FastQCFastQC Report
Thu 26 May 2016
SRR938025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966771
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143670.7304866707918715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115920.5893924610440158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76650.38972508746569884No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35440.18019383039509937No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34300.17439752772437667No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31670.1610253557734988No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30440.15477145026035058No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29920.15212752272633673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.1378401450906079No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25890.1316370843377292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.12426459409865206No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22820.11602774293499345No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22740.11562098485283746No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22460.11419733156529153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56650.053.502061
GTATCAA100300.043.3381041
TATCAAC130900.033.0264972
ATCAACG133450.032.4608423
TCAACGC134400.032.3012544
CAACGCA137300.031.4806215
AACGCAG141650.030.54746
TAAGGTG12900.028.3513345
AGGTAAG11500.027.2650872
CGCAGAG159950.026.7851928
ACGCAGA161050.026.6317377
GTGGTAT26200.025.9289261
TGGTATC26000.025.2154392
GTAAGGT13500.024.9802654
GTACATG94250.024.7989621
TACATGG94250.024.4971562
GCAGAGT174600.024.2106919
GGTAAGG14350.023.8321973
GTATAGG3200.023.7530691
GTATACG1000.001652818423.7530691
Produced by FastQC (version 0.11.5)