##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938023_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1595577 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07484063758753 33.0 31.0 34.0 30.0 34.0 2 32.199737148379555 34.0 31.0 34.0 30.0 34.0 3 32.276060008385684 34.0 31.0 34.0 30.0 34.0 4 35.80578123149181 37.0 35.0 37.0 35.0 37.0 5 35.8352044432829 37.0 35.0 37.0 35.0 37.0 6 35.90296927067763 37.0 36.0 37.0 35.0 37.0 7 35.8429646453916 37.0 36.0 37.0 35.0 37.0 8 35.822712410620106 37.0 36.0 37.0 35.0 37.0 9 37.47151030630299 39.0 38.0 39.0 35.0 39.0 10-11 37.391288543266796 39.0 37.0 39.0 35.0 39.0 12-13 37.26619179143344 39.0 37.0 39.0 34.0 39.0 14-15 38.41780810327549 40.0 38.0 41.0 34.0 41.0 16-17 38.282274688090894 40.0 38.0 41.0 34.0 41.0 18-19 38.5082474866459 40.0 38.0 41.0 34.0 41.0 20-21 38.53105522328286 40.0 38.0 41.0 34.0 41.0 22-23 38.42487952634063 40.0 38.0 41.0 34.0 41.0 24-25 38.29850298669385 40.0 38.0 41.0 34.0 41.0 26-27 38.09734409558423 40.0 38.0 41.0 33.5 41.0 28-29 37.89693791023561 40.0 38.0 41.0 33.0 41.0 30-31 37.60280481606341 40.0 37.0 41.0 33.0 41.0 32-33 37.23896903753313 39.5 36.0 41.0 32.5 41.0 34-35 36.94029808652293 39.0 35.0 41.0 31.0 41.0 36-37 36.644703138739146 39.0 35.0 41.0 31.0 41.0 38-39 36.33501987055466 39.0 35.0 40.0 30.0 41.0 40-41 36.155117553085816 39.0 35.0 40.0 30.0 41.0 42-43 35.86574762609388 39.0 35.0 40.0 29.5 41.0 44-45 35.44784519957357 38.0 35.0 40.0 27.5 41.0 46-47 34.88612959449779 38.0 34.5 40.0 25.0 41.0 48-49 34.347553894296546 38.0 34.0 40.0 23.0 41.0 50-51 33.49668615178083 37.5 33.0 39.5 20.5 40.5 52-53 33.48717297880328 37.5 33.0 39.5 20.5 40.5 54-55 33.497428203088916 38.0 33.0 40.0 18.5 41.0 56-57 33.069834298188056 37.0 33.0 40.0 14.0 41.0 58-59 32.659856277697656 37.0 32.0 40.0 9.0 41.0 60-61 32.14447688829809 36.0 31.5 39.5 6.5 41.0 62-63 31.580884219313766 35.5 31.0 39.0 2.0 41.0 64-65 31.02727257913595 35.0 30.0 39.0 2.0 40.0 66-67 30.384905899245226 35.0 29.0 38.0 2.0 40.0 68-69 29.732388972766593 35.0 28.5 37.5 2.0 40.0 70-71 28.970920550998166 34.0 27.0 37.0 2.0 39.5 72-73 28.229892383758354 34.0 26.0 36.0 2.0 39.0 74-75 27.451490275931526 34.0 25.5 36.0 2.0 39.0 76-77 26.65460833291029 33.0 22.5 35.0 2.0 37.5 78-79 25.844552785606712 32.5 19.5 35.0 2.0 37.0 80-81 25.062058114399996 32.0 15.0 35.0 2.0 36.5 82-83 24.257736856322197 31.0 6.5 35.0 2.0 36.0 84-85 23.44896861762234 31.0 2.0 35.0 2.0 35.5 86-87 22.692271823923257 30.5 2.0 34.0 2.0 35.0 88-89 21.875251084717316 29.5 2.0 34.0 2.0 35.0 90-91 21.01865876732994 29.0 2.0 34.0 2.0 35.0 92-93 20.10858517012968 28.0 2.0 34.0 2.0 35.0 94-95 19.18192509668916 26.0 2.0 34.0 2.0 35.0 96-97 18.079113699934254 24.5 2.0 33.5 2.0 35.0 98-99 16.769348643155425 10.5 2.0 33.0 2.0 35.0 100-101 13.60613777962455 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43828518163937247 1101 2 0.4384652701576286 1101 3 0.3904490659319748 1101 4 0.14193557599556783 1101 5 0.2793849333413192 1101 6 0.1862964179147184 1101 7 0.21293679589761183 1101 8 0.2437895944047952 1101 9 0.3325618832163997 1101 10-11 0.34806451159349905 1101 12-13 0.4207243267328593 1101 14-15 0.4958490383956118 1101 16-17 0.5915053689324736 1101 18-19 0.6190814691064119 1101 20-21 0.49510539661789466 1101 22-23 0.560767782568675 1101 24-25 0.6551374758982931 1101 26-27 0.7733218712378189 1101 28-29 0.9672900281735721 1101 30-31 0.9047674182976877 1101 32-33 0.909511475912602 1101 34-35 0.9927806272294859 1101 36-37 1.0615873805761922 1101 38-39 1.0670574644539457 1101 40-41 1.2622899534749479 1101 42-43 1.2949081647327247 1101 44-45 1.5152257772836677 1101 46-47 1.7662108217365002 1101 48-49 1.814305271594428 1101 50-51 1.8132499749468423 1101 52-53 1.8927607888769273 1101 54-55 2.0916365648757065 1101 56-57 2.196726668399407 1101 58-59 2.4139501790629794 1101 60-61 2.5638794634229853 1101 62-63 2.611923657241025 1101 64-65 2.6323589553279056 1101 66-67 2.6611349236451467 1101 68-69 3.083832927434983 1101 70-71 3.314731021588944 1101 72-73 3.5912535582144045 1101 74-75 3.7402723847713126 1101 76-77 4.09177377252357 1101 78-79 4.173998167648946 1101 80-81 4.452140325508029 1101 82-83 4.601560209622551 1101 84-85 4.778246842480453 1101 86-87 5.017075993419962 1101 88-89 4.854262727958847 1101 90-91 4.817176628014327 1101 92-93 5.344983122358151 1101 94-95 5.494473075341329 1101 96-97 5.412937680047641 1101 98-99 5.248599781573876 1101 100-101 4.6099296899957825 1102 1 0.27877473124695484 1102 2 0.33862291598001804 1102 3 0.29462413755064176 1102 4 0.10284195471847113 1102 5 0.04780449159399325 1102 6 0.03314265390881843 1102 7 0.15705940366463267 1102 8 0.26789203028701536 1102 9 0.28876643290873716 1102 10-11 0.3060086744602728 1102 12-13 0.4649838151467307 1102 14-15 0.4803863987604018 1102 16-17 0.5015815644132928 1102 18-19 0.5090218010101992 1102 20-21 0.37019756857049657 1102 22-23 0.2824190801802473 1102 24-25 0.5007780603744152 1102 26-27 0.4687762048028148 1102 28-29 0.49271253592872455 1102 30-31 0.5670123032840451 1102 32-33 0.6084948298698549 1102 34-35 0.6977424447332581 1102 36-37 0.6786711871295097 1102 38-39 0.8567888732651667 1102 40-41 0.8575470178724203 1102 42-43 1.0979123347189983 1102 44-45 1.066569792675331 1102 46-47 1.1807261032105671 1102 48-49 1.2898606340950707 1102 50-51 1.3196416231084882 1102 52-53 1.4822216147376182 1102 54-55 1.4984083428832662 1102 56-57 1.813526542960794 1102 58-59 1.8032696745963506 1102 60-61 2.0105140217604145 1102 62-63 2.0787724782875934 1102 64-65 1.9720073034369996 1102 66-67 2.2763795289373014 1102 68-69 2.317762164802126 1102 70-71 2.6247911134642514 1102 72-73 2.7171678616063275 1102 74-75 2.983032118977068 1102 76-77 3.1174696177933505 1102 78-79 3.1393944858561937 1102 80-81 3.318435902949421 1102 82-83 3.7241351337660937 1102 84-85 3.772011992583007 1102 86-87 4.089220146116045 1102 88-89 4.366123245308362 1102 90-91 4.686276563599904 1102 92-93 4.802673017769472 1102 94-95 4.955795817022548 1102 96-97 5.006398980540919 1102 98-99 5.043263979021031 1102 100-101 4.19239180414492 1103 1 0.45965209946978547 1103 2 0.3785245027097801 1103 3 0.2596473080626893 1103 4 0.1809912612405995 1103 5 0.20076057405809422 1103 6 0.23040722349404064 1103 7 0.2602439162651322 1103 8 0.2771172509390638 1103 9 0.2759960420393952 1103 10-11 0.28787140350447515 1103 12-13 0.4050106700158196 1103 14-15 0.5894321093500565 1103 16-17 0.5468234219842358 1103 18-19 0.52681929237243 1103 20-21 0.44971315749450014 1103 22-23 0.5617854526441874 1103 24-25 0.6139758366602521 1103 26-27 0.645008212859274 1103 28-29 0.8311309642638918 1103 30-31 0.7101587851324425 1103 32-33 0.8237063119638748 1103 34-35 0.8806001548644176 1103 36-37 0.8810672473237204 1103 38-39 1.0614426255781169 1103 40-41 1.2272374500065695 1103 42-43 1.0399921952046824 1103 44-45 1.1083166282220063 1103 46-47 1.2535719824619136 1103 48-49 1.3818723339755294 1103 50-51 1.6083140673928185 1103 52-53 1.4996680687486545 1103 54-55 1.5245212233954817 1103 56-57 1.674957874733046 1103 58-59 1.7350292416434598 1103 60-61 1.9615008153872893 1103 62-63 2.2111340283375363 1103 64-65 2.234820678471795 1103 66-67 2.516444046122775 1103 68-69 2.636183745309424 1103 70-71 2.836328472844624 1103 72-73 3.1347641461369413 1103 74-75 3.167503782177711 1103 76-77 3.526654310517973 1103 78-79 3.684239251612958 1103 80-81 3.9817347943673234 1103 82-83 4.055699634576651 1103 84-85 4.188155002948335 1103 86-87 4.343516515796242 1103 88-89 4.419344806165753 1103 90-91 4.901951660621307 1103 92-93 4.739574309750672 1103 94-95 4.910499717528534 1103 96-97 5.06771179882843 1103 98-99 4.698498222254095 1103 100-101 4.310996374385139 1104 1 0.4834953478172821 1104 2 0.3587076944000529 1104 3 0.3413640219437042 1104 4 0.29732365028687013 1104 5 0.3460183644263637 1104 6 0.4047226153731742 1104 7 0.3135545867089462 1104 8 0.3583448241213105 1104 9 0.3939167219260824 1104 10-11 0.47013391530806103 1104 12-13 0.3721475916399868 1104 14-15 0.637167708972342 1104 16-17 0.6837780962052022 1104 18-19 0.6049524368483432 1104 20-21 0.6451503624047916 1104 22-23 0.5980375772900786 1104 24-25 0.6814659216460868 1104 26-27 0.7810950872690938 1104 28-29 0.6795275648304582 1104 30-31 0.8297801260200757 1104 32-33 0.8958262364209162 1104 34-35 1.0314717318238138 1104 36-37 1.208120127399262 1104 38-39 1.1311376208566841 1104 40-41 1.1614121431132673 1104 42-43 1.1707967277825801 1104 44-45 1.3128983090529758 1104 46-47 1.3600016330756972 1104 48-49 1.4657363566384234 1104 50-51 1.4372186943994265 1104 52-53 1.5401166051037123 1104 54-55 1.6675769363322104 1104 56-57 1.8686113213808326 1104 58-59 2.1321926033054055 1104 60-61 2.177990900373125 1104 62-63 2.367528740329984 1104 64-65 2.4386913111441295 1104 66-67 2.5740616098621594 1104 68-69 2.653069486574765 1104 70-71 2.8658893793602047 1104 72-73 3.05452335293581 1104 74-75 3.16957932514277 1104 76-77 3.2682595985255283 1104 78-79 3.4084233875902967 1104 80-81 3.821794284452313 1104 82-83 3.995411627022353 1104 84-85 4.161461896243896 1104 86-87 4.351784693322212 1104 88-89 4.468010528545356 1104 90-91 4.467558837202986 1104 92-93 4.557457218154827 1104 94-95 4.722006799681505 1104 96-97 4.657793007515874 1104 98-99 4.672695578250316 1104 100-101 3.7156413224493487 1105 1 0.3554200157902798 1105 2 0.4057623427780186 1105 3 0.36971673259034077 1105 4 0.2643839594529922 1105 5 0.19974011491623855 1105 6 0.27682146144327646 1105 7 0.39181237029266214 1105 8 0.351096838055561 1105 9 0.46272665007761304 1105 10-11 0.44834924298060486 1105 12-13 0.38418308042727745 1105 14-15 0.5629179811256222 1105 16-17 0.6496892653756774 1105 18-19 0.5344514570965515 1105 20-21 0.5344360144840437 1105 22-23 0.5826757222933452 1105 24-25 0.6466860392163056 1105 26-27 0.7441079329037521 1105 28-29 0.8201591781550874 1105 30-31 0.8453564377988627 1105 32-33 0.7236076429090446 1105 34-35 0.8282285356557324 1105 36-37 0.9868762780633134 1105 38-39 1.000713279467611 1105 40-41 1.086573911020956 1105 42-43 1.053505414915172 1105 44-45 1.2025719428435266 1105 46-47 1.1754233617933139 1105 48-49 1.2938336786501807 1105 50-51 1.4214076776347966 1105 52-53 1.4569639815461173 1105 54-55 1.5457302130576593 1105 56-57 1.797958588961933 1105 58-59 1.7721682183718102 1105 60-61 1.823244329504412 1105 62-63 1.840249402206755 1105 64-65 1.8514067476779879 1105 66-67 2.077064222533611 1105 68-69 2.2809948077156292 1105 70-71 2.622424323840935 1105 72-73 2.6307153842878677 1105 74-75 2.695467415715381 1105 76-77 2.8643090215605795 1105 78-79 3.0790968036582242 1105 80-81 3.287596374589473 1105 82-83 3.487614979950724 1105 84-85 3.65239614401224 1105 86-87 3.893824318839304 1105 88-89 3.8563462568275284 1105 90-91 4.129156703521296 1105 92-93 4.180611801216006 1105 94-95 4.107474467872233 1105 96-97 4.217461633686131 1105 98-99 4.156344609384654 1105 100-101 3.5184634430676827 1106 1 0.3254047329305507 1106 2 0.26416400507966387 1106 3 0.30483327760713763 1106 4 0.24037866646809647 1106 5 0.26987596960435667 1106 6 0.2945284406158919 1106 7 0.18246720806816796 1106 8 0.15520566554526027 1106 9 0.21130183519468915 1106 10-11 0.13602064669964165 1106 12-13 0.1910051968179829 1106 14-15 0.4233262850241317 1106 16-17 0.4199916884382091 1106 18-19 0.25821133652472383 1106 20-21 0.35083644654718427 1106 22-23 0.34094696240335054 1106 24-25 0.3498413261768363 1106 26-27 0.5543442782076013 1106 28-29 0.5645534547981015 1106 30-31 0.48818142051845115 1106 32-33 0.4883828707251183 1106 34-35 0.5361707609500286 1106 36-37 0.7652431047455366 1106 38-39 0.8246424752256161 1106 40-41 1.008285929521037 1106 42-43 0.7431365336078244 1106 44-45 0.997438762127409 1106 46-47 1.0150695942407495 1106 48-49 0.9445810168325934 1106 50-51 1.159678241325004 1106 52-53 0.9862007474985361 1106 54-55 1.0803730528370608 1106 56-57 0.9906048488889283 1106 58-59 1.383338234373369 1106 60-61 1.69267374826957 1106 62-63 1.5137358600710833 1106 64-65 1.7232414729564276 1106 66-67 2.097307564554715 1106 68-69 1.9365419784841507 1106 70-71 2.296132097806808 1106 72-73 2.5931964920943855 1106 74-75 2.905022690838564 1106 76-77 2.7922563622795735 1106 78-79 3.0645366561987117 1106 80-81 3.17835415500247 1106 82-83 3.1254116270223555 1106 84-85 3.1216334172794937 1106 86-87 3.3059723955875846 1106 88-89 3.400819590034029 1106 90-91 3.510429387364802 1106 92-93 3.604502525598196 1106 94-95 3.5663077705552944 1106 96-97 3.5883884767715344 1106 98-99 3.3691357077001562 1106 100-101 2.686210901737377 1107 1 0.6172059558954004 1107 2 0.4870973970187862 1107 3 0.4610284693471698 1107 4 0.3950457985336513 1107 5 0.3524204949190377 1107 6 0.2997736584268793 1107 7 0.32132112021982095 1107 8 0.40135858355318277 1107 9 0.36210314003526634 1107 10-11 0.3467885979534202 1107 12-13 0.34729583842205614 1107 14-15 0.714616000139678 1107 16-17 0.8092907460054661 1107 18-19 0.5878059654768393 1107 20-21 0.6245862256981738 1107 22-23 0.6558951005923461 1107 24-25 0.720403338423715 1107 26-27 0.7399401826974099 1107 28-29 0.7086846886653504 1107 30-31 0.8244902902455564 1107 32-33 0.7691063074373332 1107 34-35 0.8776696901016621 1107 36-37 1.1070624265559346 1107 38-39 0.9773564402086592 1107 40-41 0.9808431240276079 1107 42-43 1.027543731777257 1107 44-45 1.0724593387911128 1107 46-47 1.4195098443495553 1107 48-49 1.2917776349340286 1107 50-51 1.3483283260017416 1107 52-53 1.3665391528178716 1107 54-55 1.504491272772512 1107 56-57 1.525083580590774 1107 58-59 1.7295596694394533 1107 60-61 1.683440966951018 1107 62-63 2.1068434318524005 1107 64-65 2.0761045379528262 1107 66-67 2.113991037292248 1107 68-69 2.44706904272212 1107 70-71 2.774484585751683 1107 72-73 3.019436801670828 1107 74-75 2.9585868706376672 1107 76-77 3.0821765980816735 1107 78-79 3.2545232544345026 1107 80-81 3.357816920936994 1107 82-83 3.471661072206551 1107 84-85 3.5080801829727015 1107 86-87 3.7733994292298902 1107 88-89 4.04768030218051 1107 90-91 4.105263675196774 1107 92-93 4.381038665114438 1107 94-95 4.5053237104671275 1107 96-97 4.817476984435533 1107 98-99 4.605074628405664 1107 100-101 3.7380789611533007 1108 1 0.40594039653696967 1108 2 0.3708087594351781 1108 3 0.29535994308578495 1108 4 0.2838697640416825 1108 5 0.32465058740393005 1108 6 0.17371196502193698 1108 7 0.29238032883448284 1108 8 0.24053607723710257 1108 9 0.21231780962152413 1108 10-11 0.4045918804111679 1108 12-13 0.43587725400119126 1108 14-15 0.48489283926919313 1108 16-17 0.6349745611950013 1108 18-19 0.5343766380720112 1108 20-21 0.5300017106939379 1108 22-23 0.5293230973444594 1108 24-25 0.4987548407487381 1108 26-27 0.5732858871784501 1108 28-29 0.45518154171693226 1108 30-31 0.723190957653884 1108 32-33 0.7410562386869373 1108 34-35 0.8527869368997258 1108 36-37 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4.173058837202987 2311 92-93 4.20545721815483 2311 94-95 4.036340133014843 2311 96-97 4.074626340849203 2311 98-99 3.90130668936143 2311 100-101 4.046863544671572 2312 1 -0.23987093444521435 2312 2 -0.17449713810955814 2312 3 0.016338211949197046 2312 4 0.02026489136320464 2312 5 -0.21885368389873605 2312 6 -0.009143392314911125 2312 7 -0.07398742317572982 2312 8 0.012309678596871265 2312 9 0.06589255937802818 2312 10-11 -0.08929991225921441 2312 12-13 -0.04302923427983529 2312 14-15 -0.04993443270804221 2312 16-17 2.0313739135957576E-4 2312 18-19 -0.01710522377768342 2312 20-21 0.0492321855788731 2312 22-23 0.022403859552568406 2312 24-25 0.09136817315624768 2312 26-27 0.09534330253543288 2312 28-29 0.10508330343562733 2312 30-31 0.10944734183556193 2312 32-33 0.14102118425176968 2312 34-35 0.3056551475294711 2312 36-37 0.26765829324219936 2312 38-39 0.30566645116969937 2312 40-41 0.5201688701863105 2312 42-43 0.5128379139440327 2312 44-45 0.5610515215735674 2312 46-47 0.38290333543703525 2312 48-49 0.5581839129261752 2312 50-51 0.6628419783093733 2312 52-53 0.8096168796781242 2312 54-55 0.9723688089296871 2312 56-57 1.3567628864549661 2312 58-59 1.2729811810099676 2312 60-61 1.2904856834593446 2312 62-63 1.4496715190230027 2312 64-65 1.4412141008201367 2312 66-67 1.6560643556062509 2312 68-69 1.7859728363825624 2312 70-71 2.0079569246649847 2312 72-73 2.5068297779769964 2312 74-75 2.195986793566714 2312 76-77 2.2559229702992774 2312 78-79 2.6848599609016617 2312 80-81 2.968424158148082 2312 82-83 3.2751645100536564 2312 84-85 3.6952987990445543 2312 86-87 4.097987878385364 2312 88-89 4.109318601033003 2312 90-91 4.313326547252409 2312 92-93 4.373137064393159 2312 94-95 4.375351665140048 2312 96-97 4.446055500651511 2312 98-99 4.283788384400783 2312 100-101 4.044479323547646 2313 1 -0.4759155717229504 2313 2 -0.2992894320367263 2313 3 -0.3450540815045713 2313 4 -0.20558439569013842 2313 5 -0.15255491143570765 2313 6 -0.2874828443969406 2313 7 -0.19052443627955995 2313 8 -0.2143994287522517 2313 9 -0.3170487953153014 2313 10-11 -0.23062614216320299 2313 12-13 -0.30983804054391584 2313 14-15 -0.291299245050638 2313 16-17 -0.163980524484451 2313 18-19 -0.2181682528068265 2313 20-21 -0.3009408732273897 2313 22-23 -0.22116716408923054 2313 24-25 -0.20799959559528958 2313 26-27 -0.08502057365044635 2313 28-29 -0.03688838344539391 2313 30-31 -0.06610852340521234 2313 32-33 -0.02030163714231037 2313 34-35 0.1673021915939259 2313 36-37 0.1437975699279903 2313 38-39 0.09594293694863865 2313 40-41 0.12924044771096987 2313 42-43 0.2435079346791369 2313 44-45 0.4110656941104551 2313 46-47 0.43806054112167203 2313 48-49 0.6126070462935971 2313 50-51 0.7899270277327659 2313 52-53 0.9065102832646303 2313 54-55 1.0065151547230187 2313 56-57 1.2286853156337045 2313 58-59 1.34510711479966 2313 60-61 1.3954521072696835 2313 62-63 1.427183194352974 2313 64-65 1.6839772306843592 2313 66-67 1.8094452305518054 2313 68-69 1.9383848601379832 2313 70-71 2.13899282763607 2313 72-73 2.6083487839702926 2313 74-75 2.79722012974047 2313 76-77 2.835659023812884 2313 78-79 3.0263522669006413 2313 80-81 3.053942991348862 2313 82-83 3.3780337534591354 2313 84-85 3.581439626128951 2313 86-87 3.6784168772302586 2313 88-89 3.6440299250970796 2313 90-91 3.953935273984598 2313 92-93 4.262436384821495 2313 94-95 3.997855937612538 2313 96-97 4.454651484527364 2313 98-99 4.582250175951469 2313 100-101 4.070079540840156 2314 1 -0.26109453001867067 2314 2 -0.21905495830500854 2314 3 -0.230950114181951 2314 4 -0.047374429992359524 2314 5 -0.09519629525949824 2314 6 -0.09466656438249998 2314 7 -0.11608241154585386 2314 8 -0.20847891060818569 2314 9 -0.04363301629381766 2314 10-11 -0.10094287352266207 2314 12-13 -0.2239798080109736 2314 14-15 -0.18895620027721804 2314 16-17 -0.03789729646495488 2314 18-19 -0.056340756868927144 2314 20-21 -0.14354248227234478 2314 22-23 -0.06719278920206762 2314 24-25 0.09048162845237329 2314 26-27 -0.025191125645392276 2314 28-29 3.495543592606509E-4 2314 30-31 0.05135429704974115 2314 32-33 0.1171176849374902 2314 34-35 0.19760436707687035 2314 36-37 0.313263233856496 2314 38-39 0.37942906937326626 2314 40-41 0.5564348307223455 2314 42-43 0.6055977749029964 2314 44-45 0.7804044172554185 2314 46-47 0.806689242150739 2314 48-49 0.8976054665860644 2314 50-51 0.8661541219736009 2314 52-53 1.0899944410548414 2314 54-55 1.1860551213234842 2314 56-57 1.398126155586759 2314 58-59 1.6252781181396116 2314 60-61 1.7063312145092553 2314 62-63 1.8428620736633157 2314 64-65 1.8907366863622777 2314 66-67 2.045093023474724 2314 68-69 2.1653225406759873 2314 70-71 2.48921398669005 2314 72-73 2.7074029340876393 2314 74-75 2.9205287143225256 2314 76-77 3.158407940584862 2314 78-79 3.389371031569354 2314 80-81 3.7886686300020465 2314 82-83 4.013457002240504 2314 84-85 4.364988946854716 2314 86-87 4.602775967318724 2314 88-89 4.62828277985426 2314 90-91 4.658761280483965 2314 92-93 4.9032495392717586 2314 94-95 4.910284286592503 2314 96-97 4.810564386224424 2314 98-99 4.8241789988436885 2314 100-101 4.375470292780937 2315 1 -0.3585755963663395 2315 2 -0.23627509120244738 2315 3 -0.2859411423755418 2315 4 -0.20510097151803564 2315 5 -0.21843859957989764 2315 6 -0.11434363397476943 2315 7 -0.0681762427120205 2315 8 -0.13253154520576516 2315 9 -0.2257828085903526 2315 10-11 -0.27109352339825676 2315 12-13 -0.24874390549094727 2315 14-15 -0.24389697543036704 2315 16-17 -0.09338935293407502 2315 18-19 -0.3603365563702212 2315 20-21 -0.22196440024517727 2315 22-23 -0.2300443215101282 2315 24-25 -0.25845896098301324 2315 26-27 -0.1815329774153085 2315 28-29 -0.10239783944705039 2315 30-31 -0.13558346292098378 2315 32-33 -0.1798127828800773 2315 34-35 -0.07167067819182904 2315 36-37 -0.09049072288764393 2315 38-39 -0.02676065770356928 2315 40-41 -0.05298952616163888 2315 42-43 0.09275238766782223 2315 44-45 0.06417582600655436 2315 46-47 0.2799327754857117 2315 48-49 0.5443065183494298 2315 50-51 0.5445322050932262 2315 52-53 0.7080978257609871 2315 54-55 0.7336708330458208 2315 56-57 0.875365624980212 2315 58-59 1.0480256758145288 2315 60-61 1.2112621575457965 2315 62-63 1.292262177992999 2315 64-65 1.461663663778456 2315 66-67 1.6792149744943892 2315 68-69 2.0222447604401808 2315 70-71 2.307091778942887 2315 72-73 2.4976897587782716 2315 74-75 2.6755135974432385 2315 76-77 2.828090062792608 2315 78-79 3.1284583380336954 2315 80-81 3.384183955813814 2315 82-83 3.613622372979581 2315 84-85 3.9916178691181763 2315 86-87 4.275661584297694 2315 88-89 4.285534785196376 2315 90-91 4.496829051078834 2315 92-93 4.694275684508508 2315 94-95 4.786042793421725 2315 96-97 4.675269272513265 2315 98-99 4.551464488005141 2315 100-101 4.4623695436282755 2316 1 -0.6148794792919858 2316 2 -0.643343481256931 2316 3 -0.5406359780562937 2316 4 -0.43108160559141595 2316 5 -0.385400722710564 2316 6 -0.2521930139490962 2316 7 -0.32265841467418 2316 8 -0.19620565997274042 2316 9 -0.2190376349203902 2316 10-11 -0.3665562644983069 2316 12-13 -0.46008753975696237 2316 14-15 -0.43153076958920167 2316 16-17 -0.48749783740475294 2316 18-19 -0.48937121460393485 2316 20-21 -0.5101345615232802 2316 22-23 -0.41750806586344424 2316 24-25 -0.464241547233577 2316 26-27 -0.49423617137544085 2316 28-29 -0.4768956716840549 2316 30-31 -0.4569425572441048 2316 32-33 -0.27109976634534405 2316 34-35 -0.1727723898912572 2316 36-37 -0.06665926402536115 2316 38-39 -0.16843568481412774 2316 40-41 -0.19436517362255756 2316 42-43 -0.10566800250787622 2316 44-45 0.1472772855398361 2316 46-47 0.13832390140211004 2316 48-49 0.3945724562234858 2316 50-51 0.35975327254604395 2316 52-53 0.3089686106362137 2316 54-55 0.5745797025839323 2316 56-57 0.542141058586914 2316 58-59 0.6983240002625308 2316 60-61 0.8726506514104742 2316 62-63 0.9782355176467128 2316 64-65 1.3761698726932288 2316 66-67 1.5125657592397452 2316 68-69 1.621269348262178 2316 70-71 1.8438146905635229 2316 72-73 2.2628739753424476 2316 74-75 2.611030224174584 2316 76-77 3.0352270950677145 2316 78-79 3.24825118842017 2316 80-81 3.2212673134979504 2316 82-83 3.558641225915853 2316 84-85 3.8958097523988044 2316 86-87 4.032947210611287 2316 88-89 4.2216910264706655 2316 90-91 4.475956485889018 2316 92-93 4.608358324655519 2316 94-95 4.697622982254121 2316 96-97 5.0202936990788025 2316 98-99 5.020845647406613 2316 100-101 4.799710478742574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3605.0 3 950.0 4 1015.0 5 1028.0 6 1347.0 7 1683.0 8 2210.0 9 2866.0 10 3873.0 11 5073.0 12 7086.0 13 9786.0 14 11397.0 15 15036.0 16 19426.0 17 26230.0 18 25450.0 19 19595.0 20 20076.0 21 21721.0 22 23993.0 23 27452.0 24 31049.0 25 35055.0 26 38921.0 27 43731.0 28 48593.0 29 54423.0 30 60943.0 31 68960.0 32 79519.0 33 94187.0 34 117361.0 35 160388.0 36 179376.0 37 200007.0 38 122766.0 39 9400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.65972615425654 18.701573065376202 14.437989616912905 21.200711163454354 2 15.678770417400115 20.76042748030351 40.10273268438975 23.45806941790663 3 19.124633591974295 26.33052747080492 29.985860960890353 24.558977976330436 4 12.264517071513078 17.04718655621088 36.8006986978414 33.887597674434645 5 12.953707419856519 38.51289319219385 32.9667511162394 15.566648271710228 6 30.651823433838484 36.37003820754983 17.295923838237776 15.682214520373911 7 27.881437326967056 35.005128295149056 19.89979135738073 17.21364302050315 8 27.314685656644432 33.59935853028706 19.28929268434888 19.796663128719626 9 27.97513811461946 16.483036289725977 20.160471257363398 35.38135433829117 10-11 25.374278220817647 27.173973350318647 25.75221607876856 21.69953235009515 12-13 26.788325538050085 25.17756851735813 27.051006887705604 20.983099056886186 14-15 23.291023782470713 25.692496963523098 26.931410884300433 24.085068369705755 16-17 21.228807741713837 29.530204708297102 27.096499966459763 22.144487583529298 18-19 21.10276790600667 26.650240067403182 31.39814578570278 20.848846240887376 20-21 22.34977832072976 25.44084284204643 31.644226009719844 20.565152827503965 22-23 21.785224528304845 25.773746181851237 31.606263252835497 20.83476603700842 24-25 21.387387618988203 26.064776482604827 31.81439549149812 20.733440406908848 26-27 21.06561159450821 26.29103389297049 31.810475735472068 20.83287877704923 28-29 20.771613810043412 26.656846921371923 31.904496655508435 20.667042613076227 30-31 20.860805079963786 26.22497448231066 31.82773741482609 21.08648302289946 32-33 20.670009987329323 26.481782998468358 31.719080814609963 21.129126199592356 34-35 21.400428143356866 26.447899141487618 31.362958546456753 20.788714168698768 36-37 21.455534545928746 26.183045398348376 31.34650252408096 21.01491753164192 38-39 21.027360165284286 26.362460760026877 31.40520474991726 21.20497432477158 40-41 21.064244082823084 26.42232100071676 31.467054955366336 21.046379961093823 42-43 21.154940056657296 26.39807469247951 30.98769792679682 21.459287324066377 44-45 20.97696286379044 26.595281254387547 30.940991234931904 21.486764646890105 46-47 21.422027679320557 26.961402087937337 30.319126763415056 21.297443469327053 48-49 21.40171892722343 26.99652209178456 30.109088538573374 21.492670442418632 50-51 21.26153337969511 27.287801640128777 29.89789231511257 21.55277266506354 52-53 21.563902177925666 27.383393011258477 29.588645378998173 21.464059431817684 54-55 21.17447748387497 27.269428223485914 29.680175694029646 21.875918598609463 56-57 21.440531708890134 27.532099686642 29.422025441573414 21.605343162894453 58-59 21.645835474740586 27.011314151338368 29.522025365961273 21.82082500795977 60-61 21.457508021862964 27.1613275850236 29.936411125530288 21.44475326758315 62-63 21.253693250549365 27.207708285542015 29.938739362122195 21.59985910178642 64-65 21.15336823450327 27.240713086998557 29.78788215919258 21.818036519305593 66-67 21.3053526855875 27.349448373009842 29.39569384662874 21.94950509477392 68-69 21.26378229003739 27.17591973076449 29.599640985897352 21.960656993300766 70-71 21.37045919544265 27.359945891461983 29.429979840933935 21.839615072161433 72-73 21.487707170978858 27.384115570081 29.219624899944275 21.908552358995863 74-75 21.256833649447728 27.815570127879035 29.053305286276792 21.874290936396445 76-77 21.45719057287849 27.497421590819705 29.072891494336588 21.97249634196522 78-79 21.596156883588346 27.54184331196458 28.901688273593585 21.960311530853488 80-81 21.660772235741717 27.911303047919606 28.729231728700526 21.69869298763815 82-83 21.793525781854765 27.738473929817193 28.61542993785277 21.85257035047527 84-85 21.650082544553374 27.57972099063492 28.64067234011615 22.12952412469555 86-87 21.90841577255018 27.440515753152717 28.55807910592425 22.09298936837286 88-89 21.843025449876944 27.494273495593696 28.39117510252543 22.27152595200393 90-91 21.92371522733577 27.466780753758368 28.497889858942592 22.111614159963267 92-93 22.257247230732972 27.19651516139223 28.415262184022744 22.13097542385205 94-95 22.036734159106967 27.01106011756586 28.572377853184978 22.379827870142197 96-97 22.2084489737711 26.86047768968819 28.40738239152 22.52369094502071 98-99 22.23135312817629 27.028087565179153 27.87312470362427 22.867434603020282 100-101 22.959339728207897 25.98990418354296 28.232178054288777 22.818578033960364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5780.0 1 3653.5 2 2159.5 3 2702.5 4 2046.0 5 1889.5 6 3738.5 7 7716.0 8 11902.0 9 12415.0 10 8731.5 11 5477.0 12 4750.0 13 4701.0 14 4557.5 15 4368.5 16 4301.0 17 4349.5 18 4434.5 19 4582.5 20 4874.5 21 5259.0 22 5928.5 23 6702.0 24 7569.0 25 8799.5 26 10355.0 27 12149.0 28 13809.0 29 15701.0 30 18091.5 31 20388.0 32 23110.5 33 25822.0 34 27890.0 35 29830.0 36 32638.5 37 35759.5 38 37613.5 39 38087.0 40 38935.5 41 41596.0 42 44229.5 43 47028.0 44 49928.0 45 52321.5 46 53422.0 47 55164.0 48 59807.0 49 60177.0 50 56091.0 51 52641.5 52 50531.5 53 50171.0 54 50109.0 55 47554.5 56 43583.0 57 40466.0 58 37400.0 59 33494.5 60 29211.5 61 25203.5 62 21022.0 63 16775.5 64 13154.5 65 10512.5 66 8174.0 67 6382.0 68 4820.0 69 3307.5 70 2423.5 71 1913.5 72 1551.0 73 1272.5 74 1016.0 75 835.5 76 604.0 77 375.5 78 235.0 79 154.0 80 88.0 81 46.5 82 31.5 83 18.5 84 12.5 85 12.0 86 7.5 87 5.0 88 4.5 89 2.5 90 1.5 91 2.5 92 1.0 93 1.0 94 1.5 95 2.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38036396864582533 2 0.023126430125277563 3 0.043996623165162195 4 0.038230683946935816 5 0.05872483747258829 6 0.05427503655417445 7 0.03164999244787309 8 0.030709893662292702 9 0.03290345749531361 10-11 0.02967578499815427 12-13 0.03061588378373466 14-15 0.024411231798904098 16-17 0.02995781463382839 18-19 0.02506930094881037 20-21 0.03713390203042535 22-23 0.025037964322624354 24-25 0.04534409809116075 26-27 0.03854405020879594 28-29 0.030803903540850736 30-31 0.03791731768507568 32-33 0.03484632831884641 34-35 0.034501625430800265 36-37 0.033906229533266025 38-39 0.01660841187858687 40-41 0.012565987100591198 42-43 0.012252620838731066 44-45 0.009589007612919965 46-47 0.016953114766633012 48-49 0.013882125400403742 50-51 0.009620344239105977 52-53 0.0042617811612977625 54-55 0.00476316718027397 56-57 0.007364107153713045 58-59 0.0033216823757173737 60-61 0.005859949096784423 62-63 0.006204651984830566 64-65 0.007489453658457097 66-67 0.004355791039855801 68-69 0.0060479688539005014 70-71 0.01660841187858687 72-73 0.013537422512357598 74-75 0.010215740136640224 76-77 0.0073014339013410205 78-79 0.006329998489574619 80-81 0.009056284967757745 82-83 0.010779799407988459 84-85 0.004700493927901944 86-87 4.700493927901944E-4 88-89 0.022750390611045408 90-91 0.019084005347281892 92-93 0.013098709745753417 94-95 0.024191875415602006 96-97 0.018457272823561632 98-99 0.016451728747656803 100-101 0.10958418177248733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1595577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.9411263412355 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37695261633918 34.44396803298428 2 13.993044291154206 13.136985199391473 3 5.097325990123329 7.1782266991452754 4 2.356676556746014 4.4250020798257 5 1.3347070955912954 3.1326327201347244 6 0.8178246686230036 2.303376665688688 7 0.5572130319915808 1.8309345133589787 8 0.38186366488403767 1.434008843075906 9 0.27869644653914416 1.1774092597062615 >10 1.5811531097116447 13.778317165914084 >50 0.14233914053595054 4.602105370990715 >100 0.07562185083272503 6.375174973014863 >500 0.004566780725528939 1.478332184613573 >1k 0.0014774878817887744 1.4252441264635058 >5k 1.3431708016261584E-4 0.32685094233960693 >10k+ 4.0295124048784754E-4 2.951431223352496 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20582 1.2899408803210375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15366 0.9630371959485502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10863 0.6808195405173176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5184 0.32489814029658237 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3637 0.22794261887705827 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3304 0.2070724258371736 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2558 0.1603181795676423 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 0.16000481330578217 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2385 0.14947570690728182 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2022 0.12672531629623643 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1688 0.10579245000397976 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2534650474405184E-4 0.0 12-13 0.0 0.0 0.0 1.2534650474405184E-4 0.0 14-15 0.0 0.0 0.0 1.2534650474405184E-4 0.0 16-17 0.0 0.0 0.0 1.566831309300648E-4 0.0 18-19 0.0 0.0 0.0 2.1935638330209073E-4 0.0 20-21 0.0 0.0 0.0 2.8202963567411664E-4 0.0 22-23 0.0 0.0 0.0 5.953958975342462E-4 0.0 24-25 0.0 0.0 0.0 0.0015354946831146348 0.0 26-27 0.0 0.0 0.0 0.003697721889949529 0.0 28-29 0.0 0.0 0.0 0.010121730258082186 0.0 30-31 0.0 0.0 0.0 0.02729420140801729 0.0 32-33 0.0 0.0 0.0 0.060417015286632986 0.0 34-35 0.0 0.0 0.0 0.1095528451463013 0.0 36-37 0.0 0.0 0.0 0.18664094556389318 0.0 38-39 0.0 0.0 0.0 0.30233576944265306 0.0 40-41 0.0 0.0 0.0 0.42483064120377767 0.0 42-43 0.0 0.0 0.0 0.5446932363652772 0.0 44-45 0.0 0.0 0.0 0.6821043421909441 0.0 46-47 0.0 3.133662618601296E-5 0.0 0.8446160855916073 0.0 48-49 0.0 6.267325237202592E-5 0.0 1.015369361679192 0.0 50-51 0.0 6.267325237202592E-5 0.0 1.1925779827610952 0.0 52-53 0.0 6.267325237202592E-5 0.0 1.3643340308866323 0.0 54-55 0.0 6.267325237202592E-5 0.0 1.5461177993916935 0.0 56-57 0.0 6.267325237202592E-5 0.0 1.751090671274404 0.0 58-59 0.0 6.267325237202592E-5 0.0 1.9685355203791481 0.0 60-61 0.0 6.267325237202592E-5 0.0 2.199423782117692 0.0 62-63 0.0 6.267325237202592E-5 0.0 2.4261129359473093 0.0 64-65 0.0 6.267325237202592E-5 0.0 2.650326496308232 0.0 66-67 0.0 6.267325237202592E-5 0.0 2.9022416342175905 0.0 68-69 0.0 6.267325237202592E-5 0.0 3.1755596878119956 0.0 70-71 0.0 6.267325237202592E-5 0.0 3.4707193698580516 0.0 72-73 0.0 6.267325237202592E-5 0.0 3.7591730139003006 0.0 74-75 0.0 6.267325237202592E-5 0.0 4.044743688333437 0.0 76-77 0.0 6.267325237202592E-5 0.0 4.338555895453494 0.0 78-79 0.0 6.267325237202592E-5 0.0 4.663391362497705 0.0 80-81 0.0 6.267325237202592E-5 0.0 5.010507170760171 0.0 82-83 0.0 6.267325237202592E-5 0.0 5.350697584635527 0.0 84-85 0.0 6.267325237202592E-5 0.0 5.69248616644637 0.0 86-87 0.0 6.267325237202592E-5 0.0 6.04530524067469 0.0 88-89 0.0 6.267325237202592E-5 0.0 6.410063569479881 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9580 0.0 38.169876 1 GTATCAA 14980 0.0 34.62645 1 TATCAAC 18315 0.0 28.409634 2 ATCAACG 18340 0.0 28.319088 3 TCAACGC 18495 0.0 28.081757 4 CAACGCA 18655 0.0 27.815435 5 AACGCAG 19110 0.0 27.22349 6 GTACATG 11760 0.0 25.74289 1 GAGTACT 11260 0.0 25.354692 12-13 TACATGG 11850 0.0 24.821657 2 ACGCAGA 21275 0.0 24.424698 7 CGCAGAG 21470 0.0 24.114368 8 GTACTTT 11900 0.0 23.854357 14-15 GCAGAGT 21830 0.0 23.238008 9 ACATGGG 11985 0.0 23.114737 3 AGTACTT 11470 0.0 22.177794 12-13 ACTTTTT 13110 0.0 21.778849 16-17 AGAGTAC 18035 0.0 21.22857 10-11 GTATAGA 340 1.8189894E-12 21.033096 1 CATGGGA 7810 0.0 20.868996 4 >>END_MODULE