FastQCFastQC Report
Thu 26 May 2016
SRR938028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052055
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268641.3091267046935877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214841.0469504959662388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151990.7406721554734157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69780.34004936514859496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.24926232484022115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45970.22401933671368457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.15886513763032667No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28690.13981106744214944No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.13547395172156693No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.12567889262227377No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.1125213505485964No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21130.10296994963585283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113850.043.3486481
GTATCAA183650.035.356611
ATCAACG229900.028.0736873
TATCAAC230500.027.9222772
TCAACGC231850.027.8573764
CAACGCA235650.027.4484655
AACGCAG240200.026.9674076
GTACATG167900.026.2819791
CATTCCG2550.026.072689
TACATGG170450.025.5258922
ACATGGG170250.024.4642263
GAGTACT149250.024.40678412-13
ACGCAGA268000.024.116887
CGCAGAG268800.023.9744368
CATGGGG52550.023.2256984
GCAGAGT278800.022.8590169
GTACTTT158900.022.80500614-15
AGTACTT151950.022.2384112-13
CATGGGA111200.021.9516284
ATGGGGA26150.020.8850025
Produced by FastQC (version 0.11.5)