FastQCFastQC Report
Thu 26 May 2016
SRR938023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595577
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194221.2172399075694875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155230.9728768965709584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109360.6853946879404754No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47570.2981366615337273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37730.2364661811996538No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.21020608845577493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25160.1576859029680172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24120.15116788472132653No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20220.12672531629623643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.1213980898446142No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.10817403359411673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.039.7894481
GTATCAA137400.034.612711
TATCAAC170700.027.8047752
ATCAACG172100.027.520733
TCAACGC173050.027.3687884
CAACGCA175850.026.9591985
AACGCAG179700.026.3501956
GTACATG111100.026.2402971
TACATGG113200.025.2073482
ACATGGG114950.023.8284053
GAGTACT112500.023.6202412-13
ACGCAGA200950.023.4455477
CGCAGAG202500.023.2191818
CATGGGA76100.022.370014
GTACTTT117850.022.28600714-15
GCAGAGT209900.022.1969499
ATGGGAT29000.022.1355325
AGTACTT114000.021.78881312-13
AGAGTAC177600.020.69830310-11
ATACCGT2309.038558E-820.649476
Produced by FastQC (version 0.11.5)