FastQCFastQC Report
Thu 26 May 2016
SRR938025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966771
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149010.7576377727757833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115170.5855791040238035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77900.39608068249938605No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34420.17500766484761063No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34000.17287218491629172No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30950.15736453303409498No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30890.15705946447247798No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27120.13789098985087742No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.13621311276198397No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24800.12609500546835398No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23610.12004447899628375No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23400.1189767390306243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.11536676105148999No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21180.10768920225079584No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT19800.10067262533360519No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54850.053.1138881
GTATCAA100400.043.5253451
TATCAAC129100.033.789122
TCAACGC132750.032.9325074
ATCAACG132700.032.8374863
CAACGCA134000.032.5898365
GTGGTAT25100.032.536981
TGGTATC25100.032.1836742
AACGCAG138900.031.4401636
TAAGGTG12650.030.803135
CGCAGAG153650.028.1586998
ACGCAGA154200.028.1507637
AGGTAAG11900.027.55262
GTAAGGT13300.026.7966884
GCAGAGT165550.025.7011959
TACATGG91850.024.9878232
GTACATG95100.024.9089851
GGTAAGG14050.024.6898273
AAGGTAA13100.024.0617661
TAAGGTA6350.023.9468174
Produced by FastQC (version 0.11.5)