##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938025_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1966771 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.956079279184003 33.0 31.0 34.0 30.0 34.0 2 32.124074943142844 34.0 31.0 34.0 30.0 34.0 3 32.1838160111167 34.0 31.0 34.0 30.0 34.0 4 35.71289946821465 37.0 35.0 37.0 35.0 37.0 5 35.73621585837904 37.0 35.0 37.0 35.0 37.0 6 35.773093563002504 37.0 36.0 37.0 35.0 37.0 7 35.71777751451491 37.0 36.0 37.0 35.0 37.0 8 35.701244323818074 37.0 35.0 37.0 35.0 37.0 9 37.36726238082624 39.0 37.0 39.0 35.0 39.0 10-11 37.29869949272182 39.0 37.0 39.0 34.0 39.0 12-13 37.19642881657295 39.0 37.0 39.0 34.0 39.0 14-15 38.44333656536526 40.0 38.0 41.0 34.0 41.0 16-17 38.3715941001774 40.0 38.0 41.0 34.0 41.0 18-19 38.33860500281934 40.0 38.0 41.0 34.0 41.0 20-21 38.24003455409908 40.0 38.0 41.0 34.0 41.0 22-23 38.101873070123574 40.0 38.0 41.0 33.0 41.0 24-25 38.034617655029486 40.0 38.0 41.0 33.0 41.0 26-27 38.02207603223761 40.0 38.0 41.0 33.0 41.0 28-29 37.92700294035249 40.0 38.0 41.0 33.0 41.0 30-31 37.708304881452904 40.0 37.5 41.0 33.0 41.0 32-33 37.44068145198399 40.0 37.0 41.0 32.5 41.0 34-35 37.219976804620366 40.0 36.0 41.0 31.5 41.0 36-37 36.9839630541634 40.0 36.0 41.0 31.0 41.0 38-39 36.72663467175386 39.5 35.5 41.0 30.5 41.0 40-41 36.479414481909686 39.0 35.0 41.0 30.0 41.0 42-43 36.15786560814655 39.0 35.0 41.0 29.5 41.0 44-45 35.889511285248766 39.0 35.0 40.5 28.5 41.0 46-47 35.67860671120329 39.0 35.0 41.0 27.5 41.0 48-49 35.353483450793206 39.0 35.0 41.0 26.0 41.0 50-51 34.65504245283259 38.5 34.0 40.0 25.0 40.5 52-53 34.689623753858484 38.0 34.5 40.0 25.5 41.0 54-55 34.7903177339914 38.0 35.0 40.0 25.0 41.0 56-57 34.53964493070113 38.0 34.0 40.0 24.0 41.0 58-59 34.24338191889142 38.0 34.0 40.0 23.0 41.0 60-61 33.84236929464589 37.0 34.0 40.0 22.0 41.0 62-63 33.407359321446165 36.5 33.5 40.0 21.5 41.0 64-65 32.9571915083149 36.0 33.0 39.0 19.5 41.0 66-67 32.5128288956874 35.0 32.5 39.0 18.0 40.5 68-69 32.020083680306456 35.0 32.0 38.5 14.5 40.0 70-71 31.531753569683506 35.0 31.5 37.5 9.5 40.0 72-73 31.07147578442025 35.0 31.0 37.0 7.0 39.0 74-75 30.567715051726918 35.0 31.0 36.0 3.5 39.0 76-77 30.059168555973216 34.5 30.0 36.0 2.0 38.5 78-79 29.529439624643643 34.0 29.5 35.0 2.0 37.0 80-81 29.044622124283915 34.0 29.0 35.0 2.0 37.0 82-83 28.542376819670416 34.0 29.0 35.0 2.0 36.0 84-85 28.083903769172924 34.0 27.5 35.0 2.0 36.0 86-87 27.618080600130874 33.5 26.5 35.0 2.0 35.5 88-89 27.077020151303838 33.0 25.5 35.0 2.0 35.0 90-91 26.48232966623974 33.0 24.5 35.0 2.0 35.0 92-93 25.887493765161274 32.0 23.5 35.0 2.0 35.0 94-95 25.30621460251346 32.0 19.5 34.5 2.0 35.0 96-97 24.483120810709533 32.0 12.5 34.0 2.0 35.0 98-99 23.41515407741928 31.0 2.0 34.0 2.0 35.0 100-101 20.558430544277904 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35678298557264654 1101 2 0.18677212832843537 1101 3 0.27721344794484537 1101 4 0.14959249779089845 1101 5 0.1710403150432569 1101 6 0.16223866324843073 1101 7 0.1723141999218143 1101 8 0.18798834325956904 1101 9 0.20409255869977017 1101 10-11 0.2592484073585055 1101 12-13 0.12384318506965286 1101 14-15 0.1706589727400143 1101 16-17 0.3184588485467401 1101 18-19 0.20796255575404388 1101 20-21 0.3051056254833995 1101 22-23 0.34445078134616836 1101 24-25 0.3790549854198346 1101 26-27 0.40926888951479157 1101 28-29 0.40693711504253116 1101 30-31 0.4690849003785047 1101 32-33 0.5274615746340743 1101 34-35 0.43573799235444 1101 36-37 0.46692747771053433 1101 38-39 0.5519472202467455 1101 40-41 0.6619820142896415 1101 42-43 0.625888434161304 1101 44-45 0.6481075990393066 1101 46-47 0.5256481586033672 1101 48-49 0.7577904591464915 1101 50-51 0.7670649273110755 1101 52-53 0.8618279142030048 1101 54-55 0.9043152780411887 1101 56-57 0.7570213218101145 1101 58-59 0.7205341064906747 1101 60-61 0.5675581427336027 1101 62-63 0.6645907165269733 1101 64-65 0.5868175307644563 1101 66-67 0.5180549955776712 1101 68-69 0.6374688358634799 1101 70-71 0.7286918187131626 1101 72-73 0.8779345349083343 1101 74-75 0.7785539132684214 1101 76-77 0.8892054897678889 1101 78-79 0.7614958456039496 1101 80-81 0.8603566905661744 1101 82-83 0.7745077758210925 1101 84-85 0.6645260011670686 1101 86-87 0.823749088697749 1101 88-89 0.6997917610698963 1101 90-91 0.8683700347242862 1101 92-93 0.9611745323971306 1101 94-95 1.0820697240241763 1101 96-97 1.0584167049525561 1101 98-99 1.220184511832791 1101 100-101 1.2839450118409488 1102 1 0.4466324695057615 1102 2 0.30638768149398743 1102 3 0.1496468171282217 1102 4 0.16568685942525718 1102 5 0.16510953950636775 1102 6 0.23171769296808975 1102 7 0.08827276422309183 1102 8 0.16725853439239557 1102 9 0.31331672567040414 1102 10-11 0.3155857918318219 1102 12-13 0.1745675751420137 1102 14-15 0.29247934609867343 1102 16-17 0.21921047496824997 1102 18-19 0.1989235902626305 1102 20-21 0.3194691335023876 1102 22-23 0.3108925523666386 1102 24-25 0.156733053196632 1102 26-27 0.28508907664688365 1102 28-29 0.2566343260005084 1102 30-31 0.28072184055620397 1102 32-33 0.3072341219317849 1102 34-35 0.29810316673114556 1102 36-37 0.36202197346221254 1102 38-39 0.2960559537892564 1102 40-41 0.4347672207235078 1102 42-43 0.5235775730228269 1102 44-45 0.39148281626805925 1102 46-47 0.3056976253945365 1102 48-49 0.25227043566030005 1102 50-51 0.3126692254433152 1102 52-53 0.2943881224806404 1102 54-55 0.37357109214036655 1102 56-57 0.39047843602139665 1102 58-59 0.4483847470787836 1102 60-61 0.3867791176553652 1102 62-63 0.37781274673270104 1102 64-65 0.33872357689227073 1102 66-67 0.2269028367873318 1102 68-69 0.1713199303432873 1102 70-71 0.26490477307825344 1102 72-73 0.3266031999513572 1102 74-75 0.2950966139695588 1102 76-77 0.18647522017501927 1102 78-79 0.28394839270802663 1102 80-81 0.2619416382191382 1102 82-83 0.4008156687242952 1102 84-85 0.45014062433872226 1102 86-87 0.4449324760339408 1102 88-89 0.4265656190724165 1102 90-91 0.5298925480655434 1102 92-93 0.40152037850506517 1102 94-95 0.3633594199708412 1102 96-97 0.438610796330579 1102 98-99 0.5497525943055379 1102 100-101 0.6371924873175558 1103 1 0.27196389422944733 1103 2 0.2521915061798552 1103 3 0.24471950316384294 1103 4 0.17131649486827882 1103 5 0.1690899879751342 1103 6 0.09599658105854303 1103 7 0.23945377775367405 1103 8 0.19864322741934615 1103 9 0.2566018069321032 1103 10-11 0.1794259504066531 1103 12-13 0.15953877295806507 1103 14-15 0.32184383958272633 1103 16-17 0.3362793148992864 1103 18-19 0.2606674120109176 1103 20-21 0.24197601215443143 1103 22-23 0.3158948863531563 1103 24-25 0.4537398551391405 1103 26-27 0.37577964507956096 1103 28-29 0.41988176372476715 1103 30-31 0.4500131179866784 1103 32-33 0.46172577203709153 1103 34-35 0.5578204123709014 1103 36-37 0.6523926508524056 1103 38-39 0.610480044999214 1103 40-41 0.5600529739771289 1103 42-43 0.8434851019210683 1103 44-45 0.6728577673197407 1103 46-47 0.741976250792284 1103 48-49 0.8789753835209737 1103 50-51 0.7936906313561352 1103 52-53 0.9149038503887681 1103 54-55 0.9960439351921053 1103 56-57 0.7485900406350865 1103 58-59 0.9247345011586319 1103 60-61 0.9477712404167988 1103 62-63 0.8783701512197837 1103 64-65 0.7804090666901757 1103 66-67 0.881788117194084 1103 68-69 0.927172949960493 1103 70-71 1.0873046126286035 1103 72-73 1.0451678895284289 1103 74-75 0.8483457057858388 1103 76-77 0.939275390870776 1103 78-79 0.8203192058046191 1103 80-81 0.8109742941377469 1103 82-83 0.7562759572993158 1103 84-85 0.8631573203130642 1103 86-87 0.8923154544732448 1103 88-89 0.7723508318167163 1103 90-91 0.8161973473576865 1103 92-93 0.8589434502820303 1103 94-95 1.0967978576359592 1103 96-97 0.9211056249085985 1103 98-99 1.0695807158864774 1103 100-101 1.2184134470342833 1104 1 0.3504889198310899 1104 2 0.2652563423293728 1104 3 0.22881448524269388 1104 4 0.23088030695225825 1104 5 0.17852259166176765 1104 6 0.15333625079740898 1104 7 0.2516945611638093 1104 8 0.20767580550434417 1104 9 0.09455143570834679 1104 10-11 0.23586956279784488 1104 12-13 0.14870607966569338 1104 14-15 0.27542486876859584 1104 16-17 0.43622171121264586 1104 18-19 0.4528877141102541 1104 20-21 0.33843418547753146 1104 22-23 0.3593736689952465 1104 24-25 0.5119715819696538 1104 26-27 0.42169858389164006 1104 28-29 0.42769426372476715 1104 30-31 0.3593353146072644 1104 32-33 0.283870389292602 1104 34-35 0.45056554805957916 1104 36-37 0.49031427783653925 1104 38-39 0.48419196256194397 1104 40-41 0.5258827870851661 1104 42-43 0.46407681979101056 1104 44-45 0.4793693840632116 1104 46-47 0.5432635331783544 1104 48-49 0.4432594977042825 1104 50-51 0.5501750463586959 1104 52-53 0.470939436666292 1104 54-55 0.5924021021147823 1104 56-57 0.5151150982387733 1104 58-59 0.4006049568677952 1104 60-61 0.39751602408294673 1104 62-63 0.5254315631501498 1104 64-65 0.5835724691479314 1104 66-67 0.7026006491961283 1104 68-69 0.70953102087703 1104 70-71 0.8175313471396102 1104 72-73 0.699409761008198 1104 74-75 0.6050221330771848 1104 76-77 0.7206333977832173 1104 78-79 0.8296238012987338 1104 80-81 0.8519889190737437 1104 82-83 0.7962185136229216 1104 84-85 0.7631669209275067 1104 86-87 0.8565571659427782 1104 88-89 0.8765775023236273 1104 90-91 0.7800430334816006 1104 92-93 0.870808209626631 1104 94-95 0.9096898827255657 1104 96-97 1.0336808297217104 1104 98-99 1.1241522084620037 1104 100-101 0.8925125893793933 1105 1 0.2092844811722081 1105 2 0.19718023742716184 1105 3 0.1794907615952468 1105 4 0.2156521827460054 1105 5 0.14149011134680478 1105 6 0.12630504048498636 1105 7 0.12541662110793084 1105 8 0.08518009176291486 1105 9 0.16815998917545016 1105 10-11 0.18618468217808726 1105 12-13 0.11007418990191553 1105 14-15 0.29126815045515286 1105 16-17 0.2402603192898667 1105 18-19 0.25402912889308027 1105 20-21 0.287473349180857 1105 22-23 0.20406624900540749 1105 24-25 0.2891635269785482 1105 26-27 0.2824765398225537 1105 28-29 0.24393084932293618 1105 30-31 0.316512416461471 1105 32-33 0.3552862039992277 1105 34-35 0.39054205301258804 1105 36-37 0.4421911034651842 1105 38-39 0.4491121111522389 1105 40-41 0.4796904134504203 1105 42-43 0.4053193585260928 1105 44-45 0.46689359348662407 1105 46-47 0.4170709214181372 1105 48-49 0.415902849240986 1105 50-51 0.2903940424216884 1105 52-53 0.33423160075061276 1105 54-55 0.27423471578001823 1105 56-57 0.3349910348656522 1105 58-59 0.3720060871700568 1105 60-61 0.3591202754164584 1105 62-63 0.4894924756469692 1105 64-65 0.4735069052668024 1105 66-67 0.6190781384011075 1105 68-69 0.7456288586977067 1105 70-71 0.7326138179295505 1105 72-73 0.6660444130274996 1105 74-75 0.7110415482410168 1105 76-77 0.6836386206686633 1105 78-79 0.7709298401608073 1105 80-81 0.8446447325103712 1105 82-83 0.8950147893404719 1105 84-85 0.8591711754360141 1105 86-87 0.8500622935780378 1105 88-89 0.9251173962784165 1105 90-91 0.9542278506012352 1105 92-93 1.0384287011176134 1105 94-95 1.081088631773067 1105 96-97 1.1322211205522876 1105 98-99 1.1272279958635814 1105 100-101 1.0311928979900102 1106 1 0.0468865409775141 1106 2 0.027970314073783697 1106 3 0.11172712163665466 1106 4 0.17327991742540405 1106 5 0.09416605547269796 1106 6 0.0553591796740136 1106 7 0.1883631933329326 1106 8 0.07867705032256822 1106 9 0.06847552165043425 1106 10-11 -0.4716237167720507 1106 12-13 -0.18913550083652098 1106 14-15 -0.09758927887836677 1106 16-17 0.16326404694429186 1106 18-19 0.1784971191387612 1106 20-21 0.004870426761819147 1106 22-23 -0.08775295006257977 1106 24-25 0.008185880305127569 1106 26-27 0.12359253907818868 1106 28-29 0.03943990289027255 1106 30-31 0.12500126257504718 1106 32-33 0.09423675200125814 1106 34-35 -0.050808814641136735 1106 36-37 -0.12701765681348576 1106 38-39 0.03511857784759087 1106 40-41 0.3499323475273499 1106 42-43 0.358078414031894 1106 44-45 0.5244212068185661 1106 46-47 0.8137081089871927 1106 48-49 0.6617154168447001 1106 50-51 0.7787762588156184 1106 52-53 0.7121214779299265 1106 54-55 0.3828249432386528 1106 56-57 0.551559906334262 1106 58-59 0.6091249136664416 1106 60-61 0.43871650434057585 1106 62-63 0.6021761202966331 1106 64-65 0.48921519837713134 1106 66-67 0.31952546177852525 1106 68-69 -0.11244719281874893 1106 70-71 0.40063425509056216 1106 72-73 0.6639436093428799 1106 74-75 0.5838993100200547 1106 76-77 0.6384576510908246 1106 78-79 0.649457954584097 1106 80-81 0.7845149818348176 1106 82-83 0.6992674993045327 1106 84-85 0.7412133504632514 1106 86-87 0.8401932412513027 1106 88-89 0.6873106595558802 1106 90-91 0.6292331571295726 1106 92-93 0.6243155891949357 1106 94-95 0.45055918078309176 1106 96-97 0.5384915955132286 1106 98-99 0.37985291008682154 1106 100-101 0.010081153649338148 1107 1 0.2281892295330401 1107 2 0.026983237337340427 1107 3 0.07170207263384043 1107 4 -0.015250335142681593 1107 5 0.11263618481766002 1107 6 0.04624568597463963 1107 7 3.4896009879048506E-5 1107 8 0.026222276128656574 1107 9 0.09356828533424988 1107 10-11 0.171753976510594 1107 12-13 0.06208535723223463 1107 14-15 0.11774134736542408 1107 16-17 0.2040525710188561 1107 18-19 0.23439835436537493 1107 20-21 0.1565753221756765 1107 22-23 0.193992635939928 1107 24-25 0.2786707647857938 1107 26-27 0.2349124698845273 1107 28-29 0.2725050312336563 1107 30-31 0.29135037041493916 1107 32-33 0.30551598753864084 1107 34-35 0.3528139535544952 1107 36-37 0.4212953967498194 1107 38-39 0.47149916543204995 1107 40-41 0.43490975568568047 1107 42-43 0.48451983156581946 1107 44-45 0.326504832671084 1107 46-47 0.30535551619074397 1107 48-49 0.27989363544499213 1107 50-51 0.17579885814576812 1107 52-53 0.11888344500678016 1107 54-55 0.26051506666771473 1107 56-57 0.254525629638259 1107 58-59 0.13252878858617834 1107 60-61 0.1511755282531908 1107 62-63 0.281339089520813 1107 64-65 0.26601873126003284 1107 66-67 0.25723736267583774 1107 68-69 0.31201534413227705 1107 70-71 0.4678968184385148 1107 72-73 0.3835846812829473 1107 74-75 0.3642087438339594 1107 76-77 0.5108266165370523 1107 78-79 0.353428305775612 1107 80-81 0.3980637918199754 1107 82-83 0.24245666982309189 1107 84-85 0.2924927825735253 1107 86-87 0.48536232202029694 1107 88-89 0.5710084249901293 1107 90-91 0.5248666413560024 1107 92-93 0.5301533126561893 1107 94-95 0.37675530342960073 1107 96-97 0.5337210756434558 1107 98-99 0.6332420373593415 1107 100-101 0.6387083915381062 1108 1 0.21428193189252553 1108 2 0.1668188914074875 1108 3 0.13412316696521742 1108 4 0.15541730399971243 1108 5 0.19092217783509113 1108 6 0.12095107395914084 1108 7 0.15337244381606752 1108 8 0.09275988613379837 1108 9 0.06889094241437732 1108 10-11 0.09913253119939469 1108 12-13 -0.05921139842583756 1108 14-15 -0.008426271414215591 1108 16-17 0.16058526619584512 1108 18-19 0.15077755140648463 1108 20-21 0.21262611428677758 1108 22-23 0.08481866027897667 1108 24-25 0.11761689049432533 1108 26-27 0.15455780625869409 1108 28-29 0.0789184191885326 1108 30-31 0.16953158781139877 1108 32-33 0.18669400057821406 1108 34-35 0.13783488091129925 1108 36-37 0.14880543003366853 1108 38-39 0.2763073668085738 1108 40-41 0.08453457195810898 1108 42-43 0.29082076158829295 1108 44-45 0.2137539542959601 1108 46-47 0.19455737956777597 1108 48-49 0.2224280947768733 1108 50-51 0.2928798297082551 1108 52-53 0.2398423640777878 1108 54-55 0.2919910238096648 1108 56-57 0.28619341317108393 1108 58-59 0.3257824233376283 1108 60-61 0.24275284562706645 1108 62-63 0.30354768001103594 1108 64-65 0.29196944043919615 1108 66-67 0.2853939596525237 1108 68-69 0.34229591510386825 1108 70-71 0.31221986796443346 1108 72-73 0.40843395063033583 1108 74-75 0.5209098482356502 1108 76-77 0.5416113970450809 1108 78-79 0.5867072458757967 1108 80-81 0.7824332036325892 1108 82-83 0.6320549127026069 1108 84-85 0.7404231401064507 1108 86-87 0.7543802079222388 1108 88-89 0.7642997771139655 1108 90-91 0.6321309147204133 1108 92-93 0.6189555107228379 1108 94-95 0.8414668835422283 1108 96-97 0.9496615479112371 1108 98-99 0.9150632357258779 1108 100-101 0.6569548067679634 1109 1 0.3071916364626581 1109 2 0.2662646833305544 1109 3 0.2583486189818345 1109 4 0.2018634767538927 1109 5 0.26268160153560416 1109 6 0.2590378703362717 1109 7 0.3110984815007214 1109 8 0.37623630264659624 1109 9 0.3129964726909833 1109 10-11 0.3853885340964851 1109 12-13 0.33649749491528524 1109 14-15 0.35911634241182355 1109 16-17 0.3484332820016718 1109 18-19 0.495525580618839 1109 20-21 0.27595063633840056 1109 22-23 0.6123921945999768 1109 24-25 0.475060135709505 1109 26-27 0.4004569282106445 1109 28-29 0.4506711396205745 1109 30-31 0.49069612981791977 1109 32-33 0.4169919995858038 1109 34-35 0.352322112920767 1109 36-37 0.3677723682316625 1109 38-39 0.40053228470021196 1109 40-41 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66-67 -0.4000539206963438 2316 68-69 -0.49899832500110364 2316 70-71 -0.4624585722152297 2316 72-73 -0.4670888949057783 2316 74-75 -0.6013790766002352 2316 76-77 -0.5486598369838092 2316 78-79 -0.6157727040776102 2316 80-81 -0.6552635450839617 2316 82-83 -0.8287534845849649 2316 84-85 -0.8496242977104878 2316 86-87 -0.9452741512615219 2316 88-89 -0.8891315030527167 2316 90-91 -0.7467826833642803 2316 92-93 -0.8893284391188878 2316 94-95 -1.1572344004285569 2316 96-97 -1.4699594032532026 2316 98-99 -1.4396358739752735 2316 100-101 -1.5180496866062683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5205.0 3 1341.0 4 1324.0 5 1602.0 6 1859.0 7 2293.0 8 2784.0 9 3491.0 10 4411.0 11 5678.0 12 7468.0 13 9187.0 14 10681.0 15 13485.0 16 17339.0 17 24151.0 18 21337.0 19 16223.0 20 15163.0 21 15424.0 22 16281.0 23 17759.0 24 19731.0 25 22026.0 26 24666.0 27 28630.0 28 33808.0 29 40263.0 30 48427.0 31 58690.0 32 74039.0 33 96749.0 34 135683.0 35 205693.0 36 304935.0 37 386671.0 38 247367.0 39 24907.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08734081876461 17.58032258822736 14.33354147015451 22.998795122853526 2 16.13372226116701 19.372899652345726 39.29342855078937 25.199949535697897 3 19.552688933522994 23.557548488714968 29.251805544532694 27.637957033229345 4 13.152447996483238 15.875038922503007 35.207922484690485 35.76459059632327 5 14.011159425083516 36.9329645753134 33.095862667321406 15.960013332281678 6 33.398860919479965 34.898835214070075 16.26210762982377 15.44019623662619 7 29.722137563047056 32.953952814622184 18.97921895678074 18.344690665550015 8 27.973735912329595 33.276763903005815 18.693347903013947 20.056152281650647 9 27.41641909490477 16.067983884095717 19.262422676216833 37.25317434478268 10-11 25.896707279623886 25.895715044247336 25.60974772033922 22.597829955789553 12-13 27.111793875683233 23.35184636575481 26.48717627644228 23.049183482119677 14-15 23.627403822306718 24.368000231984862 25.2644741892676 26.74012175644082 16-17 22.669657198007464 27.773911149980897 26.1968612140092 23.35957043800244 18-19 22.7938009823616 25.545776936404437 29.24696888102914 22.413453200204824 20-21 23.131005306728284 24.541137702704148 30.01318922208523 22.314667768482337 22-23 23.145472306737354 24.45152788660161 29.890025744609407 22.512974062051633 24-25 22.874385704917998 24.76010270970513 29.77141212612703 22.594099459249843 26-27 21.943681807913286 25.270605941577408 29.75795307224367 23.02775917826564 28-29 22.440563080447117 25.74245587291767 29.45401019536451 22.362970851270706 30-31 22.319565243385398 25.165500238000803 29.298318678878022 23.21661583973578 32-33 22.04275565865385 25.164825966930245 29.42245591771969 23.369962456696214 34-35 23.08445967777636 25.34207576422914 29.183689253597834 22.389775304396675 36-37 23.13313265946805 24.742517724325804 29.09875352222321 23.02559609398294 38-39 22.523787772276272 25.215598145606698 28.986899812098727 23.273714270018303 40-41 22.980232350821915 25.038132098184846 29.221738945760983 22.759896605232257 42-43 22.604879475554934 25.02612687653795 28.92266215640519 23.44633149150193 44-45 22.564106345691645 25.381444326571877 29.219357568520632 22.835091759215846 46-47 23.183791628448212 25.83553150374691 28.2461486350576 22.734528232747277 48-49 22.83653564964071 25.883733006210353 27.98442504016564 23.295306303983303 50-51 23.074775281527465 26.25228990590152 27.921950445469978 22.750984367101033 52-53 23.50002593790973 25.77409402950005 27.902858967956 22.82302106463422 54-55 22.813631842997363 26.21948195904405 27.96805264072012 22.99883355723847 56-57 22.841637524691848 26.30800677029614 27.845640096102404 23.004715608909613 58-59 22.91044943111659 25.66881688931744 27.77026793360959 23.65046574595638 60-61 22.441508849549646 26.15443143751047 28.187263229821784 23.216796483118095 62-63 22.63648959658494 26.133324721775047 28.396503266586127 22.833682415053886 64-65 22.308217694187817 26.247865299509034 28.126427159798162 23.31748984650499 66-67 22.22912987704798 26.223246080753455 28.11557057440752 23.432053467791047 68-69 22.933709930504296 25.80210188211285 28.24666945181769 23.01751873556517 70-71 22.706323609340302 26.16774771791493 27.66921484981132 23.456713822933445 72-73 22.705747711088506 26.37525432349949 27.43179043743642 23.487207527975585 74-75 22.5202868914473 26.95414472083066 27.483616756848996 23.04195163087305 76-77 22.92645875650588 26.34170914073045 27.4983751191325 23.233456983631168 78-79 22.957724777024428 26.579212202976805 27.56694606734602 22.896116952652747 80-81 23.17232583442011 26.69061179323758 27.182693806397907 22.954368565944407 82-83 22.990461050093092 26.784020618818843 27.206781671098717 23.01873665998935 84-85 22.82951298869712 27.00274409810405 27.145161569201008 23.02258134399782 86-87 23.318166401313157 27.030106902462624 26.710182608302112 22.941544087922104 88-89 23.11811767003882 27.105036665066045 26.538528839658497 23.238316825236634 90-91 23.008761543323793 27.054155215019644 26.58142105135976 23.3556621902968 92-93 23.258781148711456 26.55303911732655 26.88687726419041 23.30130246977159 94-95 23.151806299715723 26.32038545518183 27.10332221586874 23.42448602923371 96-97 23.516220569530187 25.902068967972923 26.98280442355816 23.59890603893873 98-99 23.209283896664974 26.355787761479622 26.558272819242028 23.876655522613373 100-101 23.631248978144907 25.707480259414943 26.92176244473069 23.739508317709458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2000.0 1 1353.5 2 1047.0 3 1354.0 4 1036.5 5 944.0 6 1788.0 7 3989.5 8 6650.5 9 7382.0 10 5498.5 11 3606.0 12 3253.5 13 3300.5 14 3383.5 15 3468.0 16 3577.5 17 3700.5 18 3851.0 19 4087.0 20 4399.0 21 4951.0 22 5785.0 23 6721.5 24 7762.5 25 9395.0 26 11358.0 27 13200.0 28 14823.5 29 16601.0 30 18359.0 31 21660.0 32 28126.0 33 29689.5 34 27108.5 35 27721.5 36 30153.5 37 33302.5 38 36740.5 39 39411.0 40 41906.5 41 46067.0 42 48939.5 43 51530.0 44 55052.0 45 60338.5 46 64292.0 47 68111.0 48 70783.5 49 70046.5 50 70830.5 51 71333.5 52 72630.5 53 76637.0 54 80870.0 55 78147.0 56 73139.0 57 69162.5 58 65520.5 59 59120.5 60 47541.0 61 39578.5 62 33843.0 63 25125.5 64 18127.0 65 14387.5 66 10740.0 67 8119.5 68 6412.0 69 4908.5 70 3640.5 71 2461.5 72 1644.5 73 1283.5 74 962.0 75 646.5 76 466.5 77 315.0 78 187.5 79 118.5 80 73.5 81 43.0 82 29.5 83 25.0 84 19.0 85 14.0 86 10.0 87 9.0 88 8.5 89 5.5 90 4.5 91 4.0 92 4.5 93 5.0 94 3.0 95 1.5 96 2.5 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49446529362086383 2 0.05211587927623501 3 0.058929077152347686 4 0.06747099687762327 5 0.08216513259550808 6 0.11206185163397264 7 0.06986070061028966 8 0.053132774481624956 9 0.05140405263246204 10-11 0.07685185514734558 12-13 0.11231607543532013 14-15 0.05722577768331952 16-17 0.06274243417256 18-19 0.06108997946380133 20-21 0.07779248321233127 22-23 0.06665748071331132 24-25 0.09230866226927284 26-27 0.06950478728840317 28-29 0.06955563204867267 30-31 0.07329272192848074 32-33 0.07324187716821125 34-35 0.07575869280155138 36-37 0.07082675105541011 38-39 0.042963822427725444 40-41 0.04614161994456904 42-43 0.0449213456981011 44-45 0.021812402155614453 46-47 0.02809173004889741 48-49 0.027354481024989694 50-51 0.027100257223642205 52-53 0.027303636264720193 54-55 0.027278213884585446 56-57 0.0272019467441812 58-59 0.023541124004777373 60-61 0.030379744261024796 62-63 0.021431066453593224 64-65 0.023032676402082397 66-67 0.01797362275526739 68-69 0.019854878885238802 70-71 0.04375191621190266 72-73 0.039201310167782624 74-75 0.03363380891827264 76-77 0.02404957160747235 78-79 0.015253428080849271 80-81 0.016804193269068946 82-83 0.020948041231033 84-85 0.0187617165394446 86-87 0.010779089177133485 88-89 0.04408240715365439 90-91 0.054836073950653125 92-93 0.03551506504824405 94-95 0.05567501249509984 96-97 0.04512472473917909 98-99 0.035591332188648295 100-101 0.17439752772437667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1966771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.243700128068795 #Duplication Level Percentage of deduplicated Percentage of total 1 73.72090876615458 34.0912759815077 2 13.62708597733446 12.60333755110532 3 4.972527965491865 6.8984427634392524 4 2.311636539368903 4.275945077266489 5 1.32515623653597 3.0640063812604796 6 0.8337562878625786 2.313358545348533 7 0.5662277505606275 1.8329126410781615 8 0.4151528684702341 1.5358563805476073 9 0.30350541016847066 1.2631691857569551 >10 1.7124515547092864 14.568881540346407 >50 0.1311727668439881 4.182398315153284 >100 0.07025621476228257 6.172321428821785 >500 0.004859925178422981 1.5348272688823872 >1k 0.0049703780233871404 3.916008421267758 >5k 1.1045284496415868E-4 0.39789358795945945 >10k+ 2.2090568992831736E-4 1.349364930258408 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14901 0.7576377727757833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11517 0.5855791040238035 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7790 0.39608068249938605 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3442 0.17500766484761063 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3400 0.17287218491629172 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3095 0.15736453303409498 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3089 0.15705946447247798 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2712 0.13789098985087742 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2679 0.13621311276198397 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2480 0.12609500546835398 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2361 0.12004447899628375 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2340 0.1189767390306243 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 0.11536676105148999 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2118 0.10768920225079584 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 1980 0.10067262533360519 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.084476026949757E-5 2 0.0 0.0 0.0 0.0 5.084476026949757E-5 3 0.0 0.0 0.0 0.0 5.084476026949757E-5 4 0.0 0.0 0.0 0.0 5.084476026949757E-5 5 0.0 0.0 0.0 0.0 5.084476026949757E-5 6 0.0 5.084476026949757E-5 0.0 0.0 5.084476026949757E-5 7 0.0 5.084476026949757E-5 0.0 0.0 5.084476026949757E-5 8 5.084476026949757E-5 5.084476026949757E-5 0.0 0.0 1.525342808084927E-4 9 5.084476026949757E-5 5.084476026949757E-5 0.0 0.0 2.0337904107799027E-4 10-11 5.084476026949757E-5 5.084476026949757E-5 0.0 5.084476026949757E-5 2.0337904107799027E-4 12-13 5.084476026949757E-5 5.084476026949757E-5 0.0 5.084476026949757E-5 2.2880142121273905E-4 14-15 1.525342808084927E-4 5.084476026949757E-5 0.0 5.084476026949757E-5 3.050685616169854E-4 16-17 1.779566609432415E-4 5.084476026949757E-5 0.0 5.084476026949757E-5 3.55913321886483E-4 18-19 2.2880142121273905E-4 5.084476026949757E-5 0.0 7.626714040424636E-5 3.8133570202123174E-4 20-21 2.5422380134748786E-4 5.084476026949757E-5 0.0 1.525342808084927E-4 4.3218046229072935E-4 22-23 2.5422380134748786E-4 5.084476026949757E-5 0.0 2.796461814822366E-4 4.830252225602269E-4 24-25 3.55913321886483E-4 5.084476026949757E-5 0.0 7.880937841772122E-4 5.084476026949757E-4 26-27 3.55913321886483E-4 5.084476026949757E-5 0.0 0.0016270323286239222 5.338699828297245E-4 28-29 4.576028424254781E-4 5.084476026949757E-5 0.0 0.006050526472070211 5.592923629644732E-4 30-31 5.592923629644732E-4 5.084476026949757E-5 0.0 0.017643131813515657 5.592923629644732E-4 32-33 6.101371232339707E-4 5.084476026949757E-5 0.0 0.04255706434556947 7.11826643772966E-4 34-35 8.135161643119611E-4 5.084476026949757E-5 0.0 0.07713150132882782 7.626714040424635E-4 36-37 0.0010168952053899514 5.084476026949757E-5 0.0 0.13036596533099176 7.626714040424635E-4 38-39 0.0010168952053899514 5.084476026949757E-5 0.0 0.21161589224164887 7.626714040424635E-4 40-41 0.0010168952053899514 5.084476026949757E-5 0.0 0.2995519051277449 8.135161643119611E-4 42-43 0.0010168952053899514 5.084476026949757E-5 0.0 0.3891912174828691 8.135161643119611E-4 44-45 0.001067739965659449 5.084476026949757E-5 0.0 0.4917450989464457 8.135161643119611E-4 46-47 0.0011185847259289465 5.084476026949757E-5 0.0 0.611128596059226 9.40628064985705E-4 48-49 0.001169429486198444 5.084476026949757E-5 0.0 0.7370710672467715 9.660504451204538E-4 50-51 0.001169429486198444 5.084476026949757E-5 0.0 0.8734875590498334 9.660504451204538E-4 52-53 0.001194851866333193 5.084476026949757E-5 0.0 1.0020739577713926 0.001067739965659449 54-55 0.0012202742464679417 5.084476026949757E-5 0.0 1.1340923778111431 0.0011185847259289465 56-57 0.0013728085272764344 5.084476026949757E-5 0.0 1.2901603694583659 0.0011440071060636952 58-59 0.0013728085272764344 5.084476026949757E-5 0.0 1.4501942524066096 0.001169429486198444 60-61 0.0013728085272764344 5.084476026949757E-5 0.0 1.6226342568606107 0.001169429486198444 62-63 0.0013728085272764344 5.084476026949757E-5 0.0 1.7898372510068534 0.0012711190067374392 64-65 0.0013728085272764344 5.084476026949757E-5 0.0 1.9633449954265139 0.0012711190067374392 66-67 0.001423653287545932 5.084476026949757E-5 0.0 2.160978578594051 0.0012711190067374392 68-69 0.001423653287545932 5.084476026949757E-5 0.0 2.3802211848761243 0.0012711190067374392 70-71 0.0014490756676806807 5.084476026949757E-5 0.0 2.6006332206443963 0.0013473861471416855 72-73 0.0014744980478154294 5.084476026949757E-5 0.0 2.8363241068736524 0.001525342808084927 74-75 0.0014744980478154294 5.084476026949757E-5 0.0 3.0661170009116465 0.001525342808084927 76-77 0.0014744980478154294 5.084476026949757E-5 0.0 3.305799200822058 0.001525342808084927 78-79 0.0014999204279501782 5.084476026949757E-5 0.0 3.5776407116029265 0.001525342808084927 80-81 0.00188125612997141 5.084476026949757E-5 0.0 3.8539565612875113 0.001550765188219676 82-83 0.0019321008902409076 5.084476026949757E-5 0.0 4.1492120841724836 0.001652454708758671 84-85 0.002033790410779903 5.084476026949757E-5 0.0 4.450365599248718 0.0016778770888934197 86-87 0.002135479931318898 5.084476026949757E-5 0.0 4.757162882714866 0.0017032994690281684 88-89 0.002135479931318898 5.084476026949757E-5 0.0 5.074357919656126 0.0017287218491629172 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5485 0.0 53.113888 1 GTATCAA 10040 0.0 43.525345 1 TATCAAC 12910 0.0 33.78912 2 TCAACGC 13275 0.0 32.932507 4 ATCAACG 13270 0.0 32.837486 3 CAACGCA 13400 0.0 32.589836 5 GTGGTAT 2510 0.0 32.53698 1 TGGTATC 2510 0.0 32.183674 2 AACGCAG 13890 0.0 31.440163 6 TAAGGTG 1265 0.0 30.80313 5 CGCAGAG 15365 0.0 28.158699 8 ACGCAGA 15420 0.0 28.150763 7 AGGTAAG 1190 0.0 27.5526 2 GTAAGGT 1330 0.0 26.796688 4 GCAGAGT 16555 0.0 25.701195 9 TACATGG 9185 0.0 24.987823 2 GTACATG 9510 0.0 24.908985 1 GGTAAGG 1405 0.0 24.689827 3 AAGGTAA 1310 0.0 24.061766 1 TAAGGTA 635 0.0 23.946817 4 >>END_MODULE