883 72-73 20.50230494648673 28.071304651122936 29.626576491662775 21.799813910727554 74-75 20.331185477375435 28.40434497281746 29.33504335194562 21.929426197861485 76-77 20.294441414641376 28.10812431892435 29.76941966558367 21.828014600850608 78-79 20.300641030164407 28.031284368803533 29.781415669603838 21.886658931428222 80-81 20.125665702618054 28.730580838450116 29.477458097151505 21.666295361780328 82-83 20.359940604659084 28.46936533074193 29.26672157274669 21.903972491852294 84-85 20.211523800522947 28.134152411555302 29.224988186371796 22.429335601549948 86-87 20.276115720516312 28.019437627363487 29.41702743011925 22.287419222000953 88-89 20.30852442953399 27.868408743583643 29.476731761015166 22.3463350658672 90-91 20.109355417455586 28.027618089712675 29.656961390168647 22.206065102663093 92-93 20.082777697552178 27.77455615292125 29.615065813133494 22.52760033639308 94-95 19.876345392173565 27.662608858696146 29.66178325136064 22.79926249776965 96-97 20.101603155453887 27.556491490521516 29.389839230824094 22.952066123200503 98-99 19.887005706670482 27.54481616375856 29.361933554234536 23.206244575336417 100-101 19.85016083717995 27.42425128301799 29.685523242509802 23.04006463729225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7225.0 1 4515.5 2 2788.5 3 3721.5 4 2853.0 5 2484.5 6 5060.0 7 10795.0 8 16226.5 9 16821.0 10 11890.0 11 7275.5 12 6279.5 13 6164.5 14 6145.0 15 5924.5 16 5624.5 17 5545.5 18 5600.5 19 5780.5 20 6038.5 21 6613.5 22 7334.5 23 8405.0 24 9601.5 25 11103.0 26 13715.0 27 16425.5 28 18312.5 29 20467.0 30 24012.5 31 27789.0 32 32157.0 33 36795.5 34 41575.0 35 45111.5 36 49344.0 37 54226.5 38 55694.5 39 55431.5 40 55051.0 41 57632.0 42 60579.5 43 63230.5 44 65200.0 45 66556.0 46 65830.0 47 64523.5 48 73156.0 49 74210.5 50 65833.0 51 61218.0 52 58149.0 53 57963.0 54 57502.5 55 53729.5 56 47338.5 57 42130.0 58 38388.5 59 33743.5 60 28936.5 61 24877.0 62 21162.5 63 16605.5 64 12755.0 65 10120.0 66 7398.5 67 6388.5 68 4879.5 69 2855.5 70 2236.0 71 1930.0 72 1668.5 73 1435.5 74 1147.5 75 992.0 76 692.0 77 341.5 78 164.0 79 80.5 80 34.5 81 18.0 82 19.0 83 12.5 84 4.5 85 5.5 86 3.5 87 0.5 88 1.5 89 1.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015574643874931073 3 0.007157279709515251 4 0.0 5 5.040337823602289E-5 6 0.0 7 0.0 8 2.5201689118011444E-4 9 4.53630404124206E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.024202694161373E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00574598511890661 26-27 0.021169418859129617 28-29 0.0 30-31 0.0 32-33 4.284287150061946E-4 34-35 0.019102880351452675 36-37 0.003679446611229671 38-39 0.017918400962906138 40-41 0.01514621515992488 42-43 0.008316557408943777 44-45 0.0030242026941613735 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7641182382608012E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002847790870335293 86-87 3.5282364765216025E-4 88-89 1.0080675647204578E-4 90-91 2.26815202062103E-4 92-93 2.7721858029812593E-4 94-95 0.0 96-97 2.5201689118011445E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983994.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.84296911156316 #Duplication Level Percentage of deduplicated Percentage of total 1 70.23768738462456 29.38953383702475 2 14.942832825243382 12.505049846918217 3 5.800352775025596 7.28111946004697 4 2.9031964291000247 4.859134340305311 5 1.5775283128027502 3.3004234232610905 6 0.997598835792928 2.504549836308891 7 0.6426812174508235 1.8824183231263627 8 0.4605194887730017 1.5415602195201252 9 0.3417619095064133 1.2870299720687122 >10 1.7905391240138018 13.915037708327263 >50 0.18126508834248378 5.321236319124048 >100 0.11489403245815548 8.909223278825479 >500 0.006496041457410192 1.8095742871651943 >1k 0.0020450500884439497 1.7296912720405444 >5k 2.4059412805222936E-4 0.6025202842104973 >10k+ 3.6089119207834407E-4 3.161897591726533 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26836 1.3526250583419104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20951 1.0560011774229157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15029 0.757512371509188 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6826 0.34405345983909225 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5144 0.25927497764610175 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4446 0.22409341963735777 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3177 0.16013153265584473 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2734 0.13780283609728658 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2616 0.1318552374654359 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2522 0.12711731991124972 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2519 0.12696610977654166 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2055 0.10357894227502704 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.5201689118011445E-5 0.0 18-19 0.0 0.0 0.0 5.040337823602289E-5 0.0 20-21 0.0 0.0 0.0 5.040337823602289E-5 0.0 22-23 0.0 0.0 0.0 5.796388497142633E-4 0.0 24-25 0.0 0.0 0.0 0.0017137148600247783 0.0 26-27 0.0 0.0 0.0 0.004208682082707912 0.0 28-29 0.0 0.0 0.0 0.01010587733632259 0.0 30-31 0.0 0.0 0.0 0.030569648900147885 0.0 32-33 0.0 0.0 0.0 0.06396188698151305 0.0 34-35 0.0 0.0 0.0 0.11290356724869127 0.0 36-37 0.0 0.0 0.0 0.18923948358714793 0.0 38-39 0.0 0.0 0.0 0.30531846366470866 0.0 40-41 0.0 0.0 0.0 0.42847911838443065 0.0 42-43 0.0 0.0 0.0 0.5471538724411464 0.0 44-45 0.0 0.0 0.0 0.6836210190151786 0.0 46-47 0.0 0.0 0.0 0.8397454831012594 0.0 48-49 0.0 0.0 0.0 1.005975824523663 0.0 50-51 0.0 0.0 0.0 1.1816063959870846 0.0 52-53 0.0 0.0 0.0 1.3446613245806187 0.0 54-55 0.0 0.0 0.0 1.5176961220648852 0.0 56-57 0.0 0.0 0.0 1.7224094427704921 0.0 58-59 0.0 0.0 0.0 1.9391187674962727 0.0 60-61 0.0 0.0 0.0 2.1721083833922883 0.0 62-63 0.0 0.0 0.0 2.3952945422213983 0.0 64-65 0.0 0.0 0.0 2.616892994636072 0.0 66-67 0.0 0.0 0.0 2.8571406969980755 0.0 68-69 0.0 0.0 0.0 3.119616289162165 0.0 70-71 0.0 0.0 0.0 3.414602060288489 0.0 72-73 0.0 0.0 0.0 3.708277343580676 0.0 74-75 0.0 0.0 0.0 4.000112903567249 0.0 76-77 0.0 0.0 0.0 4.304650114869299 0.0 78-79 0.0 0.0 0.0 4.635850713258206 0.0 80-81 0.0 0.0 0.0 4.987187461252403 0.0 82-83 0.0 0.0 0.0 5.342959706531371 0.0 84-85 0.0 0.0 0.0 5.708459803809891 0.0 86-87 0.0 0.0 0.0 6.078874230466423 0.0 88-89 0.0 0.0 0.0 6.471844168883575 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11510 0.0 38.751972 1 GTATCAA 18370 0.0 34.520386 1 TAAGCGC 65 0.0058473097 29.22849 6 TATCAAC 22840 0.0 27.743628 2 ATCAACG 23125 0.0 27.296936 3 TCAACGC 23325 0.0 27.062197 4 GTACATG 15955 0.0 26.884035 1 CAACGCA 23595 0.0 26.752522 5 AACGCAG 24050 0.0 26.32539 6 TACATGG 16205 0.0 25.97097 2 CATGGGG 4880 0.0 25.305403 4 ACATGGG 16245 0.0 25.086395 3 GAGTACT 14655 0.0 23.62661 12-13 ACGCAGA 26745 0.0 23.619396 7 CGCAGAG 26790 0.0 23.544262 8 AGTACTT 14855 0.0 22.988781 12-13 GCAGAGT 27800 0.0 22.483856 9 ATGGGGA 2590 0.0 22.372772 5 GTACTTT 15600 0.0 22.104046 14-15 ATGGGAG 3540 0.0 21.601421 5 >>END_MODULE