##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1920785 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.511609576293026 33.0 31.0 34.0 31.0 34.0 2 32.739703819011496 34.0 31.0 34.0 31.0 34.0 3 32.68977683603318 34.0 31.0 34.0 31.0 34.0 4 36.224179176742844 37.0 35.0 37.0 35.0 37.0 5 36.18934446072829 37.0 35.0 37.0 35.0 37.0 6 36.25254518334952 37.0 37.0 37.0 35.0 37.0 7 36.21498033356154 37.0 37.0 37.0 35.0 37.0 8 36.20914313679042 37.0 37.0 37.0 35.0 37.0 9 37.91294340595121 39.0 38.0 39.0 35.0 39.0 10-11 37.88441704823809 39.0 38.0 39.0 35.0 39.0 12-13 37.82473155506733 39.0 38.0 39.0 35.0 39.0 14-15 39.09297552823455 40.0 38.5 41.0 36.0 41.0 16-17 39.030426882758874 40.0 38.0 41.0 36.0 41.0 18-19 39.0453299041798 40.0 38.5 41.0 36.0 41.0 20-21 39.04903672196524 40.0 38.5 41.0 35.5 41.0 22-23 39.07092699078762 40.0 39.0 41.0 35.0 41.0 24-25 39.00844550535328 40.0 38.5 41.0 35.0 41.0 26-27 38.82353777231705 40.0 38.0 41.0 35.0 41.0 28-29 38.73907699195902 40.0 38.0 41.0 35.0 41.0 30-31 38.49244007007552 40.0 38.0 41.0 34.5 41.0 32-33 38.251344632533055 40.0 38.0 41.0 34.0 41.0 34-35 38.02851230096029 40.0 37.0 41.0 33.5 41.0 36-37 37.92445146125152 40.0 37.0 41.0 33.0 41.0 38-39 37.852653993028895 40.0 37.0 41.0 33.0 41.0 40-41 37.70872794196123 40.0 37.0 41.0 33.0 41.0 42-43 37.489783604099365 40.0 37.0 41.0 33.0 41.0 44-45 37.202805103121904 40.0 36.0 41.0 32.0 41.0 46-47 36.78058371967711 40.0 36.0 41.0 31.0 41.0 48-49 36.333329602219926 39.0 35.0 41.0 30.0 41.0 50-51 35.9843267205856 39.0 35.0 41.0 29.5 41.0 52-53 35.491479264988016 38.5 35.0 40.0 28.0 41.0 54-55 35.148895894126625 38.0 35.0 40.0 27.0 41.0 56-57 34.83905903055261 38.0 34.0 40.0 26.0 41.0 58-59 34.42696918187096 37.5 34.0 40.0 26.0 41.0 60-61 33.983330773616 37.0 33.5 40.0 25.0 41.0 62-63 33.526393896245544 36.0 33.0 39.0 23.5 41.0 64-65 33.06448613457519 35.5 33.0 39.0 22.5 40.0 66-67 32.54156425628064 35.0 32.0 38.5 21.5 40.0 68-69 31.99549429009494 35.0 31.5 37.5 19.5 40.0 70-71 31.405899931538407 35.0 31.0 37.0 17.5 39.0 72-73 30.953094177640914 34.5 30.5 36.5 15.0 39.0 74-75 30.42456079155137 34.0 30.0 36.0 10.0 38.5 76-77 29.24232227969294 32.5 28.5 35.0 9.0 36.5 78-79 29.94145466567055 34.0 30.0 35.0 7.5 37.0 80-81 29.788124907264475 34.0 30.0 35.0 6.0 37.0 82-83 29.35828528440195 34.0 29.5 35.0 2.0 36.0 84-85 28.895286302214977 34.0 29.0 35.0 2.0 36.0 86-87 28.394579299609276 34.0 28.5 35.0 2.0 35.0 88-89 27.784509198062253 33.0 27.0 35.0 2.0 35.0 90-91 27.280827630369878 33.0 26.0 35.0 2.0 35.0 92-93 26.671820896144027 33.0 25.0 35.0 2.0 35.0 94-95 25.94676994041499 32.0 23.5 34.0 2.0 35.0 96-97 25.139201940873132 32.0 19.5 34.0 2.0 35.0 98-99 24.1285841465859 31.5 8.5 34.0 2.0 35.0 100-101 20.644965730157203 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.00749418835611948 1101 2 -0.0031921304153712526 1101 3 0.10012076514944823 1101 4 0.06425110847958138 1101 5 -0.032141264485247234 1101 6 -0.0410872053377318 1101 7 -0.01589678543992079 1101 8 0.09911143235773068 1101 9 -0.03369541270619436 1101 10-11 0.06552807545148198 1101 12-13 0.09359188439359656 1101 14-15 0.19473065162242165 1101 16-17 0.16205672427420126 1101 18-19 0.19090466541557305 1101 20-21 0.2512327808930266 1101 22-23 0.16258353887970856 1101 24-25 0.04495690685753573 1101 26-27 0.212823375563282 1101 28-29 0.23353019396351726 1101 30-31 0.20326418574989447 1101 32-33 0.20078255112735377 1101 34-35 0.16153526609374325 1101 36-37 0.16573463006788813 1101 38-39 0.2490531383369543 1101 40-41 0.23824141363196105 1101 42-43 0.18070718904683503 1101 44-45 0.040409148822845964 1101 46-47 0.031725188159065 1101 48-49 0.07044864078304869 1101 50-51 0.10756310287751347 1101 52-53 0.02725415607222459 1101 54-55 0.13787091107160165 1101 56-57 0.10068823835982954 1101 58-59 0.08474014013775388 1101 60-61 0.3472752302693749 1101 62-63 0.3624780025591363 1101 64-65 0.2571216509081893 1101 66-67 0.34403872750176134 1101 68-69 0.43896412921699834 1101 70-71 0.36197668363141844 1101 72-73 0.5580758841821414 1101 74-75 0.6232406314401508 1101 76-77 0.593298099920446 1101 78-79 0.6628326800644295 1101 80-81 0.7569199477448976 1101 82-83 0.8323421329956737 1101 84-85 1.0683702061256035 1101 86-87 1.3355068625821858 1101 88-89 1.4753968648481681 1101 90-91 1.5018207528208336 1101 92-93 1.7630898337508079 1101 94-95 1.8522920501667706 1101 96-97 2.1573441226110894 1101 98-99 2.2857238870953687 1101 100-101 2.8500788224566342 1102 1 0.07697822392794507 1102 2 0.11545093701752052 1102 3 0.22875073098712306 1102 4 0.08284812748682668 1102 5 0.0579494415633377 1102 6 0.05033469758424047 1102 7 0.11231243336139585 1102 8 0.10860921233274468 1102 9 0.11861618319900202 1102 10-11 0.11954264498635325 1102 12-13 0.19567855594560513 1102 14-15 0.31684838833061946 1102 16-17 0.2748131316087523 1102 18-19 0.22587780188604256 1102 20-21 0.2997525110071777 1102 22-23 0.3316350231233116 1102 24-25 0.21105848687953 1102 26-27 0.2528881355446302 1102 28-29 0.17230273460943835 1102 30-31 0.17008185385294894 1102 32-33 0.14564686605183397 1102 34-35 0.028890380098665958 1102 36-37 0.25557288789465815 1102 38-39 0.29907440943711805 1102 40-41 0.2618803846249591 1102 42-43 0.2418583880005798 1102 44-45 0.33293001962895374 1102 46-47 0.2893094456749523 1102 48-49 0.3958640408258205 1102 50-51 0.3663616830900125 1102 52-53 0.3902746990462802 1102 54-55 0.41699274188220414 1102 56-57 0.5123632089367547 1102 58-59 0.4821032833986152 1102 60-61 0.4835885044877557 1102 62-63 0.43134727996116595 1102 64-65 0.4408405521506822 1102 66-67 0.5014533421500644 1102 68-69 0.4224486392979756 1102 70-71 0.6918990441157895 1102 72-73 0.7691369147085076 1102 74-75 0.6922156799721542 1102 76-77 0.8309699195695686 1102 78-79 0.8707909933830607 1102 80-81 1.0469753577921033 1102 82-83 0.8938600246790251 1102 84-85 0.8618494451812175 1102 86-87 0.9849620536071839 1102 88-89 1.1818708761218524 1102 90-91 1.331339313024472 1102 92-93 1.4792477471260028 1102 94-95 1.6767216165285141 1102 96-97 1.7989167347839974 1102 98-99 2.0600305018231495 1102 100-101 2.5291099004689777 1103 1 0.20841699983436968 1103 2 0.22735154222723963 1103 3 0.3457589590679717 1103 4 0.2234457540048993 1103 5 0.08552459027712445 1103 6 0.1220051114398828 1103 7 0.09176202675395473 1103 8 0.11489504887206436 1103 9 0.2004108595766425 1103 10-11 0.24151155232736699 1103 12-13 0.1831746444284974 1103 14-15 0.42268623719797205 1103 16-17 0.3305765188950076 1103 18-19 0.3300505436177943 1103 20-21 0.41273653983554226 1103 22-23 0.3097862973832832 1103 24-25 0.3440376962619922 1103 26-27 0.4333139522427558 1103 28-29 0.3249603963482883 1103 30-31 0.3181863574081447 1103 32-33 0.5390744894711474 1103 34-35 0.5074400390448304 1103 36-37 0.5071483442002602 1103 38-39 0.6076023161606727 1103 40-41 0.4722347319098148 1103 42-43 0.3831829285733832 1103 44-45 0.3667628343452449 1103 46-47 0.4001134937049571 1103 48-49 0.48001057634468225 1103 50-51 0.43643513205451967 1103 52-53 0.46563983107854057 1103 54-55 0.5315449940404875 1103 56-57 0.4706645331803969 1103 58-59 0.514637207957918 1103 60-61 0.5049865016387258 1103 62-63 0.4368093186400728 1103 64-65 0.32142545624522967 1103 66-67 0.49255069115027794 1103 68-69 0.6618951567724665 1103 70-71 0.8674060566760424 1103 72-73 0.6310856763088672 1103 74-75 0.7217422093848569 1103 76-77 0.3348545985282314 1103 78-79 0.6062656555079258 1103 80-81 0.8298842550374061 1103 82-83 0.8029354295924236 1103 84-85 0.8306429619986453 1103 86-87 0.5966133114977907 1103 88-89 1.006589037514665 1103 90-91 1.0327004067722143 1103 92-93 1.42791379607117 1103 94-95 1.4841523950479463 1103 96-97 1.7745130555596198 1103 98-99 2.1142968534873816 1103 100-101 2.4917057088453767 1104 1 0.0680379425636417 1104 2 0.03539624992058066 1104 3 0.0676909851979488 1104 4 -0.0029508770668087436 1104 5 -0.014118051112006924 1104 6 0.013496665342209724 1104 7 0.026455048838329276 1104 8 0.03763654773517544 1104 9 0.07140633843541622 1104 10-11 0.09239297232148402 1104 12-13 0.008959591687720092 1104 14-15 0.04595012425221512 1104 16-17 0.12243177192568311 1104 18-19 0.18548375066915668 1104 20-21 0.1308063101855126 1104 22-23 0.14630943939150143 1104 24-25 0.04270653983966355 1104 26-27 0.06340005999280862 1104 28-29 0.015463181772844337 1104 30-31 0.031954438890842596 1104 32-33 0.10300980210269017 1104 34-35 0.04431138113864108 1104 36-37 0.0735892179940123 1104 38-39 0.0499092429999024 1104 40-41 0.005353368275848425 1104 42-43 -0.0691236988525219 1104 44-45 -0.05214018567846068 1104 46-47 -0.17232093037350893 1104 48-49 -0.12589050805993907 1104 50-51 -0.019043867623175004 1104 52-53 -0.008533127671107366 1104 54-55 -0.10188503218459033 1104 56-57 0.0802163466564636 1104 58-59 0.07192290286982939 1104 60-61 0.17337567555735234 1104 62-63 0.12377444755512101 1104 64-65 -0.03466769300174377 1104 66-67 0.10673795128938224 1104 68-69 0.14402370371959705 1104 70-71 0.12618899434347242 1104 72-73 0.2660501077669899 1104 74-75 0.2576867791719408 1104 76-77 0.19413473844231888 1104 78-79 0.2983190497102264 1104 80-81 0.27807518210912363 1104 82-83 0.25037183508260696 1104 84-85 0.5842513328907408 1104 86-87 0.5919546162706126 1104 88-89 0.810027535375621 1104 90-91 0.8184205046275324 1104 92-93 0.920146309892619 1104 94-95 1.0165793866309087 1104 96-97 1.1020578486738195 1104 98-99 1.4978058719928882 1104 100-101 1.6937640873989288 1105 1 0.06718047019747075 1105 2 0.005378226775064832 1105 3 0.1119832397386844 1105 4 0.031953115983547775 1105 5 0.05592233870716257 1105 6 0.03910550816640068 1105 7 0.07462677296937414 1105 8 0.06237918121129127 1105 9 0.050981521412779784 1105 10-11 -0.003911533964149783 1105 12-13 0.011694017393423906 1105 14-15 0.013821870350533061 1105 16-17 0.07503963620791154 1105 18-19 0.07254665194625431 1105 20-21 0.025424578965036915 1105 22-23 0.02795753373094101 1105 24-25 0.047196599650547455 1105 26-27 0.04482224094319065 1105 28-29 -0.030912842950314712 1105 30-31 0.012975717186179736 1105 32-33 -0.019138197378083532 1105 34-35 0.019591713443510628 1105 36-37 0.05283751303440454 1105 38-39 0.13717272041854756 1105 40-41 0.14634766177853464 1105 42-43 0.10492858823970863 1105 44-45 0.16049111867805266 1105 46-47 0.10386892174683027 1105 48-49 0.22163193350213817 1105 50-51 0.39800785031710006 1105 52-53 0.3436801994714429 1105 54-55 0.4488849455485848 1105 56-57 0.37710710442738815 1105 58-59 0.2522585777143149 1105 60-61 0.3942322242997278 1105 62-63 0.4723862659457225 1105 64-65 0.5826106872117336 1105 66-67 0.5704171942174199 1105 68-69 0.7399532686678256 1105 70-71 0.9149119221809947 1105 72-73 1.000165400980105 1105 74-75 0.6885728838759313 1105 76-77 0.6280157557265653 1105 78-79 0.8338223997187129 1105 80-81 0.9003392586350998 1105 82-83 0.8843495832378565 1105 84-85 0.8761732442428247 1105 86-87 1.060623924002094 1105 88-89 1.1582119905374313 1105 90-91 1.3939488424042423 1105 92-93 1.516125426009605 1105 94-95 1.5113349508402365 1105 96-97 1.572880198372765 1105 98-99 1.6687554071854507 1105 100-101 1.9930898420245349 1106 1 -0.0017778355523390132 1106 2 0.058615930542075034 1106 3 0.14219522161982212 1106 4 0.07510377618262964 1106 5 0.04108491097598943 1106 6 0.005680296625854453 1106 7 0.02205659045672803 1106 8 -0.008538557702138405 1106 9 0.051269894606811306 1106 10-11 0.08580512808854479 1106 12-13 0.04905113474476508 1106 14-15 0.02374165746591217 1106 16-17 0.033558531422620774 1106 18-19 0.0030915920248162365 1106 20-21 0.0028126099227776535 1106 22-23 0.025051233453694977 1106 24-25 0.02769203984344415 1106 26-27 0.01590367552710603 1106 28-29 -0.06283074034361391 1106 30-31 0.07219703934969601 1106 32-33 0.23760421865767256 1106 34-35 0.13257282023486994 1106 36-37 0.2169010474165134 1106 38-39 0.3249101894748492 1106 40-41 0.40011481872977583 1106 42-43 0.29382180690583226 1106 44-45 0.35944580346357213 1106 46-47 0.05928868278325439 1106 48-49 0.1945283638933617 1106 50-51 0.30406564331429564 1106 52-53 0.22501713772046372 1106 54-55 0.3308357425619377 1106 56-57 0.40458656222559597 1106 58-59 0.4522960411536019 1106 60-61 0.6106357441578609 1106 62-63 0.5345881131390158 1106 64-65 0.47129888507486584 1106 66-67 0.4135544638699358 1106 68-69 0.12906750658022048 1106 70-71 0.5464655164644832 1106 72-73 0.5935955574940301 1106 74-75 0.46920689365754953 1106 76-77 0.6339597541538033 1106 78-79 0.8220963933714955 1106 80-81 0.8907075939544455 1106 82-83 0.9643796643233813 1106 84-85 0.9605735583940778 1106 86-87 1.1145825607541795 1106 88-89 1.313265994060096 1106 90-91 1.1559970744508803 1106 92-93 1.1796538400473082 1106 94-95 1.4791550099793085 1106 96-97 1.669348053036689 1106 98-99 1.8317434349334718 1106 100-101 2.156545285106187 1107 1 0.10598737475483233 1107 2 0.0977902958167931 1107 3 0.23152749398075656 1107 4 0.12899476126926146 1107 5 0.08083931335559669 1107 6 0.07212442141835851 1107 7 0.07632139342628363 1107 8 0.030448534286207973 1107 9 0.062203156820018535 1107 10-11 0.06886345387994197 1107 12-13 0.09238668329518163 1107 14-15 0.2123364228496527 1107 16-17 0.2336327120473385 1107 18-19 0.16417831631627422 1107 20-21 0.17753129921380406 1107 22-23 0.10993521874582513 1107 24-25 0.08665069835461736 1107 26-27 0.05793669100594201 1107 28-29 0.12871048236877414 1107 30-31 0.1254635372517967 1107 32-33 0.1463580424575568 1107 34-35 0.07213455702336091 1107 36-37 0.17078336109416625 1107 38-39 0.1636817350114086 1107 40-41 0.15547360374150543 1107 42-43 0.18074699815047524 1107 44-45 0.17809712975193293 1107 46-47 0.272703352475439 1107 48-49 0.4044762917180904 1107 50-51 0.4658278630784025 1107 52-53 0.36651084985592064 1107 54-55 0.5154216208445064 1107 56-57 0.4354695302667224 1107 58-59 0.4953113305236201 1107 60-61 0.5119572960340122 1107 62-63 0.5056245141107993 1107 64-65 0.5630023141434677 1107 66-67 0.7012423018909217 1107 68-69 0.6779163223863804 1107 70-71 0.8403876603282647 1107 72-73 0.7308938379317702 1107 74-75 0.6963340485755509 1107 76-77 0.8513457834209035 1107 78-79 0.9634152199476844 1107 80-81 1.0943017108042348 1107 82-83 1.1513192107576842 1107 84-85 1.2726619397262091 1107 86-87 1.2079834727823702 1107 88-89 1.228950022490661 1107 90-91 1.2921134199839415 1107 92-93 1.5264446171658648 1107 94-95 1.6209281746646695 1107 96-97 1.636533328255549 1107 98-99 1.7976611541925962 1107 100-101 2.112373200630632 1108 1 0.03821647825648711 1108 2 0.03484401381701474 1108 3 0.09651280209761381 1108 4 -0.037372244172786395 1108 5 -0.002130620558439489 1108 6 0.04106534940979145 1108 7 -3.641379118164423E-4 1108 8 -0.03404122083072991 1108 9 -0.04936803094811637 1108 10-11 0.030135737027926268 1108 12-13 -0.08838286140318274 1108 14-15 0.08355340050368198 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-2.9551803694079872 2316 100-101 -3.9208874570343895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 10.0 5 24.0 6 68.0 7 181.0 8 490.0 9 861.0 10 1545.0 11 2596.0 12 3917.0 13 5709.0 14 7842.0 15 10749.0 16 14480.0 17 21563.0 18 21705.0 19 15463.0 20 13301.0 21 13232.0 22 13537.0 23 14908.0 24 16630.0 25 19087.0 26 22270.0 27 26527.0 28 32528.0 29 39558.0 30 47909.0 31 58627.0 32 74274.0 33 98705.0 34 139322.0 35 212233.0 36 320175.0 37 412356.0 38 225933.0 39 12460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.408926558672626 18.009615860182166 14.730852229687342 22.85060535145787 2 16.370270747323843 19.72291602558195 38.78108252068523 25.125730706408973 3 19.888111378380348 23.478023179532038 29.370268553518063 27.263596888569552 4 13.442004180582417 16.245493379009105 34.94878396072439 35.36371847968409 5 14.001030831224814 37.07660429617135 33.10087568591926 15.821489186684575 6 33.48323755637027 35.06358347797928 16.05012958263874 15.40304938301171 7 29.724721923588532 33.05783833172375 18.87244017419961 18.344999570488106 8 28.04725989349135 33.441466318683574 18.502246219380325 20.009027568444743 9 27.26078139927165 16.096387674830865 19.1328024739885 37.51002845190898 10-11 25.91344163974625 26.014546136084988 25.529588163172868 22.542424060995895 12-13 27.145203653714496 23.315181032754836 26.521630479205115 23.01798483432555 14-15 23.49094250527779 24.397394815140686 25.269069677241333 26.842593002340188 16-17 22.59422579830642 27.830184013307058 26.25673878127953 23.31885140710699 18-19 22.805441525209744 25.60887866158888 29.230913402593213 22.354766410608164 20-21 23.031130501331486 24.537311567926658 30.044695267820188 22.38686266292167 22-23 23.11055115486637 24.517111493477927 29.936171929705825 22.43616542194988 24-25 22.940195387574573 24.81837139882853 29.769545330422314 22.47188788317459 26-27 22.017574190914413 25.300966090997523 29.802673101718675 22.87878661636939 28-29 22.436950027540924 25.723092413594905 29.519236476052935 22.320721082811236 30-31 22.315436547344635 25.205821065112787 29.367614500860977 23.1111278866816 32-33 22.026669332350362 25.163455350839374 29.4915728991601 23.31830241765016 34-35 22.98727608304041 25.36243675598047 29.27451204503555 22.375775115943572 36-37 23.16966783287328 24.745283345512593 29.14793676711282 22.937112054501306 38-39 22.46205456061298 25.210055808319748 29.08729416874141 23.24059546232587 40-41 22.953495596086093 24.98603719612838 29.2599961307736 22.800471077011927 42-43 22.59967277377858 25.110915316936612 28.99388872225555 23.29552318702926 44-45 22.563073088477143 25.360602675404355 29.32731671517225 22.74900752094626 46-47 23.232610632749797 25.781061473586554 28.249959455635615 22.736368438028038 48-49 22.832286883453598 25.89658888576516 28.009714803361135 23.26140942742011 50-51 23.053048623349305 26.165552104998735 27.996314007033586 22.78508526461837 52-53 23.508799787586845 25.707380055550207 27.947063310052922 22.836756846810026 54-55 22.801302592429657 26.145768526930397 28.025416691613064 23.027512189026883 56-57 22.787037591401432 26.320566851573705 27.783614511775134 23.108781045249728 58-59 22.827567895417758 25.762487732880047 27.739257647264008 23.670686724438188 60-61 22.380589186192104 26.231514719242394 28.10002160574973 23.28787448881577 62-63 22.56738781279529 26.239662429683698 28.279895980028996 22.913053777492014 64-65 22.234711328961858 26.366225267273535 28.02218884466507 23.376874559099534 66-67 22.162660130426318 26.353722293778876 27.959503909353394 23.52411366644141 68-69 22.834491327344615 25.911524936373453 28.171494006206338 23.082489730075594 70-71 22.618486712463913 26.240469391420696 27.542645324697972 23.598398571417416 72-73 22.582720085798254 26.375336125594483 27.40801286973815 23.63393091886911 74-75 22.40677639610888 26.909102268083103 27.355117829429116 23.329003506378903 76-77 22.647433210900754 26.341027236260175 27.600954817952033 23.41058473488704 78-79 22.70259295027814 26.513326582621165 27.72103593062212 23.063044536478575 80-81 22.89632555906917 26.61858555525822 27.31041315464222 23.174675731030383 82-83 22.730790506374422 26.72485536342989 27.383369885657387 23.1609842445383 84-85 22.507086610893584 26.91102902874708 27.31603772479766 23.26584663556168 86-87 22.970525331534052 26.9172018692523 26.890832208783156 23.221440590430493 88-89 22.670887163321247 27.095536460353447 26.669747004479937 23.563829371845365 90-91 22.561283075549376 26.916940330270645 26.88364513412445 23.638131460055526 92-93 22.73102595887376 26.532836587845804 27.082408139656565 23.653729313623867 94-95 22.511464722598877 26.20008970297798 27.382706067602104 23.905739506821043 96-97 22.82568092797742 25.833782586705034 27.298422333224607 24.04211415209294 98-99 22.36437219140865 26.368317462614804 26.96159191536982 24.305718430606724 100-101 22.573100937540268 25.84294671132012 27.250858077775625 24.33309427336399 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1923.0 1 1287.5 2 950.5 3 1238.0 4 974.0 5 867.0 6 1800.0 7 3986.5 8 6418.5 9 7099.5 10 5366.5 11 3515.5 12 3138.5 13 3238.0 14 3313.5 15 3413.0 16 3486.0 17 3533.5 18 3688.0 19 3924.0 20 4206.5 21 4726.0 22 5464.5 23 6364.0 24 7343.0 25 8789.5 26 10703.5 27 12706.0 28 14538.0 29 16312.0 30 18173.0 31 21734.5 32 27859.0 33 29024.5 34 26624.5 35 27237.0 36 29460.0 37 32442.5 38 35737.5 39 38525.0 40 41312.5 41 45498.0 42 48062.5 43 50549.0 44 54497.5 45 59420.0 46 62819.0 47 67140.5 48 69854.0 49 68702.5 50 69636.5 51 70181.5 52 71402.0 53 75474.5 54 79763.5 55 76769.0 56 71601.0 57 67527.0 58 63367.5 59 57336.0 60 46321.0 61 38289.0 62 32633.5 63 24058.5 64 16903.0 65 13158.5 66 9832.5 67 7630.0 68 6002.5 69 4536.0 70 3303.0 71 2138.0 72 1491.5 73 1149.0 74 817.0 75 523.0 76 358.5 77 229.0 78 135.5 79 79.0 80 49.5 81 24.0 82 11.5 83 10.5 84 6.5 85 4.0 86 3.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01103715408023282 3 0.006507755943533503 4 0.0 5 2.6031023774134016E-4 6 1.5618614264480406E-4 7 0.0 8 1.0412409509653604E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002004388830608319 26-27 0.024417100300137703 28-29 3.644343328378762E-4 30-31 1.3015511887067008E-4 32-33 0.004008777661216638 34-35 0.018039499475474872 36-37 0.008876579106979698 38-39 0.024052665967299828 40-41 0.026265302988101216 42-43 0.02087688106685548 44-45 0.01121937124665176 46-47 0.0025770713536392674 48-49 2.6031023774134016E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.644343328378762E-4 68-69 3.384033090637422E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5618614264480406E-4 82-83 0.0011713960698360305 84-85 0.004451305065376917 86-87 8.850548083205565E-4 88-89 0.0 90-91 0.004555429160473453 92-93 8.329927607722883E-4 94-95 7.809307132240203E-5 96-97 2.8634126151547417E-4 98-99 2.3427921396720612E-4 100-101 0.003514188209508092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1920785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.679172087539165 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08415081116487 32.20660178876161 2 14.346249413203779 12.81957092686579 3 5.288444155068359 7.088499194389196 4 2.5150661547411524 4.494842941569014 5 1.4349377417074356 3.205591514832567 6 0.9151714343034649 2.453346120170673 7 0.6236683298916191 1.9505489245743055 8 0.43843879317699413 1.567126583216633 9 0.324364690157689 1.3043111229609037 >10 1.8030462603005368 14.904344444448297 >50 0.14032818748420167 4.323096304541959 >100 0.07503219770575646 6.297678390338747 >500 0.005843068996948885 1.8099009304305809 >1k 0.004908177957437064 3.8349181726940142 >5k 1.1686137993897772E-4 0.39556339282662367 >10k+ 2.3372275987795544E-4 1.3440592473789525 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14250 0.7418841775628193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11492 0.5982970504246962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7576 0.39442207222567854 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3379 0.17591765866559764 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3336 0.17367899062102213 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3101 0.16144440944717917 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2989 0.15561346012177313 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2938 0.15295829569681146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2668 0.1389015428587791 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2498 0.13005099477557353 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.12541747254377766 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2333 0.1214607569301093 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2149 0.11188134018122799 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2062 0.10735194204452866 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.206204754826803E-5 2 0.0 0.0 0.0 0.0 5.206204754826803E-5 3 0.0 0.0 0.0 0.0 5.206204754826803E-5 4 0.0 0.0 0.0 0.0 5.206204754826803E-5 5 0.0 0.0 0.0 0.0 5.206204754826803E-5 6 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 7 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 8 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 9 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 10-11 5.206204754826803E-5 0.0 0.0 0.0 5.206204754826803E-5 12-13 1.3015511887067008E-4 0.0 0.0 0.0 5.206204754826803E-5 14-15 2.082481901930721E-4 0.0 0.0 0.0 1.0412409509653605E-4 16-17 2.342792139672061E-4 0.0 0.0 2.6031023774134014E-5 1.0412409509653605E-4 18-19 2.603102377413401E-4 0.0 0.0 1.0412409509653605E-4 1.0412409509653605E-4 20-21 2.603102377413401E-4 0.0 0.0 1.0412409509653605E-4 1.822171664189381E-4 22-23 2.603102377413401E-4 0.0 0.0 2.3427921396720615E-4 2.342792139672061E-4 24-25 3.644343328378762E-4 0.0 0.0 6.507755943533503E-4 2.603102377413401E-4 26-27 3.644343328378762E-4 0.0 0.0 0.0018742337117376492 2.603102377413401E-4 28-29 4.164963803861442E-4 0.0 0.0 0.005856980349180153 2.603102377413401E-4 30-31 4.164963803861442E-4 0.0 0.0 0.01939311271172984 2.8634126151547417E-4 32-33 4.164963803861442E-4 0.0 0.0 0.04368005789299688 4.6855842793441224E-4 34-35 4.164963803861442E-4 0.0 0.0 0.07705183037143667 5.206204754826802E-4 36-37 4.6855842793441224E-4 0.0 0.0 0.13096208060766823 5.206204754826802E-4 38-39 5.726825230309483E-4 0.0 0.0 0.21420929463734878 5.206204754826802E-4 40-41 5.726825230309483E-4 0.0 0.0 0.3033915820875319 5.206204754826802E-4 42-43 7.809307132240204E-4 0.0 0.0 0.3921573731573289 5.466514992568143E-4 44-45 8.329927607722884E-4 0.0 0.0 0.4976611125138941 5.987135468050824E-4 46-47 0.0010152099271912266 0.0 0.0 0.6187314040873914 7.288686656757524E-4 48-49 0.0010412409509653604 0.0 0.0 0.7444872799402328 7.288686656757524E-4 50-51 0.0010412409509653604 0.0 0.0 0.8857316149386839 7.288686656757524E-4 52-53 0.0010412409509653604 0.0 0.0 1.0142207482878094 7.288686656757524E-4 54-55 0.0010412409509653604 0.0 0.0 1.1461459767751205 7.809307132240204E-4 56-57 0.0011453650460618967 0.0 0.0 1.301967685087087 8.329927607722884E-4 58-59 0.0011453650460618967 0.0 0.0 1.4655987005312932 8.329927607722884E-4 60-61 0.0011453650460618967 0.0 0.0 1.6410998628165046 8.329927607722884E-4 62-63 0.0011453650460618967 0.0 0.0 1.8132950850824012 8.850548083205565E-4 64-65 0.0011453650460618967 0.0 0.0 1.986713765465682 8.850548083205565E-4 66-67 0.0012494891411584327 0.0 0.0 2.1878554861684156 8.850548083205565E-4 68-69 0.0012494891411584327 0.0 0.0 2.4107851737700994 8.850548083205565E-4 70-71 0.0012494891411584327 0.0 0.0 2.636864615248453 0.0010412409509653604 72-73 0.0013015511887067006 0.0 0.0 2.8663541208412187 0.0011453650460618967 74-75 0.0013536132362549688 0.0 0.0 3.098576883930268 0.0011453650460618967 76-77 0.0013536132362549688 0.0 0.0 3.3425917007890003 0.0011713960698360305 78-79 0.0013536132362549688 0.0 0.0 3.622425206360941 0.0011974270936101646 80-81 0.0014056752838032367 0.0 0.0 3.9072566684975154 0.0012494891411584325 82-83 0.0014056752838032367 0.0 0.0 4.19927269319575 0.0013536132362549688 84-85 0.0014317063075773707 0.0 0.0 4.502508089140639 0.0013536132362549688 86-87 0.0014837683551256389 0.0 0.0 4.812615675361896 0.0014056752838032369 88-89 0.0015097993788997727 0.0 0.0 5.139305023727278 0.0014577373313515048 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5360 0.0 57.874184 1 GTATCAA 9790 0.0 43.574272 1 TATCAAC 12460 0.0 34.122547 2 ATCAACG 12770 0.0 33.32619 3 TCAACGC 12900 0.0 33.17272 4 CAACGCA 13190 0.0 32.47938 5 AACGCAG 13525 0.0 31.710018 6 GTGGTAT 2270 0.0 30.553677 1 TGGTATC 2280 0.0 29.794605 2 ACGCAGA 15360 0.0 27.828981 7 CGCAGAG 15380 0.0 27.576626 8 GCAGAGT 16785 0.0 25.15512 9 TAAGGTA 560 0.0 24.59552 4 GAGTACT 10790 0.0 23.329231 12-13 CAGAGTA 15880 0.0 22.595917 10-11 GTACTTT 11110 0.0 22.486282 14-15 TACATGG 8820 0.0 22.459698 2 TAAGGTG 1155 0.0 22.205359 5 AGGTAAG 1030 0.0 22.138105 2 GTACATG 9080 0.0 22.130487 1 >>END_MODULE