FastQCFastQC Report
Thu 26 May 2016
SRR938028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052055
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275961.3447982632044462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211161.0290172534361894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149160.7268811021147094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73650.3589085087875325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.2452175989434981No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43660.21276232849509394No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.17182775315476437No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.1432710136911535No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28250.13766687540051314No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26930.1312342992756042No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.11115686470391875No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21650.10550399477596847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121200.038.859451
GTATCAA194250.035.6456341
TATCAAC241150.028.6780242
ATCAACG241200.028.6523573
TCAACGC242850.028.477274
CAACGCA245650.028.1333145
GTATAGG6000.027.7792031
AACGCAG251300.027.5319186
GTACATG162000.027.2206761
TACATGG165150.026.236862
GAGTACT150300.024.8597612-13
ACATGGG167500.024.818113
ACGCAGA277900.024.754297
CGCAGAG280050.024.5963978
GCAGAGT285950.023.6732869
AGTACTT152700.023.56623512-13
CATGGGG53550.023.4485784
GTACTTT163200.022.818314-15
GTGTACG2205.4942575E-821.6461331
CATGGGA106850.021.6338484
Produced by FastQC (version 0.11.5)