##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938026_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1920785 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.923516166567314 33.0 31.0 34.0 30.0 34.0 2 32.096478262793596 34.0 31.0 34.0 30.0 34.0 3 32.168459249733836 34.0 31.0 34.0 30.0 34.0 4 35.6985039970637 37.0 35.0 37.0 35.0 37.0 5 35.70897367482566 37.0 35.0 37.0 35.0 37.0 6 35.74553112399357 37.0 36.0 37.0 35.0 37.0 7 35.68695507305607 37.0 35.0 37.0 33.0 37.0 8 35.659103439479175 37.0 35.0 37.0 33.0 37.0 9 37.30260128020575 39.0 37.0 39.0 34.0 39.0 10-11 37.22568194774533 39.0 37.0 39.0 34.0 39.0 12-13 37.11959277066408 39.0 37.0 39.0 33.5 39.0 14-15 38.30300658324591 40.0 38.0 41.0 33.5 41.0 16-17 38.16032273263275 40.0 38.0 41.0 33.0 41.0 18-19 38.317721140054715 40.0 38.0 41.0 34.0 41.0 20-21 38.302635641157124 40.0 38.0 41.0 34.0 41.0 22-23 38.218242020840435 40.0 38.0 41.0 34.0 41.0 24-25 38.10296128926454 40.0 38.0 41.0 33.0 41.0 26-27 37.9173202622886 40.0 38.0 41.0 33.0 41.0 28-29 37.703228888188946 40.0 38.0 41.0 32.5 41.0 30-31 37.458853801961176 40.0 37.0 41.0 32.0 41.0 32-33 37.16559713866987 40.0 36.5 41.0 31.5 41.0 34-35 36.915078470521166 39.5 36.0 41.0 31.0 41.0 36-37 36.63994122194832 39.0 35.5 41.0 30.0 41.0 38-39 36.334633496200766 39.0 35.0 40.5 30.0 41.0 40-41 36.09185020707679 39.0 35.0 40.0 30.0 41.0 42-43 35.87941570764036 39.0 35.0 40.5 28.5 41.0 44-45 35.57421653126195 39.0 35.0 40.0 27.5 41.0 46-47 35.13543160738969 38.0 35.0 40.0 25.5 41.0 48-49 34.68080498337919 38.0 34.0 40.0 24.0 41.0 50-51 33.92160991469633 37.5 33.5 39.5 23.0 40.5 52-53 34.00507422746429 38.0 33.5 39.5 23.0 40.5 54-55 34.081300093451375 38.0 34.0 40.0 22.5 41.0 56-57 33.75167574715546 37.5 33.5 40.0 21.5 41.0 58-59 33.404407052324956 37.0 33.0 40.0 19.5 41.0 60-61 32.95637981346168 36.5 33.0 39.5 18.0 41.0 62-63 32.39927503598789 36.0 32.0 39.0 13.0 41.0 64-65 31.861096634969556 35.0 31.0 39.0 8.0 40.0 66-67 31.329068583938337 35.0 31.0 38.0 6.5 40.0 68-69 30.715193267336012 35.0 30.0 37.5 2.0 40.0 70-71 30.161678168040673 34.5 29.5 37.0 2.0 39.0 72-73 29.54263230918609 34.0 29.0 36.0 2.0 39.0 74-75 28.881486735891833 34.0 28.0 35.5 2.0 38.5 76-77 28.243606910716192 34.0 26.5 35.0 2.0 37.5 78-79 27.546271966930185 33.0 26.0 35.0 2.0 37.0 80-81 26.916583063695313 33.0 25.0 35.0 2.0 36.0 82-83 26.207846010875762 32.0 23.5 35.0 2.0 36.0 84-85 25.569186296227844 32.0 20.5 35.0 2.0 35.0 86-87 24.899031906225837 31.5 18.5 34.0 2.0 35.0 88-89 24.07853481779585 31.0 11.0 34.0 2.0 35.0 90-91 23.25300358967818 30.5 4.5 34.0 2.0 35.0 92-93 22.371122483776166 30.0 2.0 34.0 2.0 35.0 94-95 21.424231499100628 29.0 2.0 34.0 2.0 35.0 96-97 20.232025187618603 28.0 2.0 34.0 2.0 35.0 98-99 18.73562371634514 26.0 2.0 33.5 2.0 35.0 100-101 15.088266776344048 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4123308530430627 1101 2 0.2629600382605375 1101 3 0.2169252968977986 1101 4 0.21530331722286888 1101 5 0.18839139017468653 1101 6 0.3270519216680796 1101 7 0.2172109769578796 1101 8 0.36507735144844133 1101 9 0.3302158717441728 1101 10-11 0.43346850955045824 1101 12-13 0.4136385512518146 1101 14-15 0.5573776278307605 1101 16-17 0.6466187454025416 1101 18-19 0.4316308665701243 1101 20-21 0.6169923413605503 1101 22-23 0.6509902244658008 1101 24-25 0.6260778915704392 1101 26-27 0.6339777859268523 1101 28-29 0.7650402072307116 1101 30-31 0.7632148474327138 1101 32-33 0.7215111159976786 1101 34-35 0.8795215585972045 1101 36-37 0.900015817903828 1101 38-39 0.8899722565844215 1101 40-41 1.0190509578888438 1101 42-43 1.2252429051072014 1101 44-45 1.181404422169031 1101 46-47 1.2956397486747946 1101 48-49 1.373907814550428 1101 50-51 1.3061055205928511 1101 52-53 1.3487310540885176 1101 54-55 1.277691157632816 1101 56-57 1.3583295103926076 1101 58-59 1.3075809215008576 1101 60-61 1.2725593417814807 1101 62-63 1.571913768226267 1101 64-65 1.570342066013044 1101 66-67 1.6006582727725203 1101 68-69 1.7692181477903155 1101 70-71 2.027573131649337 1101 72-73 2.1868093234943338 1101 74-75 2.3908677900264834 1101 76-77 2.4756201907215534 1101 78-79 2.449488374604222 1101 80-81 2.6231803817806423 1101 82-83 2.811399216654987 1101 84-85 2.7824347466853645 1101 86-87 3.04813228393348 1101 88-89 3.3900962453812475 1101 90-91 3.592445136353618 1101 92-93 3.9608443969238643 1101 94-95 4.195811457569775 1101 96-97 4.672779749272806 1101 98-99 4.8722571766687715 1101 100-101 4.398429811589166 1102 1 0.27847135216592633 1102 2 0.2635432404026048 1102 3 0.19541285155109023 1102 4 0.08665348277514084 1102 5 0.1631594241523473 1102 6 0.25865423156930945 1102 7 0.018075495243429884 1102 8 0.16265244602887208 1102 9 0.2041308411056093 1102 10-11 0.3135117763706958 1102 12-13 0.3445799376834273 1102 14-15 0.4829863377619148 1102 16-17 0.495636885443524 1102 18-19 0.4155793671328709 1102 20-21 0.45568932285118535 1102 22-23 0.4624005919065226 1102 24-25 0.49130735648538604 1102 26-27 0.4875829452205096 1102 28-29 0.45039213290808533 1102 30-31 0.5718564656258209 1102 32-33 0.6284135295663091 1102 34-35 0.49594576978416427 1102 36-37 0.6123347067576574 1102 38-39 0.2812903752374254 1102 40-41 0.5144344279065223 1102 42-43 0.72187663663086 1102 44-45 0.7469062623529368 1102 46-47 0.7259058945501309 1102 48-49 0.9215089136351864 1102 50-51 0.9454575234071854 1102 52-53 0.9643090962356808 1102 54-55 1.0822354593738908 1102 56-57 1.104750823099991 1102 58-59 1.1473429189272863 1102 60-61 1.1102140978007995 1102 62-63 1.114860937592141 1102 64-65 1.1781672596443435 1102 66-67 1.28946236944617 1102 68-69 1.4633519991099995 1102 70-71 1.385051501161069 1102 72-73 1.7563366025115599 1102 74-75 1.722729677474316 1102 76-77 1.789689445683738 1102 78-79 1.9025499521017366 1102 80-81 1.873840428693935 1102 82-83 2.325559984097584 1102 84-85 2.3045862015935548 1102 86-87 2.459813390404687 1102 88-89 2.914083501421011 1102 90-91 2.9593459019963504 1102 92-93 3.4118738246728277 1102 94-95 3.577353635707059 1102 96-97 3.8066839545225584 1102 98-99 3.835888562527696 1102 100-101 3.782637889698515 1103 1 0.4348098371544573 1103 2 0.4911760245119865 1103 3 0.39130505432717655 1103 4 0.2657115057059585 1103 5 0.3344553221415012 1103 6 0.326877942177191 1103 7 0.43748796443666293 1103 8 0.45252308235561145 1103 9 0.5169227773275082 1103 10-11 0.5262570027971023 1103 12-13 0.5994406876744804 1103 14-15 0.79348580137966 1103 16-17 0.7560184896261646 1103 18-19 0.708248022994745 1103 20-21 0.7889551484116097 1103 22-23 0.6177580569845773 1103 24-25 0.7274533529572196 1103 26-27 0.872686365352088 1103 28-29 0.8803609397488046 1103 30-31 1.0210149279408753 1103 32-33 0.9570732820158128 1103 34-35 0.8948175397846754 1103 36-37 1.0220667897252227 1103 38-39 0.9815806079032185 1103 40-41 1.067273789607846 1103 42-43 1.0750504663544262 1103 44-45 1.1358551511014028 1103 46-47 1.4848028353059703 1103 48-49 1.3970694502153904 1103 50-51 1.4086245465199667 1103 52-53 1.4466663458471913 1103 54-55 1.4795854493619274 1103 56-57 1.4922633951537492 1103 58-59 1.588719270446866 1103 60-61 1.6165423804867984 1103 62-63 1.5031228839935054 1103 64-65 1.59805881830491 1103 66-67 1.7048350313867786 1103 68-69 1.9701966781904865 1103 70-71 2.042777385087799 1103 72-73 2.378280765462126 1103 74-75 2.3813760527256598 1103 76-77 2.35924326244886 1103 78-79 2.506021285535059 1103 80-81 2.6911398411479013 1103 82-83 2.758357645790756 1103 84-85 2.6750712068736284 1103 86-87 2.8900324962718464 1103 88-89 3.393687652083134 1103 90-91 3.571871398235583 1103 92-93 3.5676820183399407 1103 94-95 4.00324970622172 1103 96-97 4.464871035818923 1103 98-99 4.818821214387828 1103 100-101 4.901498711257959 1104 1 0.33111243482924735 1104 2 0.27487737204808127 1104 3 0.313691484210743 1104 4 0.20206715237709716 1104 5 0.29525401599489953 1104 6 0.3075006162199969 1104 7 0.24710934747965752 1104 8 0.2875315451852103 1104 9 0.4342364409665791 1104 10-11 0.36028920205843207 1104 12-13 0.25893869481460996 1104 14-15 0.4283369841503628 1104 16-17 0.5380325550074616 1104 18-19 0.42949932412177105 1104 20-21 0.3847503285615588 1104 22-23 0.4077409199747919 1104 24-25 0.4378500403890797 1104 26-27 0.4190359004688702 1104 28-29 0.45310340730208765 1104 30-31 0.4119872639108024 1104 32-33 0.48532680408521145 1104 34-35 0.43518620565173904 1104 36-37 0.5806778289184251 1104 38-39 0.7033414400609175 1104 40-41 0.8047413164713788 1104 42-43 0.7792963234426864 1104 44-45 0.7595553009841893 1104 46-47 0.784376963881094 1104 48-49 0.8789892884617458 1104 50-51 0.764728615547071 1104 52-53 0.8982707609608767 1104 54-55 1.099143656193128 1104 56-57 1.2497757635659923 1104 58-59 1.0921491381590087 1104 60-61 1.0569073477186564 1104 62-63 1.0957516801581946 1104 64-65 1.04487913684547 1104 66-67 1.0402219878855448 1104 68-69 1.2491477452467414 1104 70-71 1.6530985146862172 1104 72-73 1.5973997153315302 1104 74-75 1.5654916474045564 1104 76-77 1.7485318387737223 1104 78-79 2.103273727955237 1104 80-81 2.058687839745943 1104 82-83 2.2493472420583025 1104 84-85 2.36374968451538 1104 86-87 2.3692583190860823 1104 88-89 2.7710770144560364 1104 90-91 2.979592211160373 1104 92-93 3.248241287207822 1104 94-95 3.505893702015843 1104 96-97 3.510631255059927 1104 98-99 3.3286319323281433 1104 100-101 3.6378418530196033 1105 1 0.19732303104089155 1105 2 0.22848574557153256 1105 3 0.03301369411798305 1105 4 0.09049564392469023 1105 5 0.18646436257849075 1105 6 0.09936074462866884 1105 7 0.2334604343602038 1105 8 0.17024180689526247 1105 9 0.15939645254932344 1105 10-11 0.11970979038182605 1105 12-13 0.15020845287509843 1105 14-15 0.2545423132100666 1105 16-17 0.3347865166046873 1105 18-19 0.3033430469747245 1105 20-21 0.2584805954638796 1105 22-23 0.20614554708041055 1105 24-25 0.10386093415736752 1105 26-27 0.16583229883530493 1105 28-29 0.17511323765486253 1105 30-31 0.20999810276091324 1105 32-33 0.35716563922085953 1105 34-35 0.26513283517374475 1105 36-37 0.4248624937677974 1105 38-39 0.3266331104720024 1105 40-41 0.5031399525036946 1105 42-43 0.5017603520400726 1105 44-45 0.5173030069023667 1105 46-47 0.5558002931205337 1105 48-49 0.5052430453639616 1105 50-51 0.6820053731781996 1105 52-53 0.5814882668770593 1105 54-55 0.5327289890471789 1105 56-57 0.7269402884239611 1105 58-59 0.7257271069854596 1105 60-61 0.7347204062614239 1105 62-63 0.8336671860544129 1105 64-65 1.030067081807477 1105 66-67 1.164093045010432 1105 68-69 1.2955411191196724 1105 70-71 1.367128609844432 1105 72-73 1.2741167819023396 1105 74-75 1.3523328789695235 1105 76-77 1.229488213774971 1105 78-79 1.5138461001207126 1105 80-81 1.6969701740678005 1105 82-83 1.9085929659297314 1105 84-85 2.0609267656644654 1105 86-87 2.250177125065175 1105 88-89 2.410315798667561 1105 90-91 2.516497846763329 1105 92-93 2.8374971709410595 1105 94-95 3.253808434065089 1105 96-97 3.3122329934696957 1105 98-99 3.523279033147926 1105 100-101 3.531758417338766 1106 1 0.21808439645536382 1106 2 0.11856741286008088 1106 3 0.10400866689555244 1106 4 0.136272757023157 1106 5 0.15392031921518168 1106 6 0.05506060380224653 1106 7 0.12202851851903773 1106 8 0.09954874831387883 1106 9 0.15419570468478838 1106 10-11 0.05998519430490745 1106 12-13 0.2978058778780053 1106 14-15 0.32683095321198863 1106 16-17 0.09778011120914698 1106 18-19 0.21053953794939417 1106 20-21 0.179368293413269 1106 22-23 0.14595579250354973 1106 24-25 0.1653405945690949 1106 26-27 0.11939593953935201 1106 28-29 0.04165938940235492 1106 30-31 0.03427977767254475 1106 32-33 0.09936075603457795 1106 34-35 0.25708195711498405 1106 36-37 0.11450673653489929 1106 38-39 -0.22893941312946708 1106 40-41 0.314929551409179 1106 42-43 0.3583160025498202 1106 44-45 0.28897212097979974 1106 46-47 0.39648237847497825 1106 48-49 0.40971754414626815 1106 50-51 0.3645029759192937 1106 52-53 0.3180706382977263 1106 54-55 -0.033293176068788455 1106 56-57 0.37796860215794936 1106 58-59 0.49700152592600944 1106 60-61 0.5381050549777413 1106 62-63 0.28257447733240326 1106 64-65 0.515584980003208 1106 66-67 0.7909734889948226 1106 68-69 0.5017846244538227 1106 70-71 0.6826921362022382 1106 72-73 1.061063406280958 1106 74-75 1.2037171204527368 1106 76-77 1.3968259123261824 1106 78-79 1.6044216127730202 1106 80-81 1.7408267216940132 1106 82-83 1.8959546972521792 1106 84-85 2.0384965971140936 1106 86-87 2.009665606186008 1106 88-89 2.3092161689888933 1106 90-91 2.55270172209784 1106 92-93 2.8318638632854807 1106 94-95 2.9395711736405197 1106 96-97 2.867221749422722 1106 98-99 3.4379679612221317 1106 100-101 3.5340824545233627 1107 1 0.32103296698452866 1107 2 0.1711984932302002 1107 3 0.13346668197360145 1107 4 0.038915423561569185 1107 5 0.17142490631766805 1107 6 0.26472725735249725 1107 7 0.21361098507581744 1107 8 0.17701212071956007 1107 9 0.22812347403405653 1107 10-11 0.22835444475997235 1107 12-13 0.3115282004165252 1107 14-15 0.2783716221201047 1107 16-17 0.3290560616757219 1107 18-19 0.26600157831006754 1107 20-21 0.378576790519638 1107 22-23 0.26383303158522864 1107 24-25 0.2697840722850273 1107 26-27 0.3466302118624043 1107 28-29 0.3943838878087078 1107 30-31 0.42329193659106323 1107 32-33 0.40665545131835046 1107 34-35 0.40585528065472687 1107 36-37 0.5180810330212964 1107 38-39 0.6620573325417567 1107 40-41 0.46383768256752234 1107 42-43 0.5032454872349419 1107 44-45 0.4435130245260339 1107 46-47 0.6848151069957567 1107 48-49 0.8252532841963784 1107 50-51 0.8032478514080665 1107 52-53 0.728904871562996 1107 54-55 0.8754755858726142 1107 56-57 0.8285882071206174 1107 58-59 0.8021575165579762 1107 60-61 0.8911049981399302 1107 62-63 0.9912683299592615 1107 64-65 1.1423438550809983 1107 66-67 1.253872974977014 1107 68-69 1.2985676045911951 1107 70-71 1.4192029944093996 1107 72-73 1.5045812001579222 1107 74-75 1.6322370202433127 1107 76-77 1.6581133177968965 1107 78-79 1.9376084794504713 1107 80-81 2.206071131549983 1107 82-83 2.304867011090142 1107 84-85 2.276720969457113 1107 86-87 2.547169450024864 1107 88-89 2.901829002965659 1107 90-91 3.1907061029810997 1107 92-93 3.397713012831577 1107 94-95 3.830484484326174 1107 96-97 3.8730035388809085 1107 98-99 4.125596848235858 1107 100-101 3.9142078766169437 1108 1 0.19869965382731536 1108 2 0.008614280860037127 1108 3 0.017899246963629878 1108 4 0.04764044873586215 1108 5 -0.011195568041237891 1108 6 -0.0147058028695497 1108 7 -0.013360267622736899 1108 8 -0.05037572298521553 1108 9 0.10019220490113412 1108 10-11 0.0885322192973419 1108 12-13 0.08608093253819504 1108 14-15 0.12430849562868218 1108 16-17 0.19385783589027739 1108 18-19 0.2251056164686034 1108 20-21 0.18676082057105248 1108 22-23 0.10744907820306793 1108 24-25 0.19253475598033987 1108 26-27 0.09947321047392421 1108 28-29 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6770.0 12 8670.0 13 10581.0 14 12768.0 15 15847.0 16 19886.0 17 25316.0 18 24212.0 19 19393.0 20 19260.0 21 20010.0 22 21727.0 23 24126.0 24 27210.0 25 30298.0 26 34972.0 27 40142.0 28 46181.0 29 54422.0 30 64298.0 31 77589.0 32 95043.0 33 121044.0 34 160752.0 35 219912.0 36 276935.0 37 283261.0 38 126856.0 39 6354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07530247308459 17.5816485103485 14.354414608465325 22.98863440810159 2 16.148146373500232 19.397337669338302 39.235445798983044 25.21907015817842 3 19.513156859813822 23.550823342259626 29.287471539959693 27.64854825796686 4 13.16838429257844 15.90068052158041 35.144428516062895 35.786506669778255 5 13.99033952354155 36.97581233456304 33.09260301381855 15.941245128076867 6 33.40282387612377 34.87238993625489 16.253857063806596 15.470929123814745 7 29.603456906129434 33.04900808233071 19.02267188300023 18.324863128539633 8 28.02783340581616 33.20263854853789 18.695257018898488 20.074271026747464 9 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25.17944767461544 29.42884569609447 23.374501736792023 34-35 23.083989012560234 25.286529499900638 29.21641177844792 22.4130697090912 36-37 23.174113740681662 24.73536247924737 29.10678081968814 22.983742960382823 38-39 22.503867263487642 25.220390405489184 28.986991778045507 23.288750552977667 40-41 22.97057333285425 25.008370943997733 29.208892416054923 22.81216330709309 42-43 22.586002909898344 25.04639798535886 28.9442974220217 23.4233016827211 44-45 22.58711951884933 25.400114919216787 29.206091499544772 22.806674062389106 46-47 23.2356388237705 25.788835188458826 28.210822663334756 22.764703324435917 48-49 22.881310916737664 25.905301617919392 27.94342802268878 23.269959442654162 50-51 23.079684835842674 26.250514525299806 27.87072572996202 22.7990749088955 52-53 23.501650506138727 25.735529009412534 27.92061741088061 22.842203073568133 54-55 22.817697581483966 26.193799436539635 28.012014864189698 22.9764881177867 56-57 22.870034780521344 26.326764026438298 27.79744453380329 23.00575665923707 58-59 22.878271245014652 25.717526679852444 27.732258754019455 23.67194332111345 60-61 22.444180040198702 26.08903596013455 28.21100153088323 23.25578246878352 62-63 22.58509387761478 26.118818351404162 28.387710708804303 22.90837706217675 64-65 22.273581953311766 26.289117968216676 28.09341708492997 23.343882993541587 66-67 22.2648013868543 26.268278415322666 28.01035923306478 23.45656096475825 68-69 22.912677471717124 25.757301609823212 28.26376784805414 23.06625307040552 70-71 22.744037220346218 26.081293235196867 27.630795827030575 23.54387371742634 72-73 22.68734199954699 26.33410310416379 27.44494229322281 23.533612603066413 74-75 22.56287547994264 26.862781443988208 27.487240581044308 23.08710249502484 76-77 22.911566269325974 26.320861896426223 27.5178303469433 23.2497414873045 78-79 22.989117417339234 26.514319187711532 27.524811247071074 22.971752147878156 80-81 23.245542435998875 26.55435763770913 27.079426301235333 23.12067362505666 82-83 23.093339262412798 26.670521609724247 27.150636456132275 23.085502671730683 84-85 22.937850636270834 26.901321070029493 27.055888059862752 23.104940233836917 86-87 23.429780346134653 26.893964341096975 26.56880091986027 23.1074543929081 88-89 23.19464403490897 27.005238636709826 26.429610912945517 23.370506415435685 90-91 23.10999062335059 26.829065061344014 26.514800185710257 23.546144129595138 92-93 23.351828882841733 26.38341711525664 26.766996691421124 23.497757310480498 94-95 23.2630401695681 26.132261554625952 26.977940783966027 23.626757491839925 96-97 23.653931177637393 25.721523509368698 26.805950391532058 23.81859492146185 98-99 23.293325147355414 26.205830478395427 26.379704398713365 24.121139975535797 100-101 24.214063319166065 25.033232642575793 26.55488228514403 24.19782175311412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1950.0 1 1336.0 2 980.0 3 1245.0 4 987.5 5 912.0 6 1784.0 7 3842.0 8 6255.5 9 6908.0 10 5178.0 11 3419.5 12 3014.0 13 3163.0 14 3340.0 15 3389.0 16 3459.0 17 3562.0 18 3776.0 19 4042.5 20 4263.0 21 4782.5 22 5617.0 23 6541.5 24 7580.5 25 9048.5 26 10922.5 27 12820.0 28 14552.0 29 16239.5 30 17990.0 31 21403.0 32 27422.5 33 28767.5 34 26768.0 35 27446.5 36 29301.0 37 32259.5 38 35491.5 39 37998.5 40 40749.5 41 45002.5 42 47879.5 43 50407.0 44 54006.5 45 58733.5 46 62496.0 47 66451.0 48 68744.5 49 68068.0 50 68826.0 51 69506.5 52 70825.0 53 74151.0 54 77988.5 55 75878.5 56 71343.0 57 67486.5 58 63567.5 59 57599.0 60 47430.5 61 39528.5 62 33371.0 63 25274.0 64 18464.0 65 14462.5 66 10990.0 67 8422.5 68 6604.0 69 5081.5 70 3870.5 71 2670.0 72 1826.5 73 1375.5 74 956.0 75 650.5 76 486.5 77 311.5 78 179.0 79 119.5 80 67.0 81 41.0 82 29.0 83 22.0 84 18.0 85 11.0 86 7.5 87 5.0 88 4.0 89 3.0 90 1.5 91 1.0 92 1.0 93 2.5 94 2.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4665800701275781 2 0.038421791090621805 3 0.051437302977688816 4 0.05627907339967774 5 0.08460082726593554 6 0.08220597307871522 7 0.04779295964931005 8 0.04112901756313174 9 0.046179036175313735 10-11 0.04979734847991836 12-13 0.04513779522434838 14-15 0.039463032041587166 16-17 0.03935890794649063 18-19 0.046933935864763625 20-21 0.056305104423451866 22-23 0.046413315389280944 24-25 0.06523374557797984 26-27 0.056096856233258795 28-29 0.04417464734470542 30-31 0.045632384676056924 32-33 0.04508573317680011 34-35 0.04979734847991836 36-37 0.04412258529715715 38-39 0.026004992750359876 40-41 0.023532045491817147 42-43 0.023063487063882736 44-45 0.01590495552599588 46-47 0.0293890258409973 48-49 0.028165567723613005 50-51 0.016946196476961245 52-53 0.016321451906382026 54-55 0.01720650671470258 56-57 0.016321451906382026 58-59 0.014655466384837449 60-61 0.01692016545318711 62-63 0.017310630809799117 64-65 0.0179093443566042 66-67 0.00934513753491411 68-69 0.012104426054972316 70-71 0.017180475690928447 72-73 0.01561861426448041 74-75 0.012651077554229131 76-77 0.009527354701333049 78-79 0.014837683551256387 80-81 0.019236926569085037 82-83 0.015983048597318285 84-85 0.013458039291227283 86-87 0.0030456297815736793 88-89 0.02777510236700099 90-91 0.030690577029704003 92-93 0.016816041358090575 94-95 0.03188800412331416 96-97 0.026447520154520156 98-99 0.022282556350658716 100-101 0.1492358592971103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1920785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.61223596149763 #Duplication Level Percentage of deduplicated Percentage of total 1 73.83535189363457 34.416308447663056 2 13.509108751449302 12.593795295041751 3 4.988500629643829 6.97575505329113 4 2.3474187192700144 4.376737409722019 5 1.3396950552103417 3.1223091014958024 6 0.858876586806713 2.402049487562413 7 0.5377673590359399 1.7546577322242254 8 0.40293857238698866 1.502549425127305 9 0.2901507282728994 1.2172116789591094 >10 1.6800786383390511 14.342687950781999 >50 0.13122219939695604 4.197784409426108 >100 0.06845531708089098 5.943898711767029 >500 0.005161669631605823 1.6024280760934606 >1k 0.004937249212840353 3.8583907283787084 >5k 1.1221020938273531E-4 0.38113697132529445 >10k+ 2.2442041876547062E-4 1.3122995211406119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14099 0.7340228083830309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10991 0.5722139646030139 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7287 0.3793761404842291 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3327 0.17321043219308774 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3311 0.17237743943231543 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3028 0.1576438799761556 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2950 0.15358304026739067 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2639 0.1373917434798793 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2571 0.13385152424659708 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2490 0.12963449839518737 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2378 0.12380354906978137 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2284 0.11890971660024417 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2122 0.11047566489742475 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2121 0.11042360284987647 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.206204754826803E-5 2 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 3 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 4 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 5 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 6 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 7 1.0412409509653605E-4 0.0 0.0 0.0 5.206204754826803E-5 8 1.561861426448041E-4 0.0 0.0 0.0 5.206204754826803E-5 9 1.561861426448041E-4 0.0 0.0 0.0 5.206204754826803E-5 10-11 1.561861426448041E-4 0.0 0.0 0.0 5.206204754826803E-5 12-13 1.561861426448041E-4 0.0 0.0 0.0 1.561861426448041E-4 14-15 2.082481901930721E-4 0.0 0.0 0.0 1.561861426448041E-4 16-17 2.342792139672061E-4 0.0 0.0 2.6031023774134014E-5 2.8634126151547417E-4 18-19 2.8634126151547417E-4 0.0 0.0 1.0412409509653605E-4 3.384033090637422E-4 20-21 3.123722852896082E-4 0.0 0.0 1.0412409509653605E-4 3.644343328378762E-4 22-23 3.644343328378762E-4 0.0 0.0 2.3427921396720615E-4 4.164963803861442E-4 24-25 3.644343328378762E-4 0.0 0.0 6.247445705792164E-4 5.466514992568143E-4 26-27 3.644343328378762E-4 0.0 0.0 0.001770109616641113 6.247445705792164E-4 28-29 5.726825230309483E-4 0.0 0.0 0.005778887277857751 6.247445705792164E-4 30-31 6.768066181274844E-4 0.0 0.0 0.01926295759285917 6.247445705792164E-4 32-33 7.028376419016184E-4 0.0 0.0 0.04336768560770726 7.288686656757524E-4 34-35 7.288686656757524E-4 0.0 0.0 0.07640105477708332 7.288686656757524E-4 36-37 8.329927607722884E-4 0.0 0.0 0.13012908784689595 7.288686656757524E-4 38-39 9.371168558688245E-4 0.0 0.0 0.21293377447241624 7.548996894498864E-4 40-41 9.371168558688245E-4 0.0 0.0 0.3016214724708908 7.809307132240204E-4 42-43 9.371168558688245E-4 0.0 0.0 0.39025710842181716 7.809307132240204E-4 44-45 0.0011453650460618967 0.0 0.0 0.49534435139799615 8.069617369981545E-4 46-47 0.0013536132362549688 0.0 0.0 0.6160241776148814 8.850548083205565E-4 48-49 0.0014577373313515048 0.0 0.0 0.741623867325078 8.850548083205565E-4 50-51 0.0014577373313515048 0.0 0.0 0.8826599541333362 9.371168558688245E-4 52-53 0.0014577373313515048 0.0 0.0 1.010862746220946 0.0010412409509653604 54-55 0.0015097993788997727 0.0 0.0 1.142501633446742 0.0011193340222877626 56-57 0.0016659855215445769 0.0 0.0 1.2976465351405806 0.0011453650460618967 58-59 0.0016659855215445769 0.0 0.0 1.4600801234911769 0.0011453650460618967 60-61 0.0016659855215445769 0.0 0.0 1.6355292237288401 0.0011453650460618967 62-63 0.0016659855215445769 0.0 0.0 1.8063448017347075 0.0011453650460618967 64-65 0.0016659855215445769 0.0 0.0 1.9794511098326986 0.0011453650460618967 66-67 0.001770109616641113 0.0 0.0 2.1792652483229515 0.0011713960698360305 68-69 0.001770109616641113 0.0 0.0 2.4020908118295385 0.0011974270936101646 70-71 0.0018221716641893808 0.0 0.0 2.627155043380701 0.0012494891411584327 72-73 0.0018221716641893808 0.0 0.0 2.8535468571443445 0.0013536132362549688 74-75 0.0018221716641893808 0.0 0.0 3.0821773389525635 0.0013536132362549688 76-77 0.0018221716641893808 0.0 0.0 3.32351096036256 0.0014056752838032367 78-79 0.0018221716641893808 0.0 0.0 3.5992315641781873 0.0014056752838032367 80-81 0.001874233711737649 0.0 0.0 3.878023828799163 0.0014317063075773707 82-83 0.001978357806834185 0.0 0.0 4.164078749053122 0.0014577373313515048 84-85 0.002030419854382453 0.0 0.0 4.459687055032187 0.0014577373313515048 86-87 0.002134543949478989 0.0 0.0 4.760839969075144 0.0014837683551256389 88-89 0.002186605997027257 0.0 0.0 5.076934690764453 0.0015097993788997727 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5370 0.0 54.13777 1 GTATCAA 9700 0.0 43.258804 1 TATCAAC 12330 0.0 33.920322 2 ATCAACG 12545 0.0 33.30197 3 TCAACGC 12660 0.0 32.924377 4 CAACGCA 12900 0.0 32.27499 5 AACGCAG 13155 0.0 31.791395 6 GTGTAAG 630 0.0 31.067097 1 GTGGTAT 2515 0.0 29.04095 1 TAAGGTG 1210 0.0 27.88847 5 ACGCAGA 14885 0.0 27.861347 7 CGCAGAG 15015 0.0 27.556847 8 TGGTATC 2535 0.0 27.185066 2 TAAGGTA 755 0.0 27.069063 4 AGGTAAG 1135 0.0 26.380625 2 GTACATG 8975 0.0 25.424406 1 TACATGG 8780 0.0 25.279165 2 GCAGAGT 16360 0.0 24.766075 9 TGTAAGG 820 0.0 24.343098 2 TATACCG 100 0.0016483363 23.764118 5 >>END_MODULE