FastQCFastQC Report
Thu 26 May 2016
SRR938027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959101
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136630.6974117209883512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112920.5763868223230961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75230.384002662445683No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34420.17569283053808865No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34140.1742636035610211No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32400.16538197877495853No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31530.1609411663819272No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28950.14777186066466202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26650.13603178192446433No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24910.12715015713840175No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24240.12373022115756155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.12214786271866536No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21970.11214327387919255No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA21550.10999943341359122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57600.053.5037961
GTATCAA99150.040.963521
TATCAAC129100.031.3866862
ATCAACG131450.030.7862423
TCAACGC133100.030.403044
CAACGCA135500.029.8981025
AGGTAAG12250.029.1177162
AACGCAG139200.029.0017326
GTGGTAT25750.028.0736241
TAAGGTG14300.027.2659825
TATAACG700.00834237827.1765352
GTGTAAG7550.026.4566251
TAAGGTA6900.026.187864
TGGTATC26350.025.809972
CGCAGAG157350.025.4753388
ACGCAGA157850.025.454827
GTACATG91250.024.9651811
TACATGG90400.024.8842662
AAGGTAA13600.024.828561
GTAAGGT14900.024.5736684
Produced by FastQC (version 0.11.5)