##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2052055 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.132875580820205 33.0 31.0 34.0 31.0 34.0 2 32.21716328266055 34.0 31.0 34.0 30.0 34.0 3 32.217991233178445 34.0 31.0 34.0 30.0 34.0 4 35.74579287592194 37.0 35.0 37.0 35.0 37.0 5 35.77618484884665 37.0 35.0 37.0 35.0 37.0 6 35.83319306743728 37.0 36.0 37.0 35.0 37.0 7 35.78114329294293 37.0 36.0 37.0 35.0 37.0 8 35.783369354135246 37.0 36.0 37.0 35.0 37.0 9 37.42902163928355 39.0 38.0 39.0 35.0 39.0 10-11 37.35386429700958 39.0 37.0 39.0 35.0 39.0 12-13 37.2118817478089 39.0 37.0 39.0 34.0 39.0 14-15 38.38306453774388 40.0 38.0 41.0 34.0 41.0 16-17 38.25225858956022 40.0 38.0 41.0 33.5 41.0 18-19 38.47320612751608 40.0 38.0 41.0 34.0 41.0 20-21 38.509757048422195 40.0 38.0 41.0 34.0 41.0 22-23 38.39539461661603 40.0 38.0 41.0 34.0 41.0 24-25 38.27796940140493 40.0 38.0 41.0 34.0 41.0 26-27 38.10607488590705 40.0 38.0 41.0 34.0 41.0 28-29 37.92981206644072 40.0 38.0 41.0 33.0 41.0 30-31 37.66008683977769 40.0 37.0 41.0 33.0 41.0 32-33 37.32681531440434 40.0 36.0 41.0 32.5 41.0 34-35 37.08345463450054 40.0 35.5 41.0 32.0 41.0 36-37 36.82485654624267 39.0 35.0 41.0 31.0 41.0 38-39 36.552102648320826 39.0 35.0 41.0 30.5 41.0 40-41 36.40314976937753 39.0 35.0 41.0 30.0 41.0 42-43 36.163094556432455 39.0 35.0 41.0 30.0 41.0 44-45 35.77493829356426 39.0 35.0 41.0 28.5 41.0 46-47 35.2743615546367 39.0 35.0 40.0 26.5 41.0 48-49 34.80222435558501 38.0 35.0 40.0 24.0 41.0 50-51 34.05213870973243 37.5 33.5 39.5 23.0 40.5 52-53 34.12254739760874 38.0 34.0 40.0 23.0 40.5 54-55 34.24247230215565 38.0 34.0 40.0 22.5 41.0 56-57 33.99361713014515 38.0 34.0 40.0 21.5 41.0 58-59 33.65443372619155 38.0 33.5 40.0 19.5 41.0 60-61 33.2237907853347 37.0 33.0 40.0 16.5 41.0 62-63 32.753823118776054 36.5 32.5 39.5 12.5 41.0 64-65 32.2912158787167 36.0 32.0 39.0 8.0 41.0 66-67 31.82023191386196 35.0 31.5 39.0 6.0 40.0 68-69 31.233619469263736 35.0 31.0 38.5 2.0 40.0 70-71 30.722413385606135 35.0 30.0 37.5 2.0 40.0 72-73 30.100984622731847 35.0 29.5 37.0 2.0 39.0 74-75 29.490539727249026 34.0 29.0 36.5 2.0 39.0 76-77 28.818080412074725 34.0 27.5 36.0 2.0 38.5 78-79 28.123545665199032 34.0 26.5 35.0 2.0 37.5 80-81 27.460837063334072 33.5 26.0 35.0 2.0 37.0 82-83 26.79177994741856 33.0 24.5 35.0 2.0 36.0 84-85 26.122251840228454 32.5 23.0 35.0 2.0 36.0 86-87 25.42816274417596 32.0 20.0 35.0 2.0 35.5 88-89 24.61145266574239 31.5 15.5 34.5 2.0 35.0 90-91 23.780949828342806 31.0 4.5 34.0 2.0 35.0 92-93 22.921570084622488 30.5 2.0 34.0 2.0 35.0 94-95 21.921135398417682 29.5 2.0 34.0 2.0 35.0 96-97 20.711724100962208 29.0 2.0 34.0 2.0 35.0 98-99 19.23692786012071 27.0 2.0 34.0 2.0 35.0 100-101 15.160990811649786 11.0 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08089390981283628 1101 2 0.1907445372542469 1101 3 0.16155869253402955 1101 4 0.11024071583093331 1101 5 0.06827273033641745 1101 6 0.0895110396070109 1101 7 0.18294472787599148 1101 8 0.265101901457534 1101 9 0.15146436397063923 1101 10-11 0.25829582049739486 1101 12-13 0.35202290022553484 1101 14-15 0.38353847251584483 1101 16-17 0.3841798360432662 1101 18-19 0.38462129812380397 1101 20-21 0.31646176493320155 1101 22-23 0.33073577869282644 1101 24-25 0.2641865812603825 1101 26-27 0.24548345033676355 1101 28-29 0.2041974374086024 1101 30-31 0.05489118340410215 1101 32-33 0.18656267671631355 1101 34-35 0.2453914266478705 1101 36-37 0.2474808029348381 1101 38-39 0.2721449177709445 1101 40-41 0.4536345661325072 1101 42-43 0.5592193565147667 1101 44-45 0.5416227687253325 1101 46-47 0.6019930242126534 1101 48-49 0.7862801205855234 1101 50-51 0.598250565825019 1101 52-53 0.6400393220292031 1101 54-55 0.44908650954828033 1101 56-57 0.4342197281872515 1101 58-59 0.5133514095409453 1101 60-61 0.5310768083755804 1101 62-63 0.4080217539249773 1101 64-65 0.24840436522835319 1101 66-67 0.28377252654003016 1101 68-69 0.4083714936152454 1101 70-71 0.41850104608344907 1101 72-73 0.4285097969887097 1101 74-75 0.3677216194201378 1101 76-77 -0.051746732117454286 1101 78-79 -0.14500071169356232 1101 80-81 -0.1588014326889322 1101 82-83 0.02779651702094199 1101 84-85 -0.17087685718050238 1101 86-87 -0.12304622479511806 1101 88-89 -0.27871963456460236 1101 90-91 -0.049968876843951904 1101 92-93 -0.16790932813247395 1101 94-95 -0.13742725523155386 1101 96-97 -0.08123725561553741 1101 98-99 -0.050670781218922656 1101 100-101 -0.6365229729843271 1102 1 0.2636627300631318 1102 2 0.277255760599175 1102 3 0.2541441652925869 1102 4 0.23261941914832107 1102 5 0.27134614072551955 1102 6 0.31348375853772126 1102 7 0.2434360386340373 1102 8 0.2611815413254135 1102 9 0.34539954621449453 1102 10-11 0.34713826793391434 1102 12-13 0.29328070344840995 1102 14-15 0.37593206999235207 1102 16-17 0.40304315102198274 1102 18-19 0.31737030534360855 1102 20-21 0.3607578063224608 1102 22-23 0.4299140782828772 1102 24-25 0.3246900090784024 1102 26-27 0.2946060114509592 1102 28-29 0.14897344536787216 1102 30-31 0.23511918717414204 1102 32-33 0.21530892779620814 1102 34-35 0.10870853403711322 1102 36-37 0.14254853730006545 1102 38-39 0.05206259381053968 1102 40-41 0.06063049807523413 1102 42-43 0.16367889306669525 1102 44-45 0.3315709142584282 1102 46-47 0.2421163431343274 1102 48-49 0.0913814683300771 1102 50-51 0.06435440051426866 1102 52-53 -0.044582753420122856 1102 54-55 0.07765441060495704 1102 56-57 -0.010713801551617053 1102 58-59 -0.050501894126334435 1102 60-61 -0.09473871888354068 1102 62-63 -0.22381726209115982 1102 64-65 -0.14760080384393603 1102 66-67 -0.1827816042967072 1102 68-69 -0.3290234891359596 1102 70-71 -0.3844123536164403 1102 72-73 -0.4093477299652015 1102 74-75 -0.41704664672027647 1102 76-77 -0.6378143728210794 1102 78-79 -0.5911901583238084 1102 80-81 -0.286960464843677 1102 82-83 -0.5247577919693249 1102 84-85 -0.3405369834730152 1102 86-87 -0.3979942688069009 1102 88-89 -0.3478666197418363 1102 90-91 -0.6143703956028013 1102 92-93 -0.7123887628618029 1102 94-95 -0.5180570214115932 1102 96-97 -0.6491723710518151 1102 98-99 -1.1333035629094539 1102 100-101 -1.1008460910902933 1103 1 0.2510762563384219 1103 2 0.25739196945274756 1103 3 0.26985178313213964 1103 4 0.12555503613317143 1103 5 0.163383826657153 1103 6 0.17816048483178548 1103 7 0.23850544611070745 1103 8 0.13294352413503674 1103 9 0.19956665031891418 1103 10-11 0.27208969690411067 1103 12-13 0.2530698810942482 1103 14-15 0.4359143583949603 1103 16-17 0.3531378846007769 1103 18-19 0.23248617797326432 1103 20-21 0.34103327477198064 1103 22-23 0.37640905937938385 1103 24-25 0.27361848213862316 1103 26-27 0.31569967171189006 1103 28-29 0.3596889617893879 1103 30-31 0.3517183752073265 1103 32-33 0.2628983472782309 1103 34-35 0.25315470078550817 1103 36-37 0.40031712305248845 1103 38-39 0.28381557260726 1103 40-41 0.6767841171573181 1103 42-43 0.6663646579569757 1103 44-45 0.7743589963542803 1103 46-47 0.7910894039676108 1103 48-49 0.7333515315310848 1103 50-51 0.6896330740959442 1103 52-53 0.7784031699358422 1103 54-55 0.7292701306974863 1103 56-57 0.7774779110511574 1103 58-59 0.8332268417670718 1103 60-61 0.9838716247387325 1103 62-63 0.8904419941946671 1103 64-65 0.6910065878454503 1103 66-67 0.5862531136454869 1103 68-69 0.6687285954350664 1103 70-71 0.4976255052317633 1103 72-73 0.7201494904009103 1103 74-75 0.578811692613673 1103 76-77 0.6737349720103829 1103 78-79 0.45988260373434287 1103 80-81 0.40425994326413317 1103 82-83 0.5260972804765416 1103 84-85 0.3894994895723798 1103 86-87 -0.03278952941069946 1103 88-89 0.05599794211844511 1103 90-91 -0.06162744473209969 1103 92-93 -0.26338680025871497 1103 94-95 -0.4617924392953654 1103 96-97 -0.60708385999936 1103 98-99 -0.9146888469681507 1103 100-101 -0.7870130123971979 1104 1 0.16010896080955206 1104 2 0.21280094042899833 1104 3 0.17882677889729592 1104 4 0.12780538855822954 1104 5 0.1312349094849381 1104 6 0.16769625992287018 1104 7 0.2126396001900801 1104 8 0.16238070634237545 1104 9 0.24457025352418782 1104 10-11 0.28472732929678557 1104 12-13 0.2951471361121776 1104 14-15 0.2683769410461778 1104 16-17 0.31879761569543064 1104 18-19 0.2633967983849175 1104 20-21 0.2933071700522092 1104 22-23 0.2709568786506509 1104 24-25 0.3531746706077925 1104 26-27 0.3101589181682627 1104 28-29 0.246343608201542 1104 30-31 0.3337557509254552 1104 32-33 0.35340727490117274 1104 34-35 0.342210431317433 1104 36-37 0.2800750048845728 1104 38-39 0.12795096125098837 1104 40-41 0.34630760402644256 1104 42-43 0.2815621451878414 1104 44-45 0.23182308632243576 1104 46-47 0.13566349361995123 1104 48-49 0.15412379460239833 1104 50-51 0.09731421770449344 1104 52-53 0.22211076767457882 1104 54-55 0.21019898246650115 1104 56-57 0.15266015284776557 1104 58-59 0.2218666963755851 1104 60-61 0.319034458035226 1104 62-63 0.3158343990399004 1104 64-65 0.15424915677289874 1104 66-67 0.3436564802737756 1104 68-69 0.38365175098156 1104 70-71 0.2499938590406785 1104 72-73 0.25597037521031396 1104 74-75 0.19696567437083345 1104 76-77 0.08624814308942064 1104 78-79 -0.009118633701096002 1104 80-81 -0.028353371081792034 1104 82-83 -0.2460241030394421 1104 84-85 -0.14150673779992218 1104 86-87 -0.10752933779482987 1104 88-89 -0.23439413255259822 1104 90-91 -0.46158796738348684 1104 92-93 -0.49499308332996605 1104 94-95 -0.555141439154685 1104 96-97 -0.893168309943519 1104 98-99 -0.9049794289647579 1104 100-101 -0.9772608153033051 1105 1 0.06830676348267417 1105 2 0.202064100600289 1105 3 0.18328171855731767 1105 4 0.15092841507321708 1105 5 0.1624389516147886 1105 6 0.17321201536211817 1105 7 0.12732124732014682 1105 8 0.12503723344129725 1105 9 0.2188103266242365 1105 10-11 0.17751829668291919 1105 12-13 0.2661194694743543 1105 14-15 0.291720294997468 1105 16-17 0.31284607151098953 1105 18-19 0.17467431903892106 1105 20-21 0.13489498743185635 1105 22-23 0.2815505732308594 1105 24-25 0.15067686036513095 1105 26-27 0.17584339305903995 1105 28-29 0.18265059596052424 1105 30-31 0.10526430449611013 1105 32-33 0.13970615244777917 1105 34-35 0.2393336038162417 1105 36-37 0.23447817435557994 1105 38-39 0.26243909871368487 1105 40-41 0.17447572332225292 1105 42-43 0.19279830375871398 1105 44-45 0.22558281857729412 1105 46-47 0.3694376620528459 1105 48-49 0.3587239070640038 1105 50-51 0.31443406501622917 1105 52-53 0.39092970447980946 1105 54-55 0.2757457189170438 1105 56-57 0.06612580880174335 1105 58-59 0.15978680537683942 1105 60-61 0.09287118323960186 1105 62-63 0.29630122286446436 1105 64-65 0.014874075670775255 1105 66-67 -0.059977030042414725 1105 68-69 0.11189394355044513 1105 70-71 0.02937553644887103 1105 72-73 -0.03637655804804396 1105 74-75 -0.311401631741024 1105 76-77 -0.3286088197982515 1105 78-79 -0.36492000378630607 1105 80-81 -0.18877512915956274 1105 82-83 -0.20537815165745954 1105 84-85 -0.02597879000806458 1105 86-87 0.16328430574511543 1105 88-89 -0.08313421105945196 1105 90-91 -0.14736022180625596 1105 92-93 -0.06117768798414858 1105 94-95 -0.6174239502927072 1105 96-97 -0.6005572541020037 1105 98-99 -0.7385624667259059 1105 100-101 -0.6207933264413281 1106 1 0.16580587104233047 1106 2 0.11920101467887889 1106 3 0.29348008058604336 1106 4 0.276774803206969 1106 5 0.2104450911466884 1106 6 0.16225244748630985 1106 7 0.1869488479828405 1106 8 0.12115139944435782 1106 9 0.24997222674414132 1106 10-11 0.2725787823992931 1106 12-13 0.31377192231231277 1106 14-15 0.35712587480909264 1106 16-17 -0.07814605899582006 1106 18-19 0.32125306889017935 1106 20-21 0.44800796809106913 1106 22-23 0.46502071881305795 1106 24-25 0.4295769104790921 1106 26-27 0.3658675404352678 1106 28-29 0.36968635535860983 1106 30-31 0.30202880779585684 1106 32-33 0.3665190077076872 1106 34-35 0.22240398189058652 1106 36-37 0.34028856167469 1106 38-39 0.4957238626975382 1106 40-41 0.4651106488606942 1106 42-43 0.3566037265923754 1106 44-45 0.548477746312507 1106 46-47 0.5912697276632244 1106 48-49 0.7137938835401911 1106 50-51 0.6820305286641641 1106 52-53 0.8141832673762579 1106 54-55 0.6858666789082548 1106 56-57 0.35133544075019785 1106 58-59 0.4544351381152154 1106 60-61 0.7045140400555567 1106 62-63 0.7613993684191982 1106 64-65 0.4343216093317963 1106 66-67 0.3428809815547318 1106 68-69 0.3114536157731216 1106 70-71 0.47267925906925967 1106 72-73 0.28606986120670186 1106 74-75 0.37412956476976333 1106 76-77 0.025415542678981495 1106 78-79 0.12559023753872012 1106 80-81 0.0672552790289167 1106 82-83 0.3135578878295924 1106 84-85 0.22868635607755294 1106 86-87 0.07549263221352476 1106 88-89 -0.17300996162466475 1106 90-91 -0.1956701343013343 1106 92-93 -0.1094199523570154 1106 94-95 -0.25710302073600033 1106 96-97 -0.4572301907341618 1106 98-99 -0.5700442723844361 1106 100-101 -0.8979828658963456 1107 1 -0.01120958069624578 1107 2 -0.05259416092173552 1107 3 -0.05153440469287318 1107 4 -0.051181823817813665 1107 5 0.0033501310634918013 1107 6 0.02123374633686126 1107 7 0.030232921300573423 1107 8 0.019477209415775576 1107 9 0.03111635081650377 1107 10-11 -0.021697344730419843 1107 12-13 0.0461799169781969 1107 14-15 -0.058210167399174395 1107 16-17 -0.11768572062651828 1107 18-19 -0.01596197845317704 1107 20-21 -0.07140408678959176 1107 22-23 0.15510854428654852 1107 24-25 0.08388985290736883 1107 26-27 0.05883654635250224 1107 28-29 -0.029115327582218242 1107 30-31 -0.07353757396655425 1107 32-33 0.07772413180250481 1107 34-35 -0.07946326774951018 1107 36-37 -0.08385945534057981 1107 38-39 -0.24029906284092561 1107 40-41 -0.1700516009768691 1107 42-43 -0.06476533167477072 1107 44-45 -0.0190590438178333 1107 46-47 -0.008631845234397417 1107 48-49 0.09773431307266378 1107 50-51 -0.0077606990106602325 1107 52-53 -0.16969590657951272 1107 54-55 -0.14460909338826866 1107 56-57 -0.32199112426783927 1107 58-59 -0.18869227270990763 1107 60-61 -0.0536298152976471 1107 62-63 -0.01912523022513568 1107 64-65 -0.020870052475473244 1107 66-67 0.10012110418979248 1107 68-69 0.02378937377410395 1107 70-71 0.014004315542873513 1107 72-73 -0.028958818948584764 1107 74-75 -0.32004480530839885 1107 76-77 -0.365168522662362 1107 78-79 -0.4609156467912392 1107 80-81 -0.44041018653282293 1107 82-83 -0.3902977286717757 1107 84-85 -0.4747522423700694 1107 86-87 -0.5237530181371639 1107 88-89 -0.40643823722984607 1107 90-91 -0.33305996525607995 1107 92-93 -0.42821650872492256 1107 94-95 -0.5594217495317757 1107 96-97 -0.5139810431624312 1107 98-99 -0.9389015809646324 1107 100-101 -1.0335059892957421 1108 1 -0.014469459665669149 1108 2 0.016634324541747958 1108 3 -0.036239412971816876 1108 4 0.09488202372560295 1108 5 0.06702042687498277 1108 6 0.082162612439582 1108 7 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-0.13629928097116206 2316 74-75 -0.20363316078839233 2316 76-77 -0.24352966981461677 2316 78-79 -0.08861313981914165 2316 80-81 -0.05028036385524004 2316 82-83 -0.3324013631102041 2316 84-85 -0.3102014392740102 2316 86-87 -0.23659584396151345 2316 88-89 -0.07079702803711996 2316 90-91 0.03696449300828064 2316 92-93 -0.123205920086086 2316 94-95 -0.13705996109575835 2316 96-97 -0.29047314642403066 2316 98-99 -0.22168348483172196 2316 100-101 -0.31405644937955834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6188.0 3 1490.0 4 1318.0 5 1471.0 6 1699.0 7 2193.0 8 2805.0 9 3382.0 10 4418.0 11 5939.0 12 8438.0 13 11189.0 14 13423.0 15 17276.0 16 22235.0 17 31280.0 18 28960.0 19 19347.0 20 18636.0 21 19380.0 22 20757.0 23 22922.0 24 25886.0 25 29244.0 26 33794.0 27 38677.0 28 45581.0 29 53633.0 30 64022.0 31 77405.0 32 96219.0 33 121982.0 34 163496.0 35 236270.0 36 278876.0 37 323656.0 38 186240.0 39 12328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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18-19 20.486917062102787 27.038681581419176 32.178977030142605 20.295424326335436 20-21 21.834818738304417 26.088655865052196 32.21059000627017 19.86593539037321 22-23 21.300358663735903 26.266772387677122 32.003951843558944 20.42891710502803 24-25 20.810334884116138 26.67780228666807 32.38941821472295 20.122444614492842 26-27 20.559006089934257 26.84890433400891 32.37691417628747 20.215175399769365 28-29 20.140416839774574 26.902835050100254 32.77234580141256 20.184402308712613 30-31 20.306448465928494 26.81935027559631 32.61960392175992 20.254597336715282 32-33 20.189816072987266 27.05857297384857 32.24441014987097 20.50720080329319 34-35 20.8308396924225 27.015532517321354 31.918506107896 20.235121682360145 36-37 21.002306201186485 26.705770721609056 31.8699836100474 20.421939467157056 38-39 20.58318700759041 26.893367354525637 32.009271238075456 20.51417439980849 40-41 20.46489958405954 26.94114689956017 32.21330985089676 20.380643665483525 42-43 20.62753297515219 27.028397603012838 31.610920388360874 20.7331490334741 44-45 20.375830929707693 27.384732611932822 31.460800690703657 20.77863576765583 46-47 20.583809927791673 27.536408891229197 30.88229701464157 20.99748416633756 48-49 20.83113949130166 27.49444714205761 30.694214497298162 20.98019886934257 50-51 21.02114306178605 27.8261630609913 30.16526679137271 20.987427085849944 52-53 20.976193621378215 28.025760549917155 30.165283213478467 20.83276261522616 54-55 20.528843001135762 27.869862340803596 30.215814535381625 21.385480122679017 56-57 20.798877325156017 28.24727313140589 29.83157927574994 21.122270267688148 58-59 20.855558917004522 27.660859756800654 30.176835239404916 21.306746086789904 60-61 20.97602460085282 27.784402267622998 30.281585776120735 20.957987355403446 62-63 20.87307020212658 27.79533025969872 30.348850702489333 20.982748835685367 64-65 20.688139699842292 27.565807274083426 30.413454202427292 21.332598823646986 66-67 20.6943915299034 27.9528934239799 30.04477484243248 21.30794020368422 68-69 20.702302412226217 27.977016107108625 30.062896792646367 21.25778468801879 70-71 20.723538741553465 28.11046280599262 29.840359943422417 21.325638509031496 72-73 20.843211396690915 28.04637036173884 29.588506188167386 21.521912053402854 74-75 20.716387960844518 28.41807287124118 29.414876216783583 21.450662951130724 76-77 20.843645183168917 28.170158134121188 29.52948232503243 21.456714357677463 78-79 20.911643930932243 28.092866983706944 29.54082452081433 21.454664564546487 80-81 20.836050822655917 28.856543860521388 29.128439025388907 21.17896629143379 82-83 21.125380677010423 28.510953359511177 28.929795555486233 21.43387040799217 84-85 21.066956384839706 28.21442654177201 28.862904912097537 21.855712161290747 86-87 21.252636396826038 28.090183659433343 29.048840180369268 21.60833976337135 88-89 21.367039783103216 27.93182477587413 29.090476875476963 21.61065856554569 90-91 21.204743809550987 28.172741559653865 29.163925641192627 21.45858898960252 92-93 21.373578394448643 27.85606800833669 29.049754578801917 21.72059901841275 94-95 21.28358245348809 27.893443222550047 28.921801467898856 21.90117285606301 96-97 21.632476119281442 27.623611885232112 28.67339310766548 22.070518887820967 98-99 21.500727340367256 27.62832919422584 28.57125095473327 22.299692510673633 100-101 22.35033474720377 26.797097963021425 28.771425797174572 22.081141492600235 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7447.0 1 4811.5 2 3085.0 3 3862.0 4 2929.5 5 2576.0 6 5065.5 7 10583.5 8 16079.0 9 16916.5 10 12066.0 11 7477.0 12 6477.0 13 6457.5 14 6444.0 15 6205.0 16 5989.0 17 5936.0 18 5984.0 19 6147.5 20 6493.5 21 7004.0 22 7881.5 23 9050.5 24 10282.0 25 12069.5 26 14841.5 27 17683.5 28 19556.0 29 21982.0 30 25378.0 31 28749.5 32 33249.5 33 38009.0 34 42707.0 35 46315.0 36 50558.0 37 55372.0 38 56921.5 39 56989.5 40 56720.0 41 58608.0 42 61704.5 43 64438.5 44 66450.0 45 68077.0 46 67479.0 47 66978.0 48 74139.0 49 74628.0 50 67034.5 51 62500.5 52 60102.5 53 59530.0 54 58713.5 55 55084.0 56 49074.5 57 44166.5 58 39978.0 59 35380.5 60 30601.5 61 26383.0 62 22606.5 63 18059.5 64 14072.0 65 11279.0 66 8749.5 67 7269.0 68 5597.5 69 3594.0 70 2707.5 71 2247.5 72 1924.0 73 1653.5 74 1372.0 75 1196.0 76 854.5 77 480.0 78 267.0 79 170.0 80 90.5 81 45.5 82 32.0 83 23.0 84 13.5 85 14.5 86 13.0 87 9.5 88 6.5 89 8.5 90 7.5 91 3.5 92 2.5 93 1.5 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.142978623867294 2 0.05019358642921365 3 0.14916754180565336 4 0.12602001408344318 5 0.18800665674165654 6 0.18927367931171435 7 0.1601808918376944 8 0.06954004644125035 9 0.11914885322274502 10-11 0.09870593137123519 12-13 0.147047715582672 14-15 0.1341581975141992 16-17 0.13130739673156908 18-19 0.1281642061250795 20-21 0.13011347161747613 22-23 0.18990719059674327 24-25 0.20818155458796184 26-27 0.14307608714191383 28-29 0.12253570201578418 30-31 0.09527035094088608 32-33 0.12143924017631108 34-35 0.08966621265024573 36-37 0.08350166053054134 38-39 0.04778137038237279 40-41 0.0565530650981577 42-43 0.05996427970985183 44-45 0.039643186951616796 46-47 0.05092456098886238 48-49 0.041860476449217976 50-51 0.0535317035849429 52-53 0.026607473971214223 54-55 0.04929205113898019 56-57 0.025632841225015898 58-59 0.049779367512079356 60-61 0.03625633815857762 62-63 0.05180173046044087 64-65 0.03898530984793293 66-67 0.038571090930798635 68-69 0.06405773724388479 70-71 0.05194792537237062 72-73 0.04890219804050087 74-75 0.0413244284388089 76-77 0.020004337115720583 78-79 0.023171893540865133 80-81 0.025779036136945647 82-83 0.021783041877532522 84-85 0.01978504474782596 86-87 0.023683575732619252 88-89 0.06383844487599016 90-91 0.10640553006620193 92-93 0.0779949855145208 94-95 0.07334111415142382 96-97 0.06539785726990749 98-99 0.05309311884915365 100-101 0.12660479373116218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2052055.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.458457835646556 #Duplication Level Percentage of deduplicated Percentage of total 1 72.14413721910775 31.352729454256934 2 13.962190128494226 12.135505019848939 3 5.512889860398213 7.1874507465203745 4 2.634440208406834 4.579548348703214 5 1.4954889061192351 3.2495820785129985 6 0.9450655532225679 2.4642654897986973 7 0.6108036292946031 1.858120863869158 8 0.43600777810296704 1.5158580512561386 9 0.3204115393720781 1.2532132236470457 >10 1.6553849970490415 13.465189446695044 >50 0.16669919369227648 5.093200267709498 >100 0.10850698651935586 8.782702383153037 >500 0.005390873388746151 1.5786990986918152 >1k 0.0020215775207798065 1.7732971294998001 >5k 2.246197245310896E-4 0.6050744389080982 >10k+ 3.369295867966344E-4 3.105563958929134 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27596 1.3447982632044462 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21116 1.0290172534361894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14916 0.7268811021147094 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7365 0.3589085087875325 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.2452175989434981 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4366 0.21276232849509394 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3526 0.17182775315476437 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2940 0.1432710136911535 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2825 0.13766687540051314 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2693 0.1312342992756042 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 0.11115686470391875 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2165 0.10550399477596847 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.218290932747904E-4 0.0 20-21 0.0 0.0 0.0 1.9492654923966462E-4 0.0 22-23 0.0 0.0 0.0 8.284378342685747E-4 0.0 24-25 0.0 0.0 0.0 0.0022660211349111014 0.0 26-27 0.0 0.0 0.0 0.005092456098886238 0.0 28-29 0.0 0.0 0.0 0.012012348596894332 0.0 30-31 0.0 0.0 0.0 0.03199231989395995 0.0 32-33 0.0 0.0 0.0 0.0677126100421285 0.0 34-35 0.0 0.0 0.0 0.11622495498415003 0.0 36-37 0.0 0.0 0.0 0.19519457324486916 0.0 38-39 0.0 0.0 0.0 0.3084225325344594 0.0 40-41 0.0 0.0 0.0 0.43037345490252454 0.0 42-43 0.0 0.0 0.0 0.5472562869903584 0.0 44-45 0.0 0.0 0.0 0.6832662867223345 0.0 46-47 0.0 0.0 0.0 0.8414004497930123 0.0 48-49 0.0 0.0 0.0 1.0086474290406446 0.0 50-51 0.0 0.0 0.0 1.1855676382942952 0.0 52-53 0.0 0.0 0.0 1.3487942574638594 0.0 54-55 0.0 0.0 0.0 1.525129687069791 0.0 56-57 0.0 0.0 0.0 1.7207384792317946 0.0 58-59 0.0 0.0 0.0 1.9356449997685248 0.0 60-61 0.0 0.0 0.0 2.1712137345246596 0.0 62-63 0.0 0.0 0.0 2.395769119248753 0.0 64-65 0.0 0.0 0.0 2.6167183628119126 0.0 66-67 0.0 0.0 0.0 2.8564049209207356 0.0 68-69 0.0 0.0 0.0 3.122406563176913 0.0 70-71 0.0 0.0 0.0 3.4150400452229595 0.0 72-73 0.0 0.0 0.0 3.7029221926312887 0.0 74-75 0.0 0.0 0.0 3.9840306424535408 0.0 76-77 0.0 0.0 0.0 4.281269264225374 0.0 78-79 0.0 0.0 0.0 4.603945800672983 0.0 80-81 0.0 0.0 0.0 4.95495978421631 0.0 82-83 0.0 0.0 0.0 5.307752472521448 0.0 84-85 0.0 0.0 0.0 5.669268123905061 0.0 86-87 0.0 0.0 0.0 6.029005070526862 0.0 88-89 0.0 0.0 0.0 6.405335139652689 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12120 0.0 38.85945 1 GTATCAA 19425 0.0 35.645634 1 TATCAAC 24115 0.0 28.678024 2 ATCAACG 24120 0.0 28.652357 3 TCAACGC 24285 0.0 28.47727 4 CAACGCA 24565 0.0 28.133314 5 GTATAGG 600 0.0 27.779203 1 AACGCAG 25130 0.0 27.531918 6 GTACATG 16200 0.0 27.220676 1 TACATGG 16515 0.0 26.23686 2 GAGTACT 15030 0.0 24.85976 12-13 ACATGGG 16750 0.0 24.81811 3 ACGCAGA 27790 0.0 24.75429 7 CGCAGAG 28005 0.0 24.596397 8 GCAGAGT 28595 0.0 23.673286 9 AGTACTT 15270 0.0 23.566235 12-13 CATGGGG 5355 0.0 23.448578 4 GTACTTT 16320 0.0 22.8183 14-15 GTGTACG 220 5.4942575E-8 21.646133 1 CATGGGA 10685 0.0 21.633848 4 >>END_MODULE