FastQCFastQC Report
Thu 26 May 2016
SRR938024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954784
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140630.7194145235483818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109920.5623127670371765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72140.36904333164175684No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34220.17505770458526365No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33710.17244872067706712No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30970.15843182673891334No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30250.1547485553391065No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26970.13796920785109762No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.1352067543012425No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24440.12502660140455416No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24060.12308265261021167No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22840.11684155384942786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.11561379671615891No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21230.10860534974708203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.055.3419041
GTATCAA97700.041.6568571
TATCAAC125300.032.2934462
ATCAACG125950.032.0521053
TCAACGC126400.031.8627594
CAACGCA128700.031.2563925
AACGCAG132200.030.4969026
AGGTAAG12700.027.7053642
ACGCAGA149100.026.9119427
CGCAGAG149250.026.69248
GTGGTAT24900.026.572381
TAAGGTG15100.025.5067485
TGGTATC25050.025.4350722
AAGGTAA13500.025.0345341
GTACATG94300.024.9866751
TACATGG92750.024.7610512
TAAGGTA6450.024.3277134
ACCGAGT8650.024.1821278
GCAGAGT161450.024.1159179
GAGTACT97650.024.00056512-13
Produced by FastQC (version 0.11.5)