FastQCFastQC Report
Thu 26 May 2016
SRR938023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595577
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205821.2899408803210375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153660.9630371959485502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108630.6808195405173176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51840.32489814029658237No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.22794261887705827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33040.2070724258371736No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25580.1603181795676423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.16000481330578217No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23850.14947570690728182No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.12672531629623643No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.10579245000397976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95800.038.1698761
GTATCAA149800.034.626451
TATCAAC183150.028.4096342
ATCAACG183400.028.3190883
TCAACGC184950.028.0817574
CAACGCA186550.027.8154355
AACGCAG191100.027.223496
GTACATG117600.025.742891
GAGTACT112600.025.35469212-13
TACATGG118500.024.8216572
ACGCAGA212750.024.4246987
CGCAGAG214700.024.1143688
GTACTTT119000.023.85435714-15
GCAGAGT218300.023.2380089
ACATGGG119850.023.1147373
AGTACTT114700.022.17779412-13
ACTTTTT131100.021.77884916-17
AGAGTAC180350.021.2285710-11
GTATAGA3401.8189894E-1221.0330961
CATGGGA78100.020.8689964
Produced by FastQC (version 0.11.5)