##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938022_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1597415 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0717440364589 33.0 31.0 34.0 31.0 34.0 2 32.23046234071922 34.0 31.0 34.0 31.0 34.0 3 32.291426460875854 34.0 31.0 34.0 30.0 34.0 4 35.81316439372361 37.0 35.0 37.0 35.0 37.0 5 35.8412478911241 37.0 35.0 37.0 35.0 37.0 6 35.89531086161079 37.0 37.0 37.0 35.0 37.0 7 35.846906408165694 37.0 36.0 37.0 35.0 37.0 8 35.833251847516145 37.0 36.0 37.0 35.0 37.0 9 37.48320067108422 39.0 38.0 39.0 35.0 39.0 10-11 37.420150367938206 39.0 37.5 39.0 35.0 39.0 12-13 37.32554596019193 39.0 37.0 39.0 34.5 39.0 14-15 38.50724232588276 40.0 38.0 41.0 34.0 41.0 16-17 38.390921895687725 40.0 38.0 41.0 34.0 41.0 18-19 38.57754121502552 40.0 38.0 41.0 34.0 41.0 20-21 38.58948958160528 40.0 38.0 41.0 34.0 41.0 22-23 38.506415677829494 40.0 38.0 41.0 34.0 41.0 24-25 38.394604407746264 40.0 38.0 41.0 34.0 41.0 26-27 38.22289667994855 40.0 38.0 41.0 34.0 41.0 28-29 38.01755680270938 40.0 38.0 41.0 33.5 41.0 30-31 37.755960098033384 40.0 37.0 41.0 33.0 41.0 32-33 37.4521570787804 40.0 36.5 41.0 33.0 41.0 34-35 37.20350222077544 40.0 36.0 41.0 32.5 41.0 36-37 36.94629135196551 39.5 35.0 41.0 31.5 41.0 38-39 36.67337385713794 39.0 35.0 41.0 31.0 41.0 40-41 36.44665694262292 39.0 35.0 41.0 30.0 41.0 42-43 36.23487415605838 39.0 35.0 41.0 30.0 41.0 44-45 35.90141478576325 39.0 35.0 41.0 29.5 41.0 46-47 35.41377131177559 39.0 35.0 40.5 26.5 41.0 48-49 34.956872822654105 38.0 35.0 40.0 24.5 41.0 50-51 34.215495347170275 37.5 33.5 39.5 23.5 40.5 52-53 34.32101927176094 38.0 34.0 40.0 24.0 40.5 54-55 34.44512290168804 38.0 34.0 40.0 23.5 41.0 56-57 34.17393132028934 38.0 34.0 40.0 22.5 41.0 58-59 33.88736427290341 38.0 34.0 40.0 22.0 41.0 60-61 33.50490792937339 37.0 33.5 40.0 20.5 41.0 62-63 33.0218305825349 36.5 33.0 39.5 18.0 41.0 64-65 32.55507147485156 36.0 32.5 39.0 14.0 41.0 66-67 32.06789406635095 35.0 32.0 39.0 9.5 40.0 68-69 31.51708228606843 35.0 31.0 38.0 7.0 40.0 70-71 31.039939214293092 35.0 31.0 37.5 2.0 40.0 72-73 30.47124322733917 35.0 30.0 37.0 2.0 39.0 74-75 29.853478588845103 34.5 29.0 36.5 2.0 39.0 76-77 29.24297536958148 34.0 29.0 36.0 2.0 38.5 78-79 28.57582281373344 34.0 27.5 35.0 2.0 37.5 80-81 27.948482078858653 34.0 26.5 35.0 2.0 37.0 82-83 27.270838197963585 33.0 25.5 35.0 2.0 36.0 84-85 26.66630618843569 33.0 25.0 35.0 2.0 36.0 86-87 26.0274941076677 32.5 23.5 35.0 2.0 35.5 88-89 25.274400202827692 32.0 19.5 34.5 2.0 35.0 90-91 24.50082226597346 31.0 15.0 34.0 2.0 35.0 92-93 23.64787422178958 31.0 4.5 34.0 2.0 35.0 94-95 22.750950128801847 30.5 2.0 34.0 2.0 35.0 96-97 21.610932037072395 29.5 2.0 34.0 2.0 35.0 98-99 20.193750215191418 29.0 2.0 34.0 2.0 35.0 100-101 16.40598686002072 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3909844143239738 1101 2 0.28811106557770927 1101 3 0.038380496048972645 1101 4 0.215210941762642 1101 5 0.15573598672547462 1101 6 0.1650378222071538 1101 7 0.24511398860260414 1101 8 0.11733449869805668 1101 9 0.2910842533836302 1101 10-11 0.19935674964863637 1101 12-13 0.24149982949476367 1101 14-15 0.3339553054596749 1101 16-17 0.4495785973168367 1101 18-19 0.3991427436506285 1101 20-21 0.3822817997270036 1101 22-23 0.34463275217425604 1101 24-25 0.46593927921399114 1101 26-27 0.47851504180363236 1101 28-29 0.3728026349200704 1101 30-31 0.34483858471138973 1101 32-33 0.30891020545309544 1101 34-35 0.3342660971580358 1101 36-37 0.4469634749619473 1101 38-39 0.5555722845205153 1101 40-41 0.5447139353533004 1101 42-43 0.562622606465915 1101 44-45 0.4979574493767558 1101 46-47 0.6933449631552762 1101 48-49 0.6994323583600419 1101 50-51 0.5893755775433434 1101 52-53 0.7184777199207204 1101 54-55 0.700254030716323 1101 56-57 0.7989066858238658 1101 58-59 0.9593181115293703 1101 60-61 0.942249807216335 1101 62-63 0.8077682108310782 1101 64-65 0.6834777697414935 1101 66-67 0.7414651399778407 1101 68-69 1.215791897634336 1101 70-71 1.295732583779305 1101 72-73 1.404220404212822 1101 74-75 1.5526846125196094 1101 76-77 1.5163171946633156 1101 78-79 1.4698324221382926 1101 80-81 1.6276443714152578 1101 82-83 1.614870095982024 1101 84-85 1.6306825550620658 1101 86-87 1.8480502287775344 1101 88-89 2.230290552854573 1101 90-91 2.701245977951668 1101 92-93 2.807109782995667 1101 94-95 2.9657449317968307 1101 96-97 2.994497162523796 1101 98-99 3.3514877124282556 1101 100-101 3.5194370349756916 1102 1 0.26897596442580607 1102 2 0.20149792615439566 1102 3 0.2165790854882843 1102 4 0.16933910416227604 1102 5 0.17902538732134587 1102 6 0.19404016191999318 1102 7 0.073688783667329 1102 8 0.18196780341619956 1102 9 0.33248202139857597 1102 10-11 0.2971314975824484 1102 12-13 0.34043265112111953 1102 14-15 0.43709961019465027 1102 16-17 0.37661490494699024 1102 18-19 0.3767774454634889 1102 20-21 0.3667605824030318 1102 22-23 0.38104474362336305 1102 24-25 0.2927113171206983 1102 26-27 0.3744752498529138 1102 28-29 0.40769802114100884 1102 30-31 0.2956154030385818 1102 32-33 0.43028575461230645 1102 34-35 0.44889182726627297 1102 36-37 0.5239972577221366 1102 38-39 0.4692335143005124 1102 40-41 0.42880388805406255 1102 42-43 0.5832373281410881 1102 44-45 0.5634997072838033 1102 46-47 0.5318509134321658 1102 48-49 0.6467282226230395 1102 50-51 0.6476680921455014 1102 52-53 0.6403548765804814 1102 54-55 0.8119458619203499 1102 56-57 0.8971662087917593 1102 58-59 0.7798284719124737 1102 60-61 0.9412449089685992 1102 62-63 0.8628575070656481 1102 64-65 0.9406054776645263 1102 66-67 0.9219781935555815 1102 68-69 1.084406080670579 1102 70-71 1.164193591370747 1102 72-73 1.277753033949061 1102 74-75 1.2697796654577154 1102 76-77 1.4517714187707718 1102 78-79 1.7885686405563774 1102 80-81 1.730137259698175 1102 82-83 1.9741944407782697 1102 84-85 1.9421606139420327 1102 86-87 1.9451281125998499 1102 88-89 2.160275908608753 1102 90-91 2.317775460016673 1102 92-93 2.693630343853446 1102 94-95 3.0626999386139815 1102 96-97 3.126228045050013 1102 98-99 3.0314873757789265 1102 100-101 3.1606378631330436 1103 1 0.3877928591624311 1103 2 0.36929779629539894 1103 3 0.24736138382910866 1103 4 0.19866763516304786 1103 5 0.24243577849093612 1103 6 0.27455682103197177 1103 7 0.277330552526486 1103 8 0.21896944911350147 1103 9 0.32258333591460797 1103 10-11 0.4016720703504504 1103 12-13 0.5404297925898334 1103 14-15 0.6680975234668125 1103 16-17 0.6862979754996275 1103 18-19 0.5600317608248275 1103 20-21 0.6158611659517774 1103 22-23 0.63651985194128 1103 24-25 0.6693473158302794 1103 26-27 0.7435749350061158 1103 28-29 0.870992437612685 1103 30-31 0.7660888166558095 1103 32-33 0.7427926457145162 1103 34-35 0.7288829249831252 1103 36-37 0.8791738537014098 1103 38-39 0.8868383917716187 1103 40-41 0.9992074718355752 1103 42-43 1.1330594866588939 1103 44-45 1.0491212949120836 1103 46-47 1.1866567170674074 1103 48-49 1.0807757804164169 1103 50-51 1.07214365374481 1103 52-53 1.1712249726679715 1103 54-55 1.0412123219560385 1103 56-57 1.1152170171435785 1103 58-59 1.153695778890146 1103 60-61 1.094159998338533 1103 62-63 1.1215137523439722 1103 64-65 1.3940400030054079 1103 66-67 1.5760877406417109 1103 68-69 1.585872098859003 1103 70-71 1.6197393462476057 1103 72-73 1.8543657560467324 1103 74-75 1.8605686609289869 1103 76-77 1.86795111421468 1103 78-79 2.0604680529240156 1103 80-81 2.159157886043218 1103 82-83 2.0325391061463485 1103 84-85 2.2084756812604454 1103 86-87 2.3869467918767207 1103 88-89 2.8216869613241506 1103 90-91 3.066992426624946 1103 92-93 3.4210326331286467 1103 94-95 3.5686354746498523 1103 96-97 3.8408159469288847 1103 98-99 3.9704638582336713 1103 100-101 4.033553687553887 1104 1 0.2395073653919937 1104 2 0.18948964458089534 1104 3 0.19870468935301489 1104 4 0.1432521476405384 1104 5 0.17426348255943935 1104 6 0.20777377130577435 1104 7 0.1951518616521426 1104 8 0.20501818298831864 1104 9 0.31302032367600674 1104 10-11 0.26593757073635516 1104 12-13 0.2655189175117343 1104 14-15 0.47709270888339717 1104 16-17 0.43696687182070093 1104 18-19 0.4125952508465076 1104 20-21 0.4084445023478267 1104 22-23 0.3732117553555909 1104 24-25 0.35672097895645294 1104 26-27 0.46722887304132144 1104 28-29 0.5493819410857981 1104 30-31 0.5763262380972449 1104 32-33 0.393018673866969 1104 34-35 0.36200431863963445 1104 36-37 0.4727550216972958 1104 38-39 0.4558449704771874 1104 40-41 0.5511296299450237 1104 42-43 0.5074264240693154 1104 44-45 0.513258161390084 1104 46-47 0.44703379818027145 1104 48-49 0.5128772909460153 1104 50-51 0.6046384165071359 1104 52-53 0.5430118814113953 1104 54-55 0.6892405479106856 1104 56-57 0.8538604807034247 1104 58-59 0.7886621503113744 1104 60-61 0.9379322795690115 1104 62-63 0.9293861475073371 1104 64-65 1.0847048565465158 1104 66-67 1.1242556262677894 1104 68-69 1.0356828232516655 1104 70-71 1.2895214036550868 1104 72-73 1.5614314328448486 1104 74-75 1.6226437096261073 1104 76-77 1.6289970916486176 1104 78-79 1.695055873130567 1104 80-81 1.874167625467674 1104 82-83 1.861461521523612 1104 84-85 2.2090343199461735 1104 86-87 2.2378472292320097 1104 88-89 2.668868041113928 1104 90-91 2.6129790486985236 1104 92-93 2.812608949788576 1104 94-95 3.0946875162537317 1104 96-97 3.5715006468443526 1104 98-99 3.72120442201771 1104 100-101 3.787138898329733 1105 1 0.27271515898035403 1105 2 0.22777268608493983 1105 3 0.19133024175320656 1105 4 0.1188030292029012 1105 5 0.13211629733262953 1105 6 0.25121938171747615 1105 7 0.24631816637962345 1105 8 0.22331534771367245 1105 9 0.24637846123314944 1105 10-11 0.1705101534635034 1105 12-13 0.25375032573357714 1105 14-15 0.3550946527246168 1105 16-17 0.5406499193992502 1105 18-19 0.3538069624898128 1105 20-21 0.36219626775048397 1105 22-23 0.3062969198987062 1105 24-25 0.15996780123165877 1105 26-27 0.30968054277055757 1105 28-29 0.37938429495601866 1105 30-31 0.33524626725485973 1105 32-33 0.47715624619152663 1105 34-35 0.4491643362556914 1105 36-37 0.3596211851925659 1105 38-39 0.5651959097473451 1105 40-41 0.594828091576943 1105 42-43 0.6659459156333511 1105 44-45 0.8166841815270658 1105 46-47 0.7940269188047324 1105 48-49 0.6760946518778468 1105 50-51 0.5751482192376258 1105 52-53 0.5792793266269669 1105 54-55 0.6775345994528479 1105 56-57 0.6933132438433347 1105 58-59 0.6692081722707002 1105 60-61 0.6602389964515751 1105 62-63 0.544438767896402 1105 64-65 0.6071892526649094 1105 66-67 0.8879350291440673 1105 68-69 1.0320035722379863 1105 70-71 1.1075296497939746 1105 72-73 1.2700082525383891 1105 74-75 1.4421675444603501 1105 76-77 1.3882656454611322 1105 78-79 1.5220767846098582 1105 80-81 1.573328584631824 1105 82-83 1.8337993702380935 1105 84-85 1.7269396791771428 1105 86-87 1.813966076898641 1105 88-89 2.3279942237438824 1105 90-91 2.516107797806331 1105 92-93 2.59041081466124 1105 94-95 2.773553558258925 1105 96-97 3.244824083798594 1105 98-99 3.5694287686288995 1105 100-101 3.5088100702533715 1106 1 0.16479768699645803 1106 2 0.18340404982824055 1106 3 0.17246869476983306 1106 4 0.01793686026943675 1106 5 0.1601510155634287 1106 6 -0.04411265715216928 1106 7 0.029749874982350377 1106 8 0.17194156834627705 1106 9 0.14317972631330633 1106 10-11 0.09827580391661428 1106 12-13 0.2439666520575443 1106 14-15 0.3616180945288363 1106 16-17 0.21252236012839631 1106 18-19 0.2312806140984165 1106 20-21 0.2788373727301021 1106 22-23 0.1689550921437828 1106 24-25 0.18157288656798443 1106 26-27 0.1571949044679215 1106 28-29 0.26514328348751093 1106 30-31 0.17676616516694565 1106 32-33 0.2074984402479103 1106 34-35 0.2283493304038018 1106 36-37 0.2734487673944841 1106 38-39 0.004841044042983356 1106 40-41 0.33660938876058566 1106 42-43 0.2286110118693685 1106 44-45 0.4320944540493912 1106 46-47 0.5891162457332513 1106 48-49 0.5103886579352235 1106 50-51 0.5026316984462937 1106 52-53 0.4461008276290883 1106 54-55 0.038068669955457324 1106 56-57 0.2819742035531547 1106 58-59 0.4364731065002374 1106 60-61 0.6086012864610737 1106 62-63 0.3232827741026867 1106 64-65 0.5787342556280848 1106 66-67 0.7292903892064899 1106 68-69 0.34195528298490885 1106 70-71 0.507182035667249 1106 72-73 0.827158352750132 1106 74-75 0.9673853895952398 1106 76-77 1.1436421616662429 1106 78-79 1.1153734679437406 1106 80-81 1.2728951279075105 1106 82-83 1.4733754879787 1106 84-85 1.7159297890377978 1106 86-87 1.3363193999675893 1106 88-89 1.7704461801808158 1106 90-91 1.9336151461709292 1106 92-93 2.4657637148455827 1106 94-95 2.7328397469071533 1106 96-97 2.959859790745522 1106 98-99 3.2449381658992102 1106 100-101 3.3052905237524435 1107 1 0.3475793223003194 1107 2 0.23295759643780656 1107 3 0.06788589602295048 1107 4 0.07743425634907908 1107 5 0.09968035325864832 1107 6 0.22635318936201543 1107 7 0.09981926713138023 1107 8 0.05910441787916909 1107 9 0.18126598744755995 1107 10-11 0.07852745244043291 1107 12-13 0.08907887730658359 1107 14-15 0.2635402231227175 1107 16-17 0.3879075692254048 1107 18-19 0.23741677606493283 1107 20-21 0.4040219359191255 1107 22-23 0.2205649163565937 1107 24-25 0.2616525528849607 1107 26-27 0.39371014929708537 1107 28-29 0.4300886239032522 1107 30-31 0.48690199645398735 1107 32-33 0.4213684586663291 1107 34-35 0.3880113803133014 1107 36-37 0.45893450160760096 1107 38-39 0.45931395323536606 1107 40-41 0.575143186296664 1107 42-43 0.5831757604266201 1107 44-45 0.45704053813295076 1107 46-47 0.6491316822554296 1107 48-49 0.6323729225019008 1107 50-51 0.46393714592132795 1107 52-53 0.518951934502816 1107 54-55 0.6514373985073192 1107 56-57 0.4152699804585893 1107 58-59 0.4913579571204778 1107 60-61 0.6318113033055184 1107 62-63 0.7457285098175888 1107 64-65 0.922010596089315 1107 66-67 0.8114956458549187 1107 68-69 0.9061861607945083 1107 70-71 1.177001107786424 1107 72-73 1.3416703442131137 1107 74-75 1.4372567350635954 1107 76-77 1.624803900751477 1107 78-79 1.7115502933774636 1107 80-81 1.8465059672629884 1107 82-83 1.8376902269693467 1107 84-85 2.1134749271470206 1107 86-87 2.2852081219925076 1107 88-89 2.626187440098491 1107 90-91 2.6969910586982664 1107 92-93 3.0070085786641236 1107 94-95 3.0800190798732245 1107 96-97 2.9611864796837146 1107 98-99 3.2265625313447934 1107 100-101 3.313627906344987 1108 1 0.22803002997046917 1108 2 0.12815379592723986 1108 3 0.050948010999604776 1108 4 0.13133166498379012 1108 5 0.17543986939671896 1108 6 0.1664115829770978 1108 7 0.1374394980222533 1108 8 0.08608743141772379 1108 9 0.09572868296341852 1108 10-11 0.1521377166723923 1108 12-13 0.13497646258283424 1108 14-15 0.23528114434229508 1108 16-17 0.3127975504232765 1108 18-19 0.29200937104796765 1108 20-21 0.2508869732940937 1108 22-23 0.18130497644868626 1108 24-25 0.21582596391792208 1108 26-27 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-1.8835382131307732 2316 86-87 -1.8099707070060838 2316 88-89 -1.9857522518461792 2316 90-91 -2.0427770219232926 2316 92-93 -2.4625167209822827 2316 94-95 -2.6986237380586644 2316 96-97 -2.9404130420377363 2316 98-99 -3.002516830065712 2316 100-101 -2.871289110865611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3696.0 3 955.0 4 910.0 5 1039.0 6 1215.0 7 1514.0 8 1991.0 9 2529.0 10 3265.0 11 4317.0 12 5952.0 13 8047.0 14 9532.0 15 12555.0 16 16636.0 17 22424.0 18 21272.0 19 14153.0 20 13674.0 21 14105.0 22 14997.0 23 16476.0 24 18599.0 25 21079.0 26 24386.0 27 28590.0 28 33603.0 29 39839.0 30 47916.0 31 58032.0 32 72260.0 33 93168.0 34 126440.0 35 186515.0 36 227482.0 37 264978.0 38 153561.0 39 9712.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68174128933993 18.705578872302343 14.472782292296838 21.139897546060883 2 15.742297094188377 20.79239729458918 40.1249373747495 23.340368236472948 3 19.210052285715 26.31240401550283 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25.716696066129536 31.65647314795127 20.803953442273663 24-25 21.378140237638252 26.054074979829313 31.853397946348476 20.714386836183955 26-27 21.049432187059296 26.32988427019885 31.882991793716396 20.73769174902546 28-29 20.74693396337196 26.640237610988727 32.00301867413194 20.60980975150738 30-31 20.872299409778243 26.193721002475122 31.87479332217691 21.05918626556973 32-33 20.60137727684229 26.453848762589626 31.744756299157984 21.2000176614101 34-35 21.419409267901308 26.431750895167255 31.411148112679903 20.737691724251533 36-37 21.45145320053585 26.124729679711557 31.35183003625564 21.07198708349695 38-39 20.935900827636516 26.392822871383686 31.497682398596133 21.17359390238366 40-41 21.05481818585974 26.39506262511404 31.54927575525518 21.00084343377104 42-43 21.15874821780895 26.332721998232994 31.068763614937783 21.439766169020274 44-45 20.994518203939343 26.59179793581938 30.99526639036987 21.418417469871407 46-47 21.3654065734655 26.967056905977195 30.402285769388637 21.26525075116867 48-49 21.36584034096228 27.021755349608128 30.182945060752616 21.42945924867698 50-51 21.184409560827056 27.236561347972433 29.99623086632473 21.58279822487578 52-53 21.553046228187952 27.34359861169243 29.643628695655167 21.459726464464453 54-55 21.13075001408742 27.195038724744393 29.781519813169545 21.892691447998647 56-57 21.397926041870473 27.448687145372723 29.563310506750145 21.590076306006655 58-59 21.589959992737338 26.982707345934475 29.611322243161514 21.81601041816668 60-61 21.342106317650654 27.142437652456586 30.058709872925714 21.456746156967046 62-63 21.187404244875164 27.21386474727534 30.002541991381023 21.596189016468472 64-65 21.097879374143016 27.283038123681262 29.882166582142165 21.73691592003356 66-67 21.17730546183738 27.340615282326304 29.565674961794496 21.91640429404182 68-69 21.176123295917527 27.23110408923446 29.70620082699397 21.886571787854038 70-71 21.28447156668064 27.359894911596193 29.533354016247255 21.82227950547591 72-73 21.362020722890662 27.30869143003473 29.416981650681112 21.912306196393498 74-75 21.15673518887041 27.822600462866877 29.152286207374758 21.868378140887963 76-77 21.319900556380915 27.489643376550365 29.25294577618174 21.937510290886987 78-79 21.4303133057999 27.631566178387995 29.05996364284084 21.878156872971267 80-81 21.457784306101583 27.96152254369208 28.921924962260736 21.6587681879456 82-83 21.63343121356883 27.83162929895629 28.763860279616075 21.7710792078588 84-85 21.47529529321461 27.74776393572356 28.683010419314485 22.093930351747343 86-87 21.662433664356826 27.61819592091746 28.690302616364782 22.02906779836093 88-89 21.627968939959167 27.648614893878822 28.54327707163215 22.180139094529867 90-91 21.650591039938483 27.64020486796236 28.615168720502016 22.094035371597144 92-93 22.009525040877286 27.33322960745599 28.611718874488663 22.04552647717806 94-95 21.813290516989813 27.18564366665873 28.701269586410653 22.299796229940796 96-97 21.991258637261623 27.03723532487375 28.56697380408953 22.4045322337751 98-99 21.945799459691475 27.21814369677102 28.14538425679088 22.69067258674663 100-101 22.70357176890708 26.286437409408027 28.328675213032206 22.681315608652692 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5629.0 1 3569.0 2 2233.5 3 2838.5 4 2085.5 5 1888.0 6 3889.0 7 7883.0 8 12020.5 9 12662.5 10 8890.0 11 5512.0 12 4746.5 13 4675.0 14 4652.0 15 4516.0 16 4320.0 17 4268.0 18 4411.0 19 4655.0 20 4838.5 21 5157.5 22 5848.0 23 6661.0 24 7509.0 25 8657.5 26 10213.5 27 12017.0 28 13675.0 29 15658.5 30 18115.5 31 20374.0 32 23351.0 33 26049.5 34 28113.0 35 30111.5 36 32780.0 37 36009.0 38 37969.0 39 38376.5 40 39187.5 41 41891.0 42 44657.0 43 47580.5 44 50367.0 45 52990.5 46 54174.5 47 55655.0 48 60996.0 49 61235.0 50 56140.0 51 52474.5 52 50281.0 53 50569.0 54 50703.0 55 47872.0 56 43987.0 57 40713.5 58 37464.0 59 33115.5 60 28666.0 61 24523.5 62 20181.5 63 15862.0 64 12260.0 65 9751.0 66 7447.5 67 5951.5 68 4461.0 69 2886.0 70 2126.0 71 1681.0 72 1379.5 73 1171.0 74 918.5 75 778.0 76 566.0 77 313.5 78 171.0 79 87.5 80 52.0 81 33.0 82 19.0 83 14.5 84 10.5 85 7.5 86 9.5 87 6.5 88 3.0 89 2.5 90 1.5 91 0.0 92 0.5 93 0.5 94 1.0 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4530444499394334 2 0.03849970107955666 3 0.050268715393307316 4 0.055965419130282364 5 0.08119367853688617 6 0.08044246485728504 7 0.04319478657706357 8 0.0370598748603212 9 0.04313218543709681 10-11 0.047076057255002614 12-13 0.04228707004754557 14-15 0.035964354910902926 16-17 0.037153776570271344 18-19 0.045104121346049714 20-21 0.054901199750847465 22-23 0.0457301327457173 24-25 0.06504258442546239 26-27 0.05599671970026574 28-29 0.04303828372714667 30-31 0.04551102875583365 32-33 0.0446659133662824 34-35 0.04920449601387241 36-37 0.04369559569679764 38-39 0.02488395313678662 40-41 0.02328762406763427 42-43 0.022786814947900202 44-45 0.013740950222703555 46-47 0.025259559976587173 48-49 0.025384762256520692 50-51 0.01446086333232128 52-53 0.013678349082736798 54-55 0.015337279291855905 56-57 0.014241759342437625 58-59 0.012833233693185554 60-61 0.01496167245205535 62-63 0.01514947587195563 64-65 0.015337279291855905 66-67 0.00826335047561216 68-69 0.011549910323866997 70-71 0.01774742318057612 72-73 0.015274678151889145 74-75 0.012238522863501345 76-77 0.008012945915745126 78-79 0.012864534263168933 80-81 0.017027510070958395 82-83 0.01408525649252073 84-85 0.011831615453717413 86-87 0.0028796524384709045 88-89 0.027763605575257526 90-91 0.03029895174391126 92-93 0.01605719240147363 94-95 0.0323647893628143 96-97 0.025666467386371107 98-99 0.02281811551788358 100-101 0.14717528006184993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1597415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.796157249968665 #Duplication Level Percentage of deduplicated Percentage of total 1 73.12959665460804 34.22184104675821 2 14.137827506706993 13.231919983535859 3 5.14727623705689 7.226182445950238 4 2.443150510898404 4.57320221973372 5 1.3144771893420675 3.075624062697411 6 0.8256647402609781 2.318276221260436 7 0.560724273398753 1.8367818890289729 8 0.37201316153101754 1.3927029124850794 9 0.274534387738423 1.1562438941218 >10 1.5700692581165026 13.76475988174954 >50 0.14335727834659903 4.637747993986819 >100 0.07472356745698733 6.331132399650182 >500 0.004569346414314145 1.4825817101445868 >1k 0.0014783179575722234 1.454218074305306 >5k 1.3439254159747485E-4 0.31483006876150854 >10k+ 4.031776247924245E-4 2.981955195830389 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20828 1.3038565432276523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15655 0.9800208461796088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10932 0.6843556621166071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5006 0.31338130667359454 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3887 0.24333063105079142 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3307 0.20702196987007135 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2681 0.16783365625088034 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2631 0.16470359925254238 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2375 0.14867770742105213 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2103 0.13165019735009376 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1657 0.10373008892491933 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.26011399667588E-5 0.0 14-15 0.0 0.0 0.0 6.26011399667588E-5 0.0 16-17 0.0 0.0 0.0 1.252022799335176E-4 0.0 18-19 0.0 0.0 0.0 2.504045598670352E-4 0.0 20-21 0.0 0.0 0.0 3.756068398005528E-4 0.0 22-23 0.0 0.0 0.0 0.0010642193794348997 0.0 24-25 0.0 0.0 0.0 0.0027544501585373866 0.0 26-27 0.0 0.0 0.0 0.004914189487390565 0.0 28-29 0.0 0.0 0.0 0.011393407473950101 0.0 30-31 0.0 0.0 0.0 0.027231495885540073 0.0 32-33 0.0 0.0 0.0 0.06062920405780589 0.0 34-35 0.0 0.0 0.0 0.10842517442242623 0.0 36-37 0.0 0.0 0.0 0.18439165777208805 0.0 38-39 0.0 0.0 0.0 0.29776232225188826 0.0 40-41 0.0 0.0 0.0 0.4214308742562202 0.0 42-43 0.0 0.0 0.0 0.539559225373494 0.0 44-45 0.0 0.0 0.0 0.6752784968214272 0.0 46-47 0.0 0.0 0.0 0.8333463752374931 0.0 48-49 0.0 0.0 0.0 0.9973613619504011 0.0 50-51 0.0 0.0 0.0 1.1784664598742343 0.0 52-53 0.0 0.0 0.0 1.3472391332246159 0.0 54-55 0.0 0.0 0.0 1.5256836826998619 0.0 56-57 0.0 0.0 0.0 1.7286052779021106 0.0 58-59 0.0 0.0 0.0 1.9462381409965475 0.0 60-61 0.0 0.0 0.0 2.1780501622934554 0.0 62-63 0.0 0.0 0.0 2.401442330264834 0.0 64-65 0.0 0.0 0.0 2.6274637461148167 0.0 66-67 0.0 0.0 0.0 2.877555300282018 0.0 68-69 0.0 0.0 0.0 3.156380777693962 0.0 70-71 0.0 3.13005699833794E-5 0.0 3.4506374361077117 0.0 72-73 0.0 6.26011399667588E-5 0.0 3.7446123893916106 0.0 74-75 0.0 6.26011399667588E-5 0.0 4.033203644638369 0.0 76-77 0.0 6.26011399667588E-5 0.0 4.333751717618778 0.0 78-79 0.0 6.26011399667588E-5 0.0 4.671766572869291 0.0 80-81 0.0 6.26011399667588E-5 0.0 5.030408503738853 0.0 82-83 0.0 6.26011399667588E-5 0.0 5.386859394709578 0.0 84-85 0.0 6.26011399667588E-5 0.0 5.737801385363227 0.0 86-87 0.0 6.26011399667588E-5 0.0 6.0982274487218415 6.26011399667588E-5 88-89 0.0 6.26011399667588E-5 0.0 6.476338334121064 6.26011399667588E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9290 0.0 38.22383 1 GTATCAA 14505 0.0 34.58295 1 TATCAAC 17855 0.0 28.032295 2 ATCAACG 17980 0.0 27.78715 3 TCAACGC 18135 0.0 27.54965 4 CAACGCA 18315 0.0 27.226984 5 AACGCAG 18810 0.0 26.534092 6 GTACATG 11415 0.0 25.254457 1 TACATGG 11495 0.0 24.810322 2 GAGTACT 11135 0.0 24.42515 12-13 ACGCAGA 21110 0.0 23.543457 7 ACATGGG 11705 0.0 23.392792 3 CGCAGAG 21280 0.0 23.288391 8 GTACTTT 11750 0.0 23.026878 14-15 AGTACTT 11260 0.0 22.698431 12-13 GCAGAGT 21700 0.0 22.508503 9 CTAGCGG 65 2.9426036E-4 21.923616 28-29 GTACTAG 240 5.6134013E-9 21.875526 1 CATGGGG 3725 0.0 21.694698 4 CATGGGA 7900 0.0 20.880138 4 >>END_MODULE