##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938027_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1959101 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49656449565387 33.0 31.0 34.0 31.0 34.0 2 32.720439630218145 34.0 31.0 34.0 31.0 34.0 3 32.77418315849974 34.0 31.0 34.0 31.0 34.0 4 36.241319360257584 37.0 35.0 37.0 35.0 37.0 5 36.16786934415326 37.0 37.0 37.0 35.0 37.0 6 36.227855531695404 37.0 37.0 37.0 35.0 37.0 7 36.19689541274288 37.0 37.0 37.0 35.0 37.0 8 36.19201051911055 37.0 37.0 37.0 35.0 37.0 9 37.88518815517934 39.0 38.0 39.0 35.0 39.0 10-11 37.86275694821247 39.0 38.0 39.0 35.0 39.0 12-13 37.77970482379418 39.0 38.0 39.0 35.0 39.0 14-15 39.01391658725099 40.0 38.0 41.0 36.0 41.0 16-17 38.93066615758963 40.0 38.0 41.0 35.5 41.0 18-19 39.18981742135806 40.0 39.0 41.0 36.0 41.0 20-21 39.16095060948874 40.0 39.0 41.0 36.0 41.0 22-23 39.055746743021416 40.0 39.0 41.0 35.0 41.0 24-25 38.935738126824496 40.0 38.0 41.0 35.0 41.0 26-27 38.76337105641822 40.0 38.0 41.0 35.0 41.0 28-29 38.63184338122434 40.0 38.0 41.0 34.5 41.0 30-31 38.36046278369517 40.0 38.0 41.0 34.0 41.0 32-33 38.093819052718565 40.0 37.0 41.0 34.0 41.0 34-35 37.972374063409696 40.0 37.0 41.0 33.0 41.0 36-37 37.90122382664293 40.0 37.0 41.0 33.0 41.0 38-39 37.698419836445396 40.0 37.0 41.0 33.0 41.0 40-41 37.46156859702486 40.0 36.5 41.0 33.0 41.0 42-43 37.16891421116114 40.0 36.0 41.0 32.0 41.0 44-45 36.85521777590844 39.5 36.0 41.0 31.0 41.0 46-47 36.41458173927735 39.0 35.0 41.0 30.5 41.0 48-49 35.9262924678207 39.0 35.0 40.5 29.5 41.0 50-51 35.501378948813766 38.5 35.0 40.0 28.0 41.0 52-53 34.94737075832231 38.0 34.0 40.0 26.5 41.0 54-55 34.53778671952084 38.0 34.0 40.0 25.5 41.0 56-57 34.12788595381249 37.5 33.5 40.0 24.0 41.0 58-59 33.60061426133721 37.0 33.0 39.5 22.5 41.0 60-61 33.09175688236594 36.0 32.5 39.0 21.5 40.5 62-63 32.51915546977925 35.5 31.5 39.0 19.5 40.0 64-65 31.932871250639963 35.0 31.0 38.0 17.5 40.0 66-67 31.36464505913682 35.0 31.0 38.0 11.5 40.0 68-69 30.731997992956977 35.0 30.0 37.0 7.5 39.5 70-71 30.109948644812086 34.0 29.0 36.5 4.5 39.0 72-73 29.46386225110395 34.0 28.5 36.0 2.0 39.0 74-75 28.8334590712781 34.0 27.5 35.0 2.0 38.0 76-77 27.55794520037507 31.5 26.0 34.5 2.0 36.0 78-79 28.221629972114762 33.0 27.0 35.0 2.0 37.0 80-81 27.978934725672644 33.0 27.0 35.0 2.0 36.0 82-83 27.452648178935135 33.0 26.0 35.0 2.0 36.0 84-85 26.834511594859073 33.0 25.0 35.0 2.0 35.5 86-87 26.157009260880372 32.0 23.5 35.0 2.0 35.0 88-89 25.36267808550963 32.0 20.5 34.0 2.0 35.0 90-91 24.72756151928869 31.0 18.5 34.0 2.0 35.0 92-93 23.81103271347419 31.0 8.5 34.0 2.0 35.0 94-95 22.924398997295185 30.5 2.0 34.0 2.0 35.0 96-97 21.886438984003377 30.0 2.0 34.0 2.0 35.0 98-99 20.469057746384692 29.0 2.0 34.0 2.0 35.0 100-101 16.617359697126385 20.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1242287267720954 1101 2 0.09647259899242755 1101 3 0.1569541913039103 1101 4 0.007360192075253735 1101 5 -0.09718859764947041 1101 6 0.020794610358343846 1101 7 0.02989275777565581 1101 8 0.06782577699432579 1101 9 -0.011750638735428254 1101 10-11 0.147450323861257 1101 12-13 0.12322704811690954 1101 14-15 0.21959522713247281 1101 16-17 0.30027722638335064 1101 18-19 0.11172645604870723 1101 20-21 0.10036979948409197 1101 22-23 0.2136238067424614 1101 24-25 0.2630526956928918 1101 26-27 0.2535100349582322 1101 28-29 0.35134501890011194 1101 30-31 0.3971605105564109 1101 32-33 0.47770616347142436 1101 34-35 0.3881059332458605 1101 36-37 0.3625440261066615 1101 38-39 0.4878846689395431 1101 40-41 0.6366641550412027 1101 42-43 0.6318326242714463 1101 44-45 0.6568067158580035 1101 46-47 0.7696350244941854 1101 48-49 0.8646212747705917 1101 50-51 1.0352837548892282 1101 52-53 0.9668162236929874 1101 54-55 1.0870671976578663 1101 56-57 1.1355627150225374 1101 58-59 1.3188081830023677 1101 60-61 1.2461039008897856 1101 62-63 1.195354157459711 1101 64-65 1.4829829767138243 1101 66-67 1.624261556673531 1101 68-69 1.8134327403985928 1101 70-71 1.6510289733224397 1101 72-73 2.039852978183365 1101 74-75 1.922585377973629 1101 76-77 1.906523759000219 1101 78-79 2.0888927468279768 1101 80-81 2.1147542200694147 1101 82-83 2.195890160793791 1101 84-85 2.457960799475078 1101 86-87 2.574012584644887 1101 88-89 2.7241116194064077 1101 90-91 2.9515855897223737 1101 92-93 3.053891580737808 1101 94-95 3.4279327438236926 1101 96-97 3.80591334545025 1101 98-99 4.284844675449637 1101 100-101 3.9754170210142803 1102 1 0.22622275228806643 1102 2 0.16577859159642117 1102 3 0.2727560762906265 1102 4 0.1126646202372541 1102 5 0.00693739480139044 1102 6 0.09283167716996843 1102 7 0.04614649342324384 1102 8 0.15449375799644116 1102 9 0.21023296142075765 1102 10-11 0.2077521616339837 1102 12-13 0.34908777832595916 1102 14-15 0.4380207106930669 1102 16-17 0.4086972994757119 1102 18-19 0.4514915792006633 1102 20-21 0.3144893989052733 1102 22-23 0.3662553168500864 1102 24-25 0.40065277021443535 1102 26-27 0.33296448366469633 1102 28-29 0.20076476532269538 1102 30-31 0.40455715657653 1102 32-33 0.3325220225236194 1102 34-35 0.35938770379606666 1102 36-37 0.3388855095957979 1102 38-39 0.43764196350126383 1102 40-41 0.43638479495667326 1102 42-43 0.5861017261592423 1102 44-45 0.5614066985036743 1102 46-47 0.6015106258345568 1102 48-49 0.8411618226570639 1102 50-51 0.776469572571763 1102 52-53 0.6496985038990957 1102 54-55 0.8844264999626787 1102 56-57 0.8817426883785373 1102 58-59 1.084012045362094 1102 60-61 1.0324259756001837 1102 62-63 1.1692700221265397 1102 64-65 1.2738846491099771 1102 66-67 1.4620591643278118 1102 68-69 1.3767026793777042 1102 70-71 1.4922424175703668 1102 72-73 1.736857665129083 1102 74-75 1.8095431188989757 1102 76-77 1.8577746745688408 1102 78-79 1.8648411419350772 1102 80-81 2.0709820645848076 1102 82-83 2.195108099303308 1102 84-85 2.4600938829570715 1102 86-87 2.359731883427788 1102 88-89 2.637799338409149 1102 90-91 2.7950625939563203 1102 92-93 2.885839917146434 1102 94-95 3.0620343572661533 1102 96-97 3.0416896519379613 1102 98-99 3.550014881889627 1102 100-101 3.2661508923319076 1103 1 0.21799130271853784 1103 2 0.19537106877580612 1103 3 0.1644457746217043 1103 4 0.10731258751699357 1103 5 0.008643206121590197 1103 6 0.03303438028600425 1103 7 0.10908563966727769 1103 8 0.1815282828590128 1103 9 0.23866651461013788 1103 10-11 0.164646857071574 1103 12-13 0.22998211039545424 1103 14-15 0.30787039620226153 1103 16-17 0.30932147899178375 1103 18-19 0.24797057909422193 1103 20-21 0.27511901670811056 1103 22-23 0.38876379849754983 1103 24-25 0.4009415071493976 1103 26-27 0.3904762013680596 1103 28-29 0.3691445840791019 1103 30-31 0.29761422616579836 1103 32-33 0.4274054449861353 1103 34-35 0.47587990484017695 1103 36-37 0.4292535941321063 1103 38-39 0.49985819687900346 1103 40-41 0.6691757371811491 1103 42-43 0.6120227480432945 1103 44-45 0.8036655511658637 1103 46-47 0.6544043877709456 1103 48-49 0.8140665835131458 1103 50-51 0.9323885879788136 1103 52-53 1.0212111795728873 1103 54-55 1.1405456430991165 1103 56-57 0.9020224818092828 1103 58-59 1.1494144785825071 1103 60-61 1.1576361051280628 1103 62-63 1.4212451479289783 1103 64-65 1.343657440058319 1103 66-67 1.3879331441137879 1103 68-69 1.5515561392659016 1103 70-71 1.5831145682812604 1103 72-73 1.7330728867040932 1103 74-75 1.9252189651979421 1103 76-77 1.852039851303985 1103 78-79 1.903425755926829 1103 80-81 2.128180423188738 1103 82-83 2.2005125879391834 1103 84-85 2.2943955861379486 1103 86-87 2.552819967768336 1103 88-89 2.678514304892417 1103 90-91 2.811438015591772 1103 92-93 3.015537397916674 1103 94-95 3.2327509876571803 1103 96-97 3.3095885891168813 1103 98-99 3.6869222218027353 1103 100-101 3.436892909550906 1104 1 0.20785390497825063 1104 2 0.14065997889697002 1104 3 0.15180945690857328 1104 4 0.14188259956301152 1104 5 -0.052404614509327985 1104 6 0.024885603973913817 1104 7 0.10379407805087482 1104 8 0.1548756127347346 1104 9 0.17634209045318983 1104 10-11 0.17157788848036404 1104 12-13 0.1731363954174796 1104 14-15 0.3004582098387232 1104 16-17 0.2870783483073538 1104 18-19 0.2165697895886538 1104 20-21 0.24762232536272677 1104 22-23 0.2181060791034639 1104 24-25 0.244658720610758 1104 26-27 0.3189426885254534 1104 28-29 0.31159342279276103 1104 30-31 0.28307757495856833 1104 32-33 0.31295456736406635 1104 34-35 0.31758278194108414 1104 36-37 0.24604755923751043 1104 38-39 0.3869425007129337 1104 40-41 0.39932066653937426 1104 42-43 0.5772473022606661 1104 44-45 0.6400785809791856 1104 46-47 0.5627337971960955 1104 48-49 0.6402093551028258 1104 50-51 0.6599854532714247 1104 52-53 0.7357874317757975 1104 54-55 0.908547238567806 1104 56-57 0.9895692544138512 1104 58-59 1.0250082077962688 1104 60-61 0.983129717538894 1104 62-63 1.1121473763278615 1104 64-65 1.0272323413701265 1104 66-67 1.1992321139164517 1104 68-69 1.4404704891124105 1104 70-71 1.6200792119637306 1104 72-73 1.716102745740546 1104 74-75 1.6219941984038044 1104 76-77 1.679845521846559 1104 78-79 1.6624802692974505 1104 80-81 1.8530003615186175 1104 82-83 2.009614359640871 1104 84-85 2.130158313886529 1104 86-87 2.3103604430717155 1104 88-89 2.6578189274086412 1104 90-91 2.8923669075181984 1104 92-93 3.146702124344081 1104 94-95 3.338531361563728 1104 96-97 3.566617337268262 1104 98-99 4.090596085602741 1104 100-101 3.8930935225948886 1105 1 0.25321493664065287 1105 2 0.21605397165729556 1105 3 0.20135739509990458 1105 4 0.14411174960479656 1105 5 0.036845826707534 1105 6 0.015010493568532013 1105 7 0.12168042404150725 1105 8 0.10187914193004843 1105 9 0.23932266072496589 1105 10-11 0.19064584855583888 1105 12-13 0.28666266636751914 1105 14-15 0.39032238896494675 1105 16-17 0.3632394460106454 1105 18-19 0.33416979649489775 1105 20-21 0.3220594265342456 1105 22-23 0.469271371806542 1105 24-25 0.43238320705376054 1105 26-27 0.3660503778116677 1105 28-29 0.45824967075258627 1105 30-31 0.3947167704466352 1105 32-33 0.39489775061082355 1105 34-35 0.44240978878311665 1105 36-37 0.40605537191876806 1105 38-39 0.3662913666062053 1105 40-41 0.3532405498671167 1105 42-43 0.4295834450298557 1105 44-45 0.5224818113718044 1105 46-47 0.33357323418666596 1105 48-49 0.3065242352930895 1105 50-51 0.3366308239070719 1105 52-53 0.53428189896227 1105 54-55 0.617311739643398 1105 56-57 0.6369208471918668 1105 58-59 0.7576912217430873 1105 60-61 0.7386868108114086 1105 62-63 0.9653770135831294 1105 64-65 1.0254512874459003 1105 66-67 1.0627463798832153 1105 68-69 1.3810575570746515 1105 70-71 1.4902215775767118 1105 72-73 1.4419033971929878 1105 74-75 1.7540317489124106 1105 76-77 1.6796990340730282 1105 78-79 1.6393231960961892 1105 80-81 1.79063892465431 1105 82-83 2.031115959136283 1105 84-85 2.0477879420773597 1105 86-87 2.189205477352015 1105 88-89 2.5020573717354644 1105 90-91 2.582632673568316 1105 92-93 2.7935960439954 1105 94-95 3.047594773234298 1105 96-97 3.384337043299947 1105 98-99 3.6460483173695977 1105 100-101 3.3409739551195976 1106 1 0.1083423012019793 1106 2 0.10307619951944957 1106 3 0.07751831386581642 1106 4 0.09150266717222166 1106 5 -0.010929624598048804 1106 6 0.05427669655963285 1106 7 0.07342662289664759 1106 8 0.1409343696645209 1106 9 0.19083438460430813 1106 10-11 0.1697476788445229 1106 12-13 0.10839353557252451 1106 14-15 0.07016103989306544 1106 16-17 0.09345992820922078 1106 18-19 -0.017606769219590035 1106 20-21 -0.0027539530569740123 1106 22-23 -0.002559384260365505 1106 24-25 -0.006174035520523091 1106 26-27 -0.09881649758472832 1106 28-29 0.05064064445823391 1106 30-31 0.1791646824052009 1106 32-33 0.2926560862876073 1106 34-35 0.23154680859573062 1106 36-37 0.2777215777298281 1106 38-39 0.15300439038557556 1106 40-41 0.14151943826774271 1106 42-43 0.26619159478469356 1106 44-45 0.4548346058670276 1106 46-47 0.47929133729977735 1106 48-49 0.5211672758643147 1106 50-51 0.6053636903271027 1106 52-53 0.6313551917141993 1106 54-55 0.8113489208990856 1106 56-57 0.8666773404906607 1106 58-59 1.1084404580674914 1106 60-61 1.008242036848678 1106 62-63 0.7860523537350375 1106 64-65 1.0390107559658013 1106 66-67 1.217186142661383 1106 68-69 1.1408449942814372 1106 70-71 0.954699271512716 1106 72-73 1.4451907467980512 1106 74-75 1.6936391678259781 1106 76-77 1.7882779198886745 1106 78-79 1.9367770276340117 1106 80-81 1.604825600679085 1106 82-83 2.0698205188390197 1106 84-85 2.3206245122359057 1106 86-87 2.352799033520128 1106 88-89 2.4178787665586867 1106 90-91 2.3981998275408287 1106 92-93 2.5332459190682854 1106 94-95 2.8233531418106814 1106 96-97 3.1392186530202615 1106 98-99 3.307417625117484 1106 100-101 3.00427298147401 1107 1 0.14839217686581918 1107 2 0.1227503133222001 1107 3 0.13670303357859126 1107 4 0.05501968349531694 1107 5 -0.0026526756375133687 1107 6 0.0771725805683019 1107 7 -0.011111921313968764 1107 8 0.10966351233422245 1107 9 0.14623248570136838 1107 10-11 0.13420936761146862 1107 12-13 0.1500766980284851 1107 14-15 0.29994901401463636 1107 16-17 0.21270181160205226 1107 18-19 0.13242847610469966 1107 20-21 0.15731819938964264 1107 22-23 0.23441437290749434 1107 24-25 0.12552793892362502 1107 26-27 0.2890266623513895 1107 28-29 0.2645327872490455 1107 30-31 0.30291853753225695 1107 32-33 0.2564321475981757 1107 34-35 0.3305003645991391 1107 36-37 0.36104024813934643 1107 38-39 0.2127158892133565 1107 40-41 0.3392218124123332 1107 42-43 0.4272109992636146 1107 44-45 0.45570284103208536 1107 46-47 0.4707211298628451 1107 48-49 0.5703936980185844 1107 50-51 0.52021133983326 1107 52-53 0.5935501010728927 1107 54-55 0.6484606724722184 1107 56-57 0.5666042702536274 1107 58-59 0.6849114072854476 1107 60-61 0.6168594100238849 1107 62-63 0.8197216852523397 1107 64-65 1.0407096186116824 1107 66-67 1.2233741680488777 1107 68-69 1.29474649962064 1107 70-71 1.2851459096029743 1107 72-73 1.5296638509227307 1107 74-75 1.448769426181265 1107 76-77 1.3490121576528722 1107 78-79 1.252973204521009 1107 80-81 1.5463960215430141 1107 82-83 1.7826819123924373 1107 84-85 1.8041818081537997 1107 86-87 2.0174454751272073 1107 88-89 2.2184157554132327 1107 90-91 2.1974130802172205 1107 92-93 2.4905139502557816 1107 94-95 2.545221100552471 1107 96-97 2.6752906675267845 1107 98-99 3.0397589845008604 1107 100-101 3.04137302986166 1108 1 0.21502382126833197 1108 2 0.22092343766553313 1108 3 0.17555987828701802 1108 4 0.1638585167535993 1108 5 0.05295767665787565 1108 6 0.11916534739864204 1108 7 0.19615198216753527 1108 8 0.14201443542104641 1108 9 0.2641485180362082 1108 10-11 0.25156700925509057 1108 12-13 0.2763614433308561 1108 14-15 0.3812694605842353 1108 16-17 0.3337868227877294 1108 18-19 0.29523554631362714 1108 20-21 0.2824370249287327 1108 22-23 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2316 52-53 0.0997985295544126 2316 54-55 0.5067095877397279 2316 56-57 0.3825597463828885 2316 58-59 0.458363671716306 2316 60-61 0.6386950525127304 2316 62-63 0.5944302174079183 2316 64-65 0.7441415308424659 2316 66-67 0.8545142179870595 2316 68-69 1.1482416750885243 2316 70-71 1.2715057211580785 2316 72-73 1.3100760184888607 2316 74-75 1.7067198298869002 2316 76-77 1.7669192288822586 2316 78-79 1.7582016553624875 2316 80-81 1.7783857687315638 2316 82-83 2.0263523923738376 2316 84-85 2.1581494454092187 2316 86-87 2.295411637818848 2316 88-89 2.800746986759222 2316 90-91 3.1075797570647232 2316 92-93 3.1123047970692497 2316 94-95 3.600373763361784 2316 96-97 3.982578464588169 2316 98-99 4.2663119288694595 2316 100-101 4.378095989444692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 39.0 6 71.0 7 204.0 8 521.0 9 988.0 10 1761.0 11 2965.0 12 4589.0 13 6841.0 14 8907.0 15 12682.0 16 17034.0 17 24596.0 18 25242.0 19 19588.0 20 18183.0 21 19122.0 22 20456.0 23 22572.0 24 25158.0 25 28361.0 26 32016.0 27 37638.0 28 45266.0 29 54288.0 30 65675.0 31 78391.0 32 95774.0 33 120078.0 34 159391.0 35 220547.0 36 297618.0 37 336730.0 38 150498.0 39 5295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41307518091206 18.05021793159209 14.700671379372476 22.83603550812337 2 16.36439099697367 19.695396383923285 38.83320876778122 25.107003851321824 3 19.616996275218334 23.46260236513682 29.476973682531405 27.443427677113448 4 13.417523155178618 16.236214724881513 34.96525128058538 35.38101083935449 5 13.984145941965215 37.0223583000762 33.180066877120574 15.813428880838012 6 33.46973943660893 35.10079368036666 16.029648292762854 15.399818590261555 7 29.773043860423737 32.94899037875025 18.857322823070376 18.420642937755634 8 28.027402420605004 33.41325110497336 18.581724131066508 19.97762234335513 9 27.26107536058631 16.114227903512887 19.11534933625168 37.50934739964912 10-11 25.931588008989838 25.995724569585743 25.52951583404837 22.543171587376047 12-13 27.056057855108033 23.308522633595715 26.52706522022091 23.108354291075344 14-15 23.466248039279243 24.42898553979606 25.270238747262137 26.83452767366256 16-17 22.652558494942323 27.798847532618275 26.253266166471256 23.295327805968146 18-19 22.754110176045035 25.619123261128447 29.24264752046985 22.384119042356673 20-21 23.060092358689012 24.49804782908079 30.040998396713597 22.40086141551661 22-23 23.174880270124383 24.481910675706544 29.90420333852705 22.439005715642026 24-25 22.8950723232117 24.805068686314417 29.818346551647128 22.48151243882675 26-27 21.98922799068488 25.35485557176592 29.729408269464606 22.92650816808459 28-29 22.472577983473034 25.72432457540474 29.4924049347124 22.310692506409826 30-31 22.35770386519123 25.20283538214722 29.32227588062075 23.1171848720408 32-33 22.091433774981482 25.148141928364083 29.423419211158585 23.337005085495846 34-35 23.029395894284434 25.35414387514492 29.231565604613657 22.38489462595699 36-37 23.16843160535066 24.783547021417128 29.10033046022482 22.947690913007392 38-39 22.5107046158998 25.22963621662018 29.02329185571178 23.236367311768237 40-41 22.964886187488244 25.037680557189834 29.1892165073129 22.808216748009023 42-43 22.612701714533657 25.09533623874578 28.917310600501533 23.37465144621903 44-45 22.5879974201393 25.397054293204178 29.183768112686927 22.831180173969592 46-47 23.251455642154234 25.748493824463363 28.229172462267133 22.770878071115273 48-49 22.81691959730509 25.876179941718163 28.01152161118799 23.295378849788754 50-51 23.14464644752874 26.14196511563212 27.946721480924158 22.76666695591498 52-53 23.561393720895452 25.72789764284741 27.868573391570926 22.842135244686208 54-55 22.87120470052335 26.174428985539794 27.91948960262896 23.03487671130789 56-57 22.85706556221451 26.345349218850888 27.72669709218667 23.07088812674793 58-59 22.931793715586892 25.734073945141162 27.632699896534174 23.701432442737765 60-61 22.403643303739827 26.2524494653415 28.048298683937173 23.295608546981498 62-63 22.599498443418693 26.269472579514787 28.215288543061334 22.915740434005187 64-65 22.25952107624875 26.411731707553614 27.931255203279466 23.39749201291817 66-67 22.21488892478972 26.32236069563557 27.924090673245196 23.53865970632951 68-69 22.893099128879488 25.838018341206055 28.081870831602085 23.187011698312375 70-71 22.668484166972505 26.216897444286946 27.474030180169372 23.640588208571174 72-73 22.6091712474242 26.42839751498264 27.26385724880953 23.69857398878363 74-75 22.50108595728347 26.93911646209154 27.233001259251054 23.326796321373937 76-77 22.699799550916467 26.315565149525217 27.600006329433757 23.384628970124563 78-79 22.72710799494258 26.5820649369277 27.631347235288022 23.059479832841696 80-81 22.943358896962046 26.599504823264375 27.26578066391251 23.19135561586107 82-83 22.79460914240649 26.676909687438986 27.329800171766273 23.198680998388255 84-85 22.590274729201333 26.85495809129054 27.260211738522322 23.294555440985796 86-87 23.045551914052243 26.82159035716537 26.856760456149548 23.27609727263284 88-89 22.727414257866236 27.102354600400897 26.541970015838896 23.628261125893967 90-91 22.573392640930408 26.858909309196488 26.86485606303457 23.702841986838532 92-93 22.741757571457523 26.461397345006716 26.983652195573377 23.813192887962387 94-95 22.550655262453358 26.168642190637996 27.264042860486228 24.016659686422418 96-97 22.890610883171743 25.806850907917827 27.166634849303094 24.13590335960733 98-99 22.421049246567687 26.28973697629678 26.76564403774997 24.523569739385565 100-101 22.778917472861277 25.494167988276256 27.10171655264328 24.625197986219188 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1908.0 1 1251.0 2 936.0 3 1264.5 4 1003.5 5 922.5 6 1791.0 7 3860.5 8 6115.5 9 6773.5 10 5220.0 11 3510.0 12 3167.5 13 3206.0 14 3293.5 15 3437.5 16 3551.5 17 3684.5 18 3857.5 19 4068.0 20 4351.0 21 4923.5 22 5723.0 23 6575.0 24 7633.0 25 9077.0 26 11015.0 27 13183.5 28 14906.5 29 16604.5 30 18676.0 31 22268.5 32 28403.0 33 29655.0 34 27090.0 35 27781.5 36 30169.5 37 33111.5 38 36444.5 39 39076.0 40 41654.5 41 45737.0 42 48564.0 43 51595.0 44 55520.0 45 60267.0 46 64023.0 47 68099.0 48 70538.5 49 70069.5 50 71027.5 51 71653.0 52 72907.5 53 76686.0 54 80958.0 55 78030.0 56 72579.5 57 68458.0 58 64881.5 59 58781.5 60 47509.5 61 39471.5 62 33679.5 63 25116.0 64 17974.5 65 13807.0 66 10329.5 67 8033.0 68 6313.0 69 4857.5 70 3600.5 71 2408.5 72 1644.5 73 1225.0 74 868.0 75 610.0 76 428.0 77 264.5 78 169.0 79 104.5 80 51.0 81 24.5 82 20.0 83 11.5 84 4.5 85 4.5 86 2.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014955839438599643 3 0.003062629236573306 4 0.0018375775419439836 5 0.12138220540952203 6 0.0 7 0.0 8 2.0417528243822037E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.7865337213344282E-4 24-25 6.890915782289938E-4 26-27 0.006890915782289938 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01133172817532123 36-37 7.656573091433265E-4 38-39 0.006788828141070828 40-41 0.010183242211606242 42-43 0.0057934736391845034 44-45 0.004109027559069185 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.5521910304777546E-5 68-69 7.656573091433265E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2760955152388775E-4 82-83 0.002220406196515647 84-85 0.004134549469373963 86-87 0.0025777129407825324 88-89 0.0 90-91 0.0025011472098681996 92-93 0.0 94-95 2.5521910304777546E-5 96-97 1.5313146182866527E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1959101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.5020810045282 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02435319594855 32.05233600224852 2 14.355021097476023 12.776566234031787 3 5.336201600197989 7.124162276055117 4 2.516429465028324 4.479453915794881 5 1.4519645303946054 3.230772157366123 6 0.8969507900101515 2.39497060284644 7 0.6055933039580267 1.8865113587977977 8 0.42681707090862403 1.5195398290952833 9 0.3363326223079844 1.347075144217378 >10 1.8200114187664047 14.996198504985811 >50 0.14085545608879957 4.305040470789025 >100 0.07835852695989273 6.539914452354678 >500 0.005612733770924984 1.7183729802222447 >1k 0.005154551422278047 3.9735145003952526 >5k 1.1454558716173439E-4 0.38348620380338017 >10k+ 2.2909117432346878E-4 1.272085366996325 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13663 0.6974117209883512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11292 0.5763868223230961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7523 0.384002662445683 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3442 0.17569283053808865 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3414 0.1742636035610211 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3240 0.16538197877495853 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3153 0.1609411663819272 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2895 0.14777186066466202 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2665 0.13603178192446433 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2491 0.12715015713840175 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2424 0.12373022115756155 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2393 0.12214786271866536 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2197 0.11214327387919255 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2155 0.10999943341359122 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.10438206095551E-5 2 0.0 0.0 0.0 0.0 5.10438206095551E-5 3 0.0 0.0 0.0 0.0 5.10438206095551E-5 4 0.0 0.0 0.0 0.0 5.10438206095551E-5 5 0.0 0.0 0.0 0.0 5.10438206095551E-5 6 0.0 0.0 0.0 0.0 5.10438206095551E-5 7 5.10438206095551E-5 0.0 0.0 0.0 5.10438206095551E-5 8 5.10438206095551E-5 0.0 0.0 0.0 5.10438206095551E-5 9 5.10438206095551E-5 0.0 0.0 0.0 1.020876412191102E-4 10-11 5.10438206095551E-5 0.0 0.0 2.552191030477755E-5 1.020876412191102E-4 12-13 5.10438206095551E-5 0.0 0.0 1.020876412191102E-4 1.020876412191102E-4 14-15 2.041752824382204E-4 0.0 0.0 1.020876412191102E-4 1.2760955152388775E-4 16-17 2.041752824382204E-4 0.0 0.0 1.531314618286653E-4 1.531314618286653E-4 18-19 2.552191030477755E-4 0.0 0.0 1.531314618286653E-4 1.531314618286653E-4 20-21 3.062629236573306E-4 0.0 0.0 2.2969719274299795E-4 1.531314618286653E-4 22-23 3.062629236573306E-4 0.0 0.0 4.083505648764408E-4 2.8074101335255305E-4 24-25 3.573067442668857E-4 0.0 0.0 9.443106812767693E-4 4.083505648764408E-4 26-27 3.573067442668857E-4 0.0 0.0 0.0019141432728583163 4.3387247518121834E-4 28-29 6.380477576194387E-4 0.0 0.0 0.006074214652537057 4.593943854859959E-4 30-31 6.635696679242163E-4 0.0 0.0 0.019090388907973606 4.593943854859959E-4 32-33 6.635696679242163E-4 0.0 0.0 0.04246845874714984 4.593943854859959E-4 34-35 7.146134885337714E-4 0.0 0.0 0.07500889438574121 5.10438206095551E-4 36-37 8.677449503624367E-4 0.0 0.0 0.12794133635784985 5.10438206095551E-4 38-39 0.001020876412191102 0.0 0.0 0.21022397518045266 5.10438206095551E-4 40-41 0.001020876412191102 0.0 0.0 0.2962583348178578 5.359601164003285E-4 42-43 0.0010463983224958794 0.0 0.0 0.3837474433426352 5.870039370098836E-4 44-45 0.0011229640534102122 0.0 0.0 0.4871367019872891 6.380477576194387E-4 46-47 0.0012505736049340998 0.0 0.0 0.607727728177363 6.635696679242163E-4 48-49 0.0013271393358484326 0.0 0.0 0.7326064352986396 7.656573091433265E-4 50-51 0.0013271393358484326 0.0 0.0 0.8691231335188947 7.656573091433265E-4 52-53 0.0013271393358484326 0.0 0.0 0.9944867569359619 8.677449503624367E-4 54-55 0.0013271393358484326 0.0 0.0 1.127328300072329 9.187887709719918E-4 56-57 0.0014292269770675428 0.0 0.0 1.2841093950745777 9.187887709719918E-4 58-59 0.0014292269770675428 0.0 0.0 1.4437999878515706 9.187887709719918E-4 60-61 0.0014292269770675428 0.0 0.0 1.614924396445104 9.187887709719918E-4 62-63 0.0014292269770675428 0.0 0.0 1.7758655628270312 9.187887709719918E-4 64-65 0.0014292269770675428 0.0 0.0 1.9470410152411743 9.443106812767693E-4 66-67 0.0014802707976770979 0.0 0.0 2.1405481391719974 9.953545018863245E-4 68-69 0.0014802707976770979 0.0 0.0 2.3590156913808933 0.001020876412191102 70-71 0.001582358438896208 0.0 0.0 2.5821027093549542 0.0011229640534102122 72-73 0.0016334022595057632 0.0 0.0 2.8128973442410574 0.0012250516946293224 74-75 0.0016334022595057632 0.0 0.0 3.0449170308217903 0.0012250516946293224 76-77 0.0016334022595057632 0.0 0.0 3.2855886449958422 0.0012250516946293224 78-79 0.0016334022595057632 0.0 0.0 3.558494431884829 0.0012250516946293224 80-81 0.0017354899007248734 0.0 0.0 3.843012687962489 0.0013271393358484326 82-83 0.0018886213625535387 0.0 0.0 4.135008863759449 0.0014802707976770979 84-85 0.0019141432728583163 0.0 0.0 4.433844911518089 0.0014802707976770979 86-87 0.0019396651831630938 0.0 0.0 4.737019684028542 0.0014802707976770979 88-89 0.0019396651831630938 0.0 0.0 5.053593459449003 0.0015057927079818755 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5760 0.0 53.503796 1 GTATCAA 9915 0.0 40.96352 1 TATCAAC 12910 0.0 31.386686 2 ATCAACG 13145 0.0 30.786242 3 TCAACGC 13310 0.0 30.40304 4 CAACGCA 13550 0.0 29.898102 5 AGGTAAG 1225 0.0 29.117716 2 AACGCAG 13920 0.0 29.001732 6 GTGGTAT 2575 0.0 28.073624 1 TAAGGTG 1430 0.0 27.265982 5 TATAACG 70 0.008342378 27.176535 2 GTGTAAG 755 0.0 26.456625 1 TAAGGTA 690 0.0 26.18786 4 TGGTATC 2635 0.0 25.80997 2 CGCAGAG 15735 0.0 25.475338 8 ACGCAGA 15785 0.0 25.45482 7 GTACATG 9125 0.0 24.965181 1 TACATGG 9040 0.0 24.884266 2 AAGGTAA 1360 0.0 24.82856 1 GTAAGGT 1490 0.0 24.573668 4 >>END_MODULE