FastQCFastQC Report
Thu 26 May 2016
SRR938027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959101
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143920.734622666212717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113630.5800109335863746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73260.3739470297856006No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33940.17324272714883002No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33530.17114993050383823No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31700.16180891133228964No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30650.15644931016828637No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26850.13705265833665545No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26380.13465359876800634No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24440.12475109756975265No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23850.12173951215378892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.11719661211953851No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22780.11627782334856653No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA20250.10336373673434907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53500.057.6302951
GTATCAA100150.044.749361
TATCAAC127650.034.919192
ATCAACG129200.034.463493
GTGGTAT22150.034.045171
TCAACGC130800.034.005594
CAACGCA133350.033.3196755
AACGCAG136400.032.5687986
TGGTATC22100.032.038792
TAAGGTA6750.030.9764064
GTGTAAG6750.028.9902671
GTACGCT1004.940224E-528.5123753
ACGCAGA155500.028.3799867
CGCAGAG155800.028.2643598
TAAGGTG13050.026.9465625
GCAGAGT166400.025.8358129
CCGTCGT950.001226315525.0019159
AGGTAAG11100.024.8305972
GAGTACT104000.024.02465212-13
GTACATG93650.023.392521
Produced by FastQC (version 0.11.5)