FastQCFastQC Report
Thu 26 May 2016
SRR938026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920785
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140990.7340228083830309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109910.5722139646030139No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72870.3793761404842291No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33270.17321043219308774No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33110.17237743943231543No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30280.1576438799761556No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29500.15358304026739067No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26390.1373917434798793No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.13385152424659708No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24900.12963449839518737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.12380354906978137No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22840.11890971660024417No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21220.11047566489742475No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA21210.11042360284987647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53700.054.137771
GTATCAA97000.043.2588041
TATCAAC123300.033.9203222
ATCAACG125450.033.301973
TCAACGC126600.032.9243774
CAACGCA129000.032.274995
AACGCAG131550.031.7913956
GTGTAAG6300.031.0670971
GTGGTAT25150.029.040951
TAAGGTG12100.027.888475
ACGCAGA148850.027.8613477
CGCAGAG150150.027.5568478
TGGTATC25350.027.1850662
TAAGGTA7550.027.0690634
AGGTAAG11350.026.3806252
GTACATG89750.025.4244061
TACATGG87800.025.2791652
GCAGAGT163600.024.7660759
TGTAAGG8200.024.3430982
TATACCG1000.001648336323.7641185
Produced by FastQC (version 0.11.5)