##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938023_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1595577 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50414489554562 33.0 31.0 34.0 31.0 34.0 2 32.713178994182044 34.0 31.0 34.0 31.0 34.0 3 32.761729455864554 34.0 31.0 34.0 31.0 34.0 4 36.22814129308708 37.0 35.0 37.0 35.0 37.0 5 36.16818241927528 37.0 35.0 37.0 35.0 37.0 6 36.23697132761377 37.0 37.0 37.0 35.0 37.0 7 36.20589228849501 37.0 37.0 37.0 35.0 37.0 8 36.21127717433881 37.0 37.0 37.0 35.0 37.0 9 37.88645988253779 39.0 38.0 39.0 35.0 39.0 10-11 37.87199520925659 39.0 38.0 39.0 35.0 39.0 12-13 37.79123758991261 39.0 38.0 39.0 35.0 39.0 14-15 38.9885317349147 40.0 38.0 41.0 35.5 41.0 16-17 38.9161015106134 40.0 38.0 41.0 35.5 41.0 18-19 39.20213816067792 40.5 39.0 41.0 36.0 41.0 20-21 39.19883966740558 41.0 39.0 41.0 36.0 41.0 22-23 39.08097916929111 40.0 39.0 41.0 35.0 41.0 24-25 38.962992384573106 40.0 38.0 41.0 35.0 41.0 26-27 38.79023701143849 40.0 38.0 41.0 35.0 41.0 28-29 38.655873079143156 40.0 38.0 41.0 35.0 41.0 30-31 38.36322534105217 40.0 38.0 41.0 34.0 41.0 32-33 38.075476771099105 40.0 37.0 41.0 34.0 41.0 34-35 37.923474078656184 40.0 37.0 41.0 33.0 41.0 36-37 37.84028849751532 40.0 37.0 41.0 33.0 41.0 38-39 37.65179837764019 40.0 36.5 41.0 33.0 41.0 40-41 37.41628702344042 40.0 36.0 41.0 33.0 41.0 42-43 37.12404070753088 40.0 36.0 41.0 32.5 41.0 44-45 36.77932309127043 39.5 35.0 41.0 31.0 41.0 46-47 36.2857862704213 39.0 35.0 41.0 30.5 41.0 48-49 35.78615008865132 39.0 35.0 41.0 28.5 41.0 50-51 35.39160943031894 39.0 35.0 40.0 27.5 41.0 52-53 34.88836702960747 38.0 34.5 40.0 26.0 41.0 54-55 34.53393568596188 38.0 34.0 40.0 25.0 41.0 56-57 34.174503643509524 38.0 34.0 40.0 24.0 41.0 58-59 33.71065106854762 37.0 33.0 40.0 22.5 41.0 60-61 33.263499975244066 36.5 33.0 39.5 21.5 41.0 62-63 32.75600770128925 36.0 32.0 39.0 19.5 40.5 64-65 32.23089484243005 35.0 31.5 39.0 17.5 40.0 66-67 31.70897205211657 35.0 31.0 38.0 12.5 40.0 68-69 31.143025062406892 35.0 30.5 38.0 7.5 40.0 70-71 30.566239360431993 35.0 29.5 37.0 6.0 39.0 72-73 29.944619407273983 34.0 29.0 36.5 2.0 39.0 74-75 29.3277522802096 34.0 28.5 36.0 2.0 39.0 76-77 27.99735800904626 32.5 26.5 34.5 2.0 36.5 78-79 28.693957421045802 33.5 28.0 35.0 2.0 37.0 80-81 28.43334856293366 34.0 28.0 35.0 2.0 37.0 82-83 27.916970475257543 33.5 26.5 35.0 2.0 36.0 84-85 27.307213628674766 33.0 26.0 35.0 2.0 36.0 86-87 26.63412984769773 33.0 25.0 35.0 2.0 35.0 88-89 25.88021480630518 32.0 23.5 35.0 2.0 35.0 90-91 25.269737217320127 32.0 20.0 34.0 2.0 35.0 92-93 24.37468765217849 31.0 14.5 34.0 2.0 35.0 94-95 23.519581630971118 31.0 4.5 34.0 2.0 35.0 96-97 22.519213112247165 30.5 2.0 34.0 2.0 35.0 98-99 21.161757470808368 29.5 2.0 34.0 2.0 35.0 100-101 17.259139483710282 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2557747801237866 1101 2 0.18760536492694513 1101 3 0.21750755139581202 1101 4 0.1385896468520471 1101 5 -0.015758034272948862 1101 6 0.019090928438920685 1101 7 0.164093402667973 1101 8 0.11479169863203253 1101 9 0.13685971407535646 1101 10-11 0.18712042744502355 1101 12-13 0.1759821260753398 1101 14-15 0.40839173017174346 1101 16-17 0.4173102268090574 1101 18-19 0.32551674685703347 1101 20-21 0.20977102440598827 1101 22-23 0.2781062532002423 1101 24-25 0.32160725139542734 1101 26-27 0.4358911177897937 1101 28-29 0.3760524721007883 1101 30-31 0.5620078155418042 1101 32-33 0.5820236893199109 1101 34-35 0.5663125026576026 1101 36-37 0.6779466759588431 1101 38-39 0.7199546932749996 1101 40-41 0.6811498592804313 1101 42-43 0.8550405390849392 1101 44-45 0.8585100385080509 1101 46-47 0.9542812242512042 1101 48-49 0.9304496142441749 1101 50-51 0.9782369637156663 1101 52-53 1.142300268267789 1101 54-55 1.284393808917109 1101 56-57 1.2630322036063504 1101 58-59 1.4720744021782863 1101 60-61 1.432675326605505 1101 62-63 1.3379250963850993 1101 64-65 1.403771376846123 1101 66-67 1.7249450117270193 1101 68-69 1.9932272660381365 1101 70-71 2.0182347223990185 1101 72-73 2.0289403371068495 1101 74-75 2.0209120923046946 1101 76-77 2.1418353111650283 1101 78-79 2.123631845847214 1101 80-81 2.217941465905291 1101 82-83 2.4301630338994507 1101 84-85 2.6307547164212934 1101 86-87 2.6220943289776137 1101 88-89 3.1852079135963507 1101 90-91 3.476968211179358 1101 92-93 3.4835742217421064 1101 94-95 3.8020673031682755 1101 96-97 4.092878178665448 1101 98-99 4.2545307999939865 1101 100-101 4.077597134024568 1102 1 0.05163615350706863 1102 2 0.08121774266667359 1102 3 0.08899018492860478 1102 4 -0.02515647556926126 1102 5 -0.14454450046385148 1102 6 -0.08160745950540615 1102 7 0.07908331672557267 1102 8 0.11494425910531447 1102 9 0.13635117916442852 1102 10-11 0.09607784612181547 1102 12-13 0.17696953136071158 1102 14-15 0.20367994233250641 1102 16-17 0.16897779753778508 1102 18-19 0.14635460050068616 1102 20-21 0.17908623977051974 1102 22-23 0.17406530766432837 1102 24-25 0.26871008562999776 1102 26-27 0.2624224435403164 1102 28-29 0.33262782849900674 1102 30-31 0.22479089997555235 1102 32-33 0.2298346584179356 1102 34-35 0.17248505702846018 1102 36-37 0.2052253361388665 1102 38-39 0.30209261641842744 1102 40-41 0.4783440179094143 1102 42-43 0.45359884261247885 1102 44-45 0.21265904771252764 1102 46-47 0.1415545024367546 1102 48-49 0.2437473783856845 1102 50-51 0.2885714525533274 1102 52-53 0.3036647521896114 1102 54-55 0.5003707044809858 1102 56-57 0.7334079261497024 1102 58-59 0.6289033903633197 1102 60-61 0.5002712701920302 1102 62-63 0.7606309682682095 1102 64-65 0.7570346877474208 1102 66-67 0.8032773986203843 1102 68-69 1.0700488804669739 1102 70-71 1.2537213479308598 1102 72-73 1.5580878630845092 1102 74-75 1.4592956717124217 1102 76-77 1.3503770025521398 1102 78-79 1.2970369532328334 1102 80-81 1.6081138168621827 1102 82-83 1.745114595949186 1102 84-85 1.7620563115257966 1102 86-87 1.7554756199782382 1102 88-89 1.9632758623360331 1102 90-91 2.091595158444285 1102 92-93 2.38932257324144 1102 94-95 2.5330131357246763 1102 96-97 2.927807746580285 1102 98-99 3.4665495988345256 1102 100-101 3.0019006530861496 1103 1 0.23150646413930787 1103 2 0.20971788185349993 1103 3 0.220564715377769 1103 4 0.15041746538278034 1103 5 0.031142018064365118 1103 6 0.12328372915004593 1103 7 0.17728780672868538 1103 8 0.19673698536577433 1103 9 0.24331215772966175 1103 10-11 0.29894263840594704 1103 12-13 0.25875816389113027 1103 14-15 0.5086249635380327 1103 16-17 0.43908005891426427 1103 18-19 0.34123559576833884 1103 20-21 0.34127061318420004 1103 22-23 0.44561163231349354 1103 24-25 0.4530497260367454 1103 26-27 0.44141976624085544 1103 28-29 0.4460671348573868 1103 30-31 0.42135646515591674 1103 32-33 0.4117467169091569 1103 34-35 0.5080000073305726 1103 36-37 0.49664979044464985 1103 38-39 0.4682049238084218 1103 40-41 0.653572038672813 1103 42-43 0.73235729616502 1103 44-45 0.8039359230502612 1103 46-47 0.9451076554276341 1103 48-49 1.1001024849045464 1103 50-51 1.2523920898652179 1103 52-53 1.2120511675558348 1103 54-55 1.27432925955803 1103 56-57 1.4135279543145671 1103 58-59 1.5153627641863707 1103 60-61 1.4797197219433258 1103 62-63 1.5150921105826356 1103 64-65 1.6418729444734197 1103 66-67 1.6784073745492556 1103 68-69 1.7162943159579704 1103 70-71 1.6758780477294444 1103 72-73 1.8429746305115167 1103 74-75 1.9211492717386207 1103 76-77 1.8951583154932408 1103 78-79 1.9168205177254585 1103 80-81 2.0208426563715918 1103 82-83 2.1867799662680056 1103 84-85 2.2542188653583466 1103 86-87 2.598821875852746 1103 88-89 2.7504561420196687 1103 90-91 2.955895171544224 1103 92-93 2.771487624249314 1103 94-95 3.131890020887365 1103 96-97 3.1897634851710563 1103 98-99 3.353568790240967 1103 100-101 3.323961559601731 1104 1 0.1487151515802907 1104 2 0.12024857118305476 1104 3 0.14477245853169052 1104 4 0.11469130863113008 1104 5 0.050218505316493633 1104 6 0.051429149357787196 1104 7 0.078728788787231 1104 8 0.10868221671610456 1104 9 0.09033576490624284 1104 10-11 0.19530713321237414 1104 12-13 0.1573105718231389 1104 14-15 0.36033601169667406 1104 16-17 0.391372787903876 1104 18-19 0.23249621899779527 1104 20-21 0.2827172609651285 1104 22-23 0.31713948878186926 1104 24-25 0.42179004709434764 1104 26-27 0.4301921930586161 1104 28-29 0.3137338015240516 1104 30-31 0.31526392502372147 1104 32-33 0.3702094175701589 1104 34-35 0.3327865984542768 1104 36-37 0.42662996041632084 1104 38-39 0.41656564146659747 1104 40-41 0.48631896973986244 1104 42-43 0.596941809857185 1104 44-45 0.5656214754581939 1104 46-47 0.6869254080244218 1104 48-49 0.8856548928365484 1104 50-51 0.9933835912816491 1104 52-53 0.8073892223244812 1104 54-55 0.9313283152709673 1104 56-57 0.9720322036063536 1104 58-59 0.9906403845829743 1104 60-61 1.1076479561265202 1104 62-63 1.2201714306959488 1104 64-65 1.1995924912156255 1104 66-67 1.0795461847475458 1104 68-69 1.2886163178465502 1104 70-71 1.4142425425358702 1104 72-73 1.6102824680941445 1104 74-75 1.5913891206526891 1104 76-77 1.4939203551532962 1104 78-79 1.347265276932255 1104 80-81 1.5085348187889664 1104 82-83 1.734811127741093 1104 84-85 2.036825097652965 1104 86-87 2.0467463328876825 1104 88-89 2.230843299715609 1104 90-91 2.4861529619124916 1104 92-93 3.023693478829106 1104 94-95 3.271185582738166 1104 96-97 3.3586709450388597 1104 98-99 3.707898346426049 1104 100-101 3.150067319752818 1105 1 0.19656013307386644 1105 2 0.19132325004218842 1105 3 0.15944217331505683 1105 4 0.1401143436844663 1105 5 0.0024928358194316047 1105 6 0.05383237165258237 1105 7 0.16153915238400884 1105 8 0.14308239089420027 1105 9 0.18331508125721285 1105 10-11 0.13614101085225627 1105 12-13 0.21847778279635577 1105 14-15 0.3812974747926887 1105 16-17 0.312768172184299 1105 18-19 0.2523492562283707 1105 20-21 0.2331507467042968 1105 22-23 0.27283924928698156 1105 24-25 0.30308941569875003 1105 26-27 0.289958141240632 1105 28-29 0.36450279782276596 1105 30-31 0.3236743321650195 1105 32-33 0.3881554223600503 1105 34-35 0.3712422919008205 1105 36-37 0.4389480531661576 1105 38-39 0.33525103227869835 1105 40-41 0.25338668147511356 1105 42-43 0.109909369186596 1105 44-45 0.19845948982788997 1105 46-47 0.3020636618890009 1105 48-49 0.5721506472454365 1105 50-51 0.5961660863828939 1105 52-53 0.6700876764938712 1105 54-55 0.7586853803700251 1105 56-57 0.8655795582765009 1105 58-59 0.9009957079010675 1105 60-61 0.9016601485933151 1105 62-63 1.2000956632237134 1105 64-65 1.1375415441222216 1105 66-67 1.2306702866417432 1105 68-69 1.5249753424492063 1105 70-71 1.6731841928733395 1105 72-73 1.7725657252245597 1105 74-75 1.775899680199828 1105 76-77 1.6824076183799441 1105 78-79 1.7084220372196555 1105 80-81 1.7443377798166146 1105 82-83 1.8450103439396557 1105 84-85 2.050201975510575 1105 86-87 2.147278882419581 1105 88-89 2.2827516384920052 1105 90-91 2.4942280762168707 1105 92-93 2.788809524986302 1105 94-95 2.8448023909245386 1105 96-97 3.347469987059327 1105 98-99 3.277031810392952 1105 100-101 3.4052726321847793 1106 1 0.07938181824695789 1106 2 0.038688700632889095 1106 3 0.061646244939453254 1106 4 0.02208289439164446 1106 5 -0.15836401895535346 1106 6 -0.016227808571009916 1106 7 0.06424335189402797 1106 8 0.043310048431315806 1106 9 0.007817965553492456 1106 10-11 0.016504544215607098 1106 12-13 0.041883387357117385 1106 14-15 -0.17077078441983673 1106 16-17 -0.06430035125469402 1106 18-19 -0.021209282620326064 1106 20-21 -0.10196558693130697 1106 22-23 -0.0923200581436987 1106 24-25 -0.08143325387652567 1106 26-27 -0.15697609173102478 1106 28-29 0.018478138093627194 1106 30-31 0.15305356903666478 1106 32-33 0.15512203892937748 1106 34-35 0.10188368583291663 1106 36-37 0.08876911899236717 1106 38-39 0.015873084832293216 1106 40-41 0.0249791639146153 1106 42-43 -0.02868925810398082 1106 44-45 -0.032679495415592896 1106 46-47 0.23964385462635818 1106 48-49 0.07845424558735914 1106 50-51 0.10775252332048524 1106 52-53 0.0956157595413103 1106 54-55 0.17845776510915812 1106 56-57 0.39830728451249797 1106 58-59 0.6226986370101599 1106 60-61 0.37261235742101917 1106 62-63 0.38391253101957545 1106 64-65 0.62127533911206 1106 66-67 0.7590575116083933 1106 68-69 0.8354156705973601 1106 70-71 0.7603493386523716 1106 72-73 1.1247517237575728 1106 74-75 1.4089975348921726 1106 76-77 1.3317391253798334 1106 78-79 1.243410908000218 1106 80-81 0.944000838206442 1106 82-83 1.4761865322718357 1106 84-85 1.5925532529927686 1106 86-87 1.7936654267388157 1106 88-89 1.944742976091014 1106 90-91 2.097017039582397 1106 92-93 1.8910527021300787 1106 94-95 2.2924994985957703 1106 96-97 2.673887773517823 1106 98-99 2.6136912266849492 1106 100-101 2.5978148925683513 1107 1 0.22239113915241404 1107 2 0.19169375538189826 1107 3 0.22676890771055724 1107 4 0.1242021830208273 1107 5 0.1304741644376648 1107 6 0.12160136418246736 1107 7 0.14291343148585156 1107 8 0.18546872359581812 1107 9 0.19554393179483753 1107 10-11 0.1935317226486788 1107 12-13 0.2532320099056946 1107 14-15 0.34686330863409154 1107 16-17 0.3719537910108457 1107 18-19 0.23422058650778865 1107 20-21 0.2770630221724062 1107 22-23 0.24701476618976415 1107 24-25 0.26778117004153046 1107 26-27 0.40223169594365515 1107 28-29 0.2717994917149156 1107 30-31 0.2523961931107195 1107 32-33 0.36690093998470985 1107 34-35 0.29144927044717406 1107 36-37 0.4348709724003399 1107 38-39 0.28325982699700347 1107 40-41 0.2616993514531316 1107 42-43 0.43482907128638715 1107 44-45 0.5378252038203755 1107 46-47 0.6559356166351691 1107 48-49 0.8773659447673126 1107 50-51 0.7617724061950994 1107 52-53 0.9085893998655408 1107 54-55 0.9982040365315044 1107 56-57 0.8672266110630744 1107 58-59 1.0152529012274485 1107 60-61 0.857052305882398 1107 62-63 1.0639716970075384 1107 64-65 1.2824819719080338 1107 66-67 1.4399203525238455 1107 68-69 1.6564534239273279 1107 70-71 1.6528572784435518 1107 72-73 1.7599304929498878 1107 74-75 1.5731010602887316 1107 76-77 1.612034869134657 1107 78-79 1.4942706031639474 1107 80-81 1.731882799259452 1107 82-83 1.9878271064361677 1107 84-85 1.9790088526463059 1107 86-87 2.2135310643568324 1107 88-89 2.209633801269092 1107 90-91 2.4817228687034394 1107 92-93 2.6981444331122866 1107 94-95 2.8185721783884112 1107 96-97 3.3696713888915006 1107 98-99 3.6220705612507267 1107 100-101 3.3411982562818885 1108 1 0.11568365373302214 1108 2 0.10494968153881956 1108 3 0.06910919091328793 1108 4 0.07414927373422842 1108 5 -0.056095566743330494 1108 6 0.04376044326216544 1108 7 0.06691550983867245 1108 8 0.11049504245822561 1108 9 0.10098928858627687 1108 10-11 0.1493633308103739 1108 12-13 0.11579255686732637 1108 14-15 0.2316290096572402 1108 16-17 0.21270272218894348 1108 18-19 0.23636773497332797 1108 20-21 0.2649699235529397 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2316 38-39 -0.46529189656936154 2316 40-41 -0.5269251519752132 2316 42-43 -0.4585941514152978 2316 44-45 -0.361505080558409 2316 46-47 -0.5467295020724023 2316 48-49 -0.33908577106385707 2316 50-51 -0.4681413049839094 2316 52-53 -0.17623595056625163 2316 54-55 -0.07892617988809292 2316 56-57 -0.11456115738949535 2316 58-59 -0.06529737475312203 2316 60-61 0.13775238212928542 2316 62-63 0.14731458422291865 2316 64-65 0.3354846073982003 2316 66-67 0.542641620432196 2316 68-69 0.8099572583721404 2316 70-71 0.7155689602398816 2316 72-73 0.7928889687857072 2316 74-75 1.0863088993525523 2316 76-77 1.195326994157444 2316 78-79 1.0273109200857888 2316 80-81 1.224455289051761 2316 82-83 1.3880372688199323 2316 84-85 1.639906010516036 2316 86-87 1.7486564297064895 2316 88-89 1.8639726219839332 2316 90-91 2.117140513779713 2316 92-93 2.1922211413463977 2316 94-95 2.674390285366105 2316 96-97 3.1672034484966716 2316 98-99 3.504603049053987 2316 100-101 3.882087375077848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 16.0 5 43.0 6 89.0 7 201.0 8 464.0 9 837.0 10 1432.0 11 2465.0 12 3872.0 13 6064.0 14 7588.0 15 10716.0 16 14911.0 17 21685.0 18 22008.0 19 15204.0 20 13178.0 21 13713.0 22 14481.0 23 16281.0 24 18419.0 25 20992.0 26 24157.0 27 27818.0 28 34002.0 29 41273.0 30 49774.0 31 60002.0 32 73725.0 33 93672.0 34 126178.0 35 185233.0 36 231820.0 37 284626.0 38 152301.0 39 6328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80604821954691 19.074165646659484 14.818275771084693 21.301510362708914 2 15.922446577185163 20.94131717459424 39.48201604799983 23.65422020022077 3 19.19689583550084 26.064189168015222 30.24391833705831 24.49499665942562 4 12.471318649568268 17.369695321171584 36.32617989168618 33.83280613757396 5 12.915887005990054 38.62818625513133 33.063511127342274 15.392415611536341 6 30.880740948258843 36.35875924508814 17.05339197042825 15.707107836224765 7 27.93190175090265 34.79744318199623 19.776482112740407 17.494172954360714 8 27.358276530297598 33.759429220367366 19.150248312204024 19.73204593713101 9 27.670115575744695 16.381597378252508 20.073490655731437 35.87479639027136 10-11 25.43828972215067 27.16214886526943 25.671214864591303 21.728346547988597 12-13 26.733589165549514 25.103990593998283 27.061401612081397 21.101018628370802 14-15 23.200290553198 25.721510149619853 26.785513955139738 24.292685342042407 16-17 21.217496867904213 29.558868045854258 27.098911553626053 22.124723532615473 18-19 21.08052447484515 26.66402185541657 31.292629562847797 20.962824106890483 20-21 22.252702313959148 25.431427001015933 31.533639554844424 20.78223113018049 22-23 21.773892312243355 25.78874337543307 31.56226967565318 20.875094636670394 24-25 21.4218832552942 26.09357676612765 31.728219498147052 20.756320480431096 26-27 21.055862742418903 26.3797084517406 31.73821453328837 20.826214272552118 28-29 20.70003515969458 26.673391506646183 31.882854917061355 20.743718416597883 30-31 20.78925053444616 26.318096838949167 31.768006182089614 21.124646444515054 32-33 20.630154483299773 26.460239775328926 31.695461892468995 21.21414384890231 34-35 21.409532417559515 26.392425513591032 31.35510435101731 20.84293771783214 36-37 21.43110299696064 26.182048820904676 31.29352490165719 21.0933232804775 38-39 20.975278939667298 26.386941960053566 31.418244815058216 21.219534285220924 40-41 21.044029572428318 26.39050899301431 31.479436255997694 21.086025178559677 42-43 21.116405210446718 26.378716968549416 30.9670965667536 21.53778125425027 44-45 20.93365565168559 26.618120441936316 30.913323773255684 21.53490013312241 46-47 21.374994751115114 26.951316044289936 30.3068419762882 21.366847228306753 48-49 21.313856993426203 27.05478958395615 30.0951317297755 21.53622169284215 50-51 21.21868765969928 27.24656973621455 29.89219573859488 21.64254686549129 52-53 21.63681226289925 27.372950349622737 29.48945741885224 21.500779968625768 54-55 21.118911841923016 27.272140423182336 29.583561307288836 22.025386427605813 56-57 21.37631758803166 27.523092750488615 29.328490360694083 21.77209930078564 58-59 21.56019421187445 27.093208287660204 29.37485937688999 21.971738123575356 60-61 21.33779817583232 27.296426308476494 29.78145210165351 21.58432341403768 62-63 21.137776490886996 27.41509811184293 29.677163809706457 21.76996158756362 64-65 20.977928360712145 27.429920336028914 29.542949039751765 22.049202263507183 66-67 21.16044613355929 27.523446078406792 29.213494061078883 22.10261372695503 68-69 21.047728160134675 27.395323388634818 29.400579233458913 22.156369217771598 70-71 21.16077757450753 27.516033384788074 29.184458036183774 22.138731004520622 72-73 21.197980792522326 27.49883819422008 29.020733258480558 22.282447754777035 74-75 21.002590285520537 27.90830527138458 28.755083584183026 22.334020858911856 76-77 20.977458311319353 27.46426527832878 29.185492144847913 22.372784265503952 78-79 21.00704008643895 27.596067128067155 29.04410128749662 22.352791497997277 80-81 21.04499533554173 27.972867487080688 28.89156364486441 22.09057353251317 82-83 21.125792637927226 27.767264112363037 28.805406311646355 22.301536938063386 84-85 20.923659526647135 27.60104181127998 28.753195333974098 22.72210332809879 86-87 21.071391301977062 27.418900442393983 28.85892937333734 22.65077888229161 88-89 20.910491941159844 27.59315282183185 28.513133493400822 22.983221743607483 90-91 20.909915655118276 27.424261179261467 28.884024612472636 22.781798553147617 92-93 21.1039329346061 27.199470787056974 28.79212347633489 22.904472802002037 94-95 20.776245834578962 27.03642632101115 28.93896690664255 23.24836093776734 96-97 20.8253699057174 26.947800339125287 28.8716829758291 23.355146779328212 98-99 20.7392059424271 27.065757403121253 28.50285508001181 23.692181574439843 100-101 20.900119517892275 26.481611354387784 28.800772385162233 23.817496742557708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5127.0 1 3228.5 2 2027.5 3 2678.0 4 2042.5 5 1868.0 6 3835.5 7 7872.0 8 11649.5 9 12135.5 10 8667.5 11 5399.5 12 4714.5 13 4693.0 14 4662.0 15 4423.5 16 4239.5 17 4230.0 18 4370.5 19 4525.5 20 4650.0 21 5121.0 22 5741.5 23 6512.5 24 7428.0 25 8443.5 26 9916.5 27 11834.0 28 13518.5 29 15255.5 30 17814.0 31 20209.5 32 23109.0 33 25752.5 34 27860.0 35 30048.0 36 32820.5 37 35926.5 38 37975.0 39 38460.0 40 39041.5 41 42097.0 42 45066.5 43 47805.0 44 50316.0 45 53173.5 46 54441.0 47 55675.5 48 60988.0 49 61343.5 50 56554.0 51 53044.0 52 51033.0 53 51296.5 54 51491.5 55 48836.0 56 44865.5 57 40851.5 58 37487.5 59 33624.0 60 28858.5 61 24391.0 62 19976.0 63 15622.5 64 12031.5 65 9403.5 66 7130.0 67 5782.5 68 4244.0 69 2600.5 70 1851.5 71 1403.0 72 1201.5 73 1099.5 74 905.0 75 758.0 76 507.0 77 248.5 78 124.5 79 74.5 80 47.0 81 29.5 82 17.5 83 9.5 84 2.0 85 1.5 86 1.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014916234064542169 3 0.0026949498519971147 4 0.0017548510664167258 5 0.12127274333987015 6 0.0 7 0.0 8 3.133662618601296E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.267325237202592E-5 24-25 6.267325237202592E-4 26-27 0.005953958975342463 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010560443024686368 36-37 6.580691499062721E-4 38-39 0.007238760648968994 40-41 0.010779799407988459 42-43 0.005640592713482333 44-45 0.0033216823757173737 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.133662618601296E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.267325237202592E-5 68-69 0.0012534650474405184 70-71 0.0 72-73 3.133662618601296E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.133662618601296E-5 82-83 0.0021622272068348943 84-85 0.005421236330180242 86-87 0.003353019001903387 88-89 0.0 90-91 0.0027889597305551534 92-93 0.0 94-95 0.0 96-97 9.400987855803889E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1595577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.74556872613337 #Duplication Level Percentage of deduplicated Percentage of total 1 72.26911553633313 33.059917915442 2 14.421520728803328 13.194413352696593 3 5.353451315676475 7.34690025249862 4 2.5693542428765928 4.701462843971743 5 1.420858246257432 3.2498984277131346 6 0.8378482748520264 2.2996707503589398 7 0.5504308711295041 1.7625841270968132 8 0.40072488311083365 1.4665110144494722 9 0.2905016762101198 1.1960247954714283 >10 1.6444258587974758 14.01727635790685 >50 0.15282837977148617 4.814492049673545 >100 0.08182490236048306 6.713608371971418 >500 0.00478899872584306 1.4748786593366903 >1k 0.001915599490337224 1.8305320371897256 >5k 0.0 0.0 >10k+ 4.104856050722623E-4 2.8718290442230057 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19422 1.2172399075694875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15523 0.9728768965709584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10936 0.6853946879404754 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4757 0.2981366615337273 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3773 0.2364661811996538 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3354 0.21020608845577493 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2516 0.1576859029680172 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2412 0.15116788472132653 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2022 0.12672531629623643 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.1213980898446142 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1726 0.10817403359411673 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.267325237202592E-5 0.0 12-13 0.0 0.0 0.0 6.267325237202592E-5 0.0 14-15 0.0 0.0 0.0 6.267325237202592E-5 0.0 16-17 0.0 0.0 0.0 9.400987855803889E-5 3.133662618601296E-5 18-19 0.0 0.0 0.0 1.566831309300648E-4 6.267325237202592E-5 20-21 0.0 0.0 0.0 2.1935638330209073E-4 6.267325237202592E-5 22-23 0.0 0.0 0.0 5.327226451622203E-4 6.267325237202592E-5 24-25 0.0 0.0 0.0 0.0014728214307426091 6.267325237202592E-5 26-27 0.0 0.0 0.0 0.003635048637577503 6.267325237202592E-5 28-29 0.0 0.0 0.0 0.010247076762826237 6.267325237202592E-5 30-31 0.0 0.0 0.0 0.027450884538947354 6.267325237202592E-5 32-33 0.0 0.0 0.0 0.06091840130560919 6.267325237202592E-5 34-35 0.0 0.0 0.0 0.1102735875485796 6.267325237202592E-5 36-37 0.0 0.0 0.0 0.1878317373589617 6.267325237202592E-5 38-39 0.0 0.0 0.0 0.3034012147329775 6.267325237202592E-5 40-41 0.0 0.0 0.0 0.4265228190178224 6.267325237202592E-5 42-43 3.133662618601296E-5 0.0 0.0 0.5468554635721121 6.267325237202592E-5 44-45 6.267325237202592E-5 0.0 0.0 0.6847366187905692 6.267325237202592E-5 46-47 6.267325237202592E-5 0.0 0.0 0.8479691045935107 6.267325237202592E-5 48-49 6.267325237202592E-5 0.0 0.0 1.0189417370643974 6.267325237202592E-5 50-51 6.267325237202592E-5 0.0 0.0 1.1965577342867189 6.267325237202592E-5 52-53 6.267325237202592E-5 0.0 0.0 1.368783831805046 6.267325237202592E-5 54-55 6.267325237202592E-5 0.0 0.0 1.5507242834410373 6.267325237202592E-5 56-57 6.267325237202592E-5 0.0 3.133662618601296E-5 1.7565432442307705 6.267325237202592E-5 58-59 6.267325237202592E-5 0.0 6.267325237202592E-5 1.975178885130583 6.267325237202592E-5 60-61 6.267325237202592E-5 0.0 6.267325237202592E-5 2.2077593246831713 6.267325237202592E-5 62-63 6.267325237202592E-5 0.0 6.267325237202592E-5 2.435388577298369 6.267325237202592E-5 64-65 6.267325237202592E-5 0.0 6.267325237202592E-5 2.6605735730710585 6.267325237202592E-5 66-67 6.267325237202592E-5 0.0 6.267325237202592E-5 2.914149552168275 6.267325237202592E-5 68-69 6.267325237202592E-5 0.0 6.267325237202592E-5 3.1895358230909574 6.267325237202592E-5 70-71 6.267325237202592E-5 0.0 6.267325237202592E-5 3.489364662438729 6.267325237202592E-5 72-73 6.267325237202592E-5 0.0 6.267325237202592E-5 3.781986077763718 6.267325237202592E-5 74-75 6.267325237202592E-5 0.0 6.267325237202592E-5 4.072445265881872 6.267325237202592E-5 76-77 6.267325237202592E-5 0.0 6.267325237202592E-5 4.371898065715412 6.267325237202592E-5 78-79 6.267325237202592E-5 0.0 6.267325237202592E-5 4.70563313459645 6.267325237202592E-5 80-81 6.267325237202592E-5 0.0 6.267325237202592E-5 5.063998791659694 6.267325237202592E-5 82-83 6.267325237202592E-5 0.0 6.267325237202592E-5 5.416943212392758 6.267325237202592E-5 84-85 6.267325237202592E-5 0.0 6.267325237202592E-5 5.772739266108749 6.267325237202592E-5 86-87 6.267325237202592E-5 0.0 6.267325237202592E-5 6.1411953168038895 6.267325237202592E-5 88-89 6.267325237202592E-5 0.0 6.267325237202592E-5 6.526165769499059 6.267325237202592E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8785 0.0 39.789448 1 GTATCAA 13740 0.0 34.61271 1 TATCAAC 17070 0.0 27.804775 2 ATCAACG 17210 0.0 27.52073 3 TCAACGC 17305 0.0 27.368788 4 CAACGCA 17585 0.0 26.959198 5 AACGCAG 17970 0.0 26.350195 6 GTACATG 11110 0.0 26.240297 1 TACATGG 11320 0.0 25.207348 2 ACATGGG 11495 0.0 23.828405 3 GAGTACT 11250 0.0 23.62024 12-13 ACGCAGA 20095 0.0 23.445547 7 CGCAGAG 20250 0.0 23.219181 8 CATGGGA 7610 0.0 22.37001 4 GTACTTT 11785 0.0 22.286007 14-15 GCAGAGT 20990 0.0 22.196949 9 ATGGGAT 2900 0.0 22.135532 5 AGTACTT 11400 0.0 21.788813 12-13 AGAGTAC 17760 0.0 20.698303 10-11 ATACCGT 230 9.038558E-8 20.64947 6 >>END_MODULE