FastQCFastQC Report
Thu 26 May 2016
SRR938026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920785
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142500.7418841775628193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114920.5982970504246962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75760.39442207222567854No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33790.17591765866559764No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33360.17367899062102213No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31010.16144440944717917No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29890.15561346012177313No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29380.15295829569681146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.1389015428587791No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24980.13005099477557353No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.12541747254377766No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23330.1214607569301093No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA21490.11188134018122799No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA20620.10735194204452866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.057.8741841
GTATCAA97900.043.5742721
TATCAAC124600.034.1225472
ATCAACG127700.033.326193
TCAACGC129000.033.172724
CAACGCA131900.032.479385
AACGCAG135250.031.7100186
GTGGTAT22700.030.5536771
TGGTATC22800.029.7946052
ACGCAGA153600.027.8289817
CGCAGAG153800.027.5766268
GCAGAGT167850.025.155129
TAAGGTA5600.024.595524
GAGTACT107900.023.32923112-13
CAGAGTA158800.022.59591710-11
GTACTTT111100.022.48628214-15
TACATGG88200.022.4596982
TAAGGTG11550.022.2053595
AGGTAAG10300.022.1381052
GTACATG90800.022.1304871
Produced by FastQC (version 0.11.5)