FastQCFastQC Report
Thu 26 May 2016
SRR938022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597415
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208281.3038565432276523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156550.9800208461796088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109320.6843556621166071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50060.31338130667359454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.24333063105079142No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33070.20702196987007135No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26810.16783365625088034No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26310.16470359925254238No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.14867770742105213No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.13165019735009376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16570.10373008892491933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92900.038.223831
GTATCAA145050.034.582951
TATCAAC178550.028.0322952
ATCAACG179800.027.787153
TCAACGC181350.027.549654
CAACGCA183150.027.2269845
AACGCAG188100.026.5340926
GTACATG114150.025.2544571
TACATGG114950.024.8103222
GAGTACT111350.024.4251512-13
ACGCAGA211100.023.5434577
ACATGGG117050.023.3927923
CGCAGAG212800.023.2883918
GTACTTT117500.023.02687814-15
AGTACTT112600.022.69843112-13
GCAGAGT217000.022.5085039
CTAGCGG652.9426036E-421.92361628-29
GTACTAG2405.6134013E-921.8755261
CATGGGG37250.021.6946984
CATGGGA79000.020.8801384
Produced by FastQC (version 0.11.5)