FastQCFastQC Report
Thu 26 May 2016
SRR938024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954784
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136480.6981845564522731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112260.5742833990865487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75710.38730621899913237No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33740.17260219031872576No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33590.17183484211043268No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32550.16651456119960056No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31580.16155237611930526No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29370.15024677918378707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.13837846022885392No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25550.13070497814592302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.11837625026601403No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23030.11781352824659912No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22610.11566495326337846No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21410.10952616759703374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60250.053.2961431
GTATCAA103650.040.970791
TAAGGTG12900.032.026795
TATCAAC133800.031.7361452
ATCAACG135150.031.4502583
TCAACGC137600.030.8533954
AGGTAAG12000.030.4777072
CAACGCA139200.030.430535
AACGCAG143550.029.5414756
AAGGTAA12450.027.4706731
GTAAGGT14050.026.701444
GTGGTAT27600.026.332281
ACGCAGA160500.026.2737357
CGCAGAG161300.025.9962238
TGGTATC28300.025.5111452
GGTAAGG15250.025.225073
ACGACGG950.001228670124.9936876
GAGTACT104950.023.98278212-13
GTACATG95350.023.5638661
GCAGAGT175950.023.4808549
Produced by FastQC (version 0.11.5)