##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938024_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1954784 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5481823055642 34.0 31.0 34.0 31.0 34.0 2 32.7587134946879 34.0 31.0 34.0 31.0 34.0 3 32.80229887291895 34.0 31.0 34.0 31.0 34.0 4 36.259530464747 37.0 35.0 37.0 35.0 37.0 5 36.2354853528574 37.0 37.0 37.0 35.0 37.0 6 36.273321758311916 37.0 37.0 37.0 35.0 37.0 7 36.232038936271216 37.0 37.0 37.0 35.0 37.0 8 36.219398665018744 37.0 37.0 37.0 35.0 37.0 9 37.90918536267946 39.0 38.0 39.0 35.0 39.0 10-11 37.89424330258484 39.0 38.0 39.0 35.0 39.0 12-13 37.81331441223173 39.0 38.0 39.0 35.0 39.0 14-15 39.08932521444825 40.0 38.5 41.0 36.0 41.0 16-17 38.981498467349844 40.0 38.0 41.0 36.0 41.0 18-19 39.23801299785552 40.5 39.0 41.0 36.0 41.0 20-21 39.2167850770213 41.0 39.0 41.0 36.0 41.0 22-23 39.12441221127244 40.0 39.0 41.0 35.5 41.0 24-25 39.016779859053486 40.0 38.5 41.0 35.0 41.0 26-27 38.83617397113952 40.0 38.0 41.0 35.0 41.0 28-29 38.714740861394404 40.0 38.0 41.0 35.0 41.0 30-31 38.446265930148805 40.0 38.0 41.0 34.0 41.0 32-33 38.1981075658487 40.0 38.0 41.0 34.0 41.0 34-35 38.021860215757854 40.0 37.0 41.0 33.0 41.0 36-37 38.00169865315043 40.0 37.0 41.0 33.0 41.0 38-39 37.840462168710204 40.0 37.0 41.0 33.0 41.0 40-41 37.64894177566421 40.0 37.0 41.0 33.0 41.0 42-43 37.38252743014062 40.0 36.5 41.0 32.5 41.0 44-45 37.07666780575245 40.0 36.0 41.0 31.5 41.0 46-47 36.62147429076563 39.5 35.5 41.0 30.5 41.0 48-49 36.155032218393444 39.0 35.0 41.0 30.0 41.0 50-51 35.73421385687626 39.0 35.0 40.5 28.5 41.0 52-53 35.19505249684876 38.0 35.0 40.0 27.0 41.0 54-55 34.863173629413794 38.0 34.0 40.0 26.0 41.0 56-57 34.49163922970517 38.0 34.0 40.0 26.0 41.0 58-59 34.01884376995105 37.0 33.5 40.0 24.5 41.0 60-61 33.52306265040025 36.5 33.0 39.5 23.0 41.0 62-63 33.04014075212402 36.0 32.5 39.0 22.0 40.5 64-65 32.531909407893664 35.0 32.0 39.0 20.5 40.0 66-67 31.980753628022327 35.0 31.0 38.0 18.5 40.0 68-69 31.39617343911143 35.0 31.0 37.5 15.0 39.5 70-71 30.786906123643327 34.5 30.0 37.0 9.5 39.0 72-73 30.2355930373893 34.0 29.5 36.0 7.0 39.0 74-75 29.66299345605448 34.0 29.0 35.5 4.5 38.5 76-77 28.343669172655392 32.5 27.0 34.5 2.0 36.0 78-79 29.030126346440323 34.0 29.0 35.0 2.0 37.0 80-81 28.740115531946238 34.0 29.0 35.0 2.0 36.5 82-83 28.215896487796094 33.5 27.5 35.0 2.0 36.0 84-85 27.662706467824577 33.0 26.5 35.0 2.0 35.5 86-87 27.067692389542785 33.0 25.5 35.0 2.0 35.0 88-89 26.33692597238365 32.0 24.5 34.5 2.0 35.0 90-91 25.822346867991552 32.0 23.5 34.0 2.0 35.0 92-93 25.027018586196736 31.5 19.5 34.0 2.0 35.0 94-95 24.225721102689608 31.0 12.0 34.0 2.0 35.0 96-97 23.202653336634633 31.0 2.0 34.0 2.0 35.0 98-99 21.766195651284235 30.0 2.0 34.0 2.0 35.0 100-101 17.38861659395616 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17253215041559855 1101 2 0.14129255348463232 1101 3 0.11805816586623763 1101 4 0.11634871448791273 1101 5 0.14104049068089353 1101 6 0.1532199579540432 1101 7 0.13841447457069478 1101 8 0.14436551338651782 1101 9 -0.02615034710972708 1101 10-11 0.09386915101715232 1101 12-13 0.2335460458133909 1101 14-15 0.21915332811952482 1101 16-17 0.22543157974929073 1101 18-19 0.21598192446023035 1101 20-21 0.14772228915936836 1101 22-23 0.1496577231890015 1101 24-25 0.20637253355678808 1101 26-27 0.34489778088243384 1101 28-29 0.22680082797970158 1101 30-31 0.23966101594911748 1101 32-33 0.28906705483595374 1101 34-35 0.29420605811626643 1101 36-37 0.2934383562468099 1101 38-39 0.4031903376662669 1101 40-41 0.4239317868514263 1101 42-43 0.5441554769045709 1101 44-45 0.4128193103680715 1101 46-47 0.4706403146694669 1101 48-49 0.5283863420111601 1101 50-51 0.4733421055854592 1101 52-53 0.35004749119212164 1101 54-55 0.2768823279755779 1101 56-57 0.4481112748823577 1101 58-59 0.5737163994048657 1101 60-61 0.432302777579352 1101 62-63 0.38739361355541746 1101 64-65 0.3405058406397572 1101 66-67 0.15154634908363462 1101 68-69 0.46102719946207316 1101 70-71 0.41774030591721356 1101 72-73 0.2648532510993036 1101 74-75 0.38255585769600486 1101 76-77 0.2786620580399237 1101 78-79 0.10171398333103454 1101 80-81 0.14546689066993324 1101 82-83 0.1354401601477484 1101 84-85 0.010011135873615018 1101 86-87 -0.09676588029267919 1101 88-89 -0.13022005938819703 1101 90-91 -0.16685017440832794 1101 92-93 -0.29599779370839485 1101 94-95 -0.34722937589182834 1101 96-97 -0.37595830011972 1101 98-99 -0.49308139104373083 1101 100-101 -0.7452452050630178 1102 1 0.14676637605980858 1102 2 0.1283655672387738 1102 3 0.13528540717157256 1102 4 0.014899404578734732 1102 5 0.09051010999866804 1102 6 0.05266812367118945 1102 7 0.06793343716991984 1102 8 0.04681956692517275 1102 9 0.1259819024194826 1102 10-11 0.1403287109799649 1102 12-13 0.16477593306279914 1102 14-15 0.23931613711096844 1102 16-17 0.16854437801394084 1102 18-19 0.20693506026152164 1102 20-21 0.12677225221150223 1102 22-23 0.19580203440923327 1102 24-25 0.23479736252392058 1102 26-27 0.18909875344566274 1102 28-29 0.06902866521839002 1102 30-31 0.14237310862867503 1102 32-33 -0.058357917155177574 1102 34-35 -0.016847909607982103 1102 36-37 0.08877934931014408 1102 38-39 0.17173518761024553 1102 40-41 0.19319175681637546 1102 42-43 0.11568714480283404 1102 44-45 -0.02386038737453333 1102 46-47 0.020314935059928985 1102 48-49 0.11912672960621506 1102 50-51 0.054983782780283263 1102 52-53 0.12568335207744497 1102 54-55 0.2606264580320783 1102 56-57 0.2473121282589119 1102 58-59 0.2632827982988104 1102 60-61 0.21554096022100566 1102 62-63 0.26604489719586155 1102 64-65 0.08410849611876614 1102 66-67 0.11244299040327022 1102 68-69 0.2604545075119411 1102 70-71 0.10576502523260345 1102 72-73 0.22855793036797323 1102 74-75 0.2289975396582946 1102 76-77 0.08065783883180089 1102 78-79 0.05999864877405159 1102 80-81 0.06455225216310367 1102 82-83 0.04415151386994509 1102 84-85 0.07454237469986325 1102 86-87 -0.25548855674854565 1102 88-89 -0.3622276396604214 1102 90-91 -0.6385421483779581 1102 92-93 -0.5198577732082867 1102 94-95 -0.4433069425767684 1102 96-97 -0.41696268618918 1102 98-99 -0.5449621566989009 1102 100-101 -1.180342699759212 1103 1 0.09608340272536253 1103 2 0.0674422550141145 1103 3 0.14401560287658555 1103 4 0.03691238340150704 1103 5 0.015301797734238676 1103 6 0.04235989083415603 1103 7 -0.03196412535216808 1103 8 0.005072459661825235 1103 9 0.09086851668755713 1103 10-11 0.07429645027630016 1103 12-13 0.07306115354484177 1103 14-15 0.13874560069765351 1103 16-17 0.16072492705154673 1103 18-19 0.15051930954764003 1103 20-21 0.17608058617926758 1103 22-23 0.15745019263678728 1103 24-25 0.1834759304218494 1103 26-27 0.2797979645123192 1103 28-29 0.1982314141562398 1103 30-31 0.22359671095081524 1103 32-33 0.2725336013852697 1103 34-35 0.24471607717002541 1103 36-37 0.2342926957260829 1103 38-39 0.2403434412415848 1103 40-41 0.32211952106587205 1103 42-43 0.25956038367930745 1103 44-45 0.27197058340096447 1103 46-47 0.22868117375800523 1103 48-49 0.19164698131947944 1103 50-51 0.22309558678210806 1103 52-53 0.219400008763607 1103 54-55 0.17879763372797441 1103 56-57 0.16971829541551386 1103 58-59 0.12450387803414742 1103 60-61 0.08968049676954593 1103 62-63 0.03855770614173082 1103 64-65 0.008407739683612192 1103 66-67 -0.07127945005175107 1103 68-69 -0.022574335200229 1103 70-71 -0.19668773592046662 1103 72-73 -0.41172573565516757 1103 74-75 -0.39987554013612936 1103 76-77 -0.2532516934783331 1103 78-79 -0.3082116724694295 1103 80-81 -0.29709437205895384 1103 82-83 -0.5215121594027927 1103 84-85 -0.7359595121268931 1103 86-87 -0.725725867398296 1103 88-89 -0.8788265733561573 1103 90-91 -1.0310722802228902 1103 92-93 -0.9655674723273151 1103 94-95 -0.8813848223311922 1103 96-97 -0.9243033285852285 1103 98-99 -1.0621940096979898 1103 100-101 -1.2902286265655611 1104 1 0.23548854885586934 1104 2 0.16842241287255888 1104 3 0.14764972126424425 1104 4 0.12798367450997006 1104 5 0.07557119295353232 1104 6 0.1415810592183675 1104 7 0.13089153156388278 1104 8 0.14888124854741847 1104 9 0.15154077896849572 1104 10-11 0.17781692367459812 1104 12-13 0.2341752553135663 1104 14-15 0.2660890089261656 1104 16-17 0.23084632629306867 1104 18-19 0.20882922053333886 1104 20-21 0.2784925046070015 1104 22-23 0.2419299670741495 1104 24-25 0.2952368054855796 1104 26-27 0.29519810779566313 1104 28-29 0.13971390057020017 1104 30-31 0.10466456079137032 1104 32-33 0.17086921325101656 1104 34-35 0.19779225686375668 1104 36-37 0.21025370935247878 1104 38-39 0.3200204609480011 1104 40-41 0.2283362533526656 1104 42-43 0.23861666088188116 1104 44-45 0.16713810984816035 1104 46-47 0.09926294651791068 1104 48-49 0.17385189796059564 1104 50-51 0.3600715947343076 1104 52-53 0.3702353678316115 1104 54-55 0.18905196837102523 1104 56-57 0.3768343439280031 1104 58-59 0.47206804775651534 1104 60-61 0.18595593445832037 1104 62-63 0.20912054254041834 1104 64-65 0.20762349001546454 1104 66-67 0.03352968832503578 1104 68-69 0.14390068798072164 1104 70-71 0.13558760199031283 1104 72-73 -0.04614265263853312 1104 74-75 -0.1387998475306702 1104 76-77 -0.052653669243547085 1104 78-79 -0.07378032522779066 1104 80-81 -0.14975151100007977 1104 82-83 0.005517752805982212 1104 84-85 -0.19517713463723751 1104 86-87 -0.17179049725277906 1104 88-89 -0.27807641450261755 1104 90-91 -0.20965611398845851 1104 92-93 -0.35215301841200386 1104 94-95 -0.5130472497742176 1104 96-97 -0.6317166994265087 1104 98-99 -0.6072617447402138 1104 100-101 -1.0559004100730647 1105 1 0.17752439743238568 1105 2 0.1899914006497383 1105 3 0.14771725932901347 1105 4 0.13965597981972167 1105 5 0.08888875693406817 1105 6 0.11383705047297354 1105 7 0.08431775329606239 1105 8 0.12581228747133366 1105 9 0.18823986390624725 1105 10-11 0.15136988137063412 1105 12-13 0.17248512309602404 1105 14-15 0.1709365142585355 1105 16-17 0.06791624232598537 1105 18-19 0.14677587488608168 1105 20-21 0.12893535012702984 1105 22-23 0.12938367208672474 1105 24-25 0.14520374324689556 1105 26-27 0.228761463212372 1105 28-29 0.14426133271015118 1105 30-31 0.19862807138856198 1105 32-33 0.1932800708587834 1105 34-35 0.280245632039275 1105 36-37 0.3300521003758874 1105 38-39 0.31309898729884367 1105 40-41 0.2717098268073812 1105 42-43 0.44009527330449316 1105 44-45 0.5427553763470812 1105 46-47 0.4920615825631316 1105 48-49 0.49618280934750914 1105 50-51 0.4749275344715045 1105 52-53 0.40511445898915355 1105 54-55 0.4821330077661372 1105 56-57 0.4907745931591663 1105 58-59 0.5508001540959881 1105 60-61 0.2538689925504798 1105 62-63 0.42342981661616363 1105 64-65 0.49838860205012026 1105 66-67 0.49027980376800784 1105 68-69 0.30329837409520977 1105 70-71 0.22537474714060934 1105 72-73 0.1595727953587236 1105 74-75 0.12211854449795467 1105 76-77 0.14547591902556078 1105 78-79 0.07731174957297071 1105 80-81 -0.16528840720193116 1105 82-83 -0.09636223990618831 1105 84-85 -0.08449716768376803 1105 86-87 -0.15149295281116437 1105 88-89 -0.15143233382132948 1105 90-91 -0.4688440394390021 1105 92-93 -0.6492804517456534 1105 94-95 -0.5275019662871188 1105 96-97 -0.6909558281540562 1105 98-99 -0.9544580511933951 1105 100-101 -1.1981143706622461 1106 1 0.151368661911242 1106 2 0.16180101587293194 1106 3 0.16907894758303854 1106 4 0.10120597501441608 1106 5 0.07034212301643805 1106 6 0.13319328623195048 1106 7 0.08424080766651088 1106 8 0.1386543001500229 1106 9 0.08707765035384085 1106 10-11 0.08673479221711489 1106 12-13 0.03699588271057763 1106 14-15 0.12176460738159989 1106 16-17 0.09799255535904905 1106 18-19 0.1227426830353906 1106 20-21 0.25027358973268576 1106 22-23 0.2558562837004743 1106 24-25 0.2830199046271247 1106 26-27 0.2323230118085533 1106 28-29 0.2867733064233349 1106 30-31 0.2235234800763024 1106 32-33 0.38209400111988856 1106 34-35 0.4621519432840415 1106 36-37 0.2986777480269538 1106 38-39 0.24317046461843717 1106 40-41 0.26271479147321486 1106 42-43 0.36204138338357694 1106 44-45 0.2934705449157988 1106 46-47 0.23173490122509577 1106 48-49 0.21879351069019748 1106 50-51 0.0534018881586249 1106 52-53 0.10436334190892893 1106 54-55 -0.1493362036415249 1106 56-57 -0.124228253524997 1106 58-59 0.04553622146264047 1106 60-61 -0.0948134732132786 1106 62-63 -0.08621788797101004 1106 64-65 0.09175117472268113 1106 66-67 -0.13168494965926314 1106 68-69 -0.24945044009052353 1106 70-71 -0.8115935220632515 1106 72-73 -0.7187980618946632 1106 74-75 -0.6688283916061515 1106 76-77 -0.4349223795332122 1106 78-79 -0.2985113861834492 1106 80-81 -0.45410884590758727 1106 82-83 -0.39069159031476275 1106 84-85 -0.6818485957094929 1106 86-87 -0.6954754396209211 1106 88-89 -0.8900541129016091 1106 90-91 -1.144958443295895 1106 92-93 -1.0099355662273695 1106 94-95 -0.9621109547531823 1106 96-97 -0.8603282133712504 1106 98-99 -1.1210342383154348 1106 100-101 -1.5751592083708363 1107 1 0.002239766079860317 1107 2 -0.03579831041113124 1107 3 0.0173207317640518 1107 4 -0.007196354957251572 1107 5 -0.00952035281114405 1107 6 0.042433948487541784 1107 7 -0.0028839065957342314 1107 8 -0.030786996685876034 1107 9 0.023736241325401863 1107 10-11 0.02231266791495301 1107 12-13 0.01817826091214414 1107 14-15 0.058974769637472946 1107 16-17 0.026350524141378173 1107 18-19 0.007823541843791304 1107 20-21 0.016049649828858037 1107 22-23 -0.027038530604336586 1107 24-25 0.08065477817362421 1107 26-27 -0.025421273296181823 1107 28-29 0.054765323427709234 1107 30-31 -0.10677843056101466 1107 32-33 -0.0751686858606746 1107 34-35 0.026902767627298374 1107 36-37 -0.051993332705144724 1107 38-39 -0.0757523972613896 1107 40-41 -0.11752583658075366 1107 42-43 0.029392035821622642 1107 44-45 -0.021905860784997344 1107 46-47 0.003769438137894099 1107 48-49 -0.06648812847593888 1107 50-51 -0.04909603591488576 1107 52-53 0.03796579343653406 1107 54-55 0.14656700239635256 1107 56-57 0.15283882819614547 1107 58-59 0.2543489148630229 1107 60-61 0.10853088500444841 1107 62-63 0.17745715510341142 1107 64-65 0.13622520139210081 1107 66-67 0.04203505453464018 1107 68-69 0.16143708646479737 1107 70-71 -0.07602128122326235 1107 72-73 -0.15498315660232365 1107 74-75 -0.17937048175154047 1107 76-77 -0.36485768800102747 1107 78-79 -0.09285028840100651 1107 80-81 0.123820817610099 1107 82-83 0.11117940676352234 1107 84-85 -0.16763824113956716 1107 86-87 -0.05144216536481849 1107 88-89 -0.2811436645295231 1107 90-91 -0.45379645150540426 1107 92-93 -0.4687305981329999 1107 94-95 -0.4506389728923743 1107 96-97 -0.503507988885243 1107 98-99 -0.4558479600072829 1107 100-101 -1.1988969020936047 1108 1 -0.0018934240099710564 1108 2 0.03186864406072942 1108 3 0.021055168863242102 1108 4 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46-47 -0.9236794288028136 2316 48-49 -0.8058808223407397 2316 50-51 -0.5735991688981841 2316 52-53 -0.6161128486970284 2316 54-55 -0.5174989549650277 2316 56-57 -0.3677160723009294 2316 58-59 -0.44982842498741604 2316 60-61 -0.3805845183587877 2316 62-63 -0.1940774691868512 2316 64-65 0.04292790323130902 2316 66-67 -0.1091269103184409 2316 68-69 -0.11593577114496512 2316 70-71 0.12046175990985475 2316 72-73 0.3303676153424817 2316 74-75 0.422578409582961 2316 76-77 0.43635826700731783 2316 78-79 0.3924439244847022 2316 80-81 0.48633966883084767 2316 82-83 0.4262276136470611 2316 84-85 0.5078620366906179 2316 86-87 0.6610021876448791 2316 88-89 0.9822135560115193 2316 90-91 0.9838140491372513 2316 92-93 1.0579569666022977 2316 94-95 1.3844351345862442 2316 96-97 1.6440507105672566 2316 98-99 2.13198480478518 2316 100-101 2.626766166524625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 12.0 5 31.0 6 66.0 7 206.0 8 492.0 9 920.0 10 1658.0 11 2680.0 12 4202.0 13 6405.0 14 8200.0 15 11778.0 16 16102.0 17 23056.0 18 23688.0 19 17719.0 20 16010.0 21 16267.0 22 17369.0 23 18591.0 24 21018.0 25 23846.0 26 27442.0 27 32232.0 28 38897.0 29 47104.0 30 57252.0 31 69733.0 32 87360.0 33 113798.0 34 156729.0 35 229883.0 36 319273.0 37 366889.0 38 170659.0 39 7212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42162649992172 18.011544038121315 14.68636581769588 22.88046364426108 2 16.333109916141808 19.719517623523274 38.8132453990555 25.134127061279415 3 19.66910927692222 23.45898374247865 29.50292207675932 27.36898490383981 4 13.448653891506115 16.21658260789049 34.89988402804813 35.43487947255527 5 14.00318094827864 37.04259800292925 33.12156000497247 15.832661043819634 6 33.54437438163574 35.02174698197318 16.024778565487015 15.409100070904064 7 29.667472211763553 33.09695598081424 18.864795291960647 18.370776515461554 8 28.034606381063078 33.43417994008545 18.578267470984006 19.952946207867466 9 27.304653608787465 16.105615761127574 19.14881644212353 37.44091418796143 10-11 25.929258680242935 26.014434331363468 25.50220382405422 22.554103164339384 12-13 27.073698771414307 23.301307367421607 26.49639241076179 23.128601450402297 14-15 23.475918566961873 24.377296928970157 25.256345458117114 26.89043904595086 16-17 22.676010151780478 27.817766229726747 26.18810465480928 23.318118963683492 18-19 22.80766059063303 25.607074745854273 29.174936975133825 22.41032768837887 20-21 23.01988352677329 24.539412027108877 29.979475993255523 22.46122845286231 22-23 23.136801815443548 24.478638049012062 29.912563229492363 22.471996906052023 24-25 22.925368888961646 24.83784130829924 29.742812401360602 22.49397740137851 26-27 22.00285713847144 25.325099275840245 29.748726337403287 22.923317248285027 28-29 22.470683994926965 25.717660817875743 29.478745288796095 22.332909898401194 30-31 22.328082794466727 25.185934558402227 29.311870753867563 23.174111893263486 32-33 22.037601085337304 25.206979390050254 29.39066413475862 23.364755389853816 34-35 23.0797526866042 25.364664471849814 29.195029174692582 22.360553666853406 36-37 23.16775025591259 24.76126335132182 29.10065076743083 22.97033562533476 38-39 22.504138666295603 25.191355763642765 29.033767219716626 23.270738350345006 40-41 23.00421640458658 25.020156071428207 29.14964399466734 22.825983529317874 42-43 22.629860845018257 25.089590515708842 28.883393947083146 23.397154692189755 44-45 22.5504710494868 25.354796233241117 29.25522717599489 22.839505541277195 46-47 23.267652539349008 25.80292180843649 28.160294048059054 22.769131604155447 48-49 22.86367777741968 25.883030919702698 27.939663582421083 23.313627720456545 50-51 23.144194945765264 26.173870893066574 27.86254650655664 22.81938765461152 52-53 23.59113424488213 25.726252953256434 27.826914871519914 22.855697930341517 54-55 22.886207696591377 26.194456332084936 27.860462605860043 23.058873365463644 56-57 22.884865112119186 26.376252406062513 27.67317254371845 23.06570993809985 58-59 22.912177079098527 25.79028041122853 27.586507737718623 23.711034771954324 60-61 22.435695097638476 26.254622806133487 27.99475216540962 23.31492993081842 62-63 22.64729239069478 26.260474102819153 28.178969516113845 22.91326399037222 64-65 22.212520131553866 26.389751999723693 27.929561627819215 23.468166240903223 66-67 22.173986340860434 26.396567333438902 27.895864729647833 23.533581596052834 68-69 22.875028657048816 25.88468403556749 28.093784286111976 23.14650302127172 70-71 22.669577975361324 26.22224439387602 27.479402746812077 23.62877488395058 72-73 22.584161941265187 26.431203202154414 27.272490080238033 23.712144776342363 74-75 22.448995254676444 26.956477025128034 27.212380154933573 23.38214756526195 76-77 22.70889537866025 26.345717198618985 27.565248425633488 23.380138997087275 78-79 22.733573699087728 26.569353673338643 27.584196241615683 23.112876385957946 80-81 22.920492167710307 26.58665453762195 27.20864327459649 23.28421002007125 82-83 22.73009899328172 26.73297094477583 27.27187125141755 23.265058810524895 84-85 22.583563398273434 26.90553509254661 27.199470716321294 23.311430792858662 86-87 23.019327614555095 26.898641709144705 26.772474128097834 23.309556548202362 88-89 22.791039792867807 27.05815231226406 26.512767310159735 23.638040584708396 90-91 22.60806980206595 26.901822039221447 26.743522899133488 23.74658525957912 92-93 22.787572977886665 26.452208454957816 26.992332444767836 23.76788612238768 94-95 22.59258150679973 26.2080934644902 27.18657670801582 24.01274832069425 96-97 22.88913590448427 25.83324912347127 27.087775670044017 24.189839302000443 98-99 22.46919328225528 26.320845218147632 26.749568309435794 24.46039319016129 100-101 22.872718295969516 25.56275957161331 26.86528944753056 24.699232684886617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1937.0 1 1302.5 2 923.0 3 1222.0 4 970.5 5 861.5 6 1754.0 7 3917.0 8 6215.0 9 6712.0 10 5090.5 11 3420.5 12 3084.5 13 3186.0 14 3308.0 15 3359.0 16 3454.0 17 3634.0 18 3788.0 19 3969.0 20 4206.0 21 4744.5 22 5488.0 23 6440.0 24 7562.5 25 9086.0 26 11001.0 27 12944.0 28 14740.0 29 16633.5 30 18516.0 31 21928.0 32 28379.0 33 29707.0 34 27069.5 35 27732.5 36 30326.0 37 33249.5 38 36294.5 39 39051.0 40 41651.0 41 45858.0 42 48537.5 43 51193.5 44 55089.5 45 60260.5 46 64137.5 47 68307.5 48 70969.5 49 69977.0 50 70855.5 51 71340.0 52 72562.0 53 76768.5 54 81066.5 55 78104.0 56 72595.5 57 68457.5 58 64780.5 59 58776.5 60 47551.0 61 39069.5 62 33423.0 63 25157.0 64 17794.5 65 13757.0 66 10394.0 67 8011.5 68 6237.5 69 4758.0 70 3587.0 71 2380.5 72 1557.0 73 1157.5 74 835.5 75 583.0 76 404.5 77 237.5 78 145.0 79 89.0 80 50.0 81 31.0 82 18.0 83 10.5 84 6.0 85 4.0 86 4.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009310491593956161 2 0.015040024882544568 3 0.009259335046736622 4 5.1156547219539345E-5 5 0.0010742874916103262 6 0.0015346964165861804 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.185047555126295E-4 14-15 0.0 16-17 0.0027112970026355854 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003222862474830979 26-27 0.01949064449064449 28-29 0.006906133874637811 30-31 0.003274019022050518 32-33 0.0 34-35 0.009157021952297542 36-37 7.673482082930902E-4 38-39 0.0024555142665378883 40-41 0.001943948794342495 42-43 0.0029159231915137428 44-45 0.0 46-47 7.673482082930901E-5 48-49 5.627220194149328E-4 50-51 0.004425041334490153 52-53 0.008287360649565374 54-55 0.007136338337125739 56-57 0.01770016533796061 58-59 0.025245756052842665 60-61 0.0037344279470263725 62-63 0.022508880776597313 64-65 0.023097181069622014 66-67 0.013735532928446315 68-69 0.032740190220505186 70-71 0.01721417813937499 72-73 0.005115654721953935 74-75 0.017060708497716372 76-77 0.021818267389133533 78-79 0.027624535498551244 80-81 0.012635667163226217 82-83 0.036423461620312014 84-85 0.02273908523908524 86-87 0.03258672057884656 88-89 0.0243760947501105 90-91 0.029287123283186278 92-93 0.02611541735557484 94-95 0.016907238856057754 96-97 0.017469960875472687 98-99 0.01324954572986069 100-101 0.012354306153518752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1954784.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.390663393210275 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99703113637571 31.959959744853332 2 14.339573393025937 12.730863513840967 3 5.281494179241145 7.033470909716789 4 2.559814010071687 4.545273682812641 5 1.471067192228442 3.265082427950386 6 0.8975492711037907 2.390568454343376 7 0.6098771609778222 1.8950996234921254 8 0.4568975619178571 1.6225588701019236 9 0.32570732099319283 1.30125276458218 >10 1.8294235112826507 15.036420452185318 >50 0.14431455735189236 4.414734497711286 >100 0.07633106700545346 6.483506306608108 >500 0.005402347431013436 1.6775229475762554 >1k 0.00517246030628946 3.988205315308661 >5k 1.14943562361988E-4 0.3863042928521407 >10k+ 2.29887124723976E-4 1.269176196064476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13648 0.6981845564522731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11226 0.5742833990865487 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7571 0.38730621899913237 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3374 0.17260219031872576 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3359 0.17183484211043268 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3255 0.16651456119960056 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3158 0.16155237611930526 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2937 0.15024677918378707 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2705 0.13837846022885392 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2555 0.13070497814592302 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2314 0.11837625026601403 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2303 0.11781352824659912 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2261 0.11566495326337846 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2141 0.10952616759703374 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1156547219539345E-5 2 1.0231309443907869E-4 0.0 0.0 0.0 5.1156547219539345E-5 3 1.0231309443907869E-4 0.0 0.0 0.0 5.1156547219539345E-5 4 1.0231309443907869E-4 0.0 0.0 0.0 1.0231309443907869E-4 5 1.5346964165861805E-4 0.0 0.0 0.0 1.0231309443907869E-4 6 1.5346964165861805E-4 0.0 0.0 0.0 1.0231309443907869E-4 7 1.5346964165861805E-4 0.0 0.0 0.0 1.0231309443907869E-4 8 1.5346964165861805E-4 0.0 0.0 0.0 1.0231309443907869E-4 9 1.5346964165861805E-4 0.0 0.0 0.0 1.0231309443907869E-4 10-11 1.5346964165861805E-4 0.0 0.0 2.5578273609769672E-5 1.0231309443907869E-4 12-13 1.5346964165861805E-4 0.0 0.0 5.1156547219539345E-5 1.5346964165861805E-4 14-15 1.5346964165861805E-4 0.0 0.0 1.0231309443907869E-4 1.5346964165861805E-4 16-17 1.5346964165861805E-4 0.0 0.0 1.5346964165861805E-4 2.557827360976967E-4 18-19 2.3020446248792707E-4 0.0 0.0 1.5346964165861805E-4 2.557827360976967E-4 20-21 3.069392833172361E-4 0.0 0.0 2.0462618887815738E-4 2.557827360976967E-4 22-23 3.5809583053677543E-4 0.0 0.0 3.069392833172361E-4 4.0925237775631476E-4 24-25 4.0925237775631476E-4 0.0 0.0 8.185047555126295E-4 4.604089249758541E-4 26-27 4.0925237775631476E-4 0.0 0.0 0.001713744331854568 4.604089249758541E-4 28-29 5.115654721953934E-4 0.0 0.0 0.006675929412149884 4.604089249758541E-4 30-31 5.627220194149328E-4 0.0 0.0 0.01805826116849739 4.604089249758541E-4 32-33 5.627220194149328E-4 0.0 0.0 0.04184605562558319 5.627220194149328E-4 34-35 5.627220194149328E-4 0.0 0.0 0.0768371339237481 5.627220194149328E-4 36-37 5.627220194149328E-4 0.0 0.0 0.12942606446543453 5.627220194149328E-4 38-39 6.650351138540115E-4 0.0 0.0 0.21125096174308772 5.627220194149328E-4 40-41 6.650351138540115E-4 0.0 0.0 0.300058727716208 5.627220194149328E-4 42-43 7.673482082930902E-4 0.0 0.0 0.38781778447132775 5.883002930247025E-4 44-45 9.208178499517082E-4 0.0 0.0 0.4938141503102133 6.138785666344722E-4 46-47 0.0010231309443907868 0.0 0.0 0.6144157103802773 6.650351138540115E-4 48-49 0.0011254440388298655 0.0 0.0 0.7416676215888814 6.650351138540115E-4 50-51 0.0011254440388298655 0.0 0.0 0.8803274428274428 6.650351138540115E-4 52-53 0.0011254440388298655 0.0 0.0 1.0093954114623407 7.417699346833205E-4 54-55 0.0011254440388298655 0.0 0.0 1.1465205362843158 8.952395763419385E-4 56-57 0.0012277571332689444 0.0 0.0 1.3023689573886426 9.208178499517082E-4 58-59 0.0012277571332689444 0.0 0.0 1.4692160361451698 9.208178499517082E-4 60-61 0.0012277571332689444 0.0 0.0 1.648212794866338 0.0010231309443907868 62-63 0.0012277571332689444 0.0 0.0 1.816927087596379 0.0010742874916103262 64-65 0.0012789136804884837 0.0 0.0 1.996051737685596 0.0010742874916103262 66-67 0.0012789136804884837 0.0 0.0 2.197582955456971 0.0010742874916103262 68-69 0.0012789136804884837 0.0 0.0 2.426150408433873 0.0010742874916103262 70-71 0.0012789136804884837 0.0 0.0 2.6566106536578977 0.0011254440388298655 72-73 0.0012789136804884837 0.0 0.0 2.8937212500204623 0.0013812267749275624 74-75 0.0013044919540982534 0.0 0.0 3.125562722019415 0.0013812267749275624 76-77 0.001330070227708023 0.0 0.0 3.370346800464911 0.0013812267749275624 78-79 0.001330070227708023 0.0 0.0 3.639506973660517 0.0013812267749275624 80-81 0.001637009511025259 0.0 0.0 3.926597516656572 0.001406805048537332 82-83 0.001637009511025259 0.0 0.0 4.216322621834433 0.0014323833221471017 84-85 0.0016625877846350287 0.0 0.0 4.51538379687986 0.0014323833221471017 86-87 0.0016881660582447984 0.0 0.0 4.816184294530752 0.0014323833221471017 88-89 0.0016881660582447984 0.0 0.0 5.142204969960876 0.0014323833221471017 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6025 0.0 53.296143 1 GTATCAA 10365 0.0 40.97079 1 TAAGGTG 1290 0.0 32.02679 5 TATCAAC 13380 0.0 31.736145 2 ATCAACG 13515 0.0 31.450258 3 TCAACGC 13760 0.0 30.853395 4 AGGTAAG 1200 0.0 30.477707 2 CAACGCA 13920 0.0 30.43053 5 AACGCAG 14355 0.0 29.541475 6 AAGGTAA 1245 0.0 27.470673 1 GTAAGGT 1405 0.0 26.70144 4 GTGGTAT 2760 0.0 26.33228 1 ACGCAGA 16050 0.0 26.273735 7 CGCAGAG 16130 0.0 25.996223 8 TGGTATC 2830 0.0 25.511145 2 GGTAAGG 1525 0.0 25.22507 3 ACGACGG 95 0.0012286701 24.993687 6 GAGTACT 10495 0.0 23.982782 12-13 GTACATG 9535 0.0 23.563866 1 GCAGAGT 17595 0.0 23.480854 9 >>END_MODULE