##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938025_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1966771 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.528277567647685 34.0 31.0 34.0 31.0 34.0 2 32.744875229500536 34.0 31.0 34.0 31.0 34.0 3 32.792497448864154 34.0 31.0 34.0 31.0 34.0 4 36.25293997115068 37.0 35.0 37.0 35.0 37.0 5 36.21721898482335 37.0 37.0 37.0 35.0 37.0 6 36.260828535706494 37.0 37.0 37.0 35.0 37.0 7 36.225277879326065 37.0 37.0 37.0 35.0 37.0 8 36.21637241956486 37.0 37.0 37.0 35.0 37.0 9 37.92950068920072 39.0 38.0 39.0 35.0 39.0 10-11 37.916031149533936 39.0 38.0 39.0 35.0 39.0 12-13 37.86757405920669 39.0 38.0 39.0 35.0 39.0 14-15 39.18236693544902 40.5 39.0 41.0 36.0 41.0 16-17 39.14276547701792 40.0 38.5 41.0 36.0 41.0 18-19 39.18260031289866 40.0 39.0 41.0 36.0 41.0 20-21 39.115597850486914 40.0 39.0 41.0 36.0 41.0 22-23 38.977743977311036 40.0 38.5 41.0 35.0 41.0 24-25 38.9214212025701 40.0 38.0 41.0 35.0 41.0 26-27 38.866696224420636 40.0 38.0 41.0 35.0 41.0 28-29 38.80109199291631 40.0 38.0 41.0 35.0 41.0 30-31 38.57605893111094 40.0 38.0 41.0 34.5 41.0 32-33 38.36134837253549 40.0 38.0 41.0 34.0 41.0 34-35 38.14388940044367 40.0 38.0 41.0 34.0 41.0 36-37 37.991926614740606 40.0 37.0 41.0 33.5 41.0 38-39 37.95556650977669 40.0 37.0 41.0 33.0 41.0 40-41 37.873880334822914 40.0 37.0 41.0 33.0 41.0 42-43 37.690730135841946 40.0 37.0 41.0 33.0 41.0 44-45 37.44997282347563 40.0 37.0 41.0 33.0 41.0 46-47 37.06501697452322 40.0 36.5 41.0 31.5 41.0 48-49 36.6496879911286 40.0 36.0 41.0 31.0 41.0 50-51 36.307776045101335 39.5 35.0 41.0 30.0 41.0 52-53 35.87053652916379 39.0 35.0 41.0 29.0 41.0 54-55 35.61683795418989 39.0 35.0 40.5 28.0 41.0 56-57 35.33987408803567 38.0 35.0 40.0 28.0 41.0 58-59 34.970025996925926 38.0 34.5 40.0 27.5 41.0 60-61 34.582931617356564 37.0 34.0 40.0 26.0 41.0 62-63 34.19214895887727 37.0 34.0 40.0 26.0 41.0 64-65 33.770921729067595 36.0 34.0 39.0 25.5 41.0 66-67 33.34539125297251 35.5 33.0 39.0 25.0 40.5 68-69 32.881084528905504 35.0 33.0 38.5 23.0 40.0 70-71 32.36444964868813 35.0 32.5 37.5 22.0 40.0 72-73 31.96395182764033 35.0 32.0 37.0 20.5 39.0 74-75 31.54676802739109 35.0 31.5 36.0 20.0 39.0 76-77 30.278347860528754 33.5 30.0 35.0 18.5 37.0 78-79 30.993929135623823 34.5 31.0 35.0 19.0 37.0 80-81 30.862576019272197 35.0 31.0 35.0 18.0 37.0 82-83 30.53008459042766 34.0 31.0 35.0 16.0 36.5 84-85 30.19471128057105 34.0 31.0 35.0 11.0 36.0 86-87 29.808758620093542 34.0 30.5 35.0 7.0 36.0 88-89 29.366949177102978 34.0 30.0 35.0 2.0 35.0 90-91 29.05236374748255 34.0 29.5 35.0 2.0 35.0 92-93 28.670409010505036 34.0 29.0 35.0 2.0 35.0 94-95 28.291112437594414 34.0 29.0 35.0 2.0 35.0 96-97 27.740865611705686 34.0 28.0 35.0 2.0 35.0 98-99 27.073880233133394 33.0 27.0 35.0 2.0 35.0 100-101 24.654693912000937 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05531116476636555 1101 2 0.07671980000424128 1101 3 0.05003685336441066 1101 4 -0.005693387335064415 1101 5 0.09725724185100404 1101 6 0.1100089105552371 1101 7 0.050566477794191655 1101 8 0.0065306140413738945 1101 9 -0.02680185269750268 1101 10-11 0.044750865544870067 1101 12-13 0.06258983780570304 1101 14-15 0.09380196456113055 1101 16-17 0.11902016786191183 1101 18-19 0.17483339926214825 1101 20-21 0.15861891141457107 1101 22-23 0.13370469381758454 1101 24-25 0.16036683603739021 1101 26-27 0.1145205761597623 1101 28-29 0.09821411051734685 1101 30-31 0.09808653284428459 1101 32-33 0.0298195832001511 1101 34-35 0.003436043468234118 1101 36-37 0.06721249746985336 1101 38-39 0.16012627638701815 1101 40-41 0.20805392236660936 1101 42-43 0.23463755508509365 1101 44-45 0.2614177285295938 1101 46-47 0.23720876079753594 1101 48-49 0.17490190959912155 1101 50-51 0.023248848934848354 1101 52-53 0.06683876336595063 1101 54-55 0.0963606277152067 1101 56-57 0.12525758277082133 1101 58-59 0.14992378828221575 1101 60-61 0.08208817122002898 1101 62-63 0.13663607519866616 1101 64-65 0.1547082074654753 1101 66-67 0.11855677796682329 1101 68-69 0.16116891658037247 1101 70-71 0.10695105238941238 1101 72-73 0.05456680533060876 1101 74-75 0.24148063354375893 1101 76-77 0.19338851109167976 1101 78-79 0.17913845356032 1101 80-81 0.3309783470150798 1101 82-83 0.39866405998176546 1101 84-85 0.38914879525725254 1101 86-87 0.4815658298219496 1101 88-89 0.40245227468049904 1101 90-91 0.41624195932203634 1101 92-93 0.40980806098971456 1101 94-95 0.4236563469036163 1101 96-97 0.4103825933664993 1101 98-99 0.5569437914406841 1101 100-101 0.6350229687149636 1102 1 0.16071597835327367 1102 2 0.10850133059879141 1102 3 0.09393934239370338 1102 4 0.02068244694719823 1102 5 0.06097580508161826 1102 6 0.05532805991550305 1102 7 0.04532052630023742 1102 8 0.061730564153158696 1102 9 0.13333022579899279 1102 10-11 0.06220106488418509 1102 12-13 0.14727210365353471 1102 14-15 0.228007544467566 1102 16-17 0.16709082917413554 1102 18-19 0.20799084244524835 1102 20-21 0.13288333778117334 1102 22-23 0.20674637468616197 1102 24-25 0.19711526300652338 1102 26-27 0.04512426635049138 1102 28-29 0.09547652284634722 1102 30-31 0.17769512102185558 1102 32-33 0.1428492931736045 1102 34-35 0.14813651623885704 1102 36-37 0.21585063729981613 1102 38-39 0.19325168518141567 1102 40-41 0.20570145923534966 1102 42-43 0.06392031793580344 1102 44-45 0.24460309146213177 1102 46-47 0.23129535501807652 1102 48-49 0.12254632845402824 1102 50-51 0.259596433157526 1102 52-53 0.24461296278386868 1102 54-55 0.32678469752910644 1102 56-57 0.27982422013669606 1102 58-59 0.3935505822435843 1102 60-61 0.23441012337574563 1102 62-63 0.14017409546205073 1102 64-65 0.1587895309612719 1102 66-67 0.1256248454901865 1102 68-69 0.3828833344187146 1102 70-71 0.5900517021607143 1102 72-73 0.5033789844350238 1102 74-75 0.5269968755483347 1102 76-77 0.5223552013071817 1102 78-79 0.5884949439125577 1102 80-81 0.6241619131743477 1102 82-83 0.6125864331120603 1102 84-85 0.5618168554910135 1102 86-87 0.5614103334754361 1102 88-89 0.4638264616833112 1102 90-91 0.382902845561226 1102 92-93 0.3577420707184835 1102 94-95 0.3022676547296044 1102 96-97 0.4442052128341203 1102 98-99 0.30127491482831914 1102 100-101 0.6589340059429105 1103 1 0.14443938582644478 1103 2 0.204434580491764 1103 3 0.16244818252094007 1103 4 0.08724343702618853 1103 5 0.09487542649347347 1103 6 0.03143201646547311 1103 7 0.09511332879265666 1103 8 0.11910303215928053 1103 9 0.16903054574454046 1103 10-11 0.14251172898169528 1103 12-13 0.16074191729159537 1103 14-15 0.22910518576504302 1103 16-17 0.17587018828569256 1103 18-19 0.2513535325057319 1103 20-21 0.1995815330223465 1103 22-23 0.17669531038558262 1103 24-25 0.15781817854128377 1103 26-27 0.1054967177339492 1103 28-29 0.13133802820088647 1103 30-31 0.15666825960288833 1103 32-33 0.1629049545964989 1103 34-35 0.26892215817508003 1103 36-37 0.27179260183248033 1103 38-39 0.28656672812650186 1103 40-41 0.3643619934988962 1103 42-43 0.3381026937079028 1103 44-45 0.36454342969806675 1103 46-47 0.37487653782739727 1103 48-49 0.35136911361254164 1103 50-51 0.3990377533573195 1103 52-53 0.4216861028091543 1103 54-55 0.4491109340718964 1103 56-57 0.5503051903120806 1103 58-59 0.6479820909798804 1103 60-61 0.6128043842486832 1103 62-63 0.6473082188900179 1103 64-65 0.49962268648651786 1103 66-67 0.6553512226989255 1103 68-69 0.6804478133511154 1103 70-71 0.733201649281547 1103 72-73 0.529206800267815 1103 74-75 0.42615762141031155 1103 76-77 0.4655742006387342 1103 78-79 0.5453540711351259 1103 80-81 0.6470521487720973 1103 82-83 0.6100645559056375 1103 84-85 0.594281204630331 1103 86-87 0.4972000888414918 1103 88-89 0.4656911983761134 1103 90-91 0.5148025143885704 1103 92-93 0.6571256421238942 1103 94-95 0.4878905702534624 1103 96-97 0.5639118799149934 1103 98-99 0.6963317684509889 1103 100-101 0.7836160292326397 1104 1 0.09646031516592046 1104 2 0.06852988259110049 1104 3 0.09195567100019986 1104 4 0.01998313236669702 1104 5 0.06626559546428723 1104 6 -0.013010827876563269 1104 7 0.03702961143474681 1104 8 -0.0016406954392849116 1104 9 -0.060464141915474556 1104 10-11 -0.007505872144768944 1104 12-13 0.08910533255017583 1104 14-15 0.12000220317415966 1104 16-17 0.12317081552583176 1104 18-19 0.031175441107883728 1104 20-21 0.0606566418293113 1104 22-23 0.10022241612035288 1104 24-25 -0.00920051065482852 1104 26-27 0.032716059771836115 1104 28-29 -0.04828546770284703 1104 30-31 0.10386888044261866 1104 32-33 0.13826337753556572 1104 34-35 0.22697947384327932 1104 36-37 0.225653648939506 1104 38-39 0.13645712111164698 1104 40-41 0.10922166451783966 1104 42-43 0.24008442053841605 1104 44-45 0.29867121387624707 1104 46-47 0.4412007825790667 1104 48-49 0.4567438575961589 1104 50-51 0.5005002469569035 1104 52-53 0.5513063985080819 1104 54-55 0.43462600703656307 1104 56-57 0.48208882446466816 1104 58-59 0.4898479703961627 1104 60-61 0.45454225547243965 1104 62-63 0.4634764536570444 1104 64-65 0.4161453439365985 1104 66-67 0.47300755270404693 1104 68-69 0.3995338348564914 1104 70-71 0.48791395086885103 1104 72-73 0.42019582356530805 1104 74-75 0.30011355459003397 1104 76-77 0.4627820219392973 1104 78-79 0.41380565203630226 1104 80-81 0.3810071218903808 1104 82-83 0.3418553905190542 1104 84-85 0.45182408737482405 1104 86-87 0.3310214534088267 1104 88-89 0.3309825770243435 1104 90-91 0.33816320947305556 1104 92-93 0.3913766341873881 1104 94-95 0.4609408454710753 1104 96-97 0.4763382752042915 1104 98-99 0.9346550211391573 1104 100-101 0.6648524283621811 1105 1 0.07947799995129401 1105 2 0.0895127058067473 1105 3 0.10827123488132884 1105 4 0.09924319713682195 1105 5 0.10477842248993596 1105 6 0.0986660517272 1105 7 -0.01959662681773011 1105 8 0.027124862091831403 1105 9 0.026090499943812517 1105 10-11 0.05174371510440068 1105 12-13 0.025716330772169727 1105 14-15 0.06668987779950442 1105 16-17 0.1452966410274925 1105 18-19 0.03764650092499977 1105 20-21 0.17900698790500513 1105 22-23 0.1664550329605845 1105 24-25 0.12174468873557487 1105 26-27 0.03120396299764394 1105 28-29 0.015514300521687119 1105 30-31 0.15161899156784386 1105 32-33 0.2187418509925152 1105 34-35 0.1529403101399538 1105 36-37 0.15749023136266516 1105 38-39 0.02208743924728651 1105 40-41 0.05672491889934861 1105 42-43 0.18211475772409358 1105 44-45 0.23329584875051523 1105 46-47 0.19797942113634548 1105 48-49 0.23541813369470788 1105 50-51 0.2959028377620925 1105 52-53 0.3538785505273907 1105 54-55 0.3482598811793096 1105 56-57 0.20253149972501916 1105 58-59 0.18118774370071122 1105 60-61 0.2525463671056585 1105 62-63 0.37401866536647077 1105 64-65 0.37636251437093904 1105 66-67 0.21259154749703413 1105 68-69 0.41117023312929035 1105 70-71 0.38205527040148723 1105 72-73 0.299908723616106 1105 74-75 0.39569116762026013 1105 76-77 0.38585558683642773 1105 78-79 0.5321639356278709 1105 80-81 0.513625152751441 1105 82-83 0.5490086798256293 1105 84-85 0.6316564470395427 1105 86-87 0.6873008222175621 1105 88-89 0.5945537447916784 1105 90-91 0.5935547671311703 1105 92-93 0.6846301425694037 1105 94-95 0.6030084889813594 1105 96-97 0.8470996417270271 1105 98-99 0.8099705373951949 1105 100-101 0.5970997772318789 1106 1 -0.17526419484821076 1106 2 -0.03858131521388941 1106 3 0.007783276581434961 1106 4 4.489663750462114E-4 1106 5 -0.08166923720990837 1106 6 -0.12567506645282123 1106 7 -0.002257606049091976 1106 8 -0.13228009919716754 1106 9 -0.05910690291037213 1106 10-11 0.0034808398674286423 1106 12-13 -0.11441103075409842 1106 14-15 -0.15302668574120304 1106 16-17 -0.14210178378167626 1106 18-19 -0.04446451358629133 1106 20-21 0.017168535104509886 1106 22-23 -0.1379968535465821 1106 24-25 -0.03036936915453481 1106 26-27 -0.07470137919832354 1106 28-29 0.07156141667795879 1106 30-31 0.04464955717106989 1106 32-33 -0.024438533171057486 1106 34-35 0.11813493615328241 1106 36-37 0.12925841022029516 1106 38-39 0.13778793006481038 1106 40-41 0.18988134372401788 1106 42-43 0.09662743426914489 1106 44-45 0.20088883172698502 1106 46-47 0.29535603344864825 1106 48-49 0.14477221046538347 1106 50-51 0.2474601705604087 1106 52-53 0.07186637313522937 1106 54-55 -0.16210190289974236 1106 56-57 -0.02566436302441133 1106 58-59 0.18938754151955806 1106 60-61 0.07571188329759337 1106 62-63 0.021272524836028595 1106 64-65 -0.013516966858972523 1106 66-67 0.06959102799033445 1106 68-69 -0.24969147922683277 1106 70-71 -0.33150923372161145 1106 72-73 -0.05348195414629231 1106 74-75 -0.12821050216732388 1106 76-77 -0.14655227826241557 1106 78-79 -0.05349094327666393 1106 80-81 -0.13561489908292046 1106 82-83 -0.22051159062800352 1106 84-85 -0.1911657198073975 1106 86-87 -0.05100397701635728 1106 88-89 0.03421888104588078 1106 90-91 -0.1816093233250271 1106 92-93 0.07426973964155437 1106 94-95 0.050762446343043166 1106 96-97 0.24743516454848802 1106 98-99 0.25526507637176366 1106 100-101 0.28285516517544806 1107 1 0.10678818527508582 1107 2 0.09875456982279474 1107 3 0.05815484615045108 1107 4 0.049280246925754057 1107 5 0.04803610938554215 1107 6 0.03253030728841111 1107 7 0.06285599436127853 1107 8 -0.008946288472522212 1107 9 -0.015455786095529334 1107 10-11 0.037284005814264276 1107 12-13 0.048817248409363856 1107 14-15 0.13921483962699455 1107 16-17 0.09127879725036081 1107 18-19 0.041333703403971356 1107 20-21 0.08084188805769799 1107 22-23 0.16341713738859198 1107 24-25 0.1055578604968943 1107 26-27 0.07124673866304221 1107 28-29 0.12464838333357164 1107 30-31 0.10164481414800264 1107 32-33 0.1375887792281958 1107 34-35 0.11760734855197796 1107 36-37 0.11395205136805231 1107 38-39 0.10734686261784532 1107 40-41 0.12980936996368797 1107 42-43 0.06114626127189382 1107 44-45 0.12751776575899498 1107 46-47 0.22812354107286836 1107 48-49 0.2512615964652838 1107 50-51 0.12093520954736903 1107 52-53 0.07379524463834031 1107 54-55 0.15136083624053498 1107 56-57 0.16357589210847578 1107 58-59 0.08490787932539234 1107 60-61 0.04810731587965478 1107 62-63 0.2351278013204805 1107 64-65 0.21968775163928456 1107 66-67 0.03685579108255155 1107 68-69 0.054530461864949586 1107 70-71 0.1106505968887177 1107 72-73 -0.03800687022838645 1107 74-75 0.1343741411839261 1107 76-77 0.12543112349700536 1107 78-79 0.16951386527069445 1107 80-81 0.27677689222031887 1107 82-83 0.18540991032771004 1107 84-85 0.24307807363755174 1107 86-87 0.3274089028139535 1107 88-89 0.31051752581620207 1107 90-91 0.21489282587192804 1107 92-93 0.3703347247675417 1107 94-95 0.283875079801998 1107 96-97 0.4015843502686849 1107 98-99 0.4262675793999229 1107 100-101 0.5439239741122108 1108 1 0.01881137289749546 1108 2 0.013805328613024415 1108 3 0.008954269329187525 1108 4 -0.011602071170074169 1108 5 -0.042322682325711014 1108 6 0.024668896186632594 1108 7 -0.024826483674715405 1108 8 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-0.6954733953987287 2316 54-55 -0.8701735001318909 2316 56-57 -0.8197984963238625 2316 58-59 -0.6343399776325143 2316 60-61 -0.5867603878967387 2316 62-63 -0.6051559388160754 2316 64-65 -0.6941817169953026 2316 66-67 -0.5305486748945185 2316 68-69 -0.5827690325270254 2316 70-71 -0.7517253860487152 2316 72-73 -0.5984813717751933 2316 74-75 -0.46145118555333653 2316 76-77 -0.43153921461984623 2316 78-79 -0.5374992314762395 2316 80-81 -0.6504388906544207 2316 82-83 -0.6081894123483274 2316 84-85 -0.5189420416574357 2316 86-87 -0.767936879924509 2316 88-89 -0.7891731129398138 2316 90-91 -0.9090646131075886 2316 92-93 -1.078409432120857 2316 94-95 -1.005485005783406 2316 96-97 -0.8349296459426654 2316 98-99 -0.8146449340579736 2316 100-101 -0.8300232436808272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 14.0 5 35.0 6 71.0 7 196.0 8 493.0 9 820.0 10 1497.0 11 2374.0 12 3699.0 13 5305.0 14 7429.0 15 9948.0 16 13563.0 17 20084.0 18 20215.0 19 13672.0 20 11238.0 21 10994.0 22 11087.0 23 11672.0 24 13248.0 25 15076.0 26 17803.0 27 21412.0 28 26063.0 29 32352.0 30 39269.0 31 48176.0 32 60792.0 33 79572.0 34 113827.0 35 182293.0 36 304471.0 37 477055.0 38 356790.0 39 34160.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39667861688015 18.043381766357143 14.738065590757643 22.821874026005062 2 16.34180598469146 19.659707376210537 38.787704349488735 25.210782289609273 3 19.703473643853116 23.437409426645882 29.522362529352126 27.336754400148884 4 13.466641515458585 16.18444648614404 34.91962206072797 35.42928993766941 5 13.971791312659843 37.049477061374745 33.20154364770664 15.77718797825877 6 33.60611561087468 34.96529843347214 16.032886407663327 15.395699547989853 7 29.688611434681516 33.036382985106044 18.90484453960324 18.3701610406092 8 28.01129776891944 33.438599524090385 18.578881002257518 19.971221704732656 9 27.32055866586467 16.087142438033776 19.15381513832161 37.43848375777994 10-11 25.946132010284877 25.982867349579593 25.5406196247555 22.530381015380033 12-13 27.03888759799692 23.342219302603098 26.558023277748145 23.060869821651835 14-15 23.506168232092094 24.41489121000869 25.258151559078307 26.82078899882091 16-17 22.633414049739102 27.78891706548198 26.234611449018374 23.343057435760546 18-19 22.789663870374337 25.591769453586615 29.237211653008917 22.38135502303013 20-21 23.05807335983701 24.53562209326861 30.003543879790783 22.402760667103593 22-23 23.154195379126495 24.487370415772858 29.889982107728862 22.46845209737178 24-25 22.944093185566466 24.799043452682255 29.73208590315594 22.524777458595338 26-27 21.961906142288097 25.345396430655626 29.746443560393676 22.946253866662598 28-29 22.486807055829072 25.708229376983898 29.447073401021267 22.35789016616576 30-31 22.296856699853898 25.176653808870935 29.348772518196707 23.177716973078454 32-33 22.03563522175027 25.207218768717315 29.421353473374 23.33579253615842 34-35 23.0450097628414 25.339652545064308 29.22045510427894 22.394882587815353 36-37 23.166470186096802 24.791153060863895 29.082293791375008 22.9600829616643 38-39 22.508525793003002 25.206775271996445 29.04394152521428 23.240757409786276 40-41 23.020398117222047 25.03226616817259 29.172862245582916 22.774473469022443 42-43 22.594372039134715 25.091331880565942 28.960183139203032 23.354112941096307 44-45 22.52771893714668 25.408347765248084 29.24738115407183 22.8165521435334 46-47 23.236080865540522 25.798199180280772 28.197715951679175 22.768004002499527 48-49 22.87154935678836 25.83521416575697 28.029190993765923 23.264045483688747 50-51 23.05649717226866 26.176331662405026 28.003336433168887 22.763834732157427 52-53 23.521243703512003 25.737541381279268 27.899994457921128 22.841220457287605 54-55 22.810637333985504 26.211109478429368 27.95589827183744 23.02235491574769 56-57 22.81127289348887 26.342441494205477 27.810711058887893 23.03557455341776 58-59 22.867354664066127 25.745854499583327 27.678972285029623 23.707818551320923 60-61 22.393659455015353 26.21456692212769 28.098517824393383 23.293255798463573 62-63 22.575505740119212 26.261369523955764 28.28173691802452 22.88138781790051 64-65 22.19856810986129 26.37828705019547 28.015717132294508 23.40742770764873 66-67 22.199229091744794 26.292156026298947 28.04528336039122 23.463331521565042 68-69 22.82107569908492 25.8966044537547 28.203384487490258 23.07893535967012 70-71 22.591140503902082 26.27662295203661 27.56101244120439 23.571224102856917 72-73 22.58557300265257 26.42178474260603 27.390479115260497 23.602163139480904 74-75 22.45157163696231 26.961527295246878 27.333634673279196 23.253266394511613 76-77 22.687262523191567 26.380956400109625 27.6013323360981 23.33044874060071 78-79 22.703202355536053 26.57553929766099 27.653982085357164 23.06727626144579 80-81 22.93965591316935 26.648882864349737 27.26270623270325 23.14875498977766 82-83 22.781458233179723 26.74490999331137 27.362597720476284 23.111034053032622 84-85 22.549424880588106 26.96115668610002 27.25237730599444 23.237041127317433 86-87 22.96593438050238 27.00226209258563 26.812661210323768 23.219142316588222 88-89 22.692511071452177 27.133726871977913 26.654336806032227 23.519425250537683 90-91 22.520783489439893 26.988347111030777 26.8869115306324 23.603957868896927 92-93 22.730953201128248 26.58763682031557 27.08825522081283 23.593154757743353 94-95 22.487417192952304 26.309977114773403 27.364904200844936 23.837701491429353 96-97 22.80173950093834 25.874491743065153 27.301500784788875 24.02226797120763 98-99 22.430165992888853 26.453054270171766 26.90386933710127 24.212910399838112 100-101 22.415954882393528 26.106343849894063 27.351862519835812 24.125838747876596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1965.0 1 1306.0 2 963.5 3 1272.5 4 1019.5 5 938.5 6 1859.0 7 4120.0 8 6560.5 9 7158.5 10 5393.5 11 3581.5 12 3182.0 13 3227.5 14 3351.0 15 3451.5 16 3511.5 17 3623.0 18 3763.0 19 3929.0 20 4255.0 21 4839.0 22 5529.5 23 6444.0 24 7551.5 25 8994.0 26 10909.5 27 12931.0 28 14833.0 29 16771.5 30 18639.5 31 22003.0 32 28402.0 33 29674.5 34 27008.5 35 27846.5 36 30126.5 37 33121.5 38 36573.5 39 39300.0 40 42073.5 41 46593.5 42 49338.0 43 51899.5 44 55785.5 45 61021.0 46 64752.5 47 69075.5 48 71930.5 49 70663.5 50 71885.0 51 72395.5 52 73124.5 53 77353.0 54 82018.5 55 78892.5 56 73308.5 57 68869.0 58 65307.0 59 59113.0 60 47119.0 61 39019.5 62 33228.5 63 24408.0 64 17114.5 65 13346.5 66 9902.0 67 7640.5 68 6011.0 69 4462.0 70 3299.5 71 2111.5 72 1384.5 73 1116.0 74 833.5 75 539.5 76 357.0 77 222.5 78 131.5 79 73.0 80 44.0 81 25.0 82 15.0 83 7.5 84 4.0 85 3.0 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014592446197345801 3 0.006762353115843176 4 0.0 5 5.084476026949757E-5 6 5.084476026949757E-5 7 0.0 8 5.592923629644732E-4 9 7.11826643772966E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.3218046229072935E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005821725050857472 26-27 0.022549651179522172 28-29 0.0 30-31 2.5422380134748784E-5 32-33 4.576028424254781E-4 34-35 0.01965149984416081 36-37 0.004042158441425057 38-39 0.019575232703756565 40-41 0.016016099484891733 42-43 0.009126634468374813 44-45 0.003304909417517342 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.779566609432415E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.5422380134748784E-5 84-85 0.002491393253205381 86-87 4.0675808215598054E-4 88-89 5.084476026949757E-5 90-91 2.796461814822366E-4 92-93 1.779566609432415E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1966771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.11594194427009 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64954130960808 31.60887004748252 2 14.456589733343048 12.75532146776586 3 5.4328133326771315 7.190210327353225 4 2.5880713513403366 4.567008219334357 5 1.4958626374665533 3.2995694635538606 6 0.9193086856684735 2.433370116348817 7 0.6097957360425026 1.8831199302380146 8 0.4388354799422226 1.5487712444973596 9 0.3376476893596469 1.3406081275266413 >10 1.837767707661337 15.01079050117433 >50 0.1457472285036862 4.454342464766439 >100 0.07659952539693146 6.4154022490509774 >500 0.00611351412601289 1.881868434399905 >1k 0.004960020894689703 3.899708109618515 >5k 1.1534932313231868E-4 0.3900522308665334 >10k+ 2.3069864626463737E-4 1.320987066022745 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14367 0.7304866707918715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11592 0.5893924610440158 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7665 0.38972508746569884 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3544 0.18019383039509937 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3430 0.17439752772437667 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3167 0.1610253557734988 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3044 0.15477145026035058 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2992 0.15212752272633673 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2711 0.1378401450906079 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2589 0.1316370843377292 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.12426459409865206 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2282 0.11602774293499345 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2274 0.11562098485283746 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2246 0.11419733156529153 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.084476026949757E-5 2 1.0168952053899514E-4 0.0 0.0 0.0 5.084476026949757E-5 3 1.0168952053899514E-4 0.0 0.0 0.0 5.084476026949757E-5 4 1.0168952053899514E-4 0.0 0.0 0.0 5.084476026949757E-5 5 1.0168952053899514E-4 0.0 0.0 0.0 5.084476026949757E-5 6 1.525342808084927E-4 0.0 0.0 0.0 5.084476026949757E-5 7 1.525342808084927E-4 0.0 0.0 0.0 5.084476026949757E-5 8 1.525342808084927E-4 0.0 0.0 0.0 5.084476026949757E-5 9 1.525342808084927E-4 0.0 0.0 5.084476026949757E-5 1.0168952053899514E-4 10-11 1.525342808084927E-4 0.0 0.0 1.0168952053899514E-4 1.0168952053899514E-4 12-13 1.525342808084927E-4 0.0 0.0 1.0168952053899514E-4 1.0168952053899514E-4 14-15 1.779566609432415E-4 0.0 0.0 1.0168952053899514E-4 2.0337904107799027E-4 16-17 2.2880142121273905E-4 0.0 0.0 1.0168952053899514E-4 2.5422380134748786E-4 18-19 2.796461814822366E-4 0.0 0.0 1.2711190067374393E-4 3.050685616169854E-4 20-21 3.050685616169854E-4 0.0 0.0 1.779566609432415E-4 3.050685616169854E-4 22-23 3.050685616169854E-4 0.0 0.0 2.796461814822366E-4 3.8133570202123174E-4 24-25 4.0675808215598054E-4 0.0 0.0 7.880937841772122E-4 4.576028424254781E-4 26-27 4.576028424254781E-4 0.0 0.0 0.0016270323286239222 4.830252225602269E-4 28-29 6.101371232339707E-4 0.0 0.0 0.00607594885220496 5.084476026949757E-4 30-31 6.609818835034683E-4 0.0 0.0 0.017744821334054653 5.592923629644732E-4 32-33 6.609818835034683E-4 0.0 0.0 0.04276044338664745 7.372490239077147E-4 34-35 7.11826643772966E-4 0.0 0.0 0.07781790559246603 7.626714040424635E-4 36-37 7.626714040424635E-4 0.0 0.0 0.13148455005692072 7.626714040424635E-4 38-39 8.643609245814586E-4 0.0 0.0 0.21306496790932955 7.626714040424635E-4 40-41 8.643609245814586E-4 0.0 0.0 0.3018399193398723 7.626714040424635E-4 42-43 8.897833047162075E-4 0.0 0.0 0.39226732547917376 7.880937841772122E-4 44-45 0.0010168952053899514 0.0 0.0 0.4953296545454453 8.389385444467098E-4 46-47 0.001169429486198444 0.0 0.0 0.6144335054767434 9.40628064985705E-4 48-49 0.0012202742464679417 0.0 0.0 0.7408590018868491 0.001067739965659449 50-51 0.0012202742464679417 0.0 0.0 0.8777839412926061 0.001067739965659449 52-53 0.0012202742464679417 0.0 0.0 1.0071330114182078 0.0011440071060636954 54-55 0.0012202742464679417 0.0 0.0 1.1399649476222704 0.0012202742464679417 56-57 0.0012711190067374392 0.0 0.0 1.29633800783111 0.0012202742464679417 58-59 0.0012711190067374392 0.0 0.0 1.4566515369608357 0.0012711190067374392 60-61 0.0012711190067374392 0.0 0.0 1.6303626604215742 0.0012711190067374392 62-63 0.0012711190067374392 0.0 0.0 1.7982520588314552 0.0012711190067374392 64-65 0.001296541386872188 0.0 0.0 1.9717852256312505 0.001296541386872188 66-67 0.001423653287545932 0.0 0.0 2.170079790682291 0.0013473861471416855 68-69 0.001423653287545932 0.0 0.0 2.3898054221869245 0.0013728085272764344 70-71 0.0014999204279501784 0.0 0.0 2.6119716021844943 0.0014490756676806807 72-73 0.0016016099484891734 0.0 0.0 2.8494674774033175 0.0016270323286239222 74-75 0.0016778770888934197 0.0 0.0 3.080684024728858 0.0016778770888934197 76-77 0.0017032994690281684 0.0 0.0 3.322476282190453 0.001754144229297666 78-79 0.0017287218491629172 0.0 0.0 3.597648124768974 0.001779566609432415 80-81 0.001906678510106159 0.0 0.0 3.8763791005663597 0.0018304113697019124 82-83 0.002033790410779903 0.0 0.0 4.174710731447637 0.001906678510106159 84-85 0.002059212790914652 0.0 0.0 4.478635285958559 0.0019321008902409076 86-87 0.002135479931318898 0.0 0.0 4.790618734972195 0.0019575232703756564 88-89 0.0021863246915883954 0.0 0.0 5.114728659310108 0.001982945650510405 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5665 0.0 53.50206 1 GTATCAA 10030 0.0 43.338104 1 TATCAAC 13090 0.0 33.026497 2 ATCAACG 13345 0.0 32.460842 3 TCAACGC 13440 0.0 32.301254 4 CAACGCA 13730 0.0 31.480621 5 AACGCAG 14165 0.0 30.5474 6 TAAGGTG 1290 0.0 28.351334 5 AGGTAAG 1150 0.0 27.265087 2 CGCAGAG 15995 0.0 26.785192 8 ACGCAGA 16105 0.0 26.631737 7 GTGGTAT 2620 0.0 25.928926 1 TGGTATC 2600 0.0 25.215439 2 GTAAGGT 1350 0.0 24.980265 4 GTACATG 9425 0.0 24.798962 1 TACATGG 9425 0.0 24.497156 2 GCAGAGT 17460 0.0 24.210691 9 GGTAAGG 1435 0.0 23.832197 3 GTATAGG 320 0.0 23.753069 1 GTATACG 100 0.0016528184 23.753069 1 >>END_MODULE